In the supporting electrolyte of 0.2mol/L LiCl, a distinct cathodic wave was produced by using first or seeondary derivative oscillopolarography to measure maleic hydrazide.The peak potential was -1.95V (Vs.SCE).The peak current was linearly proportional to the concentration of maleic hydrazide in the range of 1.0?10~(-4)mol/L to 2.0?10~(-5)mol/L and 1.5?10~(-5)mol/L to 4.0?10~(-6)mol/L.(PH5.6～9.3).The detection limit was lower than 2.0 ?10~(-6)mol/L.The error of various methods(relative deviation)was less than 0.3 per cent. Therefore,a simple,rapid and accurate analytical method for determining the purity of maleic hydrazide has been afforded in this paper.

Objective To study the compound oral contraceptives(COC)application in patients with missed abortion after.Methods 600 cases of missed abortion from March 2016～2017 year in March in our hospital,were randomly divided into observation group(n=300)and control group(n=300),the observation group was treated with compound oral contraceptive therapy,the control group were given routine treatment,compared two groups of patients.Results The observation group of patients with vaginal bleeding time,menstrual recovery time was significantly shorter than the control group(P<0.05),endometrial thickness was significantly greater than the control group(P<0.05),the incidence of Intrauterine Residue and uterine cavity adhesion rate,pelvic infection rate was significantly lower than the control group(P<0.05); the two groups of patients with postoperative weight change in March the amount of vaginal bleeding,compared no significant difference,no statistical significance.Conclusion The application of compound oral contraceptives in the treatment of missed abortion patients after curettage,shorten the time of vaginal bleeding,reduce the amount of vaginal bleeding,reduce the incidence of postoperative complications and promote postoperative recovery of menses.

Objective To study the relationship of Hp infection and gastric juice vitamin C concentration to gastric mucosa lesions,as well as its mechanisms of pathogenesis and carcinogenesis.Methods Vitamin C concentration in gastric juice was measured by using reduced ferric method.Hp was determined by fast urease test,pathologic Giemsa stain and bacterial culture.Hp eradication treatment was performed on part of Hp positive patients,and the vitamin C concentration in gastric juice before and after Hp eradication was compared. Results Gastric juice vitamin C concentrations in each Hp positive test groups were:(18.3±4.5)mg/L(chronic superficial gastritis group),(9.1±3.3)mg/L(atrophic gastritis with intestinal metaplasia group),(8.2±2.7)mg/L(dysplasia group),and (5.2±1.6)mg/L(gastric cancer group) respectively,which were significantly lower than that in control group [(35.2±7.2)mg/L],P＜0.05.After eradication of Hp,the vitamin C concentrations in gastric juice returned to normal,whereas it had no significant difference in those with failure of Hp eradication.Conclusions Hp infection may cause a significant lowering of gastric juice vitamin C concentration,which may be one of the mechanisms of its pathogenesis and carcinogenesis.Eradication of Hp can return the vitamin C concentration in gastric juice to normal,which may contribute to guard against the occurrence of gastric cancer.

Objective To investigate the applicative value of enhanced 3D multi-echo GRE T2*-weighted angiography(ESWAN) sequence phase values in evaluating brain gray nuclei iron content in idiopathic restless legs syndrome(RLS) patients, providing imaging basis in diagnosis and treatment of idiopathic RLS. Methods In our institute from June 2012 to September 2016,forty-five RLS patients were selected as the RLS group, and 45 healthy volunteers as the control group. ESWAN sequence was performed and serum ferritin values were obtained in all patients and volunteers. The raw data of ESWAN was postprocessed , where the phase maps were obtained. Phase analysis was performed on localized brain gray nuclei regions of interest (substantia nigra, red nucleus, dentate nucleus, thalamus, pallidum, putamen and caudate nucleus ) selected on phase maps. Differences between the 2 subject groups were evaluated using ANCOVA including age as a covariate. Results The phase values of the substantia nigra, thalamus, pallidum and putamen in the RLS group were (-0.087 ± 0.021), (-0.053 ± 0.012), (-0.161 ± 0.008), (-0.125 ± 0.019) radians , respectively. The phase values of the substantia nigra, thalamus, pallidum and putamen in the control group were (-0.127 ± 0.007), (-0.066 ± 0.007), (-0.166 ± 0.007), (-0.150 ± 0.010) radians, respectively. There were significant differences between the two groups (F=142.492, 37.988, 10.558, 60.725;P<0.05). Indicating reduced iron content in several regions of brain gray nuclei of the patients with RLS. Serum ferritin concentration between the RLS patients (157.02±95.78)μg/L and healthy controls (175.49 ± 38.65)μg/L was not significant (F=1.353,P>0.05). Conclusions Phase values can make a quantitative assessment of brain gray nuclei iron content in RLS patients, our results supported the hypothesis of reduced brain iron content in RLS patents , which may have an important role in the pathogenesis of the disorder. However, iron content change in some brain regions was not correlated with serum ferritin concentration changes.

Purpose:To evaluate the effect of 3DRT for malignant biliary obstruction and to find a more effective method of treatment.Methods:Form October 1998 to September 2000,35 patients with malignant biliary obstruction ,3-4 Gy of 5 fractions a week to total doses from 38 to 48 Gy.Results:All cases completed radiation. CR rates were 24%,PR rates were 63%,the overal response rate (CR+PR) was 87%. A significant decrease of bilirubin levels was observed in 29 patients,no serious complications occured. 1-2 year survival rates were 70% and 21%.Conclusions:3DRT is well tolerated in most patients with malignant biliary obstruction,its effect is better than the conventional radiation and it is a more effective treatment for the malignant biliary obstruction.

