Objective To investigate the expression of p27kip1, CyclinE, CyclinD1 in bladder transitional cell carcinoma (BTCC), and evaluate their relationship with turnout cell proliferation. Methods To study the expression of p27kip1, CyclinE and CyclinD1 protein in 41 cases of paraffin-embedded BTCC tissue and in 12 cases of cystitis tissues by immunohistochemical method SP. Results There was a negative relationship between p27kip1 expression and clinical stage and pathological grade.There was no relationship with lymph node metastasis (P>0.05). There was a positive relationship between CyclinE expression and clinical stage, pathological grade and lymph node metastasis (P<0.05). There was no relationship between CyclinD1 expression and clinical stage, pathological grade and lymph node metastasis (P>0.05). Conclusion The results of this study showed that the abnormal expression of p27kip1, CyclinE, may play important roles in the genesis and progression of BTCC. CyclinD1 may take activation in the early term of BTCC.

Objective To analyze the clinical characteristics of pulmonary infection in patients with multiple myeloma for improving early prevention,diagnosis and treatment.Methods A retrospective analysis was conducted for 70 patients with multiple myeloma admitted to our hospital from January 2012 to April 2015.The clinical data of pulmonary infection were reviewed and analyzed in terms of radiological findings,pathogen distribution,and related risk factors.Results The peripheral white blood cell count and neutrophil percentage could be normal in pulmonary infection of patients with multiple myeloma.However,erythrocyte sedimentation rate increased significantly.Radiological study revealed that infection of bilateral lungs was common.The most frequently identified pathogens were gram negative bacteria,especially Pseudomonas aeruginosa.The main predisposing factors of pulmonary infection were agranulocytosis,stage Ⅲ multiple myeloma,and complications.Conclusions The clinical symptoms of pulmonary infection are diverse in patients with multiple myeloma.Poor immunity is the primary predisposing factor.The common pathogens are gram-negative bacteria.Beta-lactam/beta-lactamase inhibitor combinations or fluoroquinolones are effective empiric treatment for controlling the progression of pulmonary infection.

Objective To investigate the detection rate of malnutrition among post-stroke patients in community hospitals and unravel the relevant factors that precipitate malnutrition after stroke. Methods Based on 438 post-stroke patients who were admitted in community hospitals, we examined the demographic characteristics, the nutritional indices and the possible malnutrition related factors through a cross-sectional study.Results The detection rate of malnutrition among post-stroke patients was 52.7%. Group comparison through multivariate logistic regression analysis showed that there was a higher malnutrition detection rate in the post-stroke patients with multiple stroke attacks (three stroke attackes and above, OR = 11.00,95%CI 1. 14-106.34), higher NIHSS scores (group with NIHSS≥15, OR=7.09, 95% CI 2.90-17.36) , higher modified Rankin scales (group mRS 4-5, OR = 15. 77,95% CI 6.61-37.59) (trend test P<0.0001) .The risk of malnutrition was also correlated with the post-stroke depression, poorer family care, no early-stage rehabilitation, history of malignant tumors and severe alcoholism. Conclusions There is a high detection rate of malnutrition among post-stroke patients in community hospitals. There are many factors related to malnutrition among post-stroke patients in the community. More attention to controllable influencing factors would improve the prognosis of post-stroke patients.

Objective: To determine the national norm of Chinese questionnaire of quality of life in Chinese patients with cardiovascular diseases (CQQC) according patients with hypertension, coronary artery disease（CAD）, and congestive heart failure（CHF）and to analyze the influence factors. Methods: The quality of life was estimated by CQQC to evaluate 7937 persons normal and with cardiovascular diseases（contain with hypertension, CAD, and CHF ） in 28 hospitals of 19 cities across China. The data were compared in gender, age, exercise, psychologic condition, education, number of family member and medical cost. Results: 1、The mean scores（national norms）of normal persons were （95.74±23.21）; 2、The scores of hypertension patients were （73.75±23.62）, the scores of hypertension stage 3 group （69.06±21.78）were least compared with other stages（P<0.01）; 3、The scores of CAD patients were（64.67±20.59）, the scores of unstable angina group（55.37±18.62）were least compared with other types in CAD patients（P<0.01）; 4、The scores of CHF patients were （40.36±17.24）, it was least compared with those of hypertension and CAD patients （P<0.01）; 5、Influence factor: （1）Sex, age: The mean scores were （71.41±27.8）for 7937 persons, the scores of male persons were significantly higher than those of female (P=0.000). In normal persons, along with age increased, the scores decreased （P<0.05） ; except ≥70 ages group, the scores of male persons were higher than those of female（P<0.05） ; （2）Exercise: The scores of patients participating exercise were significantly higher than those of no participating exercise [（72.64±21.87）vs.（66.91±23.13）, P=0.000]; （3）Psychological condition: The scores of patients with healthy psychological condition were significantly higher than those of with psychologic disorder [（70.13±25.66）vs.（68.91±20.44）, P=0.001]; （4）Other: patients with better education, more than 1 family members and medical insurance or public expense had more scores (P<0.05～0.001). Conclusions： The sample size is large and the datum is reliable for Chinese questionnaire of quality of life in Chinese patients with cardiovascular diseases. It can reflect influence of age, sex, exercise, psychologic condition, family, education background, medical condition and disease, its item is brief, clear, and operation is easy, so it may well be spread.

