Objective To evaluate objectively the traditional methods on the assessment of bladder outlet obstruction(BOO) due to BPH. Methods Correlation between the urodynamic findings and the traditional diagnostic parameters such as age,IPSS,Vp,Qmax z and PVRr was studied.The clinical prostatic score(CPS),derived from multiple regression of clinical parameters depending on URA,was evaluated. Results The parameters such as age,IPSS,Vp,Qmax z and PVRr were evaluated with reference to pressure flow study.Every parameter alone was not enough for BOO diagnosis. The regression equation was CPS=49.8-3.3 Qmax z+0.5 IPSS+0.2 Vp+7.5 PVRr.Correlation coefficient between CPS and urodynamic findings was 0.629 and was significantly higher than that of any clinical parameter alone.With CPS≥35,the sensitivity was 83.7% and specificity 85.8% for the diagnosis of BOO.With CPS

Objective To study the efficacy of electroacupuneture combined with auricular acupoint seeds on cluster headache and its adverse reaction interfering.Methods A total of 98 cases with stroke were randomly recruited into a control group of 49 cases and a treatment group Of 49 cases.The control group was treated with conventional medicine,while the treatment group was treated with electro-acupuncture combined with auricular acupoint seeds,besides tge conventional intemal medicine.The efficacy was evaluated after one monm's treatment.A follow-up efficacy was evaluated in the continuous 6 months.Results 2 cases in the treatment group and 1 case in the control group quitted in the process.The treatment group showed significant better results in the immediate efficacy,3-mortth follow-up efficacy and 6-months follow-up efficacy as compared to the control group(all P＜0.05).The adverse reaction incidences of the treatment group were also significantly lower than the control grouD at 3-months and 6-months follow-up study(all P＜0.05).Conclusion The therapy of electro-acupuncture combined with auricular acupoint seeds is helpful to improve pharmacotherapy effect and alleviate medium term and long term adverse drug reaction.It can be served as an effective preventative measure for cluster headache.

OBJECTIVE To investigate the ?-lactamase genes and virulence gene in Escherichia coli isolated from the old patients.METHODS Twenty strains of E. coli were selected to test 15 kinds of ?-lactamase genes (TEM,SHV,CTX-M-1 group,CTX-M-2 group,CTX-M-9 group,OXA-1 group,OXA-2 group,OXA-10 group,PER,GES,VEB/CEF,CARB,DHA,ACT/MIR and LAT/CMY) and two virulence genes (CNF-1 and CNF-2).RESULTS Six ?-lactamase genes and one virulence gene have been found out from these 20 strains of E. coli. The results showed that there were 14,3,1,5,2,2 and 1 strain which carried TEM,SHV,CTX-M-1 group,OXA-1 group,DHA,ACT/MIR and CNF-1,respectively. These 20 E. coli strains contained at least 1 ?-lactamase gene and 7 E. coli strains were found containing more than 2 kinds of ?-lactamase at the same time.CONCLUSIONS The resistance of these 20 E. coli strains to ?-lactam antibiotics is closely relative to ?-lactamase.

Objective To observe the preventive effects of electroacupuncture therapy on glenohumeral subluxation(GHS)in patients of cerebral stroke of super-early period. Methods A total of 160 cases with cerebral stroke were randomly recruited into a control group of 80 cases and a treatment group of 80 cases. The control group was treated with conventional medicine and rehabilitation training, while the treatment group was given electro-acupuncture therapy based on conventional medicine and rehabilitation training. All treatments were performed once in each day, and one treatment course lasted for ten days. The preventive effect was evaluated after the successive three courses. Results 4 cases in treatment group and 2 cases in control group quitted in therapeutic process. The GHS incidence rate in the third course of treatment group was significantly lower than control group (P<0.05). The severe GHS incidence rate of treatment group was significantly lower than control group in the second course (P<0.05)and the third course (P<0.01). The GHS scale of the treatment group showed no significant difference in all three courses (P>0.05), while the control group showed significant difference in one course of all three(P<0.05). Conclusion Electro-acupuncture therapy can significantly decrease GHS incidence rate, relieve pathogenetic condition, control its progress, and so thus prevent GHS of stroke patients in super-early period.

Objective To investigate the effects of 17?-estradiol (E2) on the gene expression of typeⅠA bone morphogenetic protein receptor (BMPR-ⅠA) and core-binding factor alpha 1 (Cbf?1) in rat bone marrow stromal cells exposed to the differentiation medium and to elucidate the effects of E2 on osteoblastogenesis. Methods Adherent bone marrow stromal cells were cultured in differentiation medium containing DEX (10 -7 mol/L), 1,25-(OH)2D3 (10 -9 mol/L) and different concentrations of E2. Effects of different concentrations of E2 on the gene expression of BMPR-ⅠA and Cbf?1 was quantified by RT-PCR based on the comparison with an internal reference, ?-actin expression, and identified by Northern blotting. Alkaline phosphatase (ALP) activity of cells was detected. Contents of type Ⅰ collagen were determined by Van Gieson staining. Results E2 could evidently inhibit the expression of BMPR-ⅠA and Cbf?1 mRNA during the differentiation process of bone marrow stromal cells into osteoblasts in a dose-dependent manner. These were confirmed by Northern blotting. The ALP activity increased in a concentration-dependent manner, but the amount of type Ⅰ collagen decreased in a concentration-dependent manner. Conclusion E2 can significantly inhibit the gene expression of BMPR-ⅠA and Cbf?1 in bone marrow stromal cells and inhibit osteoblastogenesis in vitro.

