ObjectiveTo explore the wall structural ch anges of the carotid with integrated backscatter and their effects on wall distensibility in patients with primary hypertension. MethodsForty-three patients with primary hypertension and 25 normal subjects were enrolled in the study. The corrected integrated backscatter (CIBS) and its cyclic variability (CVIB), the thickness of intima-media (IMT) and the carotid distensibility (CD) were compared between hypertensive group and normal control group. ResultsCIBS and IMT were increased while CD was decreased more significantly in hypertensive patients than in normal subjects. Demonstrated a negative correlation with CD but a positire correlation with IMT in hypertensive patients. ConclusionsThe wall structural changes of the carotid are closely correlated with the functional disorder in patients with primary hypertension.

Objective To investigate the efficacy of acupoint selection at distant places and local tenderness points in treating migraine without aura.Method Sixty patients were randomly allocated, using a random number table, to two groups, 30 cases each. The treatment group received acupuncture at distant points selected along meridians and local tenderness points and the control group, acupuncture at conventionally selected points. The therapeutic effects were evaluated using the visual analogue scale (VAS) and the short-form McGill Pain Questionnaire (SF-MPQ).Result After treatment, the VAS pain score was lower in the treatment group than in the control group; there was a statistically significant difference (P<0.05). The total efficacy rate was 93.3% in the treatment group and 73.3% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Both acupuncture at distant points selected along meridians and local tenderness points and acupuncture at conventionally selected points have a certain therapeutic effect on migraine, but the former produces a better effect than the later and can markedly relieve the pain and improve the quality of life in the patients. The outcome of this study provides a certain reference value for clinical treatment of this disease.

OBJECTIVE:To establish methods for the determination of the metal elements and the Total flavonoids in Flos Lonicerae Japonicae.METHODS:The atomic absorption spectrometry was adopted to determine the contents of macroelements(Na,K,Ca,Mg)and trace elements(Cu,Zn,Fe,Mn,Cr,Ni)while UV spectrometry was adopted for the determination of the content of Total flavonoids in Flos Lonicerae Japonicae.RESULTS:Metal elements and Flavonoids were found to be rich in Flos Lonicerae Japonicae.CONCLUSION:The methods adopted in this study were simple,rapid,accurate,sensitive and precise.

Objective To observe the clinical efficacy of deep needling at Tianshu (ST 25) and Fujie (SP 14) plus electroacupuncture in treating severe functional constipation (FC).Method Sixty-three patients with severe FC were randomized into a treatment group of 33 cases and a control group of 30 cases. The treatment group was intervened by deep needling at Tianshu (ST 25) and Fujie (SP 14) plus electroacupuncture, while the control group was by sham electroacupuncture. The Complete Spontaneous Bowel Movement (CSBM), Bristol Stool Scale (BSS), and Patient Assessment of Constipation Quality of Life (PAC-QOL) were evaluated before and after intervention.Result In the treatment group, the CSBM after 2-week, 4-week, and 8-week treatments and CSBM of the 4-week and 12-week follow-up study were significantly different from that before intervention (P<0.05). In the control group, the CSBM after 8-week treatments was significantly different from that before intervention (P<0.05). The CSBM after 2-week, 4-week, and 8-week treatments and CSBM of the 4-week and 12-week follow-up study in the treatment group were significantly different from that in the control group (P<0.05). The BSS scores were significantly changed after 2-week, 4-week, and 8-week treatments in both groups compared to that before intervention (P<0.01,P<0.05). The BSS scores after 2-week, 4-week, and 8-week treatments in the treatment group were significantly different from that in the control group (P<0.01,P<0.05). The item scores of PAC-QOL (physical discomfort, psychosocial discomfort, worries and concerns, and satisfaction scores and global score) were significantly changed after intervention in the treatment group (P<0.05). The item scores of PAC-QOLA including physical discomfort, worries and concerns, and satisfaction and global score were significantly changed after intervention in the control group (P<0.05). After intervention, the PAC-QOL scores of the treatment group were significant different from that of the control group (P<0.05).Conclusion Deep needling at Tianshu (ST 25) and Fujie (SP 14) plus electroacupuncture is an effective method in treating severe FC.

Objective To evaluate the variation of left atrial ejection force index (LAEFI) and its determinants in patients with primary hypertension. Methods Using two-dimensional and Doppler echocardiography, LAEFI was measured in 28 normal subjects (control group) and 54 patients with primary hypertension (hypertensive group). Taking the mean + 2SD of LAEFI of the control group as the upper cut-off value, the hypertensive group was divided into two subgroups: group I of LAEFI within the normal range and group II above that. Related clinical variables and echo parameters were compared between the two groups, and stepwise regression was made to determine the influencing factors of LAEFI variation in hypertensive group. Results (1) LAEFI of hypertensive group was larger than that of control group. (2) Multivariate regression demonstrated that LAPVI, AGE and LVMI entered the regression equation.Conclusions (1) LAEFI increased in patients with hypertension, indicating that left atrial systolic function is strengthened for compensation. (2) LAPVI, AGE and LVMI are the most important determinants of LAEFI variation in patients with hypertension.

