1.Efficacy analysis of Q-switched alexandrite laser in the therapy of cafe-au-lait spots
Chinese Journal of Dermatology 2009;42(9):610-612
5). Conclusions Q-switched alexandrite laser is an effective approach to the treatment of care -au-lait spots, and its efficacy can be predicted according to different factors that concern.
2.Content Determination of Aspirin: Comparison among 3 Calculation Methods
China Pharmacy 1991;0(06):-
OBJECTIVE: To compare 3 different calculation methods in the determination of aspirin in the bicomponent (aspirin and salicylic acid) system. METHODS: Different concentrations of mixed standard solution of aspirin and salicylic acid were prepared and scanned under a wavelength ranged from 230nm to 350nm. The absorbabilities of the 2 constituents were recorded at 265nm, 295nm and 320.5nm, and the data were treated with Iso-absorption diplo-wavelength method, proximal multiplying factor coefficient method and SA concentration fractional approximate calculation method. The recoveries of aspirin were calculated. RESULTS:The recoveries of aspirin determined by 3 different methods were (99.11?1.68)%, (98.82?1.17) % and (96.23?4.10)%, respectively. CONCLUSION: The proximal multiplying factor coefficient method is stable with little fluctuation in determination results, which proved to be simple while effective for the determination of sample at a great quantity.
3.Effects of endothelin antagonist on melanogenesis of cultured B16 murine melanoma cells
Pinru WU ; Xiangdong CHEN ; Hui XU ; Beiqing WANG ; Jianhang LIU
Chinese Journal of Dermatology 2009;42(2):108-111
Objective To evaluate the biological effect of endothelin (ET) antagonist on cultured B16 murine melanoma cells. Methods B16 murine melanoma cells were cultured in the presence of various concentrations (31.25, 62.5, 125, 250, 500 μg/mL) of ET antagonist or licoflavone. Then, melanoma cells were harvested for the detection of tyrosinase activity and melanin content. The proliferation rate of melanoma cells was measured with MTT method. The effect of ET antagonist was compared with that of licoflavone. Results Licoflavone had a concentration-dependent inhibition on melanogenesis. The ET antagonist selectively suppressed the ET-induced stimulation of tyrosinase and cell differentiation of B16 cells, but had no direct inhibitory effect on melanogenesis in culture, and little influence on melanocyte viability. The addition of ET antagonist at 200 μg/mL could significantly inhibit ET (0.5 μg/mL)-induced melanogenesis in Bl6 cells. The cytotoxity of the antagonist was relatively lower than that of licoflavone. Conclusions The results suggest that the ET antagonist is a safe skin-whitening ingredient, and may have a wide application perspective in the prevention of endothelin-induced skin pigmentation after UVB irradiation.
4.Effects of laser, radiofrequency and intensive pulse light on injectable polymethylmethacrylate ( Artecoll)
Ye FEI ; Xiangdong CHEN ; Beiqing WANG ; Jingan CHEN ; Zhengyu SHEN ; Hui XU ; Jianhang LIU ; Zhen ZHANG
Chinese Journal of Medical Aesthetics and Cosmetology 2011;17(1):37-40
Objective To evaluate the safety and efficacy of polymethylmethacrylate/PMMA (Artecoll) as an injectable dermal filler after using laser, radiofrequency and intensive pulse light. Methods In vitro, factional photothermolysis (Affirm) was directly used on the PMMA to see if there was any change in the structure or surface smoothness. In vivo, pig and guinea pig were used as the short-term and long-term models, respectively, for Artecoll. The dermal filler was first injected and then laser treatments conducted.The skin sample was observed through light microscope, scanning electronic microscope and transmission electronic microscope to see whether there were any structural changes, infiltration of inflammatory cells and the regeneration of collagen. Results Through the microscopic observation of in vitro experiment and the animal models, no distortion or rupture of the microsphere were found after the laser treatment. No uneven surface was found in the PMMA microsphere either. The microshpere was surrounded by the inflammatory cells. The infiltration was mild to intermediate with few foreign body giant cells even after several treatments of laser. There was no macrophage seen. But the collagen regeneration was distinguishable after the laser treatment. No obvious structural change was found eventually. Conclusion Artecoll is a safe and effective permanent injectable dermal filler for wrinkle reduction and contour refining.
