Objective To investigate the quality of life of the elderly living at a nursing home and its influencing factors.Methods One hundred and sixty elderly living at one nursing home of Shanghai completed 36-item Short Form (SF-36) Health Survey,Social Support Rate Scale,Geriatric Depression Scale (GDS) and UCLA Loneliness Scale (UCLA-LS) to evaluate the factors that influenced the quality of life and the relationship between quality of life,social support,depression and loneliness in December of 2011.Results The mean age of the elderly was (82.0 ± 8.8).The polynary linear step-wise regression analysis showed that physical function of the elderly was mainly influenced by having children or not,frequency of physical activity and self-assessment of health condition.Role-physical,social function,vitality and role-emotional of the elderly were mainly influenced by age,frequency of physical activity and selfassessment of health condition.Bodily pain of the elderly was mainly influenced by sex and self-assessment of health condition.General health of the elderly was mainly influenced by age,frequency of physical activity,chronic diseases,and self-assessment of health condition.Mental health of the elderly was mainly influenced by age,living status,frequency of physical activity and self-assessment of health condition.Subjective support was positively related to role-emotional and mental health.However,the life quality of the elderly was positively correlated with objective support,utilization of support,total score of social support and each dimension of SF-36.The depression and loneliness of the elderly were negtively related to each dimension of SF-36.Conclusions Multiple factors may influence the quality of life of the elderly.The whole society and family members should make concern and set up better environment to for the elderly.

Objective To discuss the effects of nursing intervention under the guidance of health belief on enteroscopy compliance of colorectal cancer high-risk population. Methods A total of 260 people, who were clearly identified as colorectal cancer high-risk population during preliminary screening of 2 communities in Nankai District, Tianjin but had not finished enteroscopy, were selected and divided them into intervention group and control group with 130 people per group by random digits table method. In the intervention group, subjects received nursing intervention with guidance of the health belief model for a year, while in the control group, subjects simply received regular propaganda and education about colorectal cancer screening. Results After intervention, the cognitive levels in colorectal cancer screening, enteroscopy screening, high-risk factors was (9.16±2.95), (14.55±3.41), (14.25±4.26) points in intervention group and (7.28 ± 3.26), (12.23 ± 3.72), (13.12 ± 4.11) points in control group, and there was significant difference between two groups (t=4.855, 5.228, 2.180, P<0.01 or 0.05). After intervention, the scores of health belief including susceptibility, benefits, barriers, self-efficacy was (7.74 ± 1.50), (13.20 ± 2.45), (6.59 ± 1.91), (23.54±3.16) points in intervention group, and (6.68 ± 1.47), (11.58 ± 2.15), (8.42 ± 1.99), (20.82 ± 3.02) points in control group, and there was significant difference between two groups(t=5.730, 5.682,-7.561, 7.096, P<0.01). The rate of enteroscopy screening was 20.47%(26/127) in intervention group and 8.87%(11/124) in control group, and there was significant difference between two groups(χ2=6.719, P<0.05). Conclusions The nursing intervention under the guidance of health belief helps colorectal cancer high-risk population establish health belief, enhances participation rates of colonoscopy screening and has important significance to positive rates of early colorectal cancer.

Objective:To explore the depressive symptom of nurses and its relationship with trait coping style,role perceive and personal control.Methods:425 nurses in the 2nd Hangzhou general hospital completed a battery of questionnaires consisted of part items of SCL-90,Trait Coping Style Questionnaire(TCSQ) and Role Perceive and Personal Control Scale.Results:There are significant correlation among the SCL-90 score,the coping styles,and the control feeling and role conflict.Based on depression factor ,factors of negative coping style,control feeling,role conflict and positive coping style were included in stepwise regression equation.Conclusion:Depressive symptom of nurses was related to trait coping style,role perceive and personal control.

Objective:To investigate the relationship among college student’s depression symptoms, problem solving appraisal and perceived stress.Methods:275 college students completed questionnaires, including CES-D, Chinese Perceived Stress Scale (CPSS) and Chinese Problem Solving Inventory (CPSI). Results: Among these students, 52% reported on the level of health risk stress (HRS), 55.3% had depression symptoms. There were significant correlation among depression symptoms, perceived stress and problem solving appraisal. The causal relationship between them were identified with the path analysis method, problem solving in special, seems to have both direct and indirect effect on the prediction of depression and on perceived stress. Conclusion:There is a high level of college student perceived stress and depression symptoms. Problem solving appraisal is an important factor for both of them.

Objective To Analysis the physical fitness participation of 1243 community residents and to provide the scientific basis for developing intervention strategies. Methods The study was based on data of Physical Activity Questionnaire collected in Nutrition and Health Survey 2006 in Putuo, Shanghai. 1 243 subjects aged 6 years and above were involved. Results 43.8% of community residents had participated in physical exercise. Participation ratio was 41.4% in male, lower than that in female (45.8%). Participation ratios among aged 6yr～, 18yr～, 45yr～, 60yr～ were 48.1%, 25.2%, 22.0%, 64.9% in male and 40.8%, 22.5%, 40.1%, 67.1% in female. Participation ratios among age groups were significantly different (P=0.000). Proportion for frequent exercise of all age groups were 25.0%, 11.9%, 19.7%, 64.9% for male and 21.1%, 15.0%, 37.4%, 66.3% for female. There were difference among age groups (P= 0.000). Conclusion Specific intervention measures should be developed for different kind of people.

