Objective To investigate the dose response relationship for K562 cells' loss of proliferation ability induced by 60Co γ ray irradiation. K562 cell morphological change and Egr-1 expression level are also investigated. Methods 3H-TdR incoporation assay, Giemsa Staining, RT-PCR were used in this study.Results It was noted that the proliferation inhibition is positively dose-dependent. K562 cells showed extensive changes in both morphology and Giemsa staining characteristics after radiation exposure. The irradiated cells acquired characteristics which the high differentiated cells had only. In the early stage after exposure to 5 Gy 60Co γ ray irradiation. A transient transcriptional upregulation of Egr-1 gene which was related to apoptosis and differentiation was observed.The transcriptional level reached its top on time point of 0.5 ～1 hours after exposure. Eight hours after exposure, the transcriptional level was below normal.Conclusions The proliferation inhibition of K562 cell is positively dose dependent. Egr-1 gene can be rapidly activated after exposure to 5 Gy 60Coγ ray. After exposure to 60Co γ ray irradiation, K562 cells entered into a more mature stage on differentiation of morphological

Objective:To explore the effect of diagnosis and treatment in corneal pregnancy by hysteroscope under ultrasound mediated.Methods: Twelve corneal pregnancy wemen as retrospective analysis were collected and observed the gestational sac location, shape, size, echo, blood flow distribution and whether the consolidated common postoperative complications. Results: Twelve patients under ultrasound monitoring by hysteroscopy positioning, removal of lesions one-time success in 11 cases. The residual of palace angle, 1 case of hysteroscopy prompt receives review of the palace using ring of hysteroscopic resection lesions. This group of patients was cured.Conclusion: Orientation of ultrasound mediated by hysteroscopy diagnosis and curettage surgery, is an effective method of diagnosis and treatment of pregnancy of palace angle, judgment cornual pregnancy in high accuracy, preserve fertility in patients, can be repeated.

Objective To explore factors affecting diagnosis and treatment compliance in patients with gout leaving hospital,in order to provide a reference for late-stage clinical treatment.Methods 390 patients with gout treated in our hospital were selected from August 2012 to February 2016 for the study,all patients were followed up by telephone,their diagnosis and treatment compliance after discharge was investigated,and the factors affecting diagnosis and treatment compliance in gout patients after discharge investigated were,as well.Results The mean score of treatment adherence of 390 cases of gout patients after discharge was(3.93± 1.78) points.There were 117(30.00％) patients of which their score were ≥6 points for compliers,and 273 (70.00％) patients who do not comply with scores ＜6 points.Disease duration,education level,frequency of gout attacks,marital status and payment of medical expenses were closely related to diagnosis and treatment adherence(P＜0.05).Longer duration,low educational level,payment of medical expenses at their own expense were independent risk factors for poor adherence (P＜0.05).Conclusion We should strengthen the monitoring and follow-up of patients with gout who are with long duration of disease,low educational level,and pay medical expenses from their own pocke tinorder to improve patient diagnosis and treatment adherence,provide references for late-stage clinical treatment.

OBJECTIVE:To introduce the preparation of Rosa roxburghii tratt's SOD oral liquid and its therapeutic effect on insomnia.METHODS:To mix Rosa roxburghii tratt's juice with Rhizoma Aractyloidis's decoction in proper proportion.To control the quality by detecting activity of SOD .The therapeutic effect on insomnia was observed in 34 patients .RESULTS:The quality was not changed obviously after storage for 18 months;The effective rate for insomnia was 88.2%. CONCLUSION:The preparation is safe,stable and effective.

Objective To explore the effect of L-carnitine on panel reactive antibody (PRA) in hemodialysis patients. Methods 50 patients were classified randomly into 2 groups: L-carnitine group receiving intravenous injection of 2g L-carnitine after each hemodialysis for 6 months, and control group did not receive any L-carnitine treatment. The PRA in serum was measured by enzyme-linked immunosorbent assay (ELISA) before and after 6 months of L-carnitine treatment. Results L-carnitine significantly reduced PRA levels compared with control group(P

