Objective To study the surgical treatment and the way of incision of breast fibroma.Methods 106 patients with breast tumor were selected as the research subjects,and they were randomly divided into two groups.53 cases in the conventional incision group used the traditional radial incision surgery.53 cases in the ring areola incision group received the areola incision surgery.The resection time,intraoperative bleeding,postoperative hospital stay and wound healing rate,bilateral symmetry rate,patient satisfaction and postoperative complications were compared between the two groups.Results The incision healing rate,bilateral breast symmetry and the patients' satisfaction rate of the areola incision group were 100.00％,94.34％,94.34％,respectively,which were significantly higher than those of the traditional incision group (81.13 ％,66.04％,81.13 ％),the differences were statistically significant (x2 =5.341,10.541,4.296,all P ＜ 0.05).The incidence rate of postoperative complication of the areola incision group was 1.89％,which was significantly lower than 15.09％ of the conventional incision group (x2 =5.949,P ＜ 0.05).The breast fibroma resection time,intraoperative bleeding volume,postoperative hospitalization time of the areola incision group were (15.14 ± 0.15) min,(20.02 ± 1.53) mL,(5.52 ± 1.12) d,which were significantly better than those of the conventional incision group [(27.24 ± 1.91) min,(29.98 ± 2.72) mL,(7.26 ± 2.57) d],the differences were statistically significant (t =11.113,8.924,8.255,all P ＜ 0.05).Conclusion The surgical treatment of breast fibroma and incision can affect the treatment effect,the areola incision surgery is better than the traditional radial incision,it can shorten operation time,reduce bleeding,reduce the incidence of complications,improve the healing rate,double side breast symmetry rate,the degree of satisfaction of patients,shorten the hospitalization time,which is worthy of promotion.

In our experiment, strain guage was used to investigate the stress distribution in half-ring sulcated external fixator on fresh human tibia and the influence of the number and diameter of stainless steel pin and the compression at fracture ends on stress distribution. The conclusions are as follows: half-ring Sulcated external fixator has no significant influence on the stress distribution on intact human tibia, the rate of stress protection caused by external skeletal fixation is very small (?=1.486) and half-ring sulcated external fixation can be used to result in equal compression at fracture ends; increasing the number and diameter of pin will significantly increase the rate of stress-protection; compression external fixation can not only improve the stiffness of external fixation, but also reduce the rate of stress protection significantly.

Objective To establish a new enzymatic method for the determination of serum conjugated bilirubin (CB) by the use of bilirubin oxidase (BOD). Method Only the CB was oxidized by BOD under the conditions of pH 5.5 in the presence of sodium fluoride NaF and N-acetylcysteine (NAC) which could prevent the unconjugated and delta bilirubin oxidized. CB was quantitatively determined from a decrease in the absorbance at 450 nm caused by CB oxidization. Results The intra-assay CVs were 6.52% and 0.33% (n=20) at CB concentrations of 17.65 and 301.49 ?mol/L, respectively. The inter-assay CVs were 9.90% and 2.72% at 31.50 and 184.12 ?mol/L, respectively. The linearity was up to 320 ?mol /L. The optimum concentrations of selective inhibitors were 2.5 mmol/L for NaF and 2.5 mmol/L for NAC. The results of serum CB (Y) determined by this method correlated well with those determined by diazo-dye method (Y=0.839X-7.965, r=0.969). Conclusion The proposed reaction conditions could effectively inhibit the oxidation of unconjugated bilirubin by BOD, and thus increase the specificity of CB assay.

The pricking wound which may lead to serious hurt and undesired consequences always happensduring the blood sampling course,but unfortunately it hasn't drawn enough attention from the public.China is aprevalent area for hepatitis,and what is more,AIDS also shows an increasing tendency in recent years.Bloodpollution by the pricking wound accounts for a major occupational pollution and infection reason for thehematogenously infectious diseases.Based on his practical work experience and real data,the author expounds thecauses and precautions of the pricking wounds and puts forward some relevant policies and suggestions as well.

Objective To rapidly construct hepatitis B virus(HBV)adefovir(ADV)‐resistant strain(RT A181T)infectious clone by using SOE‐PCR ,to observe the expression of recombinant plasmids in Huh7 cells and to establish the in vitro cell model of HBV ADV‐resistant strain(RT A181T) .Methods The conservative primers were designed ,the full‐length HBV genome was amplified from serum in the patients with ADV‐resistant chronic hepatitis B .Then ,the 1 .3‐fold genome‐length infectious clone pHBV1 .3 was constructed by using the SOE‐PCR technique ,which was transfected into human hepatoma cells Huh7 cell line .ELISA ,Western Blot and Real‐time PCR were adopted to detect infectious cloning replication and expression ,meanwhile the antiviral drugs lamivu‐dine(LAM ) and ADV were used to verify the infectious clone copy and inhibition of expression .Results The HBV ADV‐resistant infectious cloning plasmids pHBV1 .3 was successfully constructed .This plasmid could be effectively replicated ,transcripted and ex‐pressed in Huh7 cell lines .LAM could effectively inhibit the replication and expression of the infectious clone ,while ADV could not inhibit the replication and expression of the infectious clone .Conclusion The constructed infectious clone plasmids pHBV1 .3(RT A181T ) of HBV ADV‐resistant strain can efficiently replicate and express protein in vitro ,its transfected cells can be used in the study of HBV replication mechanism and antiviral study .