5.Study on the electrochemical immunosensor for ultrasensitive detection of myeloperoxidase
Lingsong LU ; Bei LIU ; Xiao MA ; Cheng YU ; Shun WU ; Jianhang LENG
Chongqing Medicine 2015;(36):5109-5111
Objective To construct an electrochemical immunosensor for ultrasensitive determination of myeloperoxidase (MPO) .Methods The electrochemical immunosensor for M PO was prepared by modifying the electrode using Au-graphitized me-soporous carbon nanoparticles(AuNPs@ GMCs) hybrid and immobilizing MPO antibodies onto the glass carbon electrode surface . The effect of experimental parameters on the immunosensor and results comparison with ELISA were investigated .Results The immunosensor was sensitive to M PO with a linear relationship between 2 .000 and 300 .000 ng/mL and a correlation coefficient of 0 .999 ;the detection limit was 0 .5 ng/mL .The correlation coefficient of two methods was 0 .983 .Conclusion The immunosensor can be used for ultrasensitive detection of MPO .
6.Clinical efficacy of fractional laser (AffirmTM) with combined apex pulse and multiplex technology on post-acne scars and other skin lesions
Ye FEI ; Jinan CHEN ; Xiangdong CHEN ; Zhengyu SHEN ; Jianhang LIU ; Beiqing WANG ; Hui XU ; Zhen ZHANG
Chinese Journal of Medical Aesthetics and Cosmetology 2012;18(1):34-36
Objective To evaluate the clinical efficacy of factional multiplex laser (AffirmTM) with combined apex pulse (CAP) technology in treating depressive acne scars,post inflammatory erythema and enlarged pores and other lesions.By following up,the correlative factors were analyzed to guide the further use of the factional laser (AffirmTM) with CAP technology.Methods Patients who received the AffirmTM laser therapy from Sept.2007 to July 2008 were enrolled in this retrospective follow-up study.Pictures were taken before and after each treatment during the therapy.Then the pictures were evaluated by dermatologists to attain object variables.Other subject variables from patients were recorded during survey.The relation between the efficacy and age,sex and treatment frequencies were analyzed statistically.Results The effective rate was 25.34 % for post-acne scars,36.84 % for post-acne erythema.28.57 % for enlarged pores,and 14.29 % for rythids.Nonparametric test showed no statistical difference with gender or age.There was correlation between efficacy and treatment frequencies.No severe adverse effect was observed.Conclusions AffirmTM laser with CAP technology has a good effect on treating post-acne depressed scars and erythema,enlarged pores and fine rythids.
7.Efficacy of photodynamic therapy on rosacea
Xiaojin WU ; Xiangdong CHEN ; Ye FEI ; Beiqing WANG ; Hui XU ; Jianhang LIU ; Jin'an CHEN
Chinese Journal of Medical Aesthetics and Cosmetology 2014;20(1):36-39
Objective To investigate the efficacy and safety of photodynamic therapy (PDT) in the treatment of different types of rosacea,and to evaluate its benefit by comparing its efficacy with 10% sodium sulfacetamide and 5% sulfur emollient foam.Methods Forty-three subjects with rosacea were enrolled in this study.They were randomized to be treated with PDT (twenty-three subjects) or 10% sodium sulfacetamide and 5% sulfur emollient foam (twenty subjects).PDT group subjects received PDT once every seven to ten days for three times while the 10% sodium sulfacetamide or 5%sulfur emollient foam was applied 2 times per day for thirty days in the other group.Digital photographs were taken before and after one month of treatment in both groups.Blinded independent physicians graded improvement based on these photographs utilizing a percentile evaluation scale.A more than sixty percent remission proved the treatment to be effective.All the complications occurred during the therapy were taken notes and their severity was classified by the subjects.Two dermatology life quality index forms were completed before and after treatment by the subjects to evaluate the change in terms of their quality of life.Results After one month of treatment,PDT proved to be effective on sixty-five percent of subjects (seventy-one percent on papulopustular rosacea and fifty percent on erythematotelangiectatic rosacea).The emollient foam proved to be effective on thirty-five percent of subjects.Statistically significant difference was observed between the overall effective rate of PDT and emollient foam (P<0.05).In addition,a greater improvement in terms of the quality of life was experienced by the PDT group subjects.No irreversible adverse event was observed in both groups during the study.Conclusions PDT proves to be an effective and safe treatment for rosacea with satisfactory efficacy,significant improvement in patients' quality of life and few irreversible side effects.
