Hypoxia-inducible factor(HIF)is a pivotal transcription factor for hypoxic response.This article reviews the related characteristics of HIF-1 and its target genes vascular endothelial growth factor and the neuroprotective mechanisrms in cerebral ischemia.

Objective To explore factors affecting diagnosis and treatment compliance in patients with gout leaving hospital,in order to provide a reference for late-stage clinical treatment.Methods 390 patients with gout treated in our hospital were selected from August 2012 to February 2016 for the study,all patients were followed up by telephone,their diagnosis and treatment compliance after discharge was investigated,and the factors affecting diagnosis and treatment compliance in gout patients after discharge investigated were,as well.Results The mean score of treatment adherence of 390 cases of gout patients after discharge was(3.93± 1.78) points.There were 117(30.00％) patients of which their score were ≥6 points for compliers,and 273 (70.00％) patients who do not comply with scores ＜6 points.Disease duration,education level,frequency of gout attacks,marital status and payment of medical expenses were closely related to diagnosis and treatment adherence(P＜0.05).Longer duration,low educational level,payment of medical expenses at their own expense were independent risk factors for poor adherence (P＜0.05).Conclusion We should strengthen the monitoring and follow-up of patients with gout who are with long duration of disease,low educational level,and pay medical expenses from their own pocke tinorder to improve patient diagnosis and treatment adherence,provide references for late-stage clinical treatment.

Objective To explore a new method for establishing an animal model of cochlear ischemia.Methods Thirty guinea pigs were randomized into three groups:normal group,operation group,ischemia group.Anterior inferior cerebellar artery(AICA)was exposed surgically via a skull base approach.In ischemia group,a small piece of filter paper containing FeCl3 was placed upon the AICA to induce thrombus formation.In operation group,AICA was only exposed.In normal group,nothing was done to animal.The blood flow of cochlea(CoBF)was continuously monitored with laser Doppler flowmeter.The thresholds of auditory brainstem response(ABR)were measured before,after each experiment,and one week after operation.The AICAs of ischemia group and operation group were resected and pathological sections were perfomed for observation of the details.Results After the administration of FeCl3,the CoBF decreased to 50% of the base level at 30 min.In the AICA pathological sections of ischemia group,white thrombus composed of cellulose,blood platelets and erythrocytes could be seen.The threshold of ABR in ischemia group increased significantly.Conclusion The method provides a relatively ideal model for ischemis of the inner ear and this model may be helpful in studying the mechanisms of hearing loss caused by chronic microcirculatory disorders.

AIM:Cyclin is a decisive factor of regulating cell cycle,and RNA interference as an effective and specific gene silencing technique,can induce cell express the phenotype of specific gene deficiency. This study is to apply cyclinD1 specific small interfering RNA(siRNA) on inhibiting cyclinD1 gene expression and investigate the effect of cyclinD1 specific siRNA on the cell cycle and multiplication in keloid fibroblasts. METHODS:The experiment was conducted at the Institute of Genetic Engineering(Grade BSL-2) in Southern Medical University from July 2006 to May 2007.①siRNA was designed with siRNA target finder of ambion Company,and synthesized chemically in Shanghai GeneChem,Co.,Ltd. Then double-strand siRNA was obtained following degenerative renaturation. Keloid fibroblasts were sampled from the patients in the Department of Plastic Surgery,Southern Medical University(informed consents were obtained from the patients or their relatives).②The keloid fibroblasts were divided into experimental group and control group. The cyclinD1 specific siRNA was transfected into the keloid fibroblast of the experimental group by the liposome-mediated gene transfection method. The untreated cells were set as controls.③At hours 24,48 and 72 of transfection,light microscope was used to observe cell morphologic change and flow cytometry was used to examine cell cycle. The viable cells were counted by MTT colorimetry and a cell growth curve was drawn. RESULTS:①Abnormal change of cell morphology that became into spherical shape or oval-shape from normal long fusiform after transfection may be the apoptosis or necrosis cells.②The G1 stage of cell cycle extended and the S stage decurtated. At 24,48 and 72 hours after transfection,the radio of G1 stage cell was 60.13%,66.22% and 67.53%,which were all significantly higher than that in the control group(54.53%);the radio of S stage cell(18.25%,17.11% and 11.15%) was also significantly lower than that in the control group(22.31%),indicating that the proportion of the cells blocked in G1 stage and those in S stage decreased in the keloid fibroblast.③siRNA-cyclinD1 inhibited the growth of keloid fibroblasts obviously by using MTT assay,and the cell growth curves indicated that the proliferation of cell transfected with cyclinD1 specific siRNA was inhibited significantly when compared with controls. CONCLUSION:CyclinD1 specific siRNA effectively inhibits the expression of cyclinD1 in keloid fibroblasts thus arresting the cell cycle at G1 stage and enhancing cell apoptosis.

