Objective: To study the saponins from Reineckia carnea of Guizhou Miao medicine. Methods: The chemical constituents from 95% EtOH extract were isolated and purified by repeated silica gel column chromatography, macroporous resin, ODS C18, Sephadex LH-20 gel column chromatography, and Pre-HPLC, and their structures were identified on the basis of physical and chemical properties, HR-ESI-MS and NMR spectral analysis. Results: Ten compounds were isolated and identified as (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol1,16-di (β-D-glucopyranosede) (1), (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol-1-[O-α-L-rhamno-pyranosyl-(1→2)-β-D-glucopyranoside]-16-(β-D-glucopyranoside) (2), (20S,22R)-spirost-25(27)-en-1β,3β,4β,5β-tetraol-5-O-β-D-glucopyranoside (3), kitigenin 5-O-β-D-glucopyrannoside (4), aspidistrin A (5), (17,20-S-trans)-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-(1→2)-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside (6), diosgenin-3-O-β-D-glucopyranoside (7), ophiopogonin T (8), prosaikogenin D (9), and saikosaponin b2 (10). Conclusion: Compounds 3, 5, 8, 9, and 10 are isolated from this plant for the first time, which provided some reference for further elucidation and development of saponins from R. carnea.

To study the kinetic characteristics of moisture adsorption-desorption characteristics of honeysuckle spray powder by dynamic vapour sorption method. Methods: The moisture adsorption and desorption data of honeysuckle spray powder were determined by dynamic vapour sorption instrument under the conditions of temperature of 20, 30, and 40 ℃ and relative humidity of 0-85%. Based on the six kinds of moisture absorption models commonly used, the experimental data were fitted and evaluated. Results: The higher the temperature, the shorter the time required to reach the moisture balance. When the relative humidity was 65%-85%, the quality of honeysuckle powder was obviously changed. The greater the relative humidity, the greater the quality change when the equilibrium was reached. Peleg model was the best mathematical model to describe the moisture adsorption-desorption characteristics of honeysuckle spray powder. Conclusion: The dynamic vapour sorption method can shorten the test time and reduce the human error. It is suitable for the study of moisture absorption characteristics of honeysuckle spray powder.

OBJECTIVE: To explore the effect of positive-pressure ventilation on dust removal in whole lung lavage( WLL).METHODS: By random sample method,21 patients with stage Ⅱ or Ⅲ pneumoconiosis were chosen for different WLL.Using the patients' own left and right lung was used for matched control study. The positive pressure ventilation was performed at the end of the 3rd,6th,9th and 12 th lavage in treatment lung( treatment groups). The positive-pressure ventilation was not implemented at the end of the 3rd and 6th lavage in the contralateral lung( control groups) but implemented at the end of the 9th,11 th and 12 th lavage. The recovery of lavage fluid,dust and dust concentration drained from 4th to 9th lavage were compared in the two groups. RESULTS: There was no statistical difference in the recovery of the lavage fluid in the 4th to 9th lavage in the two groups( P > 0. 05). The amount of dust and the dust concentration in the fourth lavage drainage in the treatment group was higher than that in the control group( P < 0. 01). The amount of dust and the dust concentration in the 6th,8th and 9th lavage drainage in the treatment group was lower than that in control group( P < 0. 01). The amount of dust and the dust concentration in the 3rd positive pressure ventilation were higher than that in the 6th positive pressure ventilation in the treatment group( P < 0. 01). The total amount of dust in the treatment group was higher than that in the the control group( P < 0. 01). CONCLUSION: In whole lung lavage,the positive pressure ventilation can accelerate the discharge of dust in the lung of patients with pneumoconiosis.

OBJECTIVE: To analyze the epidemiological characteristics of new occupational pneumoconiosis(hereinafter referred to as pneumoconiosis) in Jiangxi Province from 2010 to 2019. METHODS: The data of new pneumoconiosis cases in Jiangxi Province from 2010 to 2019 were collected through the Occupational Disease and Occupational Disease Health Information Monitoring Subsystem in the China Disease Prevention and Control Information System, and a retrospective analysis was conducted. RESULTS: From 2010 to 2019, there were 4 450 new cases of pneumoconiosis in Jiangxi Province. Among these cases, the main disease types were coal workers′ pneumoconiosis and silicosis, accounting for 98.5%. The number of new cases of pneumoconiosis showed a decreasing trend with the increase of years for the last ten years(P<0.05). The median age and the 0 th-100 th percentile [M(P_0-P_(100))] of new-onset pneumoconiosis diagnosis was 56.6(34.0-97.0) years old. The service length M(P_0-P_(100)) with dust-exposure was 12.0(1.0-48.0) years. The main industry of the new pneumoconiosis cases was concentrated in the coal mining and washing industry(63.4%). The distribution of economic types of enterprises with new pneumoconiosis cases was mainly state-owned enterprises(70.0%). The enterprise scale was medium-sized and small enterprises(65.9% in total). The types of work were mainly coal blenders, rock drillers, and coal miners, accounting for 56.5%. CONCLUSION: The incidence of occupational pneumoconiosis in Jiangxi Province is not optimistic. It is necessary to strengthen the prevention and control of occupational diseases on workers exposed to dust in key industries, enterprises and types of work.

