The experiment on end-to-side anastomosis of inferior superficial epigastric arteriae and femoralarteriae in the cases of 64 rabbits were accomplished. The vessels used in all the instances were less than 1.0 mm in diameter. The rabbits were divided into two groups. 29 of them were used for study of anastomosis angle, and 35 of them for the study anastomosis orifice of side wall. On same one animal both right and left anastomoses were performed at the same time but with different angles of 45? or 90?, and different size of orifice (with side orifice equal to end one, or side one larger than end one) so as to form a contrast.The results revealed no significance in patancy rates either at the angle of 45? or 90?anastomoses, or between the two above-mentional orifice anastomoses. But the average blood flow of the angle of 45? anastomosis and the side orifice equal anastomosis were significantly higher than of the 90? anastomosis and the side orifice larger anastomosis respectively. The advantage at the angle of 45? was shown in chance of thrombosis and the size of thrombus. Therefore, it is suggested we should choose the angle of 45? and the side orifice equal in end-to-side anastomosis.A method of making side wall orifice by "Tactional Suture and Needle" was recommended.In transferring the free skin flaps by using end-to-side anastomosis, four su-c cessful clinical cases were reported in the paper.

Objective To study the injury percentage of fat cells from human bodies by three methods and four suctioned negative pressures. Methods Fat tissue was removed from lower abdomen by excision, aspiration and suction. A pair of sharp scissors was used to cut excisied adipose tissue into 3mm diameter fat particles. A 4 mm diameter pipet and four negative pressures (-25 kPa, -50 kPa, -76 kPa and -101 kPa) were used to suction fat tissue. The samples were fixed in 10 % buffered formalin, stained with hematoxylin eosin and Wilder's silver methods. The fat cells were observed under light microscope. Results The injury percentage of fat cells removed by three methods were (8.6? 1.8) %, (15.7?2.4) % and (38.4?4.2) % respectively;all of them had significant differences among them. The injury percents of fat cells suctioned under-25 kPa,-50 kPa, -76 kPa and -101 kPa were (26.3?2.5) %, (27.6? 2.6) %, (36.8?3.5) % and (68.9?7.4) % respectively. There were no significant differences between the first two but there were among other groups. Conclusions The injury extent of fat cells has significant differences among 3 methods, in which the excision is the least and the suction is the most serious. The injury extent of fat cells has no significant differences when the pressure is lower than -25 kPa. But when the pressure is higher than -50 kPa, the higher the pressure, the more serious the injury extent.

Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.

Objective To study and solve the repairing and reconstruction characteristic for chronic radioactivity ulceration on chest wall.Methods Using transverse rectus abdominis musculocutaneous flaps (TRAM) to repair and reconstruct 12 cases serious chronic radioactivity ulceration after radical operation of mastocarcinoma.Including 6 cases using single pedicle TRAM flaps, 6 cases using double pedicle TRAM flaps.Results All 12 cases were applied successfully with 100% survived and were followed-up for 1 to 4 years.There were better colour, texture, elasticity on flap and obviously improvement for cicatricial tissue round ulceration.Conclusion It is better choice for repairing chronic radioactivity ulceration on chest wall with transverse rectus abdominis musculocutaneous flaps and it is also reliable method for flap circulation using double pedicle TRAM flaps by vascular anastomosis.

The aim of this study was to investigate if there are abnormal fibroblasts derived from the skin surrounding keloids in order to have a better understanding for keloid progression. All the samples were used for cell culture. Flow cytometry was used to compare the apoptotic rate of fibroblasts derived from keloid and its surrounding skin, when it was cultured in serum-deprived medium for 24 hours or was induced by Fas antibody. After cultured in serum-deprived medium for 24 hours, the apoptotic rate of fibroblasts derived from the surrounding skin of keloid increased to an amount between that of normal skin and keloids. The apoptotic rate of normal skin fibroblasts increased more than that of keloids. Moreover, when induced by Fas antibody, the apoptotic rate of fibroblasts derived from the surrounding skin increased not so high as that of normal skin(P0. 05). Therefore, at least there are some fibroblasts in the surrounding skin of keloids, in which apoptosis can not be induced as in normal skin.

