Objective To investigate efficient examination for early diagnosis of otitis meida woth effusion in pre-school children.Methods 169 cases (338 ears) with otitis media effusion in preschool children in our clinic were analyzed . All the data were analyzed retrospectively .All the pediatric patients complained symptoms related to rhinosinusitis and pediat-ric snoring while they had not been found hearing impairment .All the pediatric patients were diagnosed due to the history , special physical examination and acoustic impedance .And all the patients were divided into 3 groups:group 1 with rhinosi-nusitis, group 2 with pediatric snoring , and group 3 with rhinosinusitis and pediatric snoring .The incidence rates of otitis media with effusion were analyzed among the three groups and control group .Results The incidence rates for group1, group 2, group 3 and contrl group were 55.96%、48.08%、63.24%、11.19%respectively.According toχ2 test, there were signif-icant difference between group 3 and control group ( P<0.001 ) , respectively .But there was no difference among the three experimental groups(P>0.05).Conclusion Preschool children are high -risk population, which are attacked by otitis media with effusion .The primary factors include rhinosinusitis and pediatric snoring .Detailed history , careful special physi-cal examination and acoustic impedance are most useful for diagnosis .All the parents , kindergareners and physicians should pay attention to early symptoms , otherwise early diagnosis and early treatment will be missed .

To observe whether there is an interaction between hepatitis virus B (HBV) and tumor suppressor p53, plasmid PCMVp53 was transfected or cotransfected with pCMVHBV a (wild type HBV) or PCMVHBV b (mutation type HBV) into the hepatoma cell line 7721 by phosphate calcium precipitation. Apoptosis cells were labeled by annexin Ⅴ FITC and detected by flow cytometry. Another experiment was performed by cotransfecting the cells with reporter plasmid p21 luc in each group mentioned above, then the luciferase activity was measured. The results showed that the cells transfected by pCMVp53 alone exhibited high luciferase activity and high apoptosis rate. Meanwhile, the luciferase activity and apoptosis rate were further higher in cells cotransfected by pCMVp53 and PCMVHBV a , but remained unchanged in cells cotransfected by PCMVp53 and PCMVHBV b. The results indicated that P53 could induce of 7721 cell apoptosis by activating p21 transcription, and such effect could be enhanced by HBV.

OBJECTIVE:To observe therapeutic efficacy and safety of carboprost tromethamine in the treatment of postpartum hemorrhage with two routes of administration. METHODS:285 patients with postpartum hemorrhage were randomly divided into control group(143 cases)and trial group(142 cases). Control group was given Carboprost tromethamine injection 250 μg on del-toid of arm;trial group was given same dose of Carboprost tromethamine injection via cervix uteri. Both groups received medicine after third stage of labor. The therapuetic efficacy,the amount of endometrorrhagia and colporrhagia within 2 h,the incidence of ADR after labor were observed in 2 groups. RESULTS:After treatment,the amount of postpartum hemorrhage was smaller than 400 ml in 2 groups and didn't exceed the standard,without statistical significance(P>0.05). The amount of endometrorrhagia in trial group was significantly higher than in control group,with statistical significance(P<0.05);the amount of colporrhagia in tri-al group was significantly lower than in control group,with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between trial group(4.90%)and control group(6.31%)(P>0.05). CONCLUSIONS:The intramuscular injection is selected for the prevention of postpartum hemorrhage caused by uterine inertia;the cervical injection is selected for the prevention of postpartum hemorrhage caused for the other patients.

