ObjectiveTo study the effect of nutritional intervention combined with aerobic exercises on serum adiponectin and insulin resistance in nonalcoholic fatty liver (NAFL) patients.Methods32 patients confirmed as NAFL between April 2008 and August 2009 were enrolled into NAFL group and 31 health people into control group.NAFL group received nutritional intervention and followed aerobic exercise program.The changes of body mass index (BMI),serum adiponectin,fasting blood glucose (FBG),fasting insulin (FINS) and serum lipids were recorded before and after interventions.Insulin resistance index (HOMA-IR) was calculated.Inter-group difference,changes after intervention,and correlation between serum adiponectin and the other indexes were analyzed.ResultsCompared with the control group,BMI [ (29.84 ±3.16) kg/m2 vs.(22.22 ± 1.81 ) kg/m2,P =0.000],total cholesterol (TC) [ (5.09 ±0.86) mmol/L vs.(4.38 ±0.85) mmol/L,P =0.002],triglyceride (TG) [ (1.99±0.86) mmol/Lvs.(1.10±0.38) mmol/L,P=0.000],FBG[ (6.02±0.63) mmol/Lvs.(5.02±0.71) mmol/L,P=0.000],FINS[ (13.73±3.24) mU/Lvs.(9.55±1.99) mU/L,P=0.000],HOMA-IR [ (3.74±1.21) mmol/L vs.(2.08 ±0.27) mmol/L,P=0.000] in the NAFL group were significantly higher,while serum adiponectin in the NAFL group was significantly lower [ (4.89 ± 1.81 ) ug/L vs.( 12.97 ± 3.15 ) ug/L,P =0.000 ].After nutritional intervention and aerobic exercises,BMI [ ( 26.65 ± 2.39 ) kg,/m2 vs.(29.84 ±3.16) kg/m2,P =0.000] 、FBG [ (5.61 ±0.47) mmol/L vs.(6.02 ±0.63) mmol/L,P =0.004 ],and FINS [ ( 11.12 ± 2.01 ) mU/L vs.( 13.73 ± 3.24) mU/L,P =0.000 ] in the NAFL group were decreased significantly,while serum adiponectin [ (6.54 ±2.12) ug/L vs.(4.89 ± 1.81 ) ug/L,P =0.001 ] was significantly higher than the level before intervention.Serum adiponectin was negatively correlated with BMI,FBG,FINS,HOMA-IR,and TG (P =0.004,0.000,0.000,0.000,and 0.010),and positively correlated with high density lipoprotein cholesterol (P =0.026),but not correlated with TC (P =0.101 ).ConclusionThe combination of nutritional intervention and aerobic exercises can improve the level of serum adiponectin,adjust the level of serum glucose and serum lipids,and alleviate insulin resistance in NAFL patients.

Objective To study the relationship between serum adiponection and liver function and insulin level in patients with hepatogenous diabetes.Methods The research subjects included:28 patients with hepatog-enous diabetes who were admitted at Liaocheng People's Hospital (hepatogenous diabetes group);27 patients with simple type 2 diabetes without any complication (simple type 2 diabetes group),and 27 normal controb (control group).Blood adiponectin,fasting blood glucose (FBG),fasting insulins (FINS),alanine aminotransferase (ALT),aspartate transferase (AST),total bilirubin (TBIL) and albumin (ALB) were measured,and according-ly the insulin sensitivity index (ISI) was calculated.Results Compared with the control group.serum adiponec-tin levels and ISI levels were significantly lower in the other two groups (P<0.01),and the adiponectin levels were significantly higher in hepatogenous diabetes group than in simple type 2 diabetes group (P<0.05).The FBG and FINS levels in the hepatogenous diabetes group and the FBG levels in the simple type 2 diabetes group were significantly higher than those in control group (P<0.01).The FINS levels were significantly higher in the hepatogenous diabetes group than in simple diabetes group (P<0.05),while the levels of FBG and ISI were lower than that of simple type 2 diabetes group (P<0.05).Adiponectin levels were negatively correlated with ISI (r= 0.5265,P=0.000),and positively correlated with FBG (r=-0.6040,P=0.000),and were not correlated with liver functions Conclusion Insulin resistance and abnormal metabolism of adipenection exist in patients with hepatogenous diabetes and may together affect the occurrence and development of hepatogenous diabetes.

