The experiment on end-to-side anastomosis of inferior superficial epigastric arteriae and femoralarteriae in the cases of 64 rabbits were accomplished. The vessels used in all the instances were less than 1.0 mm in diameter. The rabbits were divided into two groups. 29 of them were used for study of anastomosis angle, and 35 of them for the study anastomosis orifice of side wall. On same one animal both right and left anastomoses were performed at the same time but with different angles of 45? or 90?, and different size of orifice (with side orifice equal to end one, or side one larger than end one) so as to form a contrast.The results revealed no significance in patancy rates either at the angle of 45? or 90?anastomoses, or between the two above-mentional orifice anastomoses. But the average blood flow of the angle of 45? anastomosis and the side orifice equal anastomosis were significantly higher than of the 90? anastomosis and the side orifice larger anastomosis respectively. The advantage at the angle of 45? was shown in chance of thrombosis and the size of thrombus. Therefore, it is suggested we should choose the angle of 45? and the side orifice equal in end-to-side anastomosis.A method of making side wall orifice by "Tactional Suture and Needle" was recommended.In transferring the free skin flaps by using end-to-side anastomosis, four su-c cessful clinical cases were reported in the paper.

Objective To study the injury percentage of fat cells from human bodies by three methods and four suctioned negative pressures. Methods Fat tissue was removed from lower abdomen by excision, aspiration and suction. A pair of sharp scissors was used to cut excisied adipose tissue into 3mm diameter fat particles. A 4 mm diameter pipet and four negative pressures (-25 kPa, -50 kPa, -76 kPa and -101 kPa) were used to suction fat tissue. The samples were fixed in 10 % buffered formalin, stained with hematoxylin eosin and Wilder's silver methods. The fat cells were observed under light microscope. Results The injury percentage of fat cells removed by three methods were (8.6? 1.8) %, (15.7?2.4) % and (38.4?4.2) % respectively;all of them had significant differences among them. The injury percents of fat cells suctioned under-25 kPa,-50 kPa, -76 kPa and -101 kPa were (26.3?2.5) %, (27.6? 2.6) %, (36.8?3.5) % and (68.9?7.4) % respectively. There were no significant differences between the first two but there were among other groups. Conclusions The injury extent of fat cells has significant differences among 3 methods, in which the excision is the least and the suction is the most serious. The injury extent of fat cells has no significant differences when the pressure is lower than -25 kPa. But when the pressure is higher than -50 kPa, the higher the pressure, the more serious the injury extent.

Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.

Objective To compare the operation and clinical effects of total hip arthroplasty and hemiarthroplasty in the treatment of fracture of femoral neck in elderly patients.Methods 58 elderly patients( ≥85 ) with femoral neck fracture were divided into observation group(n=39) and control group(n=19).The observation group was treated by hemiarthroplasty and the control group was treated by total hip arthroplasty.The operation time,blood loss,hospital stay,postoperative hip functional score and complications were observed and compared between the two groups.Results The operation time and blood loss of the observation group were(71.6 ±9.3)min and (266.8±113.5) ml respectively,which were significantly less than those of the control group ( t=7.89,3.43,all P ＜ 0.05 ).Therer was no significant difference of the hospital stay,postoperative hip functional score and incidence rate of complications between the two groups.Conclusion Hemiarthroplasty was an ideal rmethod in treating fracture of femoral neck in elderly patients,which was effective and simple.

Radix Ginseng and Rhizoma Chuanxiong were identified and the content of ginsenoside Rg_1 was determined by TCL scanning. The results showed that the content of ginsenoside Rg_1 ranged between 0.01770％ and 0.01826％, the recovery rate of ginsenoside Rg_1 was 99.94％ and the coefficient of variation derived from samples was 1.659％. This method is found to be stable, accurate and reliable.

