Objective To explore the influence of recombinant human brain natriuretic peptide(rhBNP)on the ventricular re-modeling and cardiac function of chronic heart failure rats.Methods Doxorubicin was injected for 2 weeks to obtain the chronic heart failure rats.Then different doses of rhBNP were injected for 4 weeks and objective indexes were recorded.Results The inde-xes were generally improved in the rats with rhBNP injection.The left ventricular absolute weight and left ventricular mass index obviously decreased in both middle and high dose group,but none difference was found between the two groups(P >0.05).The in-dexes of cardiac function of the high dose rhBNP group were improved most obviously,with the systolic pressure,diastolic pres-sure,left ventricular systolic pressure increasing and left ventricular end-diastolic pressure decreasing.right ventricular and diastolic stage decreased significenfly(P <0.05).The serum and myocardial levels of TNF-α/IL-10 generally decreased/increased in the rats after rhBNP treatment(P <0.05).Conclusion Long-term rhBNP treatment is likely to protect the ventricular remodeling and car-diac function and of chronic heart failure,which maybe relative to the positive regulation on inflammatory cytokine network of rhB-NP.

In order to investigate the neurotoxicity of lipopolysaccharide (LPS) on the dopaminergic neurons of substantia nigra and the pathogenesis of Parkinson disease, LPS was stereotaxically infused into substantia nigra (SN). At different dosages and different time points with 5 microg LPS, the damage of the dopaminergic neurons in SN was observed by using tyrosine-hydroxylase (TH) immunohistochemical staining. The results showed that 14 days after injection of 0.1 microg to 10 microg LPS into the rat SN, TH-positive (TH+) neurons in the SN were decreased by 5%, 15%, 20%, 45 %, 96% and 99% respectively. After injection of 5 microg LPS, as compared with the control groups, TH+ neurons began to decrease at 3rd day and obviously decrease at 14th day, only 5% of total cells, and almost disappeared 30 days later. The results suggested that LPS could induce the degeneration of dopaminergic neurons in the SN in a dose- and time-dependent manner.
Dopamine/metabolism ; Dose-Response Relationship, Drug ; Lipopolysaccharides/*toxicity ; *Nerve Degeneration ; Neurons/pathology ; Parkinson Disease, Secondary/*chemically induced ; Random Allocation ; Rats, Sprague-Dawley ; Substantia Nigra/*pathology

In order to investigate the neurotoxicity of lipopolysaccharide (LPS) on the dopaminergic neurons of substantia nigra and the pathogenesis of Parkinson disease, LPS was stereotaxically infused into substantia nigra (SN). At different dosages and different time points with 5 microg LPS, the damage of the dopaminergic neurons in SN was observed by using tyrosine-hydroxylase (TH) immunohistochemical staining. The results showed that 14 days after injection of 0.1 microg to 10 microg LPS into the rat SN, TH-positive (TH+) neurons in the SN were decreased by 5%, 15%, 20%, 45 %, 96% and 99% respectively. After injection of 5 microg LPS, as compared with the control groups, TH+ neurons began to decrease at 3rd day and obviously decrease at 14th day, only 5% of total cells, and almost disappeared 30 days later. The results suggested that LPS could induce the degeneration of dopaminergic neurons in the SN in a dose- and time-dependent manner.
Animals ; Dopamine ; metabolism ; Dose-Response Relationship, Drug ; Female ; Lipopolysaccharides ; toxicity ; Nerve Degeneration ; Neurons ; pathology ; Parkinson Disease, Secondary ; chemically induced ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Substantia Nigra ; pathology

OBJECTIVETo investigate the effects of modified vacuum sealing drainage (VSD) technique in treating refractory sinus in the lower leg.

METHODSFrom January 2010 to December 2011, 11 patients with refractory sinus in the lower leg, including 7 males and 4 females, with an average age of 34.5 years (ranged,23 to 56). These patients were treated with modified VSD technique after thorough cleaning sinus and continuous washing. After 14 to 21 days of treatment, removed VSD and re-debrided the sinus and sutured wound.

RESULTSInfections got control after operation, sinus in the lower leg healed. All patients were followed up from 6 to 14 months with an average of 10 months, no recidivations were found.

CONCLUSIONModified VSD technique is an effective method in the treatment of stubborn sinus in the lower leg.

Adult ; Drainage ; methods ; Female ; Fracture Fixation, Internal ; adverse effects ; Humans ; Leg Bones ; injuries ; surgery ; Male ; Middle Aged ; Postoperative Complications ; therapy ; Vacuum