The experiment on end-to-side anastomosis of inferior superficial epigastric arteriae and femoralarteriae in the cases of 64 rabbits were accomplished. The vessels used in all the instances were less than 1.0 mm in diameter. The rabbits were divided into two groups. 29 of them were used for study of anastomosis angle, and 35 of them for the study anastomosis orifice of side wall. On same one animal both right and left anastomoses were performed at the same time but with different angles of 45? or 90?, and different size of orifice (with side orifice equal to end one, or side one larger than end one) so as to form a contrast.The results revealed no significance in patancy rates either at the angle of 45? or 90?anastomoses, or between the two above-mentional orifice anastomoses. But the average blood flow of the angle of 45? anastomosis and the side orifice equal anastomosis were significantly higher than of the 90? anastomosis and the side orifice larger anastomosis respectively. The advantage at the angle of 45? was shown in chance of thrombosis and the size of thrombus. Therefore, it is suggested we should choose the angle of 45? and the side orifice equal in end-to-side anastomosis.A method of making side wall orifice by "Tactional Suture and Needle" was recommended.In transferring the free skin flaps by using end-to-side anastomosis, four su-c cessful clinical cases were reported in the paper.

Objective To study the injury percentage of fat cells from human bodies by three methods and four suctioned negative pressures. Methods Fat tissue was removed from lower abdomen by excision, aspiration and suction. A pair of sharp scissors was used to cut excisied adipose tissue into 3mm diameter fat particles. A 4 mm diameter pipet and four negative pressures (-25 kPa, -50 kPa, -76 kPa and -101 kPa) were used to suction fat tissue. The samples were fixed in 10 % buffered formalin, stained with hematoxylin eosin and Wilder's silver methods. The fat cells were observed under light microscope. Results The injury percentage of fat cells removed by three methods were (8.6? 1.8) %, (15.7?2.4) % and (38.4?4.2) % respectively;all of them had significant differences among them. The injury percents of fat cells suctioned under-25 kPa,-50 kPa, -76 kPa and -101 kPa were (26.3?2.5) %, (27.6? 2.6) %, (36.8?3.5) % and (68.9?7.4) % respectively. There were no significant differences between the first two but there were among other groups. Conclusions The injury extent of fat cells has significant differences among 3 methods, in which the excision is the least and the suction is the most serious. The injury extent of fat cells has no significant differences when the pressure is lower than -25 kPa. But when the pressure is higher than -50 kPa, the higher the pressure, the more serious the injury extent.

Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.

Objective To evaluate the efficacy of total hip replacement in patients with ankylosing spondylitis(AS) and to solve the problem often encountered during surgery. Methods One hundred and twelve hips of 98 patients with AS undergone total hip replacement were reviewed. The mean duration of follow up was 4.2 years(range, 1.5 to 8 years). The pre and postoperatively comparative study on pain, range of motion, correction of deformity and total function evaluation were conducted. Results Pain relief obtained in all but 4 hips. Mean range of motion was improved from 37.5? to 69.5? and the flexion deformity of the involved hip was corrected from mean 38.2? to 5.7?， all of the patients could take care of daily living by themselves. Complications included penetrating fracture of proximal femur in 4, sciatic nerve injury in 3, early dislocation of the hip in 2, disassociation of the acetabular component in 2 and sinking of the femoral stem in 23. Conclusion Total hip replacement is a very important and effective treatment of choice for patients with AS. The modified combination of anterolateral and lateral approach used by the authors facilitated release of contracture of the soft tissue anterior to the hip joint and correction of flexsion deformity of the hip. In case of multiple joint involvement, the surgery should be performed on the most severely involved one and the next surgery, if required, should be scheduled later within 3 to 6 months.

Objective To explore the application and formulas of the tumescent technique in the ultrosonic liposuction for better and more predictable reseults. Methods The tumescent technique formulas were present and analysed through 318 cases of ultrosonic liposuction. Results The following regimen was recommended as a result of the above studies: Lactated Ringer's solution or nosmal sodium was the fluid of choice; Lidocaine (500 mg/l) was a reference anesthetic. The maximal safe dose recommended for liposuction was 35 mg/kg body weight and total dose of epinephrine was 2～3 mg/l. But its concentation should be lower than 1:1 000 000. Concentration of sodium bicarbonate was 500 mg/l, and higer doses were of no value but harmless. Conclusions Safer formulas are provided for the tumescent technique administration in the ultrosonic liposuction. The tumescent technique may reduce potential complications related to ultrosonic liposuction