The resected specimens of esophageal adenocarcinoma from 73 patients were studied pathohistologically. The results demonstrated stage I in 1 cases; stage IIA 37; stage HB 8 and stage III 27. The incidence of postoperative complications and operative mortality were 4.1% and 1.4% respectively. The overall 5 year survival rate was 28. 3%. The authors pointed out that patients with primary esophageal adenocarcinoma should be operated on early. Even the late stage patients should also be treated surgically, because the esophageal adenocarcinoma is neither sensative to chemotherapy, nor radiotherapy.

Objective To evaluate the therapeutic efficacy of congenital muscular torticollis (CMT) by two different methods. Methods A total of 86 patients with congenital muscular torticollis in recent 7 years were involved in this study, including 69 cases under 12 years old and 17 cases over 12. Two different operation methods, incision of inferior extremity of sternocleidomastoid muscle and incision of amphi-sternocleidomastoid muscle with post-operative traction, were alternatively chosen according to the ages and deformed degrees of the patients. Results 72 patients were followed-up for 1 to 7 years (averaged 4.5 years). Based on the effect of function and cosmetology in the group of 57 cases under 12 years old, there were 52 cases (91.2 %) excellent, 5 cases (8.8 %) good and no case with side-effects. Meanwhile, in the group of 15 cases over 12 years old, there were 11 cases (73.3 %) excellent, 4 cases (26.7 %) good and no case with side-effects. The effect was satisfactory. Conclusion Different patients with congenital muscular torticollis should be treated with different therapeutic methods.

Objective To establish the biostereometric system of breast volume measurement and to estimate the precision and reliability of the designed system. Methods Biostereometric system of breast volume measurement was built upon the biostereometrics based on computer stereo vision and image processing. One laser projector was used as illuminating source, two CCD cameras were mounted for photo-taken, a series of parallel points were provided through a piece of grating. The volumes of 12 female breast models were tested by water displacement (golden standard) and biostereometric analysis. Results The maximum size of marks we photographed was within 120 mm?140 mm?80 mm. The characters of the system was rapid, non-contact and noninvasive. The validity and reliability of determination of breast volume between biostereometric system and golden standard had no significant difference, compared to water displacement (P=0.473). Conclusions Biostereometric system of breast volume measurement will be suited for the clinical application. The study offers a noninvasive, non-contact, rapid and accurate morphologic method and provides a new theory foundation for morphological analysis.

Objective To introduce an improved technique of periareolar incision mammoplasty using gland flap suspending and contour line circum-contraction suturing. Methods Through the periareolar incision, dissection was conducted between the subcutaneous tissue and the glandular tissue to detach the skin flap tent from the gland cone. A vertical glandular flap was formed in the upper portion to suspend the gland, with the gland tissue partially excised medial and lateral to the vertical glandular flap. Continuous contour line circum-contraction suturing was made for 2～3 rounds step by step from the cone bottom to the plateau, i.e. the nipple areola level. The suturing proceeded alternately from the flap tent to the glandular cone. Finally, the skin was sutured along the areolar incision. Results Sixteen breasts of eight patients were operated on using the present technique, including 10 cases of macromastia and 6 of breast ptosis. Satisfactory results were achieved with pretty breast shape and inconspicuous periareolar scar. Conclusion The contour line circum-contraction suturing periareolar incision mammoplasty is an optimal choice for moderate macromastia and breast ptosis.

The aim of this study was to investigate if there are abnormal fibroblasts derived from the skin surrounding keloids in order to have a better understanding for keloid progression. All the samples were used for cell culture. Flow cytometry was used to compare the apoptotic rate of fibroblasts derived from keloid and its surrounding skin, when it was cultured in serum-deprived medium for 24 hours or was induced by Fas antibody. After cultured in serum-deprived medium for 24 hours, the apoptotic rate of fibroblasts derived from the surrounding skin of keloid increased to an amount between that of normal skin and keloids. The apoptotic rate of normal skin fibroblasts increased more than that of keloids. Moreover, when induced by Fas antibody, the apoptotic rate of fibroblasts derived from the surrounding skin increased not so high as that of normal skin(P0. 05). Therefore, at least there are some fibroblasts in the surrounding skin of keloids, in which apoptosis can not be induced as in normal skin.