Objective To observe the therapeutic efficacy and security of hyperthermia combined with chem-otherapy for advanced gastric carcinoma.Methods The clinical data of 126 patients with advanced gastric cancer were retrospectively analyzed.According to the different treatment methods,they were divided into simple chemotherapy group(A group,63 cases)and chemotherapy combined with hyperthermia group (B group,63 cases).A group received chemotherapy through vein,the patients received docetaxel 75mg/m2 on day 1,tegafur 80mg/m2 on day 1 -14 and cisplatin 75mg/m2 on day 2 -4 of 21 -day courses.B group received hyperthermia together with chemothera-py.Hyperthermia was maintained at 40 -42℃ for 90min.The efficacy and toxicity -side -effect after the patients received two courses were evaluated.Results In A group,the rate of complete remission(CR)was 0 case,partial remission(PR)in 28 cases,stable disease(SD)in 27 cases,progressive disease(PD)in 8 cases,and the overall response rate(CR +PR)was 44.4%,while those in B group were 3 cases,36 cases,21 cases,3 cases and 61.9%, respectively.There was significant difference between the two groups(χ2 =4.589,P =0.032).The improvement rate of life quality of B group(58.7%)was better than A group(33.3%),there was statistically significant difference between the two groups(χ2 =9.232,P =0.002).The rates of Ⅲ°+Ⅳ°abnormal liver and kidney function,myelo-suppression and gastrointestinal tract in A group were 19.0%,23.8% and 25.4%,those in B group were 15.9%, 20.6% and 30.2%,there were no statistically significant differences between the two groups (P =0.815,P =0.830, P =0.426).Conclusion Hyperthermia combined with chemotherapy is efficient and safe for advanced gastric carci-noma,and it is worthy of furthermore study and observation in clinic.

Objective To investigate the effect of sirolimus (SRL) on the expression of integrin ανβ3 mRNA in vascular smooth muscle cells of cardiac allografts in rats, and the possible mechanism of SRL in the prevention of cardiac allograft vasculopathy. Methods Heterotopic heart transplantation models were established. Hearts from Wistar rats were heterotopically transplanted to SD rats (allograft) or Wistar rats (isograft). All rats were randomly divided into four groups:SRL group, SRL 1.25 mg/kg every day,oral intubation after cardiac transplantation; cyclosporine (CsA) group,CsA 10mg/kg every day, subcutaneous administration ; isograft group, no immunosuppressant administration;control group, normal hearts of Wistar rats. All of the animals were killed at 60 day after transplantation. Angiostenosis degree was analyzed by Mias system 4.1 after the cardiac tissues were stained with Van Gieson stain. Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect the expression of integrin ανβ3 mRNA in cardiac allografts. The relationship between the expression of integrin ανβ3 mRNA and the area of vascular stenosis was analyzed. ResultsIn control group, there was no thickened vascular intima and no vascular stenosis. In allograft group,the thickness of vascular intima was slightly increased but no vascular stenosis was detected. In CsA group, the vascular intima was obviously thickened, the lumen was narrowed, and some small arteries had obstruction. In SRL group, vascular intima proliferation and angiostenosis degree were obviously milder than in CsA group. Semiquantitative RT-PCR analysis revealed that the relative expression of integrin ανβ3 mRNA in CsA and SRL groups was increased significantly as compared with control group and isograft group (2.51±0.43 or 1.17±0.12 vs 0.28±0.08 or 0. 38 ± 0. 05, P＜0. 01). The relative expression of integrin ανβ3 mRNA in SRL group was decreased significantly as compared with CsA group (1.17±0.12 vs 2.51±0.43, P＜0.05). A positive correlation was found between vascular stenosis and the expression of integrin ανβ3 mRNA (r = 0. 654, P＜0. 01) in allografts. Conclusion SRL may down-regulate the expression of integrin ανβ3 mRNA in cardiac allografts in rats, which was probably associated with classic cardiac allograft vasculopathy.

Objective It is being increasingly recognized that electronic management of health information(eHealth) contributes greatly to safe medical treatment,high efficiency and better quality of health care.Definitive standards are required for medical information systems to share electronic health records.Methods The structure and contents of electronic health records were extensively investigated for sharing standards.The standards were evaluated in their functions and influences to the sharing of electronic health records.Results Suggestions were given to the standards of regional electronic health records.Conclusion Regional electronic health records facilitates individual medical care.

One important problem of regional information system in healthcare is the access to electronic patient records across healthcare institute boundaries. The Integrating Healthcare Enterprise initiatively proposes the integration profile of the retrieve information for display that enables a user to retrieve and display patients' related documents cross hospitals. The middleware is added to HIS to realize the cross-hospital data access to clinical information with access authorization and privacy regulations. Besides,the integration of cross-hospital patients' clinical information is achieved based on the realization of Patient ID protocol in different systems.