[ABSTRACT]AIM:Toinvestigatetheeffectofsilencingisocitratedehydrogenase2(IDH-2)genebysmallinter-fering RNA (siRNA) on the biological characteristics of human small cell lung cancer cell line NCI -H446.METHODS:IDH-2 expression was knocked down in human small cell lung cancer cell line NCI -H446 by siRNA-IDH-2.The expression level of IDH-2 was determined by real-time PCR and Western blotting .The cell proliferation was measured by CCK-8 as-say , the protein expression of MAPK p 42 was detected by Western blotting , and the cell cycle was analyzed by flow cytome-try.The migration was observed using Transwell cell migration system .BALB/c nude mice were subcutaneously injected on the back with NCI-H446 cells transfected with siRNA-IDH-2/negative control siRNA or non-transfected cells to study the tumor growth .RESULTS:siRNA-IDH-2 remarkably down-regulated the expression of IDH-2 and MAPK p42 in the NCI-H446 cells.siRNA-IDH-2 inhibited both the proliferation and migration abilities of NCI-H446 cells, and the cell cycle was arrested in S phase as compared with negative control group .Additionally, the volume of xenograft tumors in siRNA-IDH-2 group was significantly decreased as compared with control group .CONCLUSION:siRNA-IDH-2 down-regulates the expres-sion of IDH-2 in NCI-H446 cells, reduces the cell migration efficiency and inhibits the tumor growth in vitro and in vivo.

AIM:To investigate the effects of 17?-estradiol (E_2) on the gene expression of typeⅠA and typeⅠB bone morphogenetic protein receptor (BMPR-ⅠA,ⅠB) in rat bone marrow stromal cells exposured to the differentiation medium and to elucidate the effects of E_2 on osteoblastogenesis. METHODS: Adherent bone marrow stromal cells were cultured in differentiation medium containing DEX(10 -7 mol?L -1 ) and 1,25(OH)_2D_3 (10 -9 mol?L -1 ) and different concentrations of E_2. The gene expression of BMPR-ⅠA,ⅠB was quantified by semiquantitative RT-PCR. RESULTS: E_2 evidently inhibited the expression of BMPR-ⅠA mRNA in bone marrow stromal cells.The suppression was dose-dependent. When examined under various concentrations of E_2 (0-10 -6 mol?L -1 ),the expression of BMPR-ⅠA mRNA were decreased from (25.7?2.5)% to(16.3?1.5)%( P

Parkinson's disease (PD) is the second most common neurodegenerative disorder of the central nervous system, affecting 1 in 50 people over the age of 60 years. PD is the result of loss of a majority of dopamine (DA) neurons in the midbrain substantia nigra. The basic pathological feature of PD is the formation of intracytoplasmic eosinophilic inclusion bodies, Lewy bodies. So far, what leads to DA neuron degeneration is uncertain. Thirteen familial PD related loci have been identified, including six mutations:α-SYN(PARK1/4), Parkin(PARK2), UCHL1(PARK5), PINK1(PARK6), DJ-1(PARK7) and LRRK2 (PARK8). Drosophila has been widely used in the study of human diseases because of its genetic advantages. The Drosophila DA synthesis is similar to human, and Drosophila DA system is also involved in motion control, so it is generally considered that DA neuron death of Drosophila can be a perfect model of PD. In this article we review the progress of research methods based on Drosophila model in study of mechanisms related to Parkinson's disease.
Animals ; Disease Models, Animal ; Drosophila ; genetics ; Humans ; Mutation ; Parkinson Disease

Objective To evaluate the serum pyridinoline cross-linked carboxyteminal telopeptide of type Ⅰ collagen ( ICTP) in the early diagnosis potency,efficacy and prognosis of tumor bone metastasis. Methods According to emission computed tomography(ECT) ,MRI and X-ray results,336 cases of tumor were divided into higher ICTP (5. 98 ± 1. 95μg/L ) than normal values. Twenty-two cases were identified bone metastasis through PET/CT examination. 26 cases were identified bone the effective cases decreased from( 13. 22 ± 4.65)μg/L (before treatment) to (7. 18 ±3. 54)μg/L (after treatment) (t = 10. 076,P = 0. 000). Conclusions Serum ICTP is helpful for the early diagnosis, screening and efficacy evaluation of tumor bone metastasis. It could be used for monitoring the occurrence of tumor bone metastasis and its prognosis.

In the present study, the apoptotic mechanism and polyamine transporter recognition of WJH-6, a novel polyamine conjugate, were investigated in K562 and HL-60 cells. The cytotoxicity of WJH-6 was assessed by MTT assay; cell cycle distribution and apoptosis were measured by flow cytometry; the protein expression of Caspase-3, Caspase-8, Caspase-9, Bid and mitochondrial membrane potential (MMP) were evaluated by high content screening (HCS) analysis; the protein expression of cytochrome c was measured by Western blotting. The results showed that WJH-6 could be recognized and transported by polyamine transporter (PAT). Furthermore, WJH-6 was able to inhibit K562 and HL-60 cells proliferation and induce apoptosis. This apoptotic effect was relative to MMP loss, cytochrome c release from mitochondria to cytoplasm and the activation of Caspase-8, Caspase-9, Caspase-3 and Bid. These results suggested that WJH-6-induced K562 and HL-60 cells apoptosis was related with mitochondrial damage.