OBJECTIVETo study the characteristics, location, and amino acid changes of novel mutations of the Dystrophin gene.

METHODSTwelve patients in whom no deletion or duplication of the Dystrophin gene was detected were analyzed with next-generation sequencing. Fifty healthy adult males were recruited as the controls.

RESULTSAll patients were detected with mutations of the Dystrophin gene, which included c.33C>G, c.583C>T, c.1333C>T, c.2593C>T, c.5731A>T, c.7288G>T, c.2803+1G>T, c.10034G>A, c.4289A>G, c.1905_906delAG, c.5017delC, c.5768_5771delAAGA, and c.6261_6262insA. No similar mutations were found among the controls.

CONCLUSIONOur data has enriched the mutation spectrum of the Dystrophin gene and may provide an important basis for genetic diagnosis.

Child ; Child, Preschool ; Dystrophin ; genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Male ; Mutation

Objective To investigate the correlations of high mobility group protein box-1 (HMGB1) level with severity and prognoses of acute cerebral infarction.MethodsBetween April 2018 and October 2018, 300 patients with acute cerebral infarction and 122 healthy control subjects were enrolled. According to National Institute of Health stroke scale (NIHSS) scores, patients with acute cerebral infarction were divided into group A (NIHSS scores<5), group B (5≤NIHSS scores≤15) and group C (NIHSS scores≥16). According to modified Rankin Scale (mRS) scores, patients were divided into good prognosis group (mRS scores≤2) and poor prognosis group (mRS scores>2) after 3 months of follow up. The serum levels of HMGB1, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in different groups were detected and compared. Pearson correlation analysis and receiver operating characteristic curve were used to analyze the correlations of serum HMGB1 level with other indicators and evaluate their predictive values in poor prognosis.Results The serum HMGB1 level in the acute cerebral infarction patients was significantly higher than that in the controls ([7.98±3.99]μg/Lvs. [4.61±1.02]μg/L,P<0.05); the serum HMGB1 level in the group C was significantly higher than that in group B ([12.86±1.91]μg/Lvs. [7.30±1.07]μg/L,P<0.05), and that in group B was statistically higher than that in group A ([7.30±1.07]μg/Lvs. [3.78±0.95]μg/L,P<0.05). Serum HMGB1 level was positively correlated with LDL-C level and NIHSS scores (r=0.521,P=0.000;r=0.931,P=0.000), and negatively correlated with HDL-C level (r=-0.114,P=0.001). The serum HMGB1 level in good prognosis group was significantly lower than that in poor prognosis group ([6.52± 3.29]μg/Lvs. [9.88±4.03]μg/L,P<0.05), and the serum HMGB1 level was positively correlated with mRS scores (r=0.160,P=0.000). The area under the curve of HMGB1 predicting poor prognosis of acute cerebral infarction (0.736[95％CI: 0.677-0.795]) was larger than that under the curve of LDL-C predicting poor prognosis of acute cerebral infarction (0.634[95％CI: 0.570-0.698]).ConclusionSerum HMGB1 level in patients with acute cerebral infarction is significantly increased, which is related to severity of disease, and has certain predictive value in prognoses of acute cerebral infarction.

To investigate the experience and efficacy of endoscopic thyroidectomy for papillary thyroid microcarcinoma (PTMC) through total areola approach.
 Methods: A total of 117 PTMC patients, who were diagnosed pathologically in Minimally Invasive Surgical Center, Second Xiangya Hospital, Central South University from June 2016 to December 2017, were divided into a endoscopic surgery group (n=72) and an open surgery group (n=45). The number of dissected central lymph nodes, blood loss, amount of drainage, occurrence of postoperative complication and recurrence were collected and compared.
 Results: Compared with the open surgery group, the blood loss was less and the operative time was longer in the endoscopic surgery group (P<0.05). There were no significant differences between the 2 groups in the number of dissected central lymph nodes, amount of drainage and occurrence of postoperative complication (all P>0.05). The mean follow-up time was more than 20 months, and there was no recurrence in the 2 groups. 
 Conclusion: Endoscopic thyroidectomy with central compartment neck dissection through total areola approach is safe and feasible in patients with PTMC. It has many advantages, such as no scar on neck, less blood loss, shorter hospital stay and more acceptable to young patients.
Carcinoma, Papillary ; surgery ; Endoscopy ; Humans ; Nipples ; Thyroid Neoplasms ; surgery ; Thyroidectomy