Objective To explore the improvement of minocycline on negative symptoms in patients with schizophrenia and its influence on tumor necrosis factor (TNF)-α.Methods Patients with schizophrenia were randomly assigned into two groups.One was olanzapine combined with placebo group (control group, 37 cases).The other was olanzapine combined with minocycline group (treatment group, 200 mg ·d-1,po,bid, 38 cases).Both groups were assessed by the positive and negative syndrome scale (PANSS), the scale for the assessment of negative symptoms (SANS), and also tested the level of TNF-α before and after 12 weeks treatments.Results After 12 weeks treatments, the scores of SANS and PANSS in treatment group were significantly lower than those of control group (P<0.05).The level of TNF-α in treatment group[(6.5±1.3) pg ·mL-1]was significantly lower than that of control group[(7.6±1.8) pg ·mL-1] (P<0.01).Conclusion Minocycline can improve negative symptoms in patients with schizophrenia, and the mechanism may be related to lower the level of TNF-α.

Objective To evaluation the Diagnosis value of single and consociation to drawing materials methods with bronchofibroscopy in pulmonary carcinoma.Methods 481 diagnosed patients with pulmonary carcinoma by bronchofibroscopy during July 2000 to July 2006 were analyzed.Four different drawing materials method including clamping,brushing,needling and rinse checking were used to diagnose.The positive rates of these four methods were compared with single and consociation to drawing materials methods.Results The positive rate of clamping,brushing,needling and rinse checking were 83.9%,70%,59.1%and 35.9%respectively,and the total positive rate was 90.2%after being united.Conclusions Bronchofibroscopy is one of the most important methods in diagnosing pulmonary carcinoma. Using multiple drawing materials methods in bronchofibroscopy can improve the accuracy rate of diagnosis of lung cancer significantly.

OBJECTIVE:To analyze the causes of drug quality events took place in our hospital so as to provide reference for the improvement of drug quality.METHODS:Systematic investigations were performed on 150 batches of drugs that had shown quality problems during 2005 and 2006 in our hospital and the possible reasons accountable for the problems were analyzed.RESULTS & CONCLUSIONS:The quality problems were originated from 3 links including production,distribution and use of drugs,and manifested as improper or wrong packaging,labels or package inserts,inappropriate handling,and improper operation of medical staff etc,which worth the highest alert of the concerned departments.

Expression of gamma interferon (IFN-?)-like substance in adrenal pheochromocytoma was investigated with immunohistochemical technique. In 15 out of the 16 cases of adrenal pheochromocytoma positive IFN-@-like immunoreactive substance was detected in the cytoplasm of the tumor cells, whereas in adrenal tissues adjacent to adrenal tumors in 26 cases (pheochromocytoma 12, adrenal corti-col tumors 12, others 2) only two with pheochromocytoma showed positive IFN-?-like immunoreactive substance in the cytoplasm of zona reticularis cells. The results suggest that IFN-? may be a kind of antigen associated with differentiation of cell.

Objective To investigate the effects of the therapy of invigorating the kidney and qi and dissipating blood stasis to removing the toxin on the hepatocyte apoptosis and associated genes in the liver fibrosis rats,and to explore the treatment mechanism of liver fibrosis with TCM.Methods liver fibrosis model rats were induced by CCl4,and the Chinese herbs(Ganlike)with invigorating the kidney and qi and dissipating blood stasis to removing the toxin was given by daily gavage,after the experiment,the pathological changes of liver were observed with optical microscope.Hepatocyte apoptosis was detected with TUNEL assay,apoptosis-associated genes were detected with immunohistochemistry assay.Results The Chinese herbs Ganlike could obvously improve the pathological changes,alleviate the hepatocyte apoptosis and modulate the expression of apoptosis-associated genes.Conclusions The anti-fibrosis mechanism of Ganlike may be to regulate the hepatocyte apoptosis of liver fibrosis rats and to restrain the expressions of hepatocyte Fas/FasL,Bcl-2/Bax.

By analyzing the educational state in Video Science in medical colleges,combining with work practice,it puts forwards the teaching reform thought of it,aiming to jointly exploring and cultivating the clinical medical talents fit to the new TCM,combination of TCM and western medicine in the modern medical Video Science development.

