The distribution of properdin factor B (Bf)allotypes and their gene frequencies in 81 patients with systemic lupus erythematosus (SLE) was investigated using high voltage agarose gel electrophoresis and subsequent immunofixation, in an attempt to define whether specific Bf allotypes were associated with the disease. An increase in gene frequency of Bf F in the patients with SLE (0.1914) was found in comparison with that in the controls (0.1159), P_(correct)

Five min coronary artery occlusion followed by reperfusion in rats could produce arrhythmias which were rapid in onset & of short duration. The incidences of VT & VF being 100% & 72% respectively. Quinidine & dauricine reduced the incidence & severity of VT significantly; and abolished VF. Tetrandrine could reduce the incidence of VF significantly, but it had no significant effect on the incidence of VT.

Disdvantages of chemotherapy in treating breast cancer were insensitivity or intolerance.There were different opinions on chemotherapy in treating negative axillary lymph notes or positive hormone receptors after breast cancer surgery.The advantages of TCM in treating breast cancer were:there were many Chinese medical oncologists who had rich clinical experiences in treating breast cancer before and during surgery,before and during chemotherapy,before and during radiotherapy and during the consolidation period;treatment according to syndrome differentiation with TCM for different stages of breast cancer had been gradually standardized;a well developed network of academic information provided a good platform for establishing the unified treatment protocols with TCM for different stages of breast cancer.It was indicated that large scale prospective clinical trials with TCM in the treatment of after-surgery breast cancer patients who had negative auxillary lymph notes and positive hormone receptors should be carried out.

Objectives To explore the pathological characteristics, treatment and prognosis of medulloblastoma (MB) in children. Methods Pathological characteristics, treatment outcomes and other clinical data were retrospectively analyzed in 60 children with MB. Results The MB in all 60 children were high-grade tumor. The pathological type was mainly classic (45 cases, 75.00%) and other subtypes were seen in 15 cases (25.00%). Forty-eight (80.00%) children had total resection by micro-surgery, 9 children (15.00%) subtotal resection, and 3 children (5.00%) partial resection. Twenty-seven children were treated by radiotherapy after surgery, 6 children were treated by chemotherapy after surgery, and 5 children were treated with radiotherapy combined chemotherapy after surgery. Thirty-eight cases were followed up. The 2-year survival and disease-free survival rates were 63.16%and 55.26%. The 5-year survival and disease-free survival rates were 26.32%and 18.42%. The children with des-moplastic/nodular type survived significantly longer than the children with large cell anaplastic. The children with total resection survived significantly longer than the children with partial resection. The children with radiotherapy or chemotherapy survived significantly longer than the children without chemotherapy (P<0.05). Conclusions MB in most of children is high-grade tu-mors and the prognosis is poor. The pathological type is related with prognosis. Microsurgical total resection is the first choice of treatment. Postoperative radiotherapy and chemotherapy can increase the survival rate.

Objective To investigate the value of ultrasound-guided Mammotome minimal invasive system in the treatment of breast fibroadenoma.MethodsFrom January 2007 to October 2008,266 benign breast lesions of 183 cases were treated by ultrasound-guided Mammotome minimal invasive system.The diameter of the masses ranged from 4 to 25 mm(≤ 10 mm in 73 cases,10-20 mm in 148,and ≥ 20 mm in 45).ResultsThe 266 lesions in the 183 patients were excised completely by using the Mammotome system.The excision was performed for a mean of 15 times in each case(ranged from 5 to 44 times) with an average operation time of 26 minutes(10 to 60 minutes).No complication was found after the surgery except slight subcutaneous ecchymosis in 2 cases.The length of the incisional scar,which was not apparent,ranged from 2.5 to 4 mm.The 183 cases were followed up by ultrasonography for 2 to 22 months(mean,12 months),during which their BI-RADS scales ranged from Ⅰ to Ⅱ,no local recurrence was found.ConclusionsUltrasound-guided Mammotome minimally invasive system is feasible for total resection of breast fibroadenoma with appropriate sizes.The technique leads to fewer complications and good cosmetic results.Further studies on the system are necessary for the treatment of multiple breast fibroadenomas.

ObjectiveTo investigate the curative effect of the treatment for the distal humeral shaft fracture with double steel plate internal fixation. Methods A total of 60 individuals who were humeral shaft fractures were included from March 2009 to April 2014 in the second people's hospital of Yunnan province. 28 of them were treated using double plate fixation(group A)and 32 of them were treated using single-plate treatment (group B). Blood loss, hospital stay, postoperative drainage, healing time and Mayo elbow performance score were compared between the two groups. Results(1)Blood loss, hospital stay and postoperative drainage showed no differences between two groups. However, the healing time between these two groups showed difference(P < 0.05).(2)MEPS score results showed a significantly difference in the excellent rate of patients between groups A and B(96.4% and 75%, respectively,P < 0.05). Conclusion The double plate fixation and single plate fixation for treatment of humeral shaft fracture showed no differences in operation time,blood loss,postoperative drainage and hospital stay. However,the double plate fixation showed a shorter healing time and better efficacy. Thus,the double plate fixation of humeral shaft fracture can contribute to obtain the rigid internal fixation,early functional exercise and better clinical efficacy.