Objective To analyze the correlation between serum hs -CRP levels and MR Imaging in ischemic cerebrovascu-lar patients.To explore the hs -CRP serum levels in ischemic cerebrovascular patients incidence of early diagnosis and prognosis e-valuation application value.Methods 300 cases of ischemic cerebrovascular disease as the research object were selected from May 2013 to December 2014.The disease is classified into acute cerebral infarction group 100 cases, acute phase of cerebral infarction group 140 cases and transient ischemic attack 60 cases.Among 100 cases of patients with acute cerebral infarction group, in accord-ance with the MR Imaging data, they can be subdivided into three groups.Group A: lacunar infarction group 28 cases; Group B:small area of infarction group 50 cases and group C: large area of infarction group 22 cases.Another 40 healthy physical examination were selected as control group.The hs -CRP serum levels were made correlation analysis with MR Imaging.Results The acute cerebral infarction and transient ischemic attack group serum hs -CRP level difference compared with the control group with statisti-cal significance (p<0.05); The SPSS Spearman correlation analysis result shows that the hs -CRP serum levels and MR Imaging data shown in the infarction area and the body has a direct correlation .Conclusion The ischemic cerebrovascular disease patients se-rum hs-CRP level with MR Imaging have shown increased infarct size and raise.Damage area is bigger, and the corresponding serum hs-CRP levels changes more violently.

Objectives To investigate the effect and mechanism of terbutaline sulfate on pulmonary fluid transport in pre-mature rats. Methods Pregnant rats were randomly divided into 5 groups (control group, premature group, low-dose terbutaline group, high-dose terbutaline group and dexamethasone group). Drugs were administered by gavage after rats were fertilized for 16 days and continued for 3 days. Premature rats were taken out from the 19 days pregnant rats, and mature rats were delivered on the due day. Lungs were collected, and the ratio of pulmonary wet weight to dry weight (W/D), Na+, K+-ATP ase activity and concentration of cyclic adenosine monophosphate (cAMP) were measured in lungs. Results The W/D rate, Na+,K+-ATPase acti-vity and cAMP concentration in lungs had significant difference among different groups (P<0.01). The W/D rate was highest in premature group and lowest in the control group. It was lower in the high-dose terbutaline group than in the low-dose terbutaline group and the dexamethasone group (P<0.05). The Na+,K+-ATPase activity was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, and high-dose terbutaline group than in premature group, and it was higher in high-dsoe terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). The cAMP levle was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, high-dose terbutaline group than in premature group, and it was higher in high-dose terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). Conclusions Terbutaline sulfate facilitates lung fluid transport in premature rats, leading to reduce the W/D rate in terbutaline-treated group. We speculate that this effect is related to the increased cAMP level and Na+, K+-ATPase activity in lung tissue.

Pathophysiological changes, such as the vulvovaginal atrophy, reduction in the proportion of lactobacilli, unbalance of the vaginal flora and impaired vaginal immune barrier, can result in a series of symptoms, signs and diseases affecting the health and quality of life in postmenopausal women.This article reviews the change characteristics and related mechanisms of the microbial flora in postmenopausal women in terms of the type, quantity and proportion of vaginal flora, and clarifies efficacy and its mechanisms of systemic and topical estrogen therapy on the improvement of intravaginal environment and urogenital symptoms, in order to improve the quality life in postmenopausal women.

Objective To explore the neural basis of the visual illusory motion using the event-related potential(ERP).Methods One hundred trials(each duration 2 000 ms)of a visual illusory motion figure,rippling wheat pattern made by Akiyoshi Kitaoka,and a visual static figure made of modified rippling wheat pattern were randomly presented with equal probability.Ten healthy right handed undergraduate students as subjects were asked to judge the stimulus whether motional or not.The EEG was recorded from 128 scalp sites using with electrodes mounted in HydroCel GSN cap(Electrical Geodesics Incorporated,Oregon,USA).Results 1)The C1 component of ERP could be evoked by both visual illusory motion pattern and static pattern in POz.The peak of C1 component was presented about 75 ms after each stimulus.C1 component evoked with visual illusory motion was negative,but it's static pattern to be positive.2)P100 and P200 components could be obviously evoked by both kinds of stimuli,but in O2 the amplitudes of P100 evoked by different stimuli were significant different and in T3 the amplitudes of P200 were significant different too.Conclusion The visual illusory motion is formed at the primary visual cortex.It may be related to the organized mode of perception.

Objective: To investigate the targeting and anti-tumor ability of the tumor vessel-specific antibody ScFvH1 selected from phage-ScFv library, and to discuss the application of the antibody in clinical diagnosis and therapy of cancer. Methods: The ScFvH1 gene was inserted into pET-28a(+)/EGFP vector containing green fluorescent protein(GFP) gene and pTIG-Trx vector containing thioredoxin gene; the products were then expressed in E.coli and purified by using Ni-NTA. Tumor-bearing mice model was established by subcutanuous injection of cervical cancer cell line HeLa. The mice were injected with purified ScFv-EGFP fusion protein through vena caudalis and the GFP signals were observed by fluorescent microscope to evaluate the targeting ability of the antibody. Meanwhile, the mice model also received intratumoral injection of purified ScFv-EGFP fusion protein to evaluate the anti-tumor effect of the antibody. Results: Soluble ScFvH1 gene and ScFvH1-EGFP protein were successfully expressed in E.coli; a single band was showed in SDS-PAGE after the purification by Ni-NTA. We found that ScFvH1-EGFP fusion protein was enriched to tumor tissues, but there was only weak fluorescent signal when EGFP protein was injected. No EGFP signal was observed in the lung of tumor-bearing mice. Tumor inhibition experiment showed that the tumor growth in the antibody treatment group was similar to that of the PBS control group. Conclusion: The tumor vessel-specific antibody ScFvH1 selected from phage-ScFv library can specifically target tumor vessels, but it has no obvious inhibitory effect on tumor growth. Our findings pave a way for antibody in cancer diagnosis and treatment.