8.Surgical management and mapping epilepsy in supratentorial tumor
Xinjun WANG ; Ziqiang XU ; Qiao SHAN ; Jixin SHOU ; Peidong LI ; Quan LIU ; Jianhang WU ; Xudong FU ; Lin MA
Clinical Medicine of China 2008;24(6):604-606
Objective To study the value and effectiveness of surgical management and mapping in supratentorial tumoral complicated with epilepsy and to study the correlations between tumor and the epileptogenic focus.Methods The clinical data of 121 patients with supratentorial cerebral tumor but epilepsy as initial symptom were retrospectively analyzed for the incidence of pre-and postoperative epileptic seizures,including grade Ⅰ glioma in 1 5 cases and grade Ⅱ glioma in 35 cases,grade Ⅲ-Ⅳglioma in 12 cases,menigoma in 32 cases,metastases in 10 cases,cavernous angiomas in 15 cases,and ependymomas in 2 cases.Results Surgery based on CT/MRI,seizure type and EEG changes was conducted.There was no death in operation.The highest incidence was in frontal lobe and the lowest in occipital lobe.Correlations between localization of tumor and the epileptogenic focus:there were 50 cases in the same location,near or beside tumors in 28 cases,far separate apart(>2 cm)from tumors in 25 cases,no relationship was found in 18 cases.103 patients were followed up for one to nine years.31 patients had a few seizures in the early postoperative period.Epileptic seizures were cured without anti-epilepsy drugs in 83 cases.Conclusion There are some differences between tumors'location and epileptogenic focus in supratentorial tumoral epilepsy.The location and size of epileptogenic zone should be detected before and during operation.The resection of the tumor combined with the resection of the epileptogenic zone"epilepsy surgery"can provide good results.
9.Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor.
Yinghui WEI ; Meiling ZHANG ; Jing HU ; Yingsi ZHOU ; Mingxing XUE ; Jianhang YIN ; Yuanhua LIU ; Hu FENG ; Ling ZHOU ; Zhifang LI ; Dongshuang WANG ; Zhiguo ZHANG ; Yin ZHOU ; Hongbin LIU ; Ning YAO ; Erwei ZUO ; Jiazhi HU ; Yanzhi DU ; Wen LI ; Chunlong XU ; Hui YANG
Protein & Cell 2023;14(6):416-432
Approximately 140 million people worldwide are homozygous carriers of APOE4 (ε4), a strong genetic risk factor for late onset familial and sporadic Alzheimer's disease (AD), 91% of whom will develop AD at earlier age than heterozygous carriers and noncarriers. Susceptibility to AD could be reduced by targeted editing of APOE4, but a technical basis for controlling the off-target effects of base editors is necessary to develop low-risk personalized gene therapies. Here, we first screened eight cytosine base editor variants at four injection stages (from 1- to 8-cell stage), and found that FNLS-YE1 variant in 8-cell embryos achieved the comparable base conversion rate (up to 100%) with the lowest bystander effects. In particular, 80% of AD-susceptible ε4 allele copies were converted to the AD-neutral ε3 allele in human ε4-carrying embryos. Stringent control measures combined with targeted deep sequencing, whole genome sequencing, and RNA sequencing showed no DNA or RNA off-target events in FNLS-YE1-treated human embryos or their derived stem cells. Furthermore, base editing with FNLS-YE1 showed no effects on embryo development to the blastocyst stage. Finally, we also demonstrated FNLS-YE1 could introduce known protective variants in human embryos to potentially reduce human susceptivity to systemic lupus erythematosus and familial hypercholesterolemia. Our study therefore suggests that base editing with FNLS-YE1 can efficiently and safely introduce known preventive variants in 8-cell human embryos, a potential approach for reducing human susceptibility to AD or other genetic diseases.
Humans
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Apolipoprotein E4/genetics*
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Cytosine
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Mutation
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Blastocyst
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Heterozygote
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Gene Editing
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CRISPR-Cas Systems