Objective To investigate the Th1/Th2 cells imbalance in childhood primary nephrotic syndrome(PNS)and its relation to disease activity. Methods Percentage of T-helper-1(Th1) and T-helper-2 (Th2) cells was examined by three-color flow-cytometry in 21 cases of first-onset PNS, 16 cases of remission PNS and 10 healthy children. Results The percentage of Th1, Th2 and Th0 cells in healthy children was (13. 42?4. 36)% , (2. 53 ? 1. 97)% and (1. 25?0. 92)% respectively. In the patients with first-onset PNS, the percentage of Th1, Th2 and Th0 cells was significantly decreased as compared to healthy children [(2. 34 ?2. 09)%, (1.02?0.96)% and (0.40?0.38)% respectively, all P 0. 05]. Because of relatively low percentage of Th1 cells in contrast to Th2 cells in the patients with first-onset PNS, Th1/Th2 ratio was also significantly reduced in those patients, as compared to the patients with remission PNS and the healthy children (2. 43 ? 2.65 vs 4. 17?2. 32 and 5. 41?2. 77, both P

Radix Ginseng and Rhizoma Chuanxiong were identified and the content of ginsenoside Rg_1 was determined by TCL scanning. The results showed that the content of ginsenoside Rg_1 ranged between 0.01770％ and 0.01826％, the recovery rate of ginsenoside Rg_1 was 99.94％ and the coefficient of variation derived from samples was 1.659％. This method is found to be stable, accurate and reliable.

Objective To compare the clinical results of two bundle anterior cruciate ligament(ACL) reconstruction respectively with four and eight strands of hamstring tendons. Methods 76 patients with chronic ACL rupture were randomized separated in two groups for two bundle ACL reconstruction. In the first group the semitendinous tendon was used to make two two-stranded grafts. In the second group the semitendinous tendon was used to make one four-stranded graft, and the gracilis tendon was used to make the other four-stranded graft. 33 patients in the four-strands group and 35 patients in the eight-strands group were followed up for more than one year. IKDC and Lysholm scales were used to evaluate the clinical results. Results In the four-stranded graft group, the side to side difference was

Objective:To study the effect of sensory nerve on fracture of mandible and try to find the possible mechanism.Methods:Thirty-two China rabbits were used and divided into two groups for study.They all suffered from a standardized fracture in the left mandible,and half of them with inferior alveolar nerve section(IANS)or without.The callus were stained to observe the expression of substance P(SP)by immunochemistry and analysed by a computerized-graph analysing system.Results:Immunoreactivites of substance P occurred weakly on Day 7 and 14 after operation,and became stronger at late stage in IANS group.On the contrary,stronger immunoreactivites of substance P occurred at the early stage,less on day 21 posttrauma and stronger again on day 28 in control group.The strongest immunoreactivites at each stage occurred mainly in bone marrow on day 28 posttrauma in both groups.There was significant difference in the expressions of substance P in the callus between the two groups(P

Objective To evaluate the value of the machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging in study of perfusion of normal kidneys of the rabbits.Methods A series of sequent pictures of 16 normal kidneys of 8 rabbits with color Doppler flow imaging(CDFI) and color Doppler power imaging(CDPI) were collected,and time-intensity curves were drawn by the ultrasound instrument after intravenous administration of "Quanfuxian" contrast medium.Quantitative analyses were done by moving the "interest line".Results The time-intensity curves showed as single apex with steep ascending and slowly descending branch after contrast enhancement.Conclusions The machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging could efficiently show the perfusion of kidney.

Objective To introduce the arthroscopic treatment of acute tibial insertion avulsion fracture of posterior cruciate ligament through double posteromedial portal and "Y" shaped bony tunnel, and to evaluate the clinical results. Methods 33 cases of acute tibial insertion avulsion fracture of posterior cruciate ligament were treated arthroscopically through routine portal and double posteromedial portal. The high posteromedial portal was 4 cm proximal to the joint line, and the low posteromedial portal was just at the level of the joint line. The posterior cruciate ligament and avulsion bone fragment were held together with two USP 6 Aesculap polyester threads, which were first wrapped around the posterior cruciate ligament from anterior to posterior, and then tied behind the posterior cruciate ligament and over the bone fragment. The "Y" shaped bone tunnel was made with the common opening at the anteromedial aspect of the tibia and the tunnel arms opened backward at the inferomedial and inferolateral side of the tibial bed respectively. The threads were pulled out through the "Y" shaped bony tunnel and fixed on titanic button. With twisting of the button the threads were tightened and the fixation was insured. The patients were followed up for six months. Fracture reduction and union, knee stability, range of motion, as well as the total knee function were evaluated. Results All fractures were united without displacement. Six months postoperatively, except for one case with Ⅰ degree of positive posterior drawer test, no knee instability was detected; knee extension restored to normal in all cases and average range of flexion were 139??4.1?. 10? to 15? flexion limitation was found in 3 cases. The mean Lysholm knee score was 91.4?3.6. Conclusion Arthroscopic treatment of acute tibial insertion avulsion fracture of posterior cruciate ligament is minimally invasive. The procedure through double posteromedial portal is appropriate, the use of "Y" shaped bony tunnel and button twisting are useful to assure the fixation.