Objective:To observe the effect of nutritional intervention in patients with hepatic diabetes.Methods:57 patients were enrolled and randomly divided into treatment group(n=29) and control group(n=28).On the base of same liver medicine,the control group received insulin therapy while the treatment group received strict nutrition intervention.Results:The level of fasting blood glucose and blood glucose 2 hours after meal and glycosylated hemoglobin were decreased significantly in treatment group(P

OBJECTIVE: To develop a rapid, accurate and sensitive method for the determination of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally. METHODS: The LC-MS method was used to detect the extractive of Chinese patent medicine for antihypertension in respects of relative molecular mass, tandem mass spectrometry fragment, retention time, UV spectrum. The compounds added into the Chinese patent medicine were identified by comparing with standard sample in terms of spectrum, chromatographic and mass spectrometric behavior. RESULTS: According to four aspects of determination, nifedipine, nitrendipine and nimodipine were found in three kinds of Chinese patent medicine for antihypertension. CONCLUSION: The method is selective and sensitive for the detection of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally.

Objective To study the expression of RUNX3 mRNA in primary liver cancer (PHC) tissue and its surrounding normal tissue,and its clinical significance.MethodsReverse transcription polymerase chain reaction (RT-PCR) was performed to detect the expression of RUNX3 mRNA in tumor and peritumor tissues in 51 patients with PHC.The relationship between RUNX3 mRNA expression and some clinical pathological parameters was analyzed.ResultsThe relative expression values of RUNX3 mRNA in the tumor tissue and the surrounding normal tissue were 0.4509±0.0963 and 0.9147± 0.0222,respectively.The difference was significant (t=33.6087,P＜0.001).The RUNX3 mRNA expression in tumor tissue correlated with some clinical pathological parameters including low tumor differentiation,positive cancer embolus and intrahepatic invasion and metastasis.The RUNX3 mRNA expression was not correlated with other clinicopathological parameters including gender,cancer diameter,cancer location,hemorrhage and necrosis of cancer,and histotype.ConclusionRUNX3 may be a new tumor suppressor gene for PHC.

Objective To evaluate the relationship between retinal nerve fiber layer (RNFL)thickness measured by optical coherence tomography (OCT) and the refractive error and age using partial correlation analysis. Methods A total of 106 right eyes of healthy subjects, comprising 33 eyes with high myopia ( spherical equivalent [SE] < - 6. 0 D) , 60 eyes with moderate myopia ( - 6. 0 D ≤ SE < - 3.0 D) and 13 eyes with low myopia and emmetropia ( -3.0 D≤SE <0. 5 D) were analyzed in this cross-sectional study. Mean clock hour, quadrant and total average RNFL thicknesses were measured by OCT and compared among the three groups respectively. Associations among RNFL measurements and spherical equivalent and age were evaluated by partial correlation analysis. Results The mean RNFL thickness measurement in high myopia(95.74 ± 13.46) μm was significantly lower than that in moderate myopia group ( 101.43 ± 11.53 ) μm, low myopia group and emmetropia group ( 108. 06 ± 8.42) μm ( P < 0. 05 ). Positive correlations were found between RNFL measurements at 1, 5, 6 and 12 o'clock, superior and inferior quadrant, and total average RNFL and SE ( r = 0. 36,0. 33,0. 43,0. 29,0. 28,0. 39, P < 0. 01, r = 0. 22,P <0. 05), while negative correlations were found between RNFL measurements at 8, 9 and 10 o'clock and temporal quadrant and SE ( r = -0.21, P <0.05, r = -0.36, P <0.01; r = -0.24, P <0.05; r = -0. 30, P <0. 01 ). RNFL measurements at 2, 3, 4 and 6 o'clock and nasal and inferior quadrant varied with age ( r = -0.20,-0.20,-0.20,P <0.05,r =0.31,P <0.01). Conclusions RNFL measurements varied with the refractive error and age. Analysis of RNFL thickness in the evaluation of glaucoma should always be interpreted with reference to the refractive status and age. In the same time, the location of the RNFL should be noted.