AIM: To observe the curative effect of reconstruction of extensor tendon by transplanting heterologous tendon in the treatment of mallet finger deformity. METHODS: Fifteen cases of mallet finger deformity treated by reconstruction insertion of extensor tendon were enrolled at Department of Orthopedics, Xuzhou Hospital Affiliated to Medical College, Southeast University from January 2003 to March 2006. They all participated the observation voluntarily. Two open injury patients were treated with operation in the first emergency phase. The 13 closed injury patients were treated with operation in the first week phase. ①operation method: After brachial plexus anesthesia, an incision was conducted on the back of distal interdigital joint. A bone tunnel was made at 4 mm distance from the digital interphalangeal joint and deviation to the back of distal phalanx. A suit heterologous tendon was passed through the bone tunnel and was sutured with the lateral tendon after crossing on the back of the digital interphalangeal joint (the distal interphalangeal joint in 10?-15? extension by 1.0 mm diameter keith needle). Fixation with plaster splint, function practice of the distal phalanx could be performed when the plaster splint and the keith needle were removed after 6 weeks. Follow-up was done regularly after operation. ②function assessment: The total extension lag angle of metacarpophalangeal joint, proximal articulations interphalangeae, distal articulations interphalangeae at maximal extension position and the distance between finger tip and transverse striation at finger flexion position were measured. 0?indicated excellent flexion and finger tip was over transverse striation at finger flexion; at most -15? indicated good flexion and finger tip touched transverse striation at finger flexion. RESULTS: Totally 15 patients were involved in the result analysis, no drop-out. Postoperative follow up ranged from 2 months to 3 years, with an average of 19.5 months. The wound of all patients was good and no reject reaction. According to Dargan criteria, the overall effective rate was 93.3%. Excellent result was obtained in 12 cases (80%) and good in 2 cases (13.3%). One case was distal interphalangeal joint stiffness and flexion function was not free. CONCLUSION: Reconstruction of extensor tendon is an effective method for mallet finger deformity.

Objective To evaluate the therapeutic efficacy of congenital muscular torticollis (CMT) by two different methods. Methods A total of 86 patients with congenital muscular torticollis in recent 7 years were involved in this study, including 69 cases under 12 years old and 17 cases over 12. Two different operation methods, incision of inferior extremity of sternocleidomastoid muscle and incision of amphi-sternocleidomastoid muscle with post-operative traction, were alternatively chosen according to the ages and deformed degrees of the patients. Results 72 patients were followed-up for 1 to 7 years (averaged 4.5 years). Based on the effect of function and cosmetology in the group of 57 cases under 12 years old, there were 52 cases (91.2 %) excellent, 5 cases (8.8 %) good and no case with side-effects. Meanwhile, in the group of 15 cases over 12 years old, there were 11 cases (73.3 %) excellent, 4 cases (26.7 %) good and no case with side-effects. The effect was satisfactory. Conclusion Different patients with congenital muscular torticollis should be treated with different therapeutic methods.

Objective To study the relationship between the plasma levels of C-reactive protein (CRP) and the severity of disease as well as outcome in patients with cerebral hemorrhage at early stage. Methods The CRP plasma levels were measured by scatter turbidimetry in 89 patients with cerebral hemorrhage within 24 hrs after onset of the disease. The control group included 30 healthy people who received medical examinations. We analyzed the relationship between CRP levels and the severity of disease as well as outcome.Results CRP levels were less than 8 mg/L (4.21?1.78 mg/L) in control group, and increased in 51 patients with cerebral hemorrhage (30.33?2.53 mg/L), with an abnormal rate of 57.30%. CRP levels and abnormal rate differed significantly (all P

Objective:To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer. Methods:A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results:PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups. Conclusion:Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.

Objective To study clinical results of percutaneous vertebroplasty(PVP) for the treatment of symptomatic vertebral hemangiomas.Methods A total of 10 patients with vertebral hemangioma (thoracic vertebrae,6 patients;lumbar vertebrae,4 patients) were treated from December 2002 to April 2005.Under the guidance of digital subtraction angiography(DSA),a needle was advanced into the vertebral body via a unipedicular or bipedicular approach.The preparation of polymethylmethacrylate(PMMA) was mixed with 3 doses of powder,2 doses of liquid,and 1 dose of contrast until a doughy,cohesive consistency similar to toothpaste was obtained.Then the PMMA cement was injected into the vertebral body by using a high-pressure plunger.The total injection volume ranged 4~6 ml(mean,4.8 ml) in the thoracic vertebrae and 5~8 ml(mean,6.0 ml) in the lumbar vertebrae.Results The procedure was completed smoothly in all 10 patients.The operation time was 25~60 min(mean,39.5 min) and the intraoperative blood loss was 10~50 ml(mean,19.6 ml).No incidence of cement leakage or pulmonary embolism occurred.The 10 patients were followed for 2~30 months(mean,18 months).In 9 patients,free of signs and symptoms was achieved and no vertebral compression fracture or recurrence of hemangioma was observed.One patient with L_3 hemangioma still complained of mild and paroxysmal loin pain at 1 year after procedure,but the patient's normal activities were unaffected and no nervous dysfunction was found.Conclusions Percutaneous vertebroplasty is a safe and effective treatment for symptomatic vertebral hemangiomas.