Ligustri Lucidi Fructus and its active constituents,ursolic acid and salidroside,have insulin-like effect to produce positive action on skeletal muscle:,It can improve the utilization of carbohydrate and lipid in muscle,increase synthesis of muscular glycogen and protein,muscular weigh,size of fast-and slow-muscle fibers,and accelerate transformation fast-muscle fiber toward slow-muscle fiber.Ursolic acid stimulates muscle satellite cells proliferation,myogenic differentiation of myoblasts,enhances neomyogenesis in skeletal muscle,and promotes skeletal muscle rejuvenation.In addition,Ligustri Lucidi Fructus and its active constituents down-regulating repression of atrogin-1 and muscle ring-finger protein-1 (MuRF-1),and anti-oxidation and anti-inflammation,it may protect and treat for muscle atrophy and fatigue-induced muscle injury.

Objective To compare hemostatic effect of bilateral uterine artery superior branch ligation and gauze intrauterine filling two methods for the placenta previa cesarean section bleeding.Methods 100 patients with bleeding in cesarean section were randomly divided into observation group (50 cases) and control group (50 cases).The patients in the observation group were treated with bilateral uterine artery ascending branch,and the control group was treated with gauze.The number of blood transfusion,the time of operation,the amount of bleeding,the time of hemostasis,the number of hysterectomy and the occurrence of complications were compared between the two groups.Results The success rate of hemostasis in the observation group (98.0％) was higher than that in the control group (86.0％),the difference was statistically significant (x2 =4.891,P ＜ 0.05).The bleeding volume and hemostasis time were (577.8 ± 55.9) mL,(19.2 ± 10.2)min,the differences between the two groups were statistically significant (t =2.50,3.25,all P ＜ 0.05).The operation time between the two groups had no significant difference (P ＜ 0.05).The transfusion rate and incidence rate of complications in the observation group were 8.0％ and 6.0％,which were significantly lower than those in the control group (22.0％,20.0％),and the differences were statistically significant (x2 =3.843,4.332,all P ＜ 0.05).Conclusion The use of bilateral uterine artery esophageal branching in the placenta previa is effective in controlling bleeding,it has rapid bleeding and low incidence of complications,and is worthy of clinical application.

Objective To investigate the effect of salbutamol aerosol combined with magnesium sulfate on IL-2, IL-4, IL-5, IFN-γand T lymphocyte subsets in pediatric asthma patients.Methods 38 pediatric asthma patients were selected and randomly divided into control group and experimental group.Control group was treated by clinical routine method.Experimental group was treated by salbutamol aerosol combined with magnesium sulfate.The IL-2, IL-4, IL-5, IFN-γ, T lymphocyte subsets, blood pressure, heart rate, respiratory and clinical effects were observed and compared.ResuIts Compared with control group, the serum levels of IL-2 and IFN-γwere higher(P<0.05).The IL-5, IL-4 level were lower(P<0.05).The CD3 +, CD4 +, CD4 +/CD8 +level were higher(P<0.05).The serum CD8 +were lower(P<0.05).The total efficiency were higher(P<0.05).ConcIusion Salbutamol aerosol combined with magnesium sulfate can effectively regulate T lymphocyte subsets proportion and cytokine levels in asthmatic children, enhance immunity, and improve the clinical symptoms.

Radix Ginseng and Rhizoma Chuanxiong were identified and the content of ginsenoside Rg_1 was determined by TCL scanning. The results showed that the content of ginsenoside Rg_1 ranged between 0.01770％ and 0.01826％, the recovery rate of ginsenoside Rg_1 was 99.94％ and the coefficient of variation derived from samples was 1.659％. This method is found to be stable, accurate and reliable.

Objective To explore the application and formulas of the tumescent technique in the ultrosonic liposuction for better and more predictable reseults. Methods The tumescent technique formulas were present and analysed through 318 cases of ultrosonic liposuction. Results The following regimen was recommended as a result of the above studies: Lactated Ringer's solution or nosmal sodium was the fluid of choice; Lidocaine (500 mg/l) was a reference anesthetic. The maximal safe dose recommended for liposuction was 35 mg/kg body weight and total dose of epinephrine was 2～3 mg/l. But its concentation should be lower than 1:1 000 000. Concentration of sodium bicarbonate was 500 mg/l, and higer doses were of no value but harmless. Conclusions Safer formulas are provided for the tumescent technique administration in the ultrosonic liposuction. The tumescent technique may reduce potential complications related to ultrosonic liposuction