The efferent projections of the rostral ventrolateral medulla(RVL) to the hypothalamic paraventricular nucleus(Pa) and the thoracic cord were studied in the adult cat by using WGA-HRP or fluorescent retrograde tract-tracing method. After injection of WGA-HRP or fluorescent tracer Fast blue(FB) into one side of the Pa, retrogradely labelled cells were found in bilateral RVL, with an ipsilateral predominance. The labelled cells decreased in number from the caudal to the rostral level. After injection of FB into one side of the thoracic cord at T_2-T_3 segments, retrogradely labelled cells in the RVL were observed which increased in number from the caudal to the rostral level and reached the peak at 1.0-1.5mm caudal to the trapezoid body. Most of these cells were distributed in the ipsilateral RVL, and clustered in the region 0.0-1.0mm from the ventral surface of the medulla. After Diamino yellow 2HC1 and FB were injected into the Pa and the thoracic cord respectively, only single labelled cells were detected in the RVL, no double labelled cells were found. The above results suggest that the Pa and the thoracic cord receive separate fiber projections from different cells of the RVL.

In presnt experiment, animal models for hyperthyroidism and hypothyroidism were made, and using radioimmunoassay, the immunoeactive leucine-enkephalin (Ir-LEK) levels of such discrete brain areas as hypothalamus, striatum, cerebral cortex(frontal lobe) and whole pituitary were determined in different groups. The results obtained indicated that both hyperthyroidism and hypothyroidism induced significant changes of concentration in CNS Ir-LEK. It was found in hypothyroid group that concentration of Ir-LEK in hypothalamus, cerebral cortex and pituitary increased significantly against the control (P

Objective To evaluate the clinical effect and safety of the thrombus aspirated catheter (DIVER~(TM)) in primary PCI for acute myocardial infarction. Methods Seventy four AMI patients with total occluded infarct-related coronary artery(IRA)and intra-coronary thrombus from March 2005 to April 2006 were divided into two groups according to admission time and the treatment they received. After the 0.014 in BMW coronary wire crossed the lesion, the thrombus aspirated catheter (DIVERTM) was advanced over the wire to the lesion and continuously aspirated 2-3 times until the thrombus disappeared and stents were directly implanted in patients in group A (n=36) admitted from December 2005 to April 2006. Routine PCI applied in patients in group B (n=38) admitted from March 2005 to December 2005. Sirolimus eluting stents were implanted in both groups. The artery re-opened rate, no-reflow rate and the rate of major adverse cardiac events during hospitalization and 6 months after discharge were compared between two groups. Results Coronary arteriography showed the artery re-opened rate was 100% in both groups. The rates of no-reflow and major adverse cardiac events during hospital stay were significantly lower in group A than those in group B (2.78% vs 21.05% and 2.78% vs 10.53% respectively,P

The distribution of serotonin(5-HT), phenylethanolamine-N-methyltransferase (PNMT), substance P(SP) and leu-enkephalin(L-ENK) immunoreactive neurons in the rostral ventrolateral medulla (RVL) of the cat was studied with the immunohistochemical ABC technique, and the projection of 5-HT, SP and L-ENK positive neurons of the RVL into the thoracic cord was preliminarily investigated by a combined fluorescent retrograde transport and immunofluorescence method. The results indicate that 5-HT, PNMT, SP and L-ENK immunoreactive neurons mentioned above were localized primarily in the caudal part of nucleus paragigantocellularis lateralis and the rostral part of nucleus lateralis reticularis. SP positive cell bodies in the reticular formation close ventrolateral to nucleus ambiguus were also found. Some 5-HT, SP and L-ENK positive cells were situated in the area near the pia mater. In the most area of the RVL, 5-HT, PNMT, SP and L-ENK immunoreactive cell bodies had an overlapping distribution. 5-HT or PNMT or L-ENK positive neurons crowded, intertwined each other with their processes in the region at the levels 1.0-3.5mm caudal to trapezoid body, about 3.3mm lateral to the midline and about 0.8mm from the ventral surface of the medulla, and formed a longer or shorter continuous cell column which ran in the rostrocaudal direction. These three columns nearly coincided with each other at the level 1.5-2.5mm caudal to trapezoid body. Part of 5-HT, SP and L-ENK positive neurons in the RVL projected into the thoracic cord. The functional significance of these substances in the RVL was also discussed.

Objective To explore the effect of Baogan Jiedu Granule on liver cell ultrastructure of model mice with acute liver injury induced by tetracycline.Methods Totally 60 mice were randomly divided into tetracycline model group,large-dosage,medium-dosage and small-dosage groups of Baogan Jiedu Granule,Ganlixin group and control group.Each group was respectively perfused with corresponding drug for 5 days and tetracycline was perfused once to make the model.The tissues were observed by electron microscope,and the liver cell ultrastructure was measured and analyzed by three-dimensional metrology.Results The amount of mitochondria Vv,heterochromatin Vv,mitochondria ?m,endoplasmic reticulum ?m and Golgi's apparatus ?m all obviously decreased in model group,while that of euchromatin Vv and lipid droplet Vv increased.Baogan Jiedu Granule could remarkably increase the amount of indexes referred above,and decrease the amount of euchromatin Vv and lipid droplet Vv.Conclusion Baogan Jiedu Granule can significantly ameliorate the pathologic change of liver cell ultrastructure,thus having a good hepatoprotective effect.