Objective:To explore the establishment of the comprehensive and designing experiment and to evaluate its effect in practice teaching.Method:During the intensive training before practice,the comprehensive and designing experiment was applied to the 339 students from 2002 clinical medical speciality.Results:More than 70% students thought that the new teaching mode could stimulate their interests in study;majority of the teachers agreed with the new teaching mode.The hospitals where the subjects worked after their graduation feed backed that the subjects from 2002 clinical medical speciality had more advantages than the other students.Conclusion:Application of comprehension experiment and experiment-designing course can increase the effect of students' learning and cultivate their comprehended qualities.

Objective The aim of the study was to examine the influence of clonidine and ketorolac pretreatment on cytokine response to abdominal surgery. Methods Twenty patients scheduled for upper-abdominal surgery were randomly allocated to two equal groups: (1) pre-emptive analgesia group: patients received oral clonidine 4?g/kg and ketorolac 10mg 1h before surgery and (2) control group: patients received placebo. Blood samples were taken 24h before surgery , 20min after induction of anesthesia (before surgery), 90min after induction (during surgery), and 0,1,2,4,6 and 24h after surgery for determination of serum tumor necrosis factor-? (TNF-?), interleukin-1?(IL-1?),interleukin-6(IL-6) and interleukin-10 (IL-10) concentrations.Results (1) In the control group, serum IL-6 concentration increased significantly during surgery and peaked at 2h after skin closure. In contrast serum IL-6 concentration in pre-emptive analgesia group increased significantly when the skin was closed and peaked at 4h after skin closure. IL-6 production in the pre-emptive analgesia group was less than that in control group at 0,1 and 2h after surgery(P

OBJECTIVE:To establish a turbidimetric method for the determination of the potency of the crude drugs and tablets of oxytetracycline. METHODS: By turbidimetric method in which Staphylococcus aureus was used as the test organism with biomass concentration of 1.0%～1.5% (V/V). The culture temperature was 37 ℃ and the culture time was about 4 h. The method was compared with the cup-plate method in potency. RESULTS: The linear range of oxytetracycline was 0.05～0.4 IU?mL-1. The average recovery of the crude drug of oxytetracycline was 99.59% (RSD=2.0%) and that of oxytetracycline tablets was 99.51% (RSD=1.91%), showing no significant difference as compared with the cup-plate method in contents. CONCLUSIONS: The method was sensitive and rapid yet with few influencing factors thus applicable for the determination of the potencies of oxytetracycline and its tablets.

OBJECTIVE:To determine the titer of main component in kitasamycin tablets and acetylkitasamycin dry suspension.METHODS:Staphylococcus aureus was used as test organism and which were cultured at (37?0.5) ℃ for about 4～5 hours by adding 1.0%～1.5% kitasamycin and 2.0%～2.5% acetylkitasamycin,then the absorbance was determined at a wavelength of 530 nm.RESULTS:The linear range of kitasamycin was 0.5～4.0 IU?mL-1 and that of acetylkitasamycin was 0.45～6.3 IU?mL-1,and their recovery rates were 101.7%(RSD=1.9%,n=9) and 101.1%(RSD=2.1%,n=9),respectively.CONCLUSION:The established turbidimetry is sensitive,rapid yet with few influencing factors,thus it is applicable for the titer determination of main component in kitasamycin tablets and acetylkitasamycin dry suspension.