Objective To investigate the quality of life of the elderly living at a nursing home and its influencing factors.Methods One hundred and sixty elderly living at one nursing home of Shanghai completed 36-item Short Form (SF-36) Health Survey,Social Support Rate Scale,Geriatric Depression Scale (GDS) and UCLA Loneliness Scale (UCLA-LS) to evaluate the factors that influenced the quality of life and the relationship between quality of life,social support,depression and loneliness in December of 2011.Results The mean age of the elderly was (82.0 ± 8.8).The polynary linear step-wise regression analysis showed that physical function of the elderly was mainly influenced by having children or not,frequency of physical activity and self-assessment of health condition.Role-physical,social function,vitality and role-emotional of the elderly were mainly influenced by age,frequency of physical activity and selfassessment of health condition.Bodily pain of the elderly was mainly influenced by sex and self-assessment of health condition.General health of the elderly was mainly influenced by age,frequency of physical activity,chronic diseases,and self-assessment of health condition.Mental health of the elderly was mainly influenced by age,living status,frequency of physical activity and self-assessment of health condition.Subjective support was positively related to role-emotional and mental health.However,the life quality of the elderly was positively correlated with objective support,utilization of support,total score of social support and each dimension of SF-36.The depression and loneliness of the elderly were negtively related to each dimension of SF-36.Conclusions Multiple factors may influence the quality of life of the elderly.The whole society and family members should make concern and set up better environment to for the elderly.

Objective To discuss the effects of nursing intervention under the guidance of health belief on enteroscopy compliance of colorectal cancer high-risk population. Methods A total of 260 people, who were clearly identified as colorectal cancer high-risk population during preliminary screening of 2 communities in Nankai District, Tianjin but had not finished enteroscopy, were selected and divided them into intervention group and control group with 130 people per group by random digits table method. In the intervention group, subjects received nursing intervention with guidance of the health belief model for a year, while in the control group, subjects simply received regular propaganda and education about colorectal cancer screening. Results After intervention, the cognitive levels in colorectal cancer screening, enteroscopy screening, high-risk factors was (9.16±2.95), (14.55±3.41), (14.25±4.26) points in intervention group and (7.28 ± 3.26), (12.23 ± 3.72), (13.12 ± 4.11) points in control group, and there was significant difference between two groups (t=4.855, 5.228, 2.180, P<0.01 or 0.05). After intervention, the scores of health belief including susceptibility, benefits, barriers, self-efficacy was (7.74 ± 1.50), (13.20 ± 2.45), (6.59 ± 1.91), (23.54±3.16) points in intervention group, and (6.68 ± 1.47), (11.58 ± 2.15), (8.42 ± 1.99), (20.82 ± 3.02) points in control group, and there was significant difference between two groups(t=5.730, 5.682,-7.561, 7.096, P<0.01). The rate of enteroscopy screening was 20.47%(26/127) in intervention group and 8.87%(11/124) in control group, and there was significant difference between two groups(χ2=6.719, P<0.05). Conclusions The nursing intervention under the guidance of health belief helps colorectal cancer high-risk population establish health belief, enhances participation rates of colonoscopy screening and has important significance to positive rates of early colorectal cancer.

Objective:To explore the depressive symptom of nurses and its relationship with trait coping style,role perceive and personal control.Methods:425 nurses in the 2nd Hangzhou general hospital completed a battery of questionnaires consisted of part items of SCL-90,Trait Coping Style Questionnaire(TCSQ) and Role Perceive and Personal Control Scale.Results:There are significant correlation among the SCL-90 score,the coping styles,and the control feeling and role conflict.Based on depression factor ,factors of negative coping style,control feeling,role conflict and positive coping style were included in stepwise regression equation.Conclusion:Depressive symptom of nurses was related to trait coping style,role perceive and personal control.