Objective To investigate the relationship between the p33 ING1b protein expression and differentiated degree in human gastric carcinoma. Methods p33 ING1b protein expression was detected by S-P immunohistochemical method in gastric carcinoma and normal gastric mucous tissues. Results The positive rates of p33 ING1b protein expression were 75% (15/20) in well-differentiated, 55% (11/20) in moderate-differentiated and 15% (3/20) in poor-differentiated gastric carcinoma, respectively. The positive rate of p33 ING1b protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa (100%, 60/60) (P

Objective To explore the affection and related factors of risperidone on the metabolic indexes in schizophrenia patients.Methods 56 cases hospitalized schizophrenia patients were treated with risperidone for 16 weeks.Waistline,blood glucose and blood lipid,blood pressure and other indicators were observed before and after treatment,risk factors that caused metabolic syndrome (MS) and abnormal indexes were analyzed,and 36 healthy persons as control group.Results The rate of MS in patient group was obviously higher than that in control group ( 12.50％ vs 0％,P =0.026).Waistline,triglyceride ( TG),high density lipoprotein ( HDL-C ),and 2 hours postprandial blood glucose (2hPG) in patient group ( (4.15 ± 3.14)cm in man and (4.41 ± 2.86)cm in woman,(0.26 ±0.51 ) mmol/L,(-0.09 ±0.19) mmol/L,(0.49 ±0.84) mmol/L) increased compared with that in control group ( ( 0.76 ± 1.09 ) cm in man and ( 0.64 ± 0.81 ) cm in woman,( 0.04 ± 0.15 ) mmol/L,(-0.01 ± 0.12)mmol/L,(0.04 ± 0.35 ) mmol/L) at the 16th weekend,and difference was significantly (P ＜ 0.05 ).Logistic regression analysis showed that the risk factors were family history of diabetes Odds Ratio (OR) 20.34 (95％ CI 1.97 ～210) and age OR 1.19(95％CI 1.03 ～ 1.38),the basis waistline OR 1.26 (95％ CI 1.04 ～ 1.52) and family history of obesity OR 4.27(95％ CI 1.21 ～ 15.1 ),the basis TG OR 73.88 (95％ CI 3.41 ～ 16.01 ) and the basis systolic pressure OR 1.16 (95％ CI 1.00 ～ 1.33 ),the basis waistline OR 1.41 (95 ％ CI 1.08 ～ 1.84 ) and age OR 1.53 (95％ CI 1.02 ～ 1.31 ) in developed MS,abnormal rate of waistline,TG,and 2hPG.Conclusion Risperidone can cause abdominal obesity,metabolic disorders of glucose and lipid even MS.So it shoud be used carefully for the patient who is old and has family history of diabetes and obesity posture.

Objective To introduce the way of arthroscopic patellar retinaculum adjustment and Fulkerson osteotomy, and to study its clinical results. Methods 35 cases of recurrent patella dislocation were treated by modified Fulkerson tibial tubercle anteromedial transfer, and arthroscopic patellar retinaculum adjustment including the medial retinaculum plication and lateral retinaculum releasing. The tibial tubercle was transferred 1.5 cm anteriorly and medially, and fixed with three Kirschner wires. The patients were followed for 25 months in average. The rate of reccurence, the subjective symptoms and the function of the injured leg were evaluated according to the IKDC and Lysholm rating scale. X-ray examination was taken to evaluate the patella-femur congruence. Results There were no recurrence at the last follow-up. Anterior knee pain, though in slight degree, still existed in 8 cases. The IKDC subjective knee scores were 37.4?4.9 and 92.1?5.3(P

The effect of Wei Yao on three kinds of experimental gastric ulcer rat models was reported. It was found that the drug can decrease the ulcer index and reduce the severity of gastric ulcer, and possesses an analgesic effect. The long-term toxicity test of Wei Yao showed that the drug was safe and nontoxic.