By analyzing different cooperation modes in the pharmaceutical R&D and innovation between Hong Kong and Guangdong province, traditional cooperation mode and R&D outsourcing mode cannot adapt to the glob-al competition in modern pharmaceutical R&D and innovation field. The perfect cooperation mode is mainly through the mergers and acquisitions of large-scale enterprise groups, and joint ventures with research institutions, scientific research agency, and other corporate; through establishment of large pharmaceutical enterprise group be-tween the two regions; and through establishment of front shop-back factory mode. To effectively achieve this goal, Guangdong government should remove compartmentalization and multi-sectors management system in the pharmaceutical R&D and innovation system, promote mergers and acquisitions between pharmaceutical companies through capital market, and set up a pricing mechanism of pharmaceutical R&D and innovation for different prod-ucts and different development stage.

Objective To investigate the relationship between high-density lipoprotein cholesterol (HDL-C) and idiopathic pulmonary embolism.Methods Clinical data of 34 patients with idiopathic pulmonary embolism from January 2005 to March 2011 were enrolled and healthy people with gender,age and basic diseasematched at the same period were selected as control.The venous blood samples of all the subjects were taken at 7:00 am after 10 hours of fasting and analyzed for total cholesterol (TC),lower-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C).Results Compared with healthy people,patients had reduced HDL-C level with a statistical significance ( ( 1.10 ± 0.28 ) mmol/L vs ( 1.49 ± 0.35 ) mmol/L,t =- 5.427,P ＜ 0.05 ).However,no statistically significant differences were found in the levels of TC and LDL- C (P ＞ 0.05 ).HDL-C was correlated with idiopathic pulmonary embolism( r =0.284,P ＜ 0.05 ).Conclusion The levels of HDL-C,but not TC or LDL-C,might be associated with the idiopathic pulmonary embolism.

Matrix metalloproteinase-8 (MMP-8) is a collagen which belongs to matrix metalloproteinase.Its function is that degrades collagen and be involved in inflammation.In breast cancer,the level of expression of MMP-8 is related to the invasion and metastasis of breast cancer as well as the clinical and pathological stage of breast cancer,but it is unclear on the molecular mechanism of MMP-8 in the malignant tumor.The research of expression in breast cancer prognosis of breast cancer will provide a reliable assessment of molecular marker.

ObjectiveTo evaluate the clinical effect of artificial femoral head replacement in treatment of unstable intertrochanteric femoral fractures.MethodsThe clinical data of 52 unstable intertrochanteric femoral fractures patients with artificial femoral head replacement were retrospectivly analyzed.Postoperative complications and postoperative weight-bearing time were recorded.The function of joints was evaluated with Harris score.ResultsAll patients were followed up for 6 to 24 months ,an average of 13.2 months.1 case was a lung infection after surgery,and 3 cases were deep vein thrombosis.Weight-bearing walking time averaged 28 days.According to Harris score:25 cases were rated as excellent, 18 good,7 fair,2 poor.A total of 82.7％ of patients had their joint function returned to normal or near normal.ConclusionIt was an effective mothod to treat the unstable intertrochanteric fractures in elderly patients with hemiarthroplasty.

Objective To discuss clinical effect and clinical application value of lianqi capsules treated with the middle-late stage primary liver cancer. Method We choosed 168 cases of middle-late stage primary liver cancer patients in Guang'an District Hospital,Sichuan province,Guang'an treatment, and they were randomly divided into observation group and control group. Both two groups were treated with the same chemotherapy regimens. Pingxiaopian were auxiliarily used in control group, and lianqi capsules in observation group. Clinical curative effect and security of two groups were observed. Result There was no statistically significant difference between the total effective rate of clinical treatment group and control group. There were statistically significant differences between two groups on the quality of life period (P<0.05) and adverse reactions (P<0.05). Conclusion Lianqi capsules combined with chemotherapy was used in treatment of patients with locally advanced primary liver cancer, and it can effectively improve the patients’quality of life in the whole process, control the growth of the tumor, and increased synergistic effect of chemotherapy for patients. So Lianqi capsules was worthy in clinical application and popularization.

It is well known that adolescent varicocele is one reason to induce the testicular hypotrophy and potential infertility.Its indication and methods of treatment are controversial.This review provides the recent progress in diagnosis and treatment of adolescent varicocele including the indications and methods of surgical treatment,as well as new information of future researcher in the areas.

Objective To evaluate the effects and security of single or combined sufentanil application on sedation and analgesia for mechanically ventilated patients in intensive care unit(ICU).Method A total of 160 ICU patients with mechanical ventilation were randomly allocated into four groups:sufentanil group,sufentanil plus midazolam group,sufentanil plus dexmedetomidine group and fentanyl group.Facial pain scale(FPS),Ramsay score and vital signs were employed to assess the effects of different therapies on organ functions and adverse reactions.Result After the treatment,FPS in all groups were significantly decreased and maintained around 3 points.Ramsay scores were significantly increased(P <0.001)and the score in group given sufentanil combined with midazolam or dexmedetomidine at the time points of30 min,1 h,6 h were even higher.Compared with the preoperative data,indicators of lung function(PaCO2and blood lactic acid),liver function(AST)and renal function(Cr)were decreased in all groups(P <0.05).Respiratory rate in the fentanyl group was significantly higher than the others.Total consumption ofsufentanil in group with combined sufentanil application was significantly lower.No significant differencesin adverse reactions were noticed among the groups(P >0.05).Conclusion For mechanically ventilatedpatients,sufentanil has efficient sedative and analgesic effects with less physiological interference and severe adverse reactions.Combined application is beneficial in decreasing total sufentanil consumption andinhibition to respiratory system,which is worthy of being spread.

LI-cadherins are a new member of cadherin superfamily,which transmembrane glycoproteins mediate Ca2 + dependent cell-cell adhesion independent of cytoplasmic interactions.They play an pivotal role during tissue development and are critical for the maintenance of junctional complexes between cells and tissues,LI-cadherin high expression in hepatocellular carcinoma results in the decrease of intercellular adhesion effect,cause hepatocellular carcinoma metastasis.

Cyclophilin A（CyPA） is a cytoplasmic protein which binds to immunosuppressive drug—cyclosporin A and possesses peptidyl-prolyl cis-trans isomerase activity. CyPA incorporated into virions through interactions with the Gag polyprotein in the life cycle of human immunodeficiency virus type 1(HIV-1) and its incorporation is essential for viral infectivity. This review discussed the function of CyPA in replication of HIV-1 virion and the usefulness of these studies for anti-HIV therapies.

Objective To establish a method for content determination of hyodeoxycholic acid in Qingkailing Injection. Methods An UltimateXB-C18 column (5 ?m, 250 mm?4.6 mm) was used with a mobile phase of acetonitril-water-phosphoric acid (35∶65∶0.1). The flow rate was 1 mL/min. The temperature of the column was 40 ℃. The detection wavelength was 192 nm. Results There was a good linear relationship between the concentration of hyodeoxycholic acid and absorption area value in range of 0.201 35～1.006 75 mg/mL, r=0.999 6. The average recovery was 98.25% with RSD=1.26%. Conclusion This method was accurate, credible and repeatable which can be used to control the quality of Qingkailing Injection.