In recent years, people have paid more attention to the spermatogonial stem cells that have the capacity for self renewal and multilineage differentiation and produce daughter cells that can expand and differentiate into spermatozoa under the adjustment of self genes and external signal. This article reviews recent advances in studies of enrichment and original selection of the spermatogonial stem cells. This review also summarizes some control factors in proliferation and transplantation techniques.
Animals ; Cell Proliferation ; Humans ; Male ; Rats ; Spermatogonia ; cytology ; Stem Cell Transplantation ; Stem Cells ; cytology

OBJECTIVE: To analyze the current status of silica dust hazard in workplaces of Jiangxi Province. METHODS: The level of silica dust in the air of workplaces, occupational health examination of the dust-exposed workers and diagnosis of occupational disease in 1 557 enterprises in Jiangxi Province were investigated and analyzed. RESULTS: The 1 557 enterprises were included in eight industries, and the main types of enterprises were non-coal mining, ceramic products manufacturing and stone processing industry(a total of 85.16%). There were 4 029 jobs/positions that were involved in exposure to silica dust in these workplaces. Among them, the exposure concentration of time weighted average(C_(TWA)) of total dust and respirable dust exceeded 21.52% and 20.55%, respectively. The posts where the total dust and respirable dust that exceed the standard were found in the quartz sand processing industry, non-ferrous metal smelting industry and stone processing industry, that were the top three among the eight industries(all P<0.01). Among the 828 posts with C_(TWA) of respiratory dust exceeding the standard, the C_(TWA) exceeding rate of mild-, moderate-and high-hazardous jobs were 3.87%(156/4 029), 1.66%(67/4 029) and 15.02%(605/4 029), respectively. Among 23 643 dust-exposed workers, the detection rates of occupational contraindications and suspected silicosis were 2.06% and 1.51%, and the prevalence of silicosis was 1.21%. CONCLUSION: Silica dust hazard in workplaces of quartz sand processing industry, non-ferrous metal smelting industry and stone processing industry are serious in Jiangxi Province. The supervision and management of occupational health should be further strengthened to guarantee the occupational health of workers.

Objective　To study the MRI diagnosis of spinal metastasis.Methods　The MRI appearances of 128 cases of spinal metastasis proved clinically were retrospectively analyzed.Results　(1)The MRI signal was T1WI low in 87.5% and 12.5% was mixed or equal.(2)Multi-focus in 80.5%.The "jump up sign"occurred in 52.3%,the accessory parts destructed in 64.1%,in which root of vertebral destructed with expanded in 57%.(3)The masses of paravertebral occurred in 31.3%.The disc of vertebral was not destructed.(4)The patients associated with vertebral compresion fracture such as "wedge","disc"or"wedge of rear"in 52.3%.Conclusion　The MRI findings of spinal metastasis is of high sensitivity and spectivity.The method is advanced than X-ray and CT.

Objective To investigate MRI characteristics of recurrent prostate cancer after endocrinotherapy.Methods Recurrent prostate cancer after endocrinotherapy was diagnosed clinically in 10 cases.MRI examinations were performed before and after recurrence of tumors.T_2 signal intensity and morphological changes of the prostate,involvement of pelvic tissues,metastasis of bone and lymph metastasiswere analyzed.Results The prostates enlarged in 7 patients after recurrence and maintained the same size in the other three.The pelvictissues,including seminal vesicle,bladder,rectal and obturator internus,were involved in 7 patients after recurrence.Metastasis of bone and lymph node progressed in 7 and 9 patients,respectively,but T_2 signal differences were unremarkable between recurrent regions and otherregions.Conclusion MRI examination can provide sufficient radiologic information in the diagnosis of recurrence of prostate cancer afterendocrinotherapy.

50 years old).DWI was done on a 1.5-T system using single-shot diffusion-weighted echo-planar imaging(b-values = 0,300,500 and 800 s/mm~2).ADC maps were calculated using the manufacturer's software.ROIs placed on peripheral zone contained,average,thirty pixels.Results When b-value was 300 s/mm~2,the ADC of peripheral zone in junior,middle-age and senior group were(2.03?0.29)?10~(-3) mm~2/s,(2.37?0.33)?10~(-3) mm~2/s,(2.75?0.29)?10~(-3) mm~2/s,respectively,and statistically significant different(F=36.66,P

Objective To quantitatively analyze the ADC values of different types of benign prostatic hyperplasia(BPH) comparatively with ADC values of prostatic carcinoma(Pca).Methods Seventeen cases of BPH(mean age 69.1 years?4.3 years,ranged from 62 to 77 years) underwent transurethral resection of prostate(TURP) were scanned with the sequence of DWI.DWI(b-values = 0 s/mm2and 800 s/mm2) was performed at 1.5-T system.The hyperplasia tissues resected from central gland were divided into two types pathologically: glandular BPH and stromal BPH.According to the pathological results,ROIs were placed on central gland and the ADC values of two types of BPH were measured.In addition,fifteen patients with biopsy-proved Pca were evaluated and the ADC values of Pca regions were measured.Results The mean ADC values of glandular BPH,stromal BPH and Pca were(1.80?0.20)?10-3 mm2/s,(1.56?0.18)?10-3 mm2/s and(1.27?0.21)?10-3 mm2/s,respectively.Statistically significant difference(F=47.366,P