Objective To investigate the effects of pulsed electromagnetic fields(PEMF)on the bone mineral density(BMD)and serum level of transforming growth factor-beta 1(TGF-?1)in the disuse osteoporosis(DOP)rats.Methods Eighty 4-month-old female SD rats were randomly divided into 4 matched groups according to their weight:control group(INT group),DOP group,calcitonin group(CT group)and PEMF group.Rats in INT group were raised with the average amount of food consumed by the DOP rats.Right hindlimbs of rats in DOP,CT and PEMF groups were immobilized by tibia-tail fixation to establish DOP model as Zarzhevsky described.Rats in CT group were injected with calcitonin(2 IU/kg,i.p,once a day),and rats in group PEMF were irradiated with PEMF(8 Gs,15 Hz,a pulse time of 8 ms)immediately after operation.The rats(5 animals per time of each group)were killed at 1,2,4 and 8 weeks after operation and their right hindlimbs were resected.The BMD and serum TGF-?1 concentration were measured with a dual energy X-ray bone densitometer and ELISA respectively.Results Significant difference was observed in the femur proximal BMD between rats with right hindlimb immobilization(DOP group)and INT group 2 weeks later(P

Objective To study the effects of strontium (Sr) on proliferation and osteogenic differentiation of human marrow mesenchymal stem cells (hMSCs) in vitro,and to determine its optimal concentration.Methods Six milliliter of bone marrow was obtained from a healthy adult volunteer,the hMSCs were then isolated and cultured in vitro,and the cell phenotype was identified by flow cytometry.The 4th passage cells were used for experiment,and they were randomly divided into 5 groups:the osteogenic agents (10-8mol/L dexamethasone,50?g/ml ascorbic acid and 10mmol/L beta-sodium glycerophosphate) were used alone in the control group (group A),and the combination of osteogenic agents and different doses of strontium chloride (3.75?10-3,3.75?10-2,3.75?10-1 and 3.75mmol/L) were used in group B,C,D and E.The proliferation level,alkaline phosphatase (ALP) activity and osteocalcin (OCN) content of MSCs were determined by MTT colorimetric assay,enzyme-labeled method and radioimmunological method,respectively.The amount and area of calcium nodules in each group were observed by alizarin red staining.Results The proliferation and osteogenic differentiation rate of each group increased significantly with the lapse of culture time(P

To systematically evaluate the importance of protein synthesis in ischemic preconditioning (PC)-induced ischemic tolerance (IT), temporary middle cerebral artery occlusion (MCAO) by Longa (20 min) was used for PC (ischemic precondioning). Twenty-four hours of reperfusion was allowed after PC and before permanent MCAO to establish ischemic tolerance (IT) to compare with non-PC (sham-operated) rats (n = 5 for each group). Infarct size and neurological deficits were measured 24 h after PMCAO. Samples of brain were taken for the determination of HSP70 expression by Western blot analysis. The effects of the protein synthesis inhibitor cycloheximide administered just before PC or administered long after PC but just before PMCAO on IT were also determined (n = 5 for each group). Our results showed that hemispheric infarct was significantly reduced (P < 0.01) only if PC was performed after 24 h, and PC significantly (P < 0.05) reduced neurological deficits (similar to reductions in infarct size). Cycloheximide eliminated ischemic PC-induced IT effects on bothbrain injury and neurological deficits if administered before PC but not if administered long after PC but before PMCAO. PC produced no brain injury but did increase HSP70 protein 24 h after PC. Cycloheximide eliminated that effect. The results suggest that PC is a powerful inducer of ischemic brain tolerance as reflected by the preservation of brain tissue and motor function. PC induces IT that is dependent on de novo protein synthesis.

Objective To investigate the features of executive functions(EFs) of children with Tourette's syndrome(TS).Methods Several EFs such as working memory,attention and inhibition,flexibility,planning and diversion were measured in TS group(53 child patients) and control group(51 healthy children) with the Visual Figure Memory Tests,Fluency Tests,Stroop Tests(C and CW),Wisconsin Card Sorting Test(WCST).Results Compared to control group,the patients with TS had significant poor executive performances in Visual Figure Memory Tests,Fluency Tests,Stroop Tests and WCST(all P

Objective:To investigate the role of glucose-regulated protein 78(GRP78) and caspase-9 and-12 in intrauterine distress-induced hypoxic-ischemic cerebral damage in fetal rats.Methods:The fetal rat model of intrauterine distress was constructed and the fetal rats were randomly divided into a normal,a sham operation and an ischemia-reperfusion(IR) group.Neuron apoptosis was analyzed by in situ end-labeled DNA(TUNEL).The expressions of caspase-9 and-12 and GRP78 proteins in the hippocampus CA1 area were detected by immunohistochemical staining.Results:Ischemia-reperfusion damage induced classic neuron apoptosis and the number of the apoptotic neurons in the hippocampus CA1 area increased with the progression of reperfusion.The expressions of GRP78 and caspase-9 and-12 were weak in the normal and sham operation group.In the IR group,the expression of GRP78 reached the peak value 3 hours after the reperfusion and then decreased gradually;the intensity of caspase-12 was increased rapidly while that of caspase-9 elevated very little within 3 hours,but both reached the peak value at 12 hours.Conclusion:Intrauterine distress-induced hypoxic-ischemic cerebral damage in fetal rats may trigger the homeostatic control system in the endoplasmic reticulum through the increased expression of GRP78.The apoptotic pathway mediated by caspase-12 in the endoplasmic reticulum may be one of the mechanisms underlying cerebral ischemic injury.