Objective To explore factors affecting diagnosis and treatment compliance in patients with gout leaving hospital,in order to provide a reference for late-stage clinical treatment.Methods 390 patients with gout treated in our hospital were selected from August 2012 to February 2016 for the study,all patients were followed up by telephone,their diagnosis and treatment compliance after discharge was investigated,and the factors affecting diagnosis and treatment compliance in gout patients after discharge investigated were,as well.Results The mean score of treatment adherence of 390 cases of gout patients after discharge was(3.93± 1.78) points.There were 117(30.00％) patients of which their score were ≥6 points for compliers,and 273 (70.00％) patients who do not comply with scores ＜6 points.Disease duration,education level,frequency of gout attacks,marital status and payment of medical expenses were closely related to diagnosis and treatment adherence(P＜0.05).Longer duration,low educational level,payment of medical expenses at their own expense were independent risk factors for poor adherence (P＜0.05).Conclusion We should strengthen the monitoring and follow-up of patients with gout who are with long duration of disease,low educational level,and pay medical expenses from their own pocke tinorder to improve patient diagnosis and treatment adherence,provide references for late-stage clinical treatment.

Objective:To explore the effect of diagnosis and treatment in corneal pregnancy by hysteroscope under ultrasound mediated.Methods: Twelve corneal pregnancy wemen as retrospective analysis were collected and observed the gestational sac location, shape, size, echo, blood flow distribution and whether the consolidated common postoperative complications. Results: Twelve patients under ultrasound monitoring by hysteroscopy positioning, removal of lesions one-time success in 11 cases. The residual of palace angle, 1 case of hysteroscopy prompt receives review of the palace using ring of hysteroscopic resection lesions. This group of patients was cured.Conclusion: Orientation of ultrasound mediated by hysteroscopy diagnosis and curettage surgery, is an effective method of diagnosis and treatment of pregnancy of palace angle, judgment cornual pregnancy in high accuracy, preserve fertility in patients, can be repeated.

Objective To investigate the dose response relationship for K562 cells' loss of proliferation ability induced by 60Co γ ray irradiation. K562 cell morphological change and Egr-1 expression level are also investigated. Methods 3H-TdR incoporation assay, Giemsa Staining, RT-PCR were used in this study.Results It was noted that the proliferation inhibition is positively dose-dependent. K562 cells showed extensive changes in both morphology and Giemsa staining characteristics after radiation exposure. The irradiated cells acquired characteristics which the high differentiated cells had only. In the early stage after exposure to 5 Gy 60Co γ ray irradiation. A transient transcriptional upregulation of Egr-1 gene which was related to apoptosis and differentiation was observed.The transcriptional level reached its top on time point of 0.5 ～1 hours after exposure. Eight hours after exposure, the transcriptional level was below normal.Conclusions The proliferation inhibition of K562 cell is positively dose dependent. Egr-1 gene can be rapidly activated after exposure to 5 Gy 60Coγ ray. After exposure to 60Co γ ray irradiation, K562 cells entered into a more mature stage on differentiation of morphological

OBJECTIVE:To introduce the preparation of Rosa roxburghii tratt's SOD oral liquid and its therapeutic effect on insomnia.METHODS:To mix Rosa roxburghii tratt's juice with Rhizoma Aractyloidis's decoction in proper proportion.To control the quality by detecting activity of SOD .The therapeutic effect on insomnia was observed in 34 patients .RESULTS:The quality was not changed obviously after storage for 18 months;The effective rate for insomnia was 88.2%. CONCLUSION:The preparation is safe,stable and effective.

Objective:To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer. Methods:A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results:PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups. Conclusion:Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.

Objective To observe the effect of self-assembling Atractylodes Rhizome Decoction combined with quadruple therapy on Helicobacter pylori (Hp)-positive peptic ulcer and its effect on wound healing, gastric acid secretion and recurrence.MethodsThis study was based on 70 patients with Hp-positive peptic ulcer treated in our hospital from March 2012 to May 2014.The patients were divided into study group and control group according to the order of admission.(BAO), the maximum gastric acid secretion (MAO), the peak gastric acid secretion (P<0.05).The treatment group is divided into two groups according to the treatment group.(PAO), Hp eradication and recurrence were observed and compared.ResultsAfter treatment, the total effective rate of wound healing study group was 80.00% higher than that 57.14% of the control group (χ2=4.242, P<0.05).The levels of BAO, MAO and PAO in the two groups were significantly lower than those before treatment (P<0.05).The eradication rates of Hp in the study group were significantly higher than those in the control group at 6 months and 1 year follow-up.The recurrence rate was significantly lower in the study group than in the control group (P<0.05),There was no significant difference between the two groups.ConclusionThe clinical effect of self-made Baishui Yipi Decoction combined with quadruple therapy on Hp-positive peptic ulcer patients is effective in promoting wound healing and Hp eradication, and inhibiting gastric acid secretion and reducing the recurrence rate.It is a kind of medicine worthy of clinical application Program.