Objective To investigate the effect of the increased pulse pressure on cerebrovascular hemodynamic index.Methods From January 2014 to October 2016,the application of the method of cross-sectional study,6 006 consecutive health subjects examined and detected with ultrasound noninvasive cerebral hemodynamic index (CVHI) at the Physical Examination Center,Pu′er People′s Hospital,Yunnan Province were enrolled.In order to control the influence of age and gender on the results,according to age and gender in the increased pulse pressure group (≥60 mmHg),and the same sex and age difference of not more than 1 years old were selected as the inclusion criteria in the normal pulse pressure group (60 mmHg).Finally,1 115 subjects were enrolled as a control group for comparative analysis.The study population was divided into an increased pulse pressure group (n=590) and a normal pulse pressure group (n=1 115) in a mixed manner.The differences of CVHI index and its integral score of both groups were compared.The correlation of the increased pulse pressure level and changes of cerebrovascular hemodynamic accumulative scores were analyzed.Results The minimum velocity,mean velocity,and critical pressure in the increased pulse pressure group were significantly lower than those in the normal pulse pressure group (P<0.05),while the differences of the peripheral vascular resistance,characteristic impedance,pulse wave velocity,dynamic resistance,diastolic blood pressure,and critical pressure were significant higher than those in the normal pulse pressure group.There were significant differences (P<0.01).The integral values of cerebral hemodynamics in the increased pulse pressure group and the normal pulse pressure group were 76±22 and 88±19 respectively.There was significant difference (P<0.01).The proportion of the integral value <75 was 34.4% (203/590) and 15.6% respectively (174/1 115,P<0.01).With the increase of pulse pressure level,the integral value of cerebral hemodynamics showed a significant downward trend,there was a significant negative correlation between the two (r=-0.312,P<0.01).Conclusion The increased pulse pressure may result in the changes of cerebral hemodynamic indexes and its integral values.There is a significant negative correlation between the pulse pressure level and the integral value of cerebral hemodynamics.

Objective To determine the mechanism underlying the mandibular angle enlargement and to provide a morphological basis for correcting the enlarged mandibular angle. Methods By axial computed tomography, it is possible to measure the width and length of the masseter and the medial pterygoid, the gonial angle, and the vertical height of the posterior mandible. The date were initially analysed by using a simple correctionials and indpendent sample T test. Results The distance between two Go points and gonial angle were significantly related with the thickness of masseter muscle, but the thickness of the medial pterygoid was on the contrary. There was significant differences between the group of prominence and the normal control in the gonial angle, the distance between two Go points and thickness of masseter . But there was no difference between the the two groups in the thickness of medial pterygoid. Conclusion The thickness of the masseter muscle is an important factor that affects the lower facial morphology. The hypertrophy of masserter muscle may cause the mandibular angle enlargement.

Objective To observe the effectiveness of electrolyzed oxidizing water(EOW) in disinfections of endoscopes and compared with that of 2% glutaraldehyde. Methods (1) Disinfections of bacteri-a:Specimens were obtained immediately from endoscopes after being used, disinfected and washed out for bacterial culture. Endoscopes were immersed in either EOW or 2% glutaraldehyde for 1 , 3 and 5 minutes. Each time group consisted of 30 cases. (2 ) Effectiveness on disinfections of H. Pylori: endoscopes either from patients with H. Pylori infection or artificially contaminated with H. Pylori were washed out and exposed for 1 ,3 and 5 minutes in each of the disinfectants. PCR was used to detect DNA of H. Pylor. (3) Effectiveness of disinfections of HBV : Endoscopes were contaminated with HBV-positive serum, and the exposure time to both disinfectants were 1,3,5 and 10 minutes respectively. Thirty samples were studied in each time groups. Results ( 1 ) After manual cleaning and disinfections for 1, 3 and 5min in EOW, no vegetative bacteria were found. In 2% glutaraldehyde processing group, 5/30, 4/30 and 1/30 specimens remained bacteria positive, respectively. (2)There was no positive specimens found after 3 and 5 minutes' exposure in EOW in the group of H. Pylori, but 2/30 positive specimens were found in the 1 minute group. While 3/30, 4/30 and 4/30 positive specimens were found after exposure in 2% glutaraldehyde. (3) In the group of HBV, there is no positive case found after exposure in EOW, while in 2% glutaraldehyde groups, there were 2,5 ,4 and 5 cases remained positive in 1 ,3,5 and 10 minutes group respectively. Conclusion EOW is superior to 2% glutaraldehyde in disinfections of Hp or HVB with high-speed and thoroughness, and worthy to be recommended in disinfections of endoscopes.