The aim of this study was to investigate if there are abnormal fibroblasts derived from the skin surrounding keloids in order to have a better understanding for keloid progression. All the samples were used for cell culture. Flow cytometry was used to compare the apoptotic rate of fibroblasts derived from keloid and its surrounding skin, when it was cultured in serum-deprived medium for 24 hours or was induced by Fas antibody. After cultured in serum-deprived medium for 24 hours, the apoptotic rate of fibroblasts derived from the surrounding skin of keloid increased to an amount between that of normal skin and keloids. The apoptotic rate of normal skin fibroblasts increased more than that of keloids. Moreover, when induced by Fas antibody, the apoptotic rate of fibroblasts derived from the surrounding skin increased not so high as that of normal skin(P0. 05). Therefore, at least there are some fibroblasts in the surrounding skin of keloids, in which apoptosis can not be induced as in normal skin.

Objective To introduce a method of comprehension correction for secondary lip-nasal deformities after operation of the completely unilateral cleft lip. Methods The correction consisted of the following procedures: reposition of the columella and nasal alar crus by one tractional suture with nostril base narrowing; modification of flat nostril by a MT-plasty at the edge of naris; arising of the nasal alar and nasal dorsor by ePTFE implantation; reconstruction of the Cupid's bow by M type incision and vermilion advancement; repair of the vermilion deficit with the hinge mucosal flap and bipedicle mucosal flaps of the lower lip. Results Twenty-one cases with secondary lip-nasal deformities were successfully corrected by these comprehensive methods. Ten of them were followed-up for three months to 1 year with satisfactory effects. Conclusion The comprehensive methods can be used to correct satisfactorily the secondary lip-nasal deformities after operation of the completely unilateral cleft lip.

Objective To investigate the course of the mandibular branch of the facial nerve and to discuss its clinical significant in the rhytidectomy. Methods The distribution of the mandibular branch was observed in 30 halves of the fifteen candaveric specimens (ten antiseptic cadaveric specimens and five fresh cadaveric specimens). Results The mandibular branch could be divided into the isolated branch type and shared branch type, after it exited from the parotid gland. 63.33 % mandibular branch was found (2.1?0.7) cm superior to the palpable edge of the mandibular bone; 23.33 % mandibular branch was along the edge of the bone; and 13.33 % was found (1.8?0.5)cm inferior to the palpable edge of the bone. Conclusion The distribution of the mandibular branch locates in the area that is a digit superior and inferior to the lower border of the mandibular bone, which arises from the angle of the mandibular bone. The dissection beneath the SMAS-platysma should be with caution of the injury of the ramification of mandibular branch in the anterior border of the masseter muscle.[

Objective To evaluate the therapeutic efficacy of congenital muscular torticollis (CMT) by two different methods. Methods A total of 86 patients with congenital muscular torticollis in recent 7 years were involved in this study, including 69 cases under 12 years old and 17 cases over 12. Two different operation methods, incision of inferior extremity of sternocleidomastoid muscle and incision of amphi-sternocleidomastoid muscle with post-operative traction, were alternatively chosen according to the ages and deformed degrees of the patients. Results 72 patients were followed-up for 1 to 7 years (averaged 4.5 years). Based on the effect of function and cosmetology in the group of 57 cases under 12 years old, there were 52 cases (91.2 %) excellent, 5 cases (8.8 %) good and no case with side-effects. Meanwhile, in the group of 15 cases over 12 years old, there were 11 cases (73.3 %) excellent, 4 cases (26.7 %) good and no case with side-effects. The effect was satisfactory. Conclusion Different patients with congenital muscular torticollis should be treated with different therapeutic methods.

Objective To establish the biostereometric system of breast volume measurement and to estimate the precision and reliability of the designed system. Methods Biostereometric system of breast volume measurement was built upon the biostereometrics based on computer stereo vision and image processing. One laser projector was used as illuminating source, two CCD cameras were mounted for photo-taken, a series of parallel points were provided through a piece of grating. The volumes of 12 female breast models were tested by water displacement (golden standard) and biostereometric analysis. Results The maximum size of marks we photographed was within 120 mm?140 mm?80 mm. The characters of the system was rapid, non-contact and noninvasive. The validity and reliability of determination of breast volume between biostereometric system and golden standard had no significant difference, compared to water displacement (P=0.473). Conclusions Biostereometric system of breast volume measurement will be suited for the clinical application. The study offers a noninvasive, non-contact, rapid and accurate morphologic method and provides a new theory foundation for morphological analysis.