The expressions of matrix metalloproteinnse-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) ,and NF-κB in 157 cases of papillary thyroid cancer and 30 cases of papillary thyroid tumors were detected by immunohistochemistry and in situ hybridization. The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers were significantly higher than those in benign tumors (all P <0.01), and increased according to the pathological grading (P<0.01). The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers with cervical lymph node metastasis were higher than those without metastasis (both P <0.01). The expressions of TIMP-1 protein and mRNA appeared an opposite direction of MMP-9 expression.

Objective To study the relationships between hemoglobin concentration,cytokine and cardiac function in patients with ischemic cardiomyopathy.Methods Hemoglobin concentration,sernm TNF-α,IL-6 in 121 patients with ischemic cardiomyopathy were measured,left ventricular ejection fraction(LVEF)by echocardiography,according to hemoglobin concentration,these patients were distributed to the anemic group and the non-anemic group,contrasting to 27 healthy persons.Results The levels of serum TNF-α,IL-6 in the anemic patients group were higher than those in the non-anemic group subjects,but LVEF Wag lower.With the increase of anemia severity,the levels of serum TNF-α,IL-6 significantly increased;there was a positive correlation between LVEF and the levels of serum TNF-α,IL-6(P＜0.01),and a negative correlation between LVEF and Hb concentration.Conclusion The levels of cytokine promote the pathophysiological procedure between cardiac failure and anemic in ICM.

Objective To study whether a lactose-free,hydrolyzed protein formula would ameliorate feeding intolerance and weight gain in very low birth weight infants.Methods The study was conducted on very low birth weight infants ( gestational age ≤34 weeks, birth weight ≤1500 g) in our NICU from August 2010 to August 2013. According to the number of admission, the cases were assigned into lactose-free hydrolyzed protein formula group ( hydrolyzed protein group) and preterm formula group. The data of basic information ( gestational age, birth weight, way of labor, sex, asphyxia ), commencement of feeding time ( days) , enteral caloric intake, parenteral nutrition caloric intake, The time ( days) required to regain birth weight, weight gain index, days of parenteral nutrition, prealbumin level, feeding intolerance, the incidence of necrotizing enterocolitis ( NEC ) and extrauterine growth retardation(EUGR)werecollected.Results A total of 83 infants were recruited in the study. 40 infants were in hydrolyzed protein group and 43 infants were in preterm formula group. There were no differences between groups for basic information ( P>0. 05). The weight gain index of hydrolyzed protein group was higher than that of preterm formula group (13. 5 ± 2. 4 g/d vs. 11. 9 ± 3. 7 g/d, P=0. 036). There were less cases of feeding intolerance in hydrolyzed protein group than in preterm formula group & nbsp;(28/40 vs. 38/43, P=0. 038). The differences of the incidence of NEC (1/40 vs. 2/43) and EUGR (21/40vs.25/43)showed no significance statistically.Conclusions Lactose-free hydrolyzed protein formula can obviously improve feeding intolerance and early weight gain of premature infants.

Objective To study clinical results of percutaneous vertebroplasty(PVP) for the treatment of symptomatic vertebral hemangiomas.Methods A total of 10 patients with vertebral hemangioma (thoracic vertebrae,6 patients;lumbar vertebrae,4 patients) were treated from December 2002 to April 2005.Under the guidance of digital subtraction angiography(DSA),a needle was advanced into the vertebral body via a unipedicular or bipedicular approach.The preparation of polymethylmethacrylate(PMMA) was mixed with 3 doses of powder,2 doses of liquid,and 1 dose of contrast until a doughy,cohesive consistency similar to toothpaste was obtained.Then the PMMA cement was injected into the vertebral body by using a high-pressure plunger.The total injection volume ranged 4~6 ml(mean,4.8 ml) in the thoracic vertebrae and 5~8 ml(mean,6.0 ml) in the lumbar vertebrae.Results The procedure was completed smoothly in all 10 patients.The operation time was 25~60 min(mean,39.5 min) and the intraoperative blood loss was 10~50 ml(mean,19.6 ml).No incidence of cement leakage or pulmonary embolism occurred.The 10 patients were followed for 2~30 months(mean,18 months).In 9 patients,free of signs and symptoms was achieved and no vertebral compression fracture or recurrence of hemangioma was observed.One patient with L_3 hemangioma still complained of mild and paroxysmal loin pain at 1 year after procedure,but the patient's normal activities were unaffected and no nervous dysfunction was found.Conclusions Percutaneous vertebroplasty is a safe and effective treatment for symptomatic vertebral hemangiomas.