Aim To investigate the effect of 8-bromo-7-methoxychrysin(BrMChR) on apoptosis of human gastric carcinoma SGC-7901 cell line.Methods SGC-7901 cells were cultured and the inhibitory effect of BrMChR on the proliferation of SGC-7901 cell line was measured by MTT assay.The apoptosis of SGC-7901 cells induced by BrMChR was analyzed using flow cytometry (FCM) with PI staining.DNA ladder bands were observed by DNA agarose gel electrophoresis.Results MTT assay suggested that BrMChR markedly inhibited the proliferation of SGC-7901 cells in a concentration-dependent manner,its IC50 was 2.6 ?mol?L-1,the potency of BrMChR was 8 times more than that of lead compound,chrysin (ChR,IC50 was 16.5 ?mol?L-1),and was 3 times more than that of 5-fluorouracil (5-FU,IC50 was 7.7 ?mol?L-1). FCM with PI staining indicated the apoptotic rate of SGC-7901 cell line treated with BrMChR (1.25,5.00,20.00 ?mol?L-1) for 48 h was (19.8%?0.2%),(36.8%?1.9%) and (45.5%?3.5%) respectively and the rate treated with BrMChR (1.25 ?mol?L-1) was higher than that treated with ChR (20.00 ?mol?L-1,12.9%?1.5%). DNA agarose gel electrophoresis showed that when treated with BrMChR(20.00 ?mol?L-1) for 48 h,the gene DNA of SGC-7901 cells presented typical DNA ladder bands which could attenuated by the effect of pre-incubation of GW9662(10.00 ?mol?L-1),a blocker of PPAR?.Conclusion BrMChR might induce apoptosis of SGC-7901 cell line partially by activating PPAR?.

Objective To seek out the factors that influenced the mortality of population due to heat wave in Shanghai in summer(15 June-15 September). Methods Daily data of mortality of all causes, meteorological and air pollution in Shanghai in 1998 and 2003 were collected. Multivariate analysis was employed to investigate the relationship between mortality and heat wave intensity, duration, and timing within the summer season and air pollution concentrations. Results The heat wave in 1998 was more severe and caused a higher mortality than that in 2003. In 1998 heat wave, the heat wave duration,timing within the summer season were significantly associated with the daily number of deaths,while in 2003, besides the above two factors the daily maximum temperature also played an important role. Air pollution level slightly increased in heat wave and some factors such as air conditioner number, living space and urban greenbelt area could be used to explain the distinctive difference of heat related mortality between 1998 and 2003. Conclusion High temperature is the crucial factor for the high mortality of population due to heat wave in Shanghai in summer(15 June-15 September) in 1998 and 2003. Using air condition and enlarging living space will help to decrease the mortality.

Objective To discuss the application of role reversal teaching in the clinical course of rehabilitation medicine. Methods 64 students of a vocational college under clinical practice were divided into traditional teaching group (n=30) and role reversal teaching group (n=34). The achievement of knowledge and practice tests were compared after course. Results There was no significant difference in the score of knowledge test between the groups before clinical practice (P>0.05), however, the scores of both knowledge and practice tests were higher in the role reversal teaching group than in the traditional teaching group (P<0.001). Conclusion The role reversal teaching may improve the achievement of students for clinical course of rehabilitation medicine.

Objective:To study the drug distribution in the local tissues and lymph nodes treated with targeting and continuous interstitial chemotherapy for the oral squamous cell carcinoma before operation. Methods:8 patients with oral squamous cell carcinoma were treated by implanting the sustain-released cisplatin around and into the primary lesion. Drug distribution in tissue were studied by the graphite furnace atomic absorption spectrometry. Results:The drug concentration in the center of the tumor,1 cm and 2 cm from the tumor fringe were (34.877 1?15.720 8),(20.690 0 ?15.688 6) and (6.635 7?3.862 3) ?g/g, respectively. The drug concentration within lymph nodes of the sub-mental and submandibular area, deep cervical lymph nodes for the upper,middle and lower were(2.991 4?3.055 7),(4.026 0 ?3.692 0), (7.192 0 ? 8.958 7) and (5.028 5?3.484 3)?g/g, respectively. Conclusion:Targeting and continuous interstitial chemotherapy for the oral squamous cell carcinoma by implanting sustain-released cisplatin can increase drug concentration in the local tissues and the lymph nodes, to attain good efficacy of targeting chemotherapy.

Objective: To further investigate the molecular mechanism of photodynamic therapy. Methods: We used cDNA microarray technique to explore the gene expression profiles of HEPG2 cells after photodynamic therapy with hematoperphyrin monomethyl ether(HMME) in HEPG2 cells. After treated with HMME for 60 min, the HEPG2 cells were irradiated with laser, and observed by microscope with H E staining. To prepare the probes, mRNA from both control and treated cells were isolated and purified, then reversely transcribed to cDNA with the incorporation of fluorecent labeled dUTP. The probes were hybridized with a cDNA microarray representing the 1 538 genes originated from human hepatocarcinoma cells. The fluorencent signals of Cy3 and Cy5 were scanned and analyzed. Results: After laser irradiation, the HEPG2 cells showed the typical feature of apoptosis. The gene expression profiles were also changed greatly. Among the 1 538 target genes, 389(2.47%) different expression genes were detected. Most of the changed genes (nearly 80%) were down regulated. They were functionally related to cell proliferation cycle, replication, metabolism and so on. Several apoptosis associated genes were detected among those up regulated genes, encoding the key proteins involved in apoptosis signal transduction, such as CCP32,AIF,Mch2. Conclusion: The HMME photodynamic therapy can initiate the apoptosis process of HEPG2 cells, which may be regulated by mitochondial pathway.[