Objective:To investigate the relationship among college student’s depression symptoms, problem solving appraisal and perceived stress.Methods:275 college students completed questionnaires, including CES-D, Chinese Perceived Stress Scale (CPSS) and Chinese Problem Solving Inventory (CPSI). Results: Among these students, 52% reported on the level of health risk stress (HRS), 55.3% had depression symptoms. There were significant correlation among depression symptoms, perceived stress and problem solving appraisal. The causal relationship between them were identified with the path analysis method, problem solving in special, seems to have both direct and indirect effect on the prediction of depression and on perceived stress. Conclusion:There is a high level of college student perceived stress and depression symptoms. Problem solving appraisal is an important factor for both of them.

Objective To Analysis the physical fitness participation of 1243 community residents and to provide the scientific basis for developing intervention strategies. Methods The study was based on data of Physical Activity Questionnaire collected in Nutrition and Health Survey 2006 in Putuo, Shanghai. 1 243 subjects aged 6 years and above were involved. Results 43.8% of community residents had participated in physical exercise. Participation ratio was 41.4% in male, lower than that in female (45.8%). Participation ratios among aged 6yr～, 18yr～, 45yr～, 60yr～ were 48.1%, 25.2%, 22.0%, 64.9% in male and 40.8%, 22.5%, 40.1%, 67.1% in female. Participation ratios among age groups were significantly different (P=0.000). Proportion for frequent exercise of all age groups were 25.0%, 11.9%, 19.7%, 64.9% for male and 21.1%, 15.0%, 37.4%, 66.3% for female. There were difference among age groups (P= 0.000). Conclusion Specific intervention measures should be developed for different kind of people.

Objective To analyze the correlation between serum hs -CRP levels and MR Imaging in ischemic cerebrovascu-lar patients.To explore the hs -CRP serum levels in ischemic cerebrovascular patients incidence of early diagnosis and prognosis e-valuation application value.Methods 300 cases of ischemic cerebrovascular disease as the research object were selected from May 2013 to December 2014.The disease is classified into acute cerebral infarction group 100 cases, acute phase of cerebral infarction group 140 cases and transient ischemic attack 60 cases.Among 100 cases of patients with acute cerebral infarction group, in accord-ance with the MR Imaging data, they can be subdivided into three groups.Group A: lacunar infarction group 28 cases; Group B:small area of infarction group 50 cases and group C: large area of infarction group 22 cases.Another 40 healthy physical examination were selected as control group.The hs -CRP serum levels were made correlation analysis with MR Imaging.Results The acute cerebral infarction and transient ischemic attack group serum hs -CRP level difference compared with the control group with statisti-cal significance (p<0.05); The SPSS Spearman correlation analysis result shows that the hs -CRP serum levels and MR Imaging data shown in the infarction area and the body has a direct correlation .Conclusion The ischemic cerebrovascular disease patients se-rum hs-CRP level with MR Imaging have shown increased infarct size and raise.Damage area is bigger, and the corresponding serum hs-CRP levels changes more violently.

Objective: To investigate the targeting and anti-tumor ability of the tumor vessel-specific antibody ScFvH1 selected from phage-ScFv library, and to discuss the application of the antibody in clinical diagnosis and therapy of cancer. Methods: The ScFvH1 gene was inserted into pET-28a(+)/EGFP vector containing green fluorescent protein(GFP) gene and pTIG-Trx vector containing thioredoxin gene; the products were then expressed in E.coli and purified by using Ni-NTA. Tumor-bearing mice model was established by subcutanuous injection of cervical cancer cell line HeLa. The mice were injected with purified ScFv-EGFP fusion protein through vena caudalis and the GFP signals were observed by fluorescent microscope to evaluate the targeting ability of the antibody. Meanwhile, the mice model also received intratumoral injection of purified ScFv-EGFP fusion protein to evaluate the anti-tumor effect of the antibody. Results: Soluble ScFvH1 gene and ScFvH1-EGFP protein were successfully expressed in E.coli; a single band was showed in SDS-PAGE after the purification by Ni-NTA. We found that ScFvH1-EGFP fusion protein was enriched to tumor tissues, but there was only weak fluorescent signal when EGFP protein was injected. No EGFP signal was observed in the lung of tumor-bearing mice. Tumor inhibition experiment showed that the tumor growth in the antibody treatment group was similar to that of the PBS control group. Conclusion: The tumor vessel-specific antibody ScFvH1 selected from phage-ScFv library can specifically target tumor vessels, but it has no obvious inhibitory effect on tumor growth. Our findings pave a way for antibody in cancer diagnosis and treatment.

