OBJECTIVE:To explore the way out for the continuing development of hospital preparations.METHODS:We analyzed the current situation of hospital preparations and the existing problems in pharmaceutical research related to hospital preparations and discussed the feasible developmental direction of hospital preparations.RESULTS:To achieve a continuing development for hospital preparations,hospitals have to improve their current technology for preparations,closely combine the characteristics of hospitals to prepare preparations targeted to the needs of the clinic,shift to the development of new drugs on the basis of the existing preparations and carry out pharmaceutical work and basic study closely related to clinical pharmacy.CONCLUSION:Transformation from a simple supply type to a technology-based supply type and exploitation research type will be a feasible trend for the development of hospital preparations.

Methylenetetrahydrofolate reductase(MTHFR),a key enzyme in methionine-folic acid metabolism,participates in the synthesis of thymidine monophosphate(TMP) and purine as an indirect donator of methylium.It plays an important role in maintaining the normal level of homocysteine and DNA repair and replication.The MTHFR gene mutation decreases the activity of enzymes,increases the homocysteine level of blood plasma,and induces abnormal DNA methylation and various diseases.This review focuses on the structure and function of the MTHFR gene,its polymorphism and the relationship between its polymorphism and congenital heart disease.

OBJECTIVE: To summarize the cause and mechanic of inflammation reaction induced by medical biomaterials in the body.DATA SOURCE: A computer-based online search of Pubmed database was undertaken to identify the relevant articles published from January 2004 to December 2008 in English with the key words of "biomaterials, inflammation, mechanism".Meanwhile, with Chinese articles were retrieved from CNKI database between January 2005 and December 2008 with the key words of "biomaterials, inflammation, mechanism".DATA SELECTION: Articles concerning the cause and mechanic of inflammation reaction induced by medical biomaterials were included. Articles addressing physical and chemical features and mechanics of medical biomaterials were excluded.MAIN OUTCOME MEASREUS:①After the material implants in vivo, inflammatory cell and material adhering.②Cell factor expression.RESULTS: After initially examined 70 literatures were obtained. According to inclusion and exclusion criteria, the reasons and the mechanism related to the medical biological material in host in vivo for causing the inflammation were analyzed. Along with the tissue engineering development, various new high-polymer medicine biological materials with good biological compatibility,the biological activity and the biodegradation absorption function are emerging unceasingly. The biomedicine material was used to repair or substitute damage organization and the organ, and to cause function recovery. The infection was still the serious complication following the medical biological material was implanted. The mechanism of biomedicine material-induced infection is: complement in blood adhere the biological material surface, which causes collection and adherence of integrin-mediated leukocytes, release of cytokines and growth factors from adherent monocytes/macrophages, resulting in the occurrence of biomedicine material-related infection. To prevent the medical biological material-related inflammation, we should first embark from the material itself, and seek for some to respond the small medical material to the host inflammation.CONCLUSION: The success of material implantation depends on the medical biological material relevant inflammation. The production of biomaterial-related inflammation is action results of inflammatory cell, inflammation factor, complement as well as enzyme, oxygen free radical. Material surface microstructure, chemistry and dielectric and so on immediately influence inflammatory cells on material response.

This paper introduces the design of automatic biochemistry analyzer based on CAN field bus. The hardware and software of a single network node and several problems are narrated. Some related flow charts and source codes are also given. The involvement of CAN bus in the design of automatic biochemistry analyzer enhances the efficiency and the degree of automatization.

OBJECTIVE:To apply the kinetic turbidimetric Limulus test to the endotoxin assay of Levocarnitine sodium chloride injection.METHODS:The16-fold dilution of Levocarnitine sodium chloride injection was prepared.The content of bacterial endotoxin in Levocarnitine sodium chloride injection was determined with kinetic turbidimetric Limulus test after screen test and validation test.RESULTS:The16-fold dilution of Levocarnitine sodium chloride injection was effective to e?liminate the interference in Limulus test.The average recovery was in the range of50%～200%.CONCLUSION:The kinetic turbidimetric Limulus test provides a new and quick method for the quantitative determination of bacterial endotoxin in Levo?carnitine sodium chloride injection.