Objective To evaluate the variation of aortic distensibility and its relation with left ventricular concentric remodeling in patients with primary hypertension.Methods Using two-dimensional and Doppler echocardiography,aortic distensibility (AD) and relative wall thickness (RWT),a parameter of left ventricular concentric remodeling were measured in 23 normal subjects (control group) and 32 patients with primary hypertension (hypertensive group).Taking the mean- 1.645 s of control group as the normal cut off value of AD,the hypertensive group was divided into two subgroups: group I with AD value lower than normal and group II within the normal range.Related clinical variables and echo parameters were compared between the two groups and stepwise regression was made to determine the relationship between AD and RWT.Results AD of hypertensive group was lower than that of control group; Univariate analysis showed that AD was significantly related to RWT,age and systolic blood pressure in hypertensive group; Multivariate regression demonstrated that RWT,age and systolic blood pressure entered the regression equation.Conclusions Aortic distensibility decreased in patients with hypertension and its reduction showed close relations with concentric remodeling of left ventricle,aging and increased systolic blood pressure.

Objective To explore the value of integrate d backscatter technique (IBS) in quantitative evaluation of myocardial viability and left ventricular remodeling (LVRM) in patients with acute myocardial infarction(AMI). Methods In 18 healthy subjects (control group) and 36 patients with first onset AMI, IBS images were obtained at apical long axis, 2- and 4-chamber views within one week, at 1 and 3 months after percutaneous intracoronary stenting. The dynamic changes of IBS and its cyclic variation (CVIB) at middle segments of six different walls of left ventricle were compared with normal control, and the relations of these changes with the remodeling and the function of left ventricle were analyzed. Results Based on the existence of LVRM, the patients were divided into two groups: group A without LVRM and group B with LVRM. In group A, IBS of both infarct and non-infarct segments were not significantly different from control group within one week while CVIB of infarct segments decreased significantly than that of control group. CVIB improved gradually with the recovery of LV function whereas IBS showed no change. In group B, IBS of infarct segments increased significantly than that of control group and CVIB of both infarct and non-infarct segments decreased significantly. IBS of both infarct and non-infarct segments increased gradually while only CVIB of non-infarct segments recovered. Meanwhile, ventricular aneurysm appeared and LV enlarged gradually with LV function not improved. Conclusions The dynamic changes of IBS are different in patients with AMI from normal subjects and it may be clinically useful to evaluate myocardial viability quantitatively and predict LVRM and the recovery of LV function.

Objective To report one stage treatment of chronic tibial defection in children. Methods 19 children with chronic tibial defection were treated in our department when their infection was at the stage of latency. First their focuses of infection were cleared away. Next a piece of fibula with vessel pedicles whose length was equal to that of the tibial defect was taken as the support to restore the length of the tibia. Then the external fixators were applied and a mixture of antibiotic and Lebone powder was used to fill the defect. Results In 15 cases the incision healed at one stage, slight infection occurred in 4 cases whose incisions healed after treatment of 3 to 4 weeks. Follow-ups of 0.5 to 1.5 years showed that no infection occurred again, bone defects healed well and limb function was satisfactory. Conclusion To treat chronic tibial defection in children at one stage, combined therapy should be applied. Grafting of fibula with vessel pedicles and Lebone under external fixators is a good one, because it can shorten the treatment time at no cost of satisfactory effects.

ObjectiveTo establish an ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for detecting the bile acid expression in serum and to explore the relationship between the bile acid spectrum and the obesity.Methods After pre-treatment through solid phase extraction,serum samples were chromatographed by gradient elution on an UPLC HSS T3 column,and then mass spectrometric analysis of bile acid was performed by multiple reaction monitoring ( MRM ) mode with negative electrospray ionization (ESI).And its methodology performance, including specificity,linearity,sensitivity,imprecision and recovery,were verified according to the guideline of State Food and Drug Administration (SFDA).Furthermore,serum samples from 10 simple obesity subjects and 10 healthy controls were analyzed with this establishedmethod, and ranksum test was used for statistical analysis.Results This established UPLC-MS/MS method could simultaneously quantify 14 bile acid subgroups in serum sample,its analytical linear range was 10 - 20 000 nmol/L.For different bile acid subgroups,the limit of detection (LOD) was 0.02 - 7.90 nmol/L,limit of quantitation (LOQ) was 0.07 -44.20 nmol/L; and its within-day coefficient of variation (CV) was 0.35％ - 12.41％,between-day CV was 1.11％ - 13.04％ ; the relative recovery ratio of this method was 89.8％ - 114.6％.Some differenceswere observed in chromatogram between obesity and control subjects,and both free bile acid and conjugated bile acid concentrations in obesity subjects [ 0.49 ( 0.45 - 1.90 ),1.44 ( 0.84 - 3.72 ) μmol/L ] were lower than them in control subjects [0.98(0.53 -3.06),1.99(0.67 -2.88) μmol/L],but the difference was not significant ( Z =- 0.958,- 0.801,P ＞ 0.05 ).ConclusionsThis established UPLC-MS/MS method can efficiently differentiate and quantify 14 bile acid subgroups,and is characterized with broad analytical measurement range,good analytical sensitivity and precision.This method can be applied for the free and conjugated bile acid analysis in clinical specimens.

Objective To evaluate ultrasound combined with thri-operators and the blood-oxygen functional image (TOBOFI) technology in diagnosis of breast cancer in young women. Methods 221 breast tumor patients under 45 years old were selected to analyze their TOBOFI and ultrasonographic characteristics respectively. Based on pathological result, the two diagnostic methods were compared to determine the accurate rate. Results Of all the 221 patients, 82 (37.1%) were malignant and 139 benign. The sensitivity of TOBOFI and ultrasonography was 90.24% and 83.57% respectively, while the accurate rate of the two was 89.14% and 84 62% respectively. Accurate rate was improved to 93.90 % (77/82) if the two were combined. Conclusion TOBOFI has unique clinical value in diagnosing breast cancer in young women, especially in combination with ultrasound.