Objective To investigate the effect of p53-gene expression on renal function in rat with low birth weight ( LBW) .Methods Fifteen pregnant rats were randomly assigned into normal group, LBW group, and L-arginine ( L-Arg)-treated group.For normal group, pregnant rats were fed with 21% protein diet during pregnancy.For LBW and L-Arg treated groups, rats were fed with 10%protein diet.After de-livery, all rats were fed with 21%protein diet.For L-Arg-treated group, rats were given a supplementation with L-Arg (200 mg/kg) drinking water during 21 d lactation, other rats were given running water.At the age of 2m, the ultrastructural change in mesangial cell and podocyte was observed with electron microscope. At the age of 7 d, 21 d, 2 m, and 3 m old, the p53 mRNA expression in renal tissues was observed with re-verse transcription polymerase chain reaction ( RT-PCR) , blood and urine were collected to detect biochem-ical indicators of renal function and 24 h-urine protein.Results ⑴At the age of 3 m,the blood urea nitro-gen ( BUN) and urine cretinine ( UCr) in normal group were significantly lower than those in LBW group ( P <0.01).Compared to normal group, the Ucr of LBW group was significantly lower at the age of 2 m ( P<0.05).At the age of 3 m,the Ucr of L-Arg treated group and LBW group was significantly lower ( P <0.05), the level of 24 h-Urine protein was notably increased in LBW group and L-Arg treated group than that in normal group ( P <0.01, P <0.05) .⑵At the age of 3 m,the expression of p53 mRNA in LBW group was higher obviously than that in normal group.There is a significantly negative correlation between the expression of p53 mRNA and the level of Ucr in LBW group ( r =-0.91, P <0.05).⑶ There were mesangial cell proliferation with matrix increase, filtration membrane podocyte reduction, and partially dis-solved in LBW group, the mesangial cell proliferation of L-Arg treated group was decreased compared to that in LBW group.Conclusions The higher expression of p53 gene in LBW group might be one of reasons for the decreased renal function in LBW rats.

Objective To explore the neural basis of the visual illusory motion using the event-related potential(ERP).Methods One hundred trials(each duration 2 000 ms)of a visual illusory motion figure,rippling wheat pattern made by Akiyoshi Kitaoka,and a visual static figure made of modified rippling wheat pattern were randomly presented with equal probability.Ten healthy right handed undergraduate students as subjects were asked to judge the stimulus whether motional or not.The EEG was recorded from 128 scalp sites using with electrodes mounted in HydroCel GSN cap(Electrical Geodesics Incorporated,Oregon,USA).Results 1)The C1 component of ERP could be evoked by both visual illusory motion pattern and static pattern in POz.The peak of C1 component was presented about 75 ms after each stimulus.C1 component evoked with visual illusory motion was negative,but it's static pattern to be positive.2)P100 and P200 components could be obviously evoked by both kinds of stimuli,but in O2 the amplitudes of P100 evoked by different stimuli were significant different and in T3 the amplitudes of P200 were significant different too.Conclusion The visual illusory motion is formed at the primary visual cortex.It may be related to the organized mode of perception.

To observe the acupuncture treatment of 38 patients with menopausal syndrome. The main acupoints were Fengchi (GB 20), Taiyang (Ex-HN 5), Hegu (LI 4), Neiguan (PC 6), Qihai (CV 6),Yanglingquan (GB 34), Taichong (LR 3) and Taixi (KI 3). The adjunct acupoints were added under differentiation. After one to three therapeutic courses clinical recovery occurred in 26 cases, effectiveness in 10 cases, and ineffectiveness in 2 cases.