To evaluate the inhibitory effect of recombinant adenoviruses p16 (Ad p16) combined with Ad p53 on human cholangiocarcinoma cell line QBC939 in vitro. Expression of p16 was low and p53 was negative in QBC939 cells. High level of p16 and p53 expressions were observed in QBC939 cell line transfected with Ad p16 and Ad p53. The growth rates of the Ad p16 infected combined with Ad p53 infected QBC939 cells were inhibited, which was higher than those of the Ad p53 or close to Ad p16 transfection. Colony formation in Ad p16 with Ad p53 transfected cells greatly decreased versus Ad p16 transfected or Ad p53 transfected cells. The Ad p16, Ad p53 and Ad p16 with Ad p53 transfected cells manifested apoptosis and G 1 arrest, which were confirmed by the flow cytometry. The suppression effects mediated by expression of the exogenous p16 with p53 in tumor cell resulted mainly from apoptosis and G 1 arrest, which is higher than exogenous p16 or p53.

OBJECTIVE: To improve the quality standards of Yishen wufa oral solution. METHODS:Polygonum multiflorum, Psoraleae corylifolia and Lycium barbarum in the formulation were identified by TLC qualitatively. The content of stilbene glycoside was determined by HPLC. RESULTS: TLC identification was specific. The TLC spots were clear and well-separated. The linear range of stilbene glycoside was 0.03～0.60 ?g(r=0.999 9)with an average recovery of 102.4%(RSD=1.2%,n=6).CONCLUSION: Established method can be used for the quality control of Yishen wufa oral solution.

Objective To investigate the characteristics of the pharmacodynamics of rocuronium bromide in patients with hepatobiliary disease. Methods Forty-eight patients undergoing abdominal surgery were divided into four groups, group A: patients with liver cirrhosis and portal hypertension (n = 12); group B1 : patients with cholelithiasis and obstructive jaundice (n = 12); group B2 : patients with cholelithiasis but no obstructive jaundice ( n=12);group C: patients without hepatobiliary disease ( n = 12) . Their renal function was normal. Patients with cardiovascular and neurological diseases were excluded. Premedication consisted of intramuscular phenobarbital 0.1 g and scopolamine 0.3 mg. Anesthesia was induced with midazolam 0.05 mg?kg-1 , fentanyl 2?g?kg-1 , propofol 2 mg?kg-1 and rocuronium 0.6 mg?kg-1 . The patients were intubated and mechanically ventilated. PETCO2 was maintained at 30-35 mm Hg. Anesthesia was maintained with iv infusion of propofol and fentanyl. Additional bolus dose of rocuronium 0.15 mg?kg-1 was given when T, recovered to 25% and each patient received six additional doses irrespective of duration of operation. Neuromuscular function was monitored using Datex-Ohmeda NMT mechanosensor. Onset time (from the end of injection to maximum depression of muscle twitch), clinical duration of intubating and additional dose (25 % recovery of T1 ) and recovery index (T1 from 25 % -75 %) were recorded. Results The demographic data were comparable among the four groups. The onset time was significantly longer in group A than that in group B, , B2 and C ( P

Objective:For further investigating the diagnostic value of the ECochG in Meniere's disease.Method:112 vertigo patients including Meniere's disease, sudden deafness, cervical vertigo and other vertigo dis-ease, had been done ECochG,glycerol test,audiometry and caloric test. The results were compared with those ofnormal group. Result:In Meniere's disease,the positive rate of abnormal－SP/AP in ECochG was 54.69% andthe positive rate of glycerol test was 53.12％. There are no statistics difference ( P ＞0.05) between this twotests with X analysis. Conclusion :We think that the glycerol test and other relative examination are necessary forthe diagnosis of Meniere's disease while the－SP/AP >0.4 ms in the examination of ECochG.

Objective To study the expression of RUNX3 mRNA in primary liver cancer (PHC) tissue and its surrounding normal tissue,and its clinical significance.MethodsReverse transcription polymerase chain reaction (RT-PCR) was performed to detect the expression of RUNX3 mRNA in tumor and peritumor tissues in 51 patients with PHC.The relationship between RUNX3 mRNA expression and some clinical pathological parameters was analyzed.ResultsThe relative expression values of RUNX3 mRNA in the tumor tissue and the surrounding normal tissue were 0.4509±0.0963 and 0.9147± 0.0222,respectively.The difference was significant (t=33.6087,P＜0.001).The RUNX3 mRNA expression in tumor tissue correlated with some clinical pathological parameters including low tumor differentiation,positive cancer embolus and intrahepatic invasion and metastasis.The RUNX3 mRNA expression was not correlated with other clinicopathological parameters including gender,cancer diameter,cancer location,hemorrhage and necrosis of cancer,and histotype.ConclusionRUNX3 may be a new tumor suppressor gene for PHC.