Objective To analyze the polymorphisms of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) gene Exon1 and Exon25,GPI-PLD activity of leucocyte in peripheral blood and their relationship in systemic lupus erythematosus patients of Han nationality in Hunan Chinese.Methods The polymorphisms were analyzed by PCR-SSCP and sequencing. GPI-PLD levels were determined by using glycosylphosphatidylinositol (GPI) anchored placental alkaline phosphatase (PLAP) as substrate and triton-X114 partioning.Results There were fourteen variations in the coverage of GPI-PLD gene Exon 1 of both 109 healthy persons' and 62 systemic lupus erythematosus patients',including seven variations at transcriptional start site upstream and seven variations at Exon1. The codons of variation were 14TTG→AAG (Leu14Lys),17CTC→GTC (Leu17Val),20AGA→AAA (Arg20Lys),21GGT→GGG (Gly21Gly),30GTA→ATA (Val30Ile),which leaded to four missense mutations and one synonymous mutation. In addition, the frequency of the variation at transcriptional start site upstream -24 C→T was significantly different between healthy control and systemic lupus erythematosus patients. At meanwhile, there was a variation in Exon25, the +60496 G→A, between two groups. The total various frequency, which was determined by SSCP, of healthy person was 33 03 percent and systemic lupus erythematosus patient was 25 81 percent. On the basis of the percentage of GPI-anchored PLAP conversion, the leucocyte GPI-PLD activities of total 62 systemic lupus erythematosus patients were measured. As compared with 109 healthy persons as control group, the leukocyte GPI-PLD activities of systemic lupus erythematosus patients were significantly raised, but GPI-PLD activities were not correlated with polymorphism sites significantly.Conclusions The leukocyte GPI-PLD gene in peripheral blood, which belongs to healthy persons and systemic lupus erythematosus patients of Han mationality in Hunan Chinses,is polymorphism.The frequency of transcriptional start site upstream -24 is significantly different between healthy control and systemic lupus erythematosus patients.The leukocyte GPI-PLD activeties of patients' also significantly increase.

Objective To investigate the relationship between the p33 ING1b protein expression and differentiated degree in human gastric carcinoma. Methods p33 ING1b protein expression was detected by S-P immunohistochemical method in gastric carcinoma and normal gastric mucous tissues. Results The positive rates of p33 ING1b protein expression were 75% (15/20) in well-differentiated, 55% (11/20) in moderate-differentiated and 15% (3/20) in poor-differentiated gastric carcinoma, respectively. The positive rate of p33 ING1b protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa (100%, 60/60) (P

Objective To investigate the biotransformation of dihydroartemisinc acid(Ⅰ) by cultured cells of Artemisia annua.Methods Dihydroartemisinic acid was added to the suspension cells of A.annua which had been pre-cultured for 14 d and co-cultured for another 2 d.The biotransfromed products were detected with TLC and HPLC,and isolated by various chromatographic methods.Results The chemical structures of biotransformed products were elucidated on the basis of spectroscopic data.3-?-Hydroxy-dihydroartemisinic acid(Ⅱa) and 3-?-hydroxy-dihydroartemisinic acid(Ⅱb) were obtained after 2 d administration of dihydroartemisinic acid to the suspension cells of A.annua.Ⅱa and Ⅱb yields could reach a maxium mole ratio of 2.6% and 15.7% after 1 d incubation,respectivly.Conclusion It is the first time for the biotransformation of dihydroartemisinic acid to epimeric 3-hydroxyartemisinic acid by using suspension culture cell of A.annua.The results indicate that cells of A.annua have the ability to hydroxylate dihydroartemisinic acid region selectively.

AIM: To investigate the relaxative characteristics of resveratrol(RES) and 17?-Estradiol(EST) on coronary arteries in dogs and its mechanism.METHODS: Rings of canine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution,and then isometric tension was measured.RESULTS: Both RES and EST caused the does-response contractile curve of KCl in K-H solution and CaCl_2 in Ca~(2+)-free K-H solution shift rightward,and their sensitivies and the values of maximum contractions had been decreased.In the N?-L-nitro-arginine,Methylene Blue and Sodium Orthovanadate groups,the vasorelaxant effects of both estrogens were markedly attenuated(P(0.05)).CONCLUSION: Resveratrol and 17?-Estradiol relaxe vascular smooth muscle in an endothelium-dependent manner,and are not related to KATP channel.

Objective To determine the changes of serum glycosylphosphatidylinositol specific phospholipase D(GPI-PLD) activity in patients of several liver disease.Method Glycosylphosphatidylinositol (GPI) anchored placental alkaline phosphatase(PLAP) prepared by ourselves was used as a substrate.After partitioning by triton-X-114,the serum GPI-PLD activity was determined quantitatively and the data was treated by microware of SPSS 10.0.Results On the basis of the percentage of GPI-anchored PLAP conversion,the sera GPI-PLD activities of total 172 patients,included 26 severe acute viral hepatitis as group A,29 liver cirrhosis as group B,32 chronic viral hepatitis as group C,55 mild acute viral hepatitis as group D,30 primary hepatocellular carcinoma as group E,were measured.As compared with 182 healthy presons as control group,the sera GPI-PLD activities of group A and B were significantly reduced;By contraries,the activities of group D and E were significantly raised.The sera GPI-PLD activities of group C compared with healthy control group were not significantly altered.However,when paired Q-test,the changes of serum GPI-PLD activity between all paired groups among this five groups were remarkable.Conclusions The determination of sera GPI-PLD activities in patients can act as a biochemistry index for diagnosis of acute viral hepatitis,liver cirrhosis and primary hepatocellular carcinoma,as well as an auxiliary index for judgment of the curative effect and prognosis of liver diseases.