Diffuse sclerosing variant papillary thyroid carcinoma,one of the variants of papillary thyroid carcinoma,nearly account for 0.7％ to 6.6％ of papillary thyroid carcinoma.It is an aggressive tumor that shows higher rates of recurrence,metastasis and persistent disease compares to classic papillary thyroid carcinoma,thus diffuse sclerosing variant papillary thyroid carcinoma needs stricter and more exhaustive operation.Since the incidence of diffuse sclerosing variant papillary thyroid carcinoma is low,many physicians lack of understanding of the disease,it's often missed diagnosis or misdiagnosed.Consequently.In order to let physicians understand more about diffuse sclerosing variant papillary thyroid carcinoma,this paper will elaborate the epidemiology,clinic and pathology features of it.

Behavior guiding teaching method is a new teaching method,It requires putting the emphasis on students'action of learning and thought,which can improve the whole quality of students'.Forensic toxicological analysis'is an important course in the department of forensic,and application of behavior guiding teaching method in the "Forensic Toxicological Analysis" is of help to students'ability development and good teaching results.

Objective To investigate the effects of pulsed electromagnetic fields (PEMF) on osteogenic differentiation in demineralized bone matrix (DBM)-induced human marrow stromal cells (hMSCs).Methods hMSCs were obtained from iliac crest marrow aspirates of a healthy boy aged 14.Then 1?104 hMSCs per well were plated in 8 24-well tissue culture plates,and 1?105 hMSCs per well in 2 6-well plates after they were expanded until passage 2.Those 24-well plates and 6-well plates were divided into 4 groups,cell control group (C group),cell-material group (CD group),cell-PEMF group (CP group) and cell-material-PEMF group (CDP group).CD group and CDP group were added with one DBM (size of 5 mm?5 mm?3 mm),CP and CDP groups were exposed to the PEMF (frequency of 15 Hz,intensity of 5 Gs),and hMSCs were cultivated in subculture medium with full media exchange in every 3 d.Alkaline phosphatase (ALP) activity and osteocalcin (OC) level were performed at 1,7,14 and 21 d,and alizarin red staining and counting of calcium nodules were performed at 21 d.Results Significant differences in ALP activity and OC level were observed between cell control group and the other 3 groups in 7 d after treatment (P

Objective To investigate the means of different anterior surgical approaches for cervicothoracic vertebral tumors, tumor excision procedure and reconstruction. Methods The clinical manifestation of tumors, different operative approaches, surgical procedures and prognosis of 7 cases admitted in our hospital from March, 1999 to May, 2002 were reported. Results After a follow up of 3 to 28 months, neurological function was improved significantly in all cases. One case with angiosarcoma and 2 cases with metastatic adenocarcinoma (C7, C6 7, T1) died of metastasis in other multiple locations and system failure in 3, 8 and 13 months after surgery respectively. No recurrent tumor focus was found at the operative sites during the follow up period. Conclusion The operative approach, surgical procedure and cervicothoracic spine anterior reconstruction should be selected according to the nature and location of tumors.

Objective To investigate the effect of propofol on the liver function of offspring rats de?livered by cesarean section. Methods Twenty?four Sprague?Dawley rats, at 20 days of gestation, weig?hing 230-270 g, were divided into 4 groups ( n=6 each) using a random number table: control group (group C), propofol 2.0 mg∕kg group (group P2), propofol 4.0 mg∕kg group (group P4), and propofol 8.0 mg∕kg group (group P8). In P2, P4 and P8 groups, the corresponding doses of propofol were injected intravenously, and the cesarean section was performed at 30 min after loss of righting reflex. In group C, the pregnant rats were sacrificed by cervical dislocation at the corresponding time point, and the offspring rats were removed immediately and inhaled oxygen sufficiently. The neonatal rats were sacrificed immediate?ly, and the blood samples were taken from the heart for determination of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations, and the livers were removed and cut into sections which were stained with haematoxylin and eosin for microscopic examination of the pathological changes. Results Compared with group C, the plasma ALT and AST concentrations in the offspring rats were significantly increased in P2, P4 and P8 groups (P<0.05). Compared with group P2, the plasma ALT and AST concentrations in the offspring rats were significantly increased in P 4 and P 8 groups ( P<0.01). Compared with group P4, the plasma ALT and AST concentrations in the offspring rats were signifi?cantly increased in group P8 (P<0.01). In P2, P4 and P8 groups, marked pathological changes of liver tissues were observed, and the severity was gradually aggravated in turn in offspring rats. Conclusion Propofol can induce damage to the livers of offspring rats delivered by cesarean section in a dose?dependent manner.

Objective To study the characteristics of maxillary growth after lip and palate repair. Methods Using X-ray cephalometry through 12 landmarks and 12 measured values we had compared the difference between 60 patients with operated cleft lip and palate and 20 normal persons. Results After the operation all patients appeared limits of maxillary growth in different degree and different way. SNA and N-A-Pg [(-4.8?6.31)mm, P 0.05] were smaller than normal in patients of cleft lip. SNA(74.5?4.01)?, P 0.05;(75.1?1.07)?, P 0.01], and N-ANS [(47.3?2.41)mm, P 0.01;(49.8? 1.91) mm, P 0.01] were also smaller than normal in cleft palate and cleft lip and palate. Conclusions The limits of maxillary growth are affected by congenital and operative factors. Lip repair will influence the length of maxilla, and palate repair will influence height and width of maxilla.