CD5+ B cells are derived from early stage precursors independently on bone marrow. It is an important subtype of B cells. Abnormalities of number and function of CD5+ B cells are found in several kinds of autoimmune diseases and B lymphocyte malignancies. Recently,the abnormalities are also found in AIDS,hepatitis, schizophrenia and the regeneration of liver cells, which indicates that CD5+ B cell may have extensively clinical significance.

The myocardial cell respiration and the activity of pyruvic acid dehydrogenase system and glutathione peroxidase (GSH-px) in blood weredetermined in rats fed by deficient selenium (Se) diet, supplement Se dietand Xian diet. The absorbed oxygen value of myocardial cell respirationwas 52. 5 ? 3. 78, 69. 75 ? 5. 34, 77. 63 ? 4. 38 O ?l/40min/100mg myocardium(mean ? SE ) in deficient Se group, supplement group, and xian group, re-spectively. The difference between deficient Se group and supplement Segroup or Xian group was significant (P

Objective To investigate the diagnostic and theraputic problems in patients with smooth muscle tumors of the duodenum.Method The clinical data from 7 cases smooth muscle tumors were analyzed retrospectively which were treated surgically and confirmed pathologically in our hospital from 1987 to 2001.Results Of all the cases,4 had a leiomyoma in the duodenum and 3 leiomysarcoma.This disease is lack of pathognomonic sign and difficult for early diagnosis.All the cases were treated surgically and got good results.Conclusions Upper gastrointestinal bleeding is common in patients with this disease.Upper gastrointestinal tract examination and gastrduodenoscopy and CT scan are the main methods for diagnosis.Operation is the best choice for treatment.

Objective To investigate the etiology, pathology, diagnosis and management of patients with fat necrosis of breast (FNB). Method The clinical data of 50 cases of FNB confirmed pathologically in our hospital from 1966 to 2001 were analyzed retrospectively. Results All the patients in this series were females with the average age of 32.5 years and 56% of the patients over 30 years. The main clinical feature was breast lump and some of the patients had a trauma history. 48 patients were treated surgically and other 2 got an accurate diagnosis by biopsy and were treated by non operative therapy. Conclusions FNB is easily misdiagnosed and difficult to be differentiated from breast cancer, thus the surgery or needle biopsy is recommended.

Dystonia is common in children with cerebral palsy. It can cause motor dysfunction, and is frequently associated with altered speech articulation, abnormal swallowing, and excessive drooling. Although trihexyphenidyl (Artane) is an anticholinergic agent with a long history of use in the management of dystonia in adults, information is limited regarding the use of trihexyphenidyl in children. This study reviewed the clinical experience of the use of trihexyphenidyl in children with cerebral palsy for dystonia, with special emphasis on benefits and tolerability.

Objective To summarize the ruptured types, operational ways and therapy effect for rupture of aortic sinus aneurysm.Methods The operational cured 55 cases with aneurysm of aortic sinus were retrospectively analysed.Results There were 46 cases of right aortic sinus aneurysm, 45 cases among them rupture was peretrated into right ventricle, one rupture was peretrated into right atrium;there were null aneurysm of aortic sinus, 5 cases among them ruptured into right atrium, 2 cases ruptured into right ventricle; 2 cases of left aneurysm of aortic sinus, ruptured into left ventricular. There were 24 cases with ventricular septal defect, 32 cases with aortic valvular imcompetence, 6 cases with other diseases, 8 cases complicated with infective endocarditis. All cases underwent operational treatment, one patient who had severe left heart failure and huge left ventricle before operation died from refractory arrhythemia after operation, another who had severe infective endocarditis,and patients with cardic function degree Ⅳ were died from low cardic output syndrome, others were better.Conclusions The right aneurysm of aortic sinus were the most types, and it always ruptured into right ventricle; It suggests that it is important for aneurysm of aortic sinus should be operated as early as possible in order to avoid losing the chance of operation, particular in the patients complicated with infective endocarditis .