Objective To analyse effect of dobutamine on C-reactive protein,D-dimer,BNP and blood gas analysis in children with neonatal pulmonary hypertension.Methods 46 patients who were diagnosed with neonatal pulmonary hypertension in our hospital were collected.All patients were randomly divided into experimental group and control group, 23 cases in each group.Control group was treated with sedation, maintenance of water electrolyte balance, blood pressure, anti-infection,experiment group was treated with dobutamine hydrochloride injection 5μg/(kg· min) intravenous infusion, continuous for 48 h on the basis of conventional therapy, after treatment,the levels of peripheral blood C-reactive protein, D-dimer and BNP and blood gas analysis effect were detected in all patients.Results Compared with control group post-treatment, the serum level CRP,D-dimer, BNP were lower in experimental group ( P <0.05 );the PaO2 and SpO2 levels were higher, the PAH level was lower in experimental group ( P <0.05 ) . Conclusion The dobutamine can significantly reduce the serum CRP, plasma D-dimer and BNP levels in children with neonatal pulmonary hypertension, reduce pulmonary hypertension.

BACKGROUND: Cervical sympathetic ganglia block accelerates the re covery of the homeostasis of organic nervous-endocrine-immune system, butit is still unclear whether it can suppress the imbalance of homeostasis in duced by post-traumatic stress disorder. OBJECTIVE: To observe the effect of cervical sympathetic ganglia blockon the mortality of mice with combined radiation and burn injury, andwhether it can become an easy and effective method to treat secondarydamage after serious trauma. DESIGN: A randomized grouping design, an animal controlled experiment. SETTING: Department of Anesthesiology, Guangzhou General Hospital, Guangzhou Military Area Command of Chinese PLA.MATERIALS: The experiments were carried out in the Institute of Combined Injury, the Third Military Medical University of Chinese PLA between February 2004 and July 2005. Totally 160 Kunming mice were randomly divided into control group (n=50) and cervical sympathetic ganglia block group (n=50). In the control group, the mice were only induced to models of combined radiation and bum injury, and treated with injection of 0.3 mL saline at cervical part. In the cervical sympathetic ganglia block group, the mice were induced to models of combined radiation and burn injury, and then treated with cervical sympathetic ganglia block, once a day for 14 days continuously.METHODS: Methods to induce injury in the animals: ① Radiation injury: The mice were given even radiation of 60Coγ ray (5 Gy) at a distance of 1.5 m to the whole body, the rate of absorptive dosage was (5.17-5.33) mGy/s. ② Burn injury: After the radiation injury, coagulated gasoline was smeared on the back and burnt for 8 s to induce degree Ⅲ burn injury of 15% of the total body surface, which was proved by the pathological section. Methods of cervical sympathetic ganglia block: Cervical sympathetic ganglia block was given bilaterally, and then the mice were injected with 0.2 mL lidocaine (5 g/L), and it was observed whether the symptoms similar to Horner syndrome (hyperemia of conjunctiva, drooping eyelid,blushing, smaller eyeslit) occurred or not at 5 minutes after injection.MAIN OUTCOME MEASURES: The mortality at 2, 5, 7, 10, 20 and30 days after injury and the changes of the numbers of red blood cells,white blood cells and blood platelet in peripheral blood at 7, 14 and 21 days after injury were observed in both groups. The effects of cervical sympathetic ganglia block on the levels of tumor necrosis factor-alpha (TNF-α),interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum at 3, 6 and 14days after combined radiation and burn injury were also observed.RESULTS: All the 160 mice were involved in the analysis of results without deletion. ① Compared with the control group, the mortalities at 5,7, 10, 15, 20 and 30 days in the cervical sympathetic ganglia block group were significantly decreased [control group: 8%, 22%, 32%, 54%, 74%,82%, 90%; cervical sympathetic ganglia block group: 8%, 14%, 16%, 22%,28%, 34%, 56%]. ② Compared with the control group, the numbers of red blood cells, white blood cells and blood platelets in peripheral blood at 7,14 and 21 days after injury in the cervical sympathetic ganglia block group were significantly increased [at 21 days: red blood cells: 23.21×1012 L-1, 14.58×1012 L-1; blood platelet: 16.87×1011 L-1, 12.57×1011 L-1; white blood cells: 20.65×109 L-1, 14.58×109 L-1]. ③ The levels of TNF-α, IL-1β andIL-6 in serum at 3, 6 and 14 days after injury in the cervical sympathetic ganglia block group were significantly decreased as compared with those in the control group [at 14 days: TNF-α: 189, 365 ng/L; IL-1β: 14, 23 ng/L;IL-6: 70, 132 ng/L].CONCLUSION: Cervical sympathetic ganglia block can significantly decrease the mortality of animals with combined radiation and burn injury,and it is an easy and effective method to treat serious trauma, and the mechanism may be realized through accelerating the recovery of hematopoietic function and suppressing the excessive inflammatory reaction.

Objective:To explore the correlation betWeen deceleration capacity of heart rate (DC)and heart rate var-iability (HRV)in patients With essential hypertension (EH)complicated coronary heart disease (CHD).Methods:DC and HRV Were monitored in 81 patients With EH + CHD (EH + CHD group)and 80 healthy volunteers (healthy control group)respectively.Results:Compared With healthy control group,there Were significant decrease in DC [(11.99±4.93)ms vs.(6.62±1.83)ms],standard deviation of normal to normal RR intervals calculated o-ver the 24 h period [SDNN,(129.94±15.22)ms vs.(63.66±12.13)ms],root-mean square of differences be-tWeen successive normal to normal intervals [rMSSD,(55.52±9.03)ms vs.(28.25±8.81)ms]and adjacent nor-mal RR interval difference > 50ms stroke accounted for a percentage of 24h total RR interval [PNN50,(13.99± 5.63)% vs.(7.29±1.11)%]of HRV in EH + CHD group,P <0.05 all;Pearson correlation analysis indicated that DC Was positively correlatedWith SDNN,rMSSD and PNN50 in EH+CHD patients (r =0.571,0.662,0.763, P <0.05 all).Conclusion:There is positive correlation betWeen deceleration capacity of heart rate and heart rate variability in patients With hypertension complicated coronary heart disease,Which can be jointly used as evidence for diagnosis and treatment.