Objective To investigate the indications and mid-term outcomes of periacetabular osteotomy through ilioinguinal approach for developmental dysplasia of the hip (DDH) in middle-aged (40-50years old) adult patients.Methods Data of 10 adult patients who had undergone periacetabular osteotomies through ilioinguinal approach for DDH from August 2005 to February 2010 were retrospectively analyzed.There were 7 females and 3 males,aged from 40 to 47 years (average,42 years).Bilateral hips were involved in 3 patients,and unilateral hip was involved in 7 patients.Before this hospitalization,1 patient had received Chari osteotomy,and 1 had received hip-shelf procedure.According to the T(o)nnis classification,3 hips were classified as Grade 0,5 as Grade Ⅰ and 2 as Grade Ⅱ.The Shenton line was discontinuous in 6 hips.The average lateral center-edge angle was 3.50°±4.88°; the average anterior center-edge angle was 1.80°±5.07°; the average acetabular index angle was 18.20°±3.01°; the average femoral head extrusion index was 31.30％±4.37％; the average Harris hip score was 67.40±7.75.Changes of the indexes mentioned above were observed during the whole period of follow-up.Results All patients were followed up for 10 to 76 months (average,37 months).The T(o)nnis grade improved in 2 cases,from preoperative Grade Ⅰ and Grade Ⅱ to Grade 0 and Grade Ⅰ at final follow-up,respectively.At final follow-up,the Shenton line was discontinuous in 2 hips; the average lateral center-edge angle improved to 30.40°±3.31°,the average anterior center-edge angle improved to 29.50°±3.03°,the average acetabular index angle decreased to 4.50°±2.55°,the average femoral head extrusion index decreased to 9.90％±4.33％; the average Harris hip score increased to 84.10±4.07.The major complications included 3 cases of lateral femoral cutaneous nerve palsy which recovered in 4 to 6 months,and 1 case of iliac fossa hematoma which disappeared in 2 weeks.Conclusion With more strict surgery indication and more meticulous manipulation,periacetabular osteotomy through ilioinguinal approach can achieve satisfactory results in the treatment of DDH in middle-aged adults,which can increase hip joint congruence,relieve hip symptom and delay progression of osteoarthritis.

Objective: Affective disorder patients can be effectively treated by electro-acupuncture methods without many side effects, but the mechanism of the treatment is not known. The object of this study was to discover whether there is change of guanine-nucleotide-binding protein (G-protein) level in platelets membrane from major depression patients before and after treatment by fluoxetine and electro-acupuncture. Methods:We utilized selective antibodies to quantitate the levels of the G-protein ? subunits in platelets membrane of 78 major depression patients (24 received fluoxetine treatment, 24 received electo-acupuncture treatment and 30 received placebo treatment) and 30 matched comparison subjects.Result:Levels of both G?i and G?q of the patient groups were higher than matched normal comparison subjects before treatment of fluoxetine and electro-acupuncture,the level of G?i and G?q did not change after the treatment of fluoxetine, electro-acupuncture and placebo. The level of G?s wasn't significantly different with matched comparison subjects.Conclusion:The levels of G?i、G?q in platelets membrane from major depression patients are higher than normal, the high levels of G?i and G?q in platelets membrane may be a trait abnormality for major depression patients, not a state abnormality for major depression.

AIM: To investigate relevant factors of stem cell transplantation for treating diabetic foot. METHODS: Disposal, outcome, adjuvant method and safety during stem cell transplantation for treatment of diabetic foot were analyzed. RESULTS: ①After autologous stem cells were depurated at a low temperature for 24-48 hours, number of malignant stem cells decreased. Compared to simple stem cell transplantation, tissue regeneration and neovascularization increased after adding fibrin base during transplantation. ②Ankle-brachial index and transcutaneous oxygen pressure were obviously elevated, rest pain and intermittent lameness were improved, new collateral circulation appeared, and arterial blood flow increased in patients undergoing bone marrow stem cell transplantation in the treatment of ischemic disease of lower extremity. ③Chinese medicine for supplement- ing qi and activating blood circulation for stem cell transplantation fully played regulatory effects of biological responses. Adjuvant methods such as ultrasound ablation in combination of stem cell transplantation could significantly increase lower collateral circula- tion of broken popliteal artery. ④No significant adverse reaction or severe outcome was detected in autologous stem cell transplan- tation in a short term. CONCLUSION: ①Autologous stem cells after purified by low temperature can effectively purify malignant stem cells in bone marrow stem cell transplantation. Transplanted outcome can be elevated by adding fibrin. ②Adjuvant methods can enhance outcome of stem cell transplantation. ③Stem cell transplantation for treating diabetic foot has obtained satisfactory outcomes, but some problems still exist.