Objective To evaluate incisor root resorption of adults with skeletal classⅢmalocclusion through three-dimensional reconstruction of cone beam computed tomography (CBCT) image using interacting medical imaging control sys?tem (Mimics) and then offer guidance for labially tilted angle control in orthodontic treatment and distance of bone moving in surgery. Methods Adults of skeletal class Ⅲ malocclusion (n=30) who underwent orthodontic-orthognathic combination treatment were included in this study. CBCT images were then reconstructed three-dimensionally using Mimics 10.01. Each incisor were separated at cementum-enamel junction as crown and root. Labially tilted angle and root volumes of each inci?sor were measured before and after operations. At last, the correlativity between root resorption and labially tilted angle was calculated through statistic analysis. Results The decrement of upper, lower and front incisors’root volumes upon treat?ment is statistically significant. However, there is no statistically significant difference of incisor ’s root volumes among eight incisors nor between different gender. There is linear correlation between root volumes and labially tilted angle when the lat?ter changes 7° or more when they followed the fomular =-21.416+5.618X. Conclusion For adults with skeletal classⅢmalocclusion, orthodontic treatment before orthognathic surgery decrease incisor root volumes when labially tilted angle changes 7° or more.

Stomatology is an applied science,and experimental teaching plays an important role in training renovation talents.A complete construction of the laboratory system is a complex systematic project,which requires the right teaching conception,advanced experimental instruments,high-quality teachers of laboratory,the optimization of the teaching contents,as well as highly efficient operating mechanism and management approach.It is also a process of exploration and innovation.

Objective To study the effects of intraperitoneal adherence of pus mosses on the efficacy of open repair(OR)and laparoscopic repair(LR)for perforated peptic ulcers(PPUs). Methods Intraperitonealad-herence of pus mosses was divided into the first,second and third stage,respectively. Surgical duration,volume of peritoneal drainage ,rate of abnormal wound healing ,number of intensive care unit transfer ,recovery time of bowel function ,ambulation time ,length of hospital stay ,and postoperative treatment abandonment rate were compared. Results There were 78 ,46 ,and 29 patients for the three stages in OR group ,and 71 ,39 ,and 15 patients in LR group,respectively. There were significant differences insurgical duration,volume of peritoneal drainage,rate of abnormalwound healing,number of ICU transfer,recovery time of bowel function,ambulation time,length of hospital stay about the first stage between the two surgical methods(P values were 0.000,0.000, 0.015,0.000,0.000,0.000,and 0.043 respectively)except for treatment abandonment rate(P value was 0.209). There were significant differences in volume of peritoneal drainage ,rate of abnormal wound healing ,number of ICU transfer,recovery time of bowel function,ambulation time,and length of hospital stay(P values were 0.000, 0.033,0.000,0.000,0.001,and 0.028 respectively)for the second stage except surgical duration and treatment abandonment rate(P values were 0.196 and 0.358 respectively) between the two procedures. The differences were significant in surgical duration ,volume of peritoneal drainage ,and length of hospital stay between the two methods (P values were 0.039 ,0.003 ,0.024 ,and 0.002 respectively) for the third stage but there were no significant differencesin rate of abnormal wound healing , number of ICU transfer , recovery time of bowel function ,ambulation time ,and treatment abandonment rate (P values were 0.175 ,0.173 ,0.766 ,and 0.757 respectively). Conclusions LR is superior to OR under the first stage of pus moss and still has advantages under the second stage. However,LR may be inferior to OR under the third stage.

Objective:To investigate research topics and management methods for hospital pharmacy in order to promote the devel -opment of hospital pharmacy researches .Methods:The basic principles , main research fields , strategies and approaches of topic se-lection in hospital pharmacy , as well as the main points of research management were discussed .Results:The main research fields of hospital pharmacy included experimental studies and soft science researches .The principles of selected topics should follow practicabil-ity, scientificity, innovation, feasibility and effectiveness , and the topics should set off from the thinking path of pharmacy , be for the purpose of translational medicine and be based on the practical problems of clinical drug use .The quality evaluation of generic drugs , druggability research and so on could take advantage of close to clinics of hospital pharmacy , which may be the focus aspects .It is nec-essary to establish a continuous improvement of performance inspection system , improve the construction of echelon personnel and drive mechanism, and strengthen the scientific research cooperation inside and outside the team in hospital pharmacy researches .Conclu-sion:The scientific researches on hospital pharmacy are very important to develop pharmacy , which are worthy of further exploration .

OBJECTIVE:To extract and purify organic acids with macroporous resins from Folium Isatidis.METHODS:Folium Isatidis was extracted with 70% aqueous ethanol(pH=2)by Soxhlet extraction.The process was optimized by employing orthogonal test design with the content of anthranilic acid as the index.RESULTS:The resin HPD100 had much better adsorption capacity and desorption ratio by using 3 BV 60% aqueous ethanol to extract organic acids in Folium Isatidis than other resins.The results showed that HPD100 possessed the better ecomic under the concentration of sample 0.18mg?mL-1,the condition of pH 3.5,and flowing rate of 2BV?h-1.CONCLUSION:Macroporous resin HPD100 may be value of isolating and purifying the organic acids from Folium Isatidis.