Objective To observe the recent effect and influence of Lung Cancer Fang No.1 on immunologic function and serum vessel endothelia growth factor (VEGF), keratoprotein 21-1 (CYFRA21-1) in non-small cell lung cancer patients (NSCLC). Methods One hundred non-small cell lung cancer patients were randomized into 52 cases of treatment group on Lung Cancer Fang No.1 and 48 cases of chemotherapy group. The treatment group was treated by Lung Cancer Fang No.1, and the chemotherapy group by GP or TP programme for 2 treatment courses. Immune function indexes, serum VEGF and CYFRA21-1 were determined before and after treatment. Results Efficacy for the treatment group recently was 76.92%, better than 43.75% of the chemotherapy group (P

Aim To investigate the molecular mechanism of Rosiglitazone on the expression of integrin ?1 in lung pulmonary carcinoma A549 cell line. Methods RT-PCR and flow cytometry methods were used to examine the expression of mRNA and cell total protein of PPAR? and integrin ?1 subunit. Results The expression of PPAR? mRNA and integrin ?1 mRNA had dose-dependent regulating action by Rosiglitazone,using PPAR? inhibitor GW9662 and ERK pathway inhibitor PD98059 could abolish the inhibition of PPAR? on the expression of integrin ?1. Conclusion The effect of PPAR? ligand Rosiglitazone on integrin ?1 expression is mediated through PPAR?-dependent signaling parthway. The mechanism may be partly involved in ERK pathway.

BACKGROUND:Nitric oxide can interfere with the function of mitochondria, and accelerate the intervertebral disc damage and degeneration by interfering with the release of inflammatory cytokines. Nitric oxide is an important inflammatory cel medium leading to degeneration of intervertebral disc induced by pressure and other external factors.
OBJECTIVE:To investigate the regulatory effect of nitric oxide and nitric oxide synthase inhibitor niacinamide on mitochondrial function and its association with biological behavior of rabbit nucleus pulposus.
METHODS:Cultured nucleus pulposus cel s of rabbit lumbar intervertebral disc were randomly divided into six groups:normal blank control group, 10μmol/L sodium nitroprusside group, 100μmol/L sodium nitroprusside group, 200μmol/L sodium nitroprusside group, 0.05 g/L nicotinamide group (100μmol/L sodium nitroprusside+0.05g/L nicotinamide), and 0.5 g/L nicotinamide group (100μmol/L sodium nitroprusside and 0.5 g/L nicotinamide). Different doses of nitric oxide donor sodium nitroprusside and nicotinamide were added in the medium of each group. Three days after intervention, cel proliferation activity, intracel ular ATP concentration, cel nitric oxide synthase activity, cel ular reactive oxygen species level, and mitochondrial membrane potential were detected respectively.
RESULTS AND CONCLUSION:(1) After 3 days of rabbit nucleus pulposus cel s intervened by different concentrations of sodium nitroprusside, intracel ular nitric oxide synthase content increased with sodium nitroprusside volume increase, and ATP concentration decreased along with sodium nitroprusside volume increase;there were significantly differences between the normal control group and sodium nitroprusside groups (P<0.01). (2) Reactive oxygen species could be increased in the sodium nitroprusside group. Niacinamide groups indicated a dose-dependent manner to improve the increase of cel ular reactive oxygen species levels with sodium nitroprusside intervention (P<0.01). (3) In the sodium nitroprusside groups, nucleus pulposus cel membrane potential decreased. In the niacinamide groups, sodium nitroprusside-induced decline in mitochondrial membrane potential was reduced (P<0.01). (4) Niacinamide groups also indicated a dose-dependent manner to improve the proliferative activity of nucleus pulposus cel s as compared with sodium nitroprusside groups (P<0.01). Significant differences were determined between the two groups (P<0.01). (5) Results suggest that the excess nitric oxide can damage mitochondrial metabolic function of rabbit nucleus pulposus cel s and cause cel energy metabolism. Niacinamide can reverse these damages by inhibiting nitric oxide synthesis, thereby contributing to the prevention against intervertebral disc degeneration.

Objective To observe the operative effects of coronary artery fistula. Methods Malformations and operative results of 23 cases with coronary artery fistula were retrospectively analyzed. Results There were 13 cases of fistula of right coronary artery, 9 cases of fistula of left coronary artery, one cases of fistula of two coronary artery; 9 cases of coronary artery fistula opened into right ventricle,6 cases opened into right atrium, 3 cases opened into pulmonary artery, 2 cases opened into the sinus of coronary veins, 2 cases opened into left atrium, 2 cases opened into left ventricle, 3 cases were multiple fistulas. All cases received surgical treatment, excepttion for one cases of multiple fistulas had small residue fistula, the others were better. Conclusion At pressent,the operation treatment is the best method for fistula of coronary artery .