Objective To investigate correlation between the expression of TIG1 and the prognostic significance in acute leukemia (AL).Methods The TIG1 expression level of 75 AL and 20 normal control (NC) were measured by Real-Time Quantitative PCR(RT-QT-PCR).Results TIG1 gene expressed at a higher level in NC(0.0609±0.0281 ) than that in de novo AL(0.0057±0.0035)(U=-6.321,P=0.000).The expression of TIG1 in complete remission patients(AL-CR)(0.0409±0.0244)were lower than that in NC too(U=-2.582,P=0.01).The expression of TIG1 in AL-CR patients (83.3 ％) were higher than that in de novo AL (53.1％)(U=-6.366,P=0.000).In AL patients,the CR rate of high TIG1 level cases were higher than that in low level cases (x2=4.563,P=0.033).Conclusions Reduced expression of TIG1 may play an important role in the pathogenesis of AL.TIG1 can serve as a marker of poor prognosis in AL.

Two hundred and thirty-five patients of both sexes (123 male,112 female) aged 18-54 yr,weighing 35-62 kg underwent repair of tetralogy of Fallot from January 1996 to July 2009 in Fuwai hospital.Direct BP, ECG,CVP,SpO2,naso-pharyngeal temperature and TEE were continuously monitored during operation.Anesthesia was induced with midazolam and/or etomidate, fentanyl and pipecuronium and maintained with isoflurane/sevoflurane inhalation and intermittent iv boluses of fentanyl and pipecuronium. The total amount of fentanyl administered ranged from 30-50 μg/kg. Cardiac function was supported and hemodynamic stability was maintained with vasoactive and inotropic drugs. Measures were taken to strengthen blood conservation and respiratory function support.Seven patients(2.9%) needed urgent CPB during operation because of serious cyanotic spells. Perfusion-induced lung injury occurred in 18 patients (7.6%). Circulation was assisted by ECMO in 2 patients (0.9%). Three patients (1.3%) died of serious low cardiac output and perfusion-induced lung injury.

Objective:To evaluate the influence of the implant-supported porcelain bridges made from non-precious metals using spark erosion techniques, and to discuss the feasibility and details of making the implant restoration by spark erosion technique.Methods: The study included 12 patients ( 9 males and 3 females) with 92 units implant-supported non-precious porcelain bridge from Sep.2011 to Feb. 2013.All the patients received implant treatment in Department of Oral Implantology, Peking University School and Hospital of Stomatology.The total of 52 implants, were from Nobel Biocare implant system, Camlog implant system and Ankylos implant system.The implant analogs were connected in sequence with a copper wire to guarantee conductivity.The implant electrodes represented one electrode and the superstructure the other.During spark-erosion machining, the cast holding the implant electrodes and the prosthetic framework were moved toward one another, causing an electrical erosion of the protruding ele-ments.Results:After the spark-erosion machining, the minimum gap between the framework and abut-ment was 0.21 mm, which was two units bridge.The maximum was 2.59 mm, which was 11 units bridge with 6 implants.The average gap was 0.68 mm.After the spark-erosion machining, the bridge fitted well with the passive position stability.Conclusion: The method of making implant-supported non-precious porcelain bridge reduces costs on patients.Spark erosion has the potential to provide implant framework with an excellent fit.The patients are satisfied with the clinical results.

OBJECTIVE: To observe the effects of Feiji Formula, a compound traditional Chinese herbal medicine, on lung cancer metastasis in mice. METHODS: The lung cancer metastasis model of mice was established in this experiment study. Twenty-four mice were randomly divided into three groups: untreated group, cisplatin group and Feiji Formula group. Mice in the Feiji Formula group were treated with Feiji Formula decoction; in cisplatin group, with cisplatin by intraperitoneal injection; and in the untreated group, with normal saline (NS). After twenty-day treatment, the body and tumor weights as well as the number of metastatic tumors in both lungs of each mouse were measured. RESULTS: The body weight of mice in cisplatin group was significantly less than that of Feiji Formula group and untreated group (P<0.01); the tumor weight of mice in cisplatin group and Feiji Formula group was markedly lower than that of untreated group (P<0.01); and the number of metastatic tumors in cisplatin group and Feiji Formula group was markedly lower than that of the untreated group (P<0.01), no significant difference between the Feiji Formula group and cisplatin group in terms of the weights and the numbers of metastatic tumors in bilateral lungs. CONCLUSION: Feiji Formula can suppress tumor growth and decrease the number of lung metastatic tumors in the mice, and maintain the body weight of the mice.