Objective To explore and analyze effect of heparin combined with urokinase therapy on lower limb venous thrombosis.Methods 90 cases with lower extremity venous thrombosis were chosed and divided into control group of 45 cases and experimental group of 45 cases according with random number table method.Control group was given pure low molecular heparin treatment, experimental group was treated with small dose of urokinase static drop on the basis of control group, 7 days for a course, a total of 2 course of treatment.Compared two groups before and after treatment of lower limb venous patency rate changes, blood coagulation function index, adverse reactions and the recurrence rate.Results Compared with control group post-treatment.patency rate of posterior divisions, popliteal vein and posterior tibial vein of experimental group were significantly better (P<0.05).Experimental group post-treatment compared with control group post-treatment, PT, APTT, TT time had significant difference ( P <0.05 ).Patients incidence of adverse reactions were significantly lower than control group ( P <0.05 ).Patients were followed up for 1 year recurrence rate was significantly lower than control group ( P <0.05 ).Conclusion Effect of low molecular heparin combined with urokinase in the treatment of venous thrombosis of lower limbs significant and its prognosis is well.

Objective:To compare the contents of notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 between Notoginseng Radix et Rhizoma and its formula granules. Methods:An HPLC method was used with a SunFire C18 column (250mm × 4. 6 mm, 5μm),the flow rate was 1. 0 ml·min-1, the detection wavelength was set at 203 nm, and the column temperature was at 30 ℃. The mobile phase was acetonitrile( A)-water( B) with gradient elution. An HPLC was used to determine the contents of the three ingredi-ents between Notoginseng Radix et Rhizoma and its formula granules, and compare the differences. Results: The total content of the three ingredients in Notoginseng Radix et Rhizoma and its formula granules was 9. 214% and 8. 646%, respectively. The total content of the three ingredients was equivalent and the daily amount of the major components in the commercial formula granules was equivalent with that in the decoction of Notoginseng Radix et Rhizoma. Conclusion:The production process of the original formula granules is re-liable, and the quality of formula granules of Notoginseng Radix et Rhizoma is stable.

Objective:To establish an HPLC method for the determination of baicalin and polydatin in Kanggan Liyan syrups. Methods:The samples were analyzed on an Waters SunFire C18 column with the mobile phase A of acetonitrile and the mobile phase B of 0. 2% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 284nm,and the column box temperature was 30℃. Results:Baicalin and polydatin could be separated effectively without interference. The linear range of baicalin was 32. 0-480. 0 μg·ml-1 and the average recovery was 98. 71%(RSD=0. 67%,n=5). The linear range of poly-datin was 16. 0-240. 0 μg·ml-1 and the average recovery was 97. 02%(RSD=1. 03%,n=5). Conclusion:The method is accurate and stable, and can be used in the determination of Kanggan Liyan syrups.

Objective To explore the sanitation of centralized drinking water supply in rural area of Guangxi. Methods 400 centralized drinking water supply systems in 33 counties (district) in rural area of Guangxi had been randomly investigated. The field survey on each monitoring site was carried out by investigation form. The water sampling from distribution system and determination were carried out based on the related national standards. Results The rural centralized water supply facility covered 1 170 147 people in this survey. The national and non-national debt project on rural water supply system accounted for 100 and 300 respectively. The rural centralized water supply system with fully and partially treatment (coagulation, deposition, filtration or disinfection) accounted for 48.00%, and those with the fully treatment accounted for 16.00%. The rural centralized water supply systems of national debt projects with fully and partially treatment (coagulation, deposition, filtration or disinfection) accounted for 33.00%, which is statistically higher than those (10.33%) of non-national debt project. The ratio of the rural centralized water supply systems with fully and partially treatment between national and non-national debt project revealed statistically significant difference (P

Objective To know the environmental lead pollution status of rural areas around the mining area in Guangxi,2008.Methods From August to December,2008,the survey was carried out in 28 villages of 4 counties,which were located in basin of three rivers and their tributaries.Six lead-zinc-tin mines were situated in the investigated area.The local samples were collected,including 101 rice samples,30 paddy samples,59 vegetable samples and 45 samples of meat,viscera of poultry,5 fish samples,and 27 surface water samples,12 ground water samples,13 drinking water samples,29 cropland soil samples.The lead content of them were determined,the dietary survey on lead intake was performed among the residents living near the mining area also.Results 53.68%(102/190) of the crop samples revealed higher lead contents,which were over the standard limit,especially for rice(47.52%) and leafy vegetables(100%).The lead contents for five eggs samples,two peanuts samples and thirteen fresh corns were qualified.36%(102/190)of the samples of meat and viscera of poultry revealed higher lead contents,which were over the standard limit,especially for liver and chicken intestine.The lead intake only through rice and vegetables reached 4.55 mg in one week,which was over the tolerance dose by 0.52 times.Conclusion The lead intake of the residents living near the lead-zinc mining area are mainly from crop and vegetables and poultry.Environmental lead pollution is serious in the investigated area and must be controlled as possible as early.

Objective:To prepare Cuochuang Xiaoyan lotion and establish HPLC fingerprint chromatogram for the quality control . Methods:The separation was performed on a Waters XTerra MS C 18column(250 mm ×4.6 mm, 5μm).The mobile phase consisted of acetonitrile-0.2%phosphoric acid with gradient elution at a flow rate of 1.0 ml· min-1 , the eluent was monitored by a UV detector at 277 nm, and the column temperature was at 30℃.Results: There were sixteen common peaks for the sample , and among them, three ones were identified as baicalin , linarin and rhein , respectively .Conclusion:The repeatability and information of chromatogram peaks of the method are satisfied , which can provide credible quality control method for Cuochuang Xiaoyan lotion .

Objective:To establish an HPLC method for the fingerprint determination of glycyrrhizae dispensing granules. Meth-ods:Twelve samples were analyzed by HPLC with glycyrrhizic acid as the reference. The analysis was performed on a Waters SunFire C18 column(250 mm × 4. 6 mm,5 μm) with the mobile phase A of acetonitrile and the mobile phase B of 0. 1% phosphoric acid solu-tion with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 237nm, and the column temperature was 30℃. Results:By analyzing the fingerprint, 21 peaks existed including the peak of liquiritin and glycyrrhizic acid. The similarity of the samples was more than 0. 97. Conclusion:The established HPLC fingerprint of glycyrrhizae dispensing granules is dependable and simple. The method can provide scientific basis for the quality control of glycyrrhizae dispensing granules.

Objective:To establish the HPLC fingerprint for Polygonum cuspidatum dispensing granules. Methods:The HPLC fin-gerprint of 12 batches of Polygonum cuspidatum from different manufacturers were determined. The analysis was performed on a Waters SunFire C18 column(250 mm × 4. 6 mm,5μm)with acetonitrile as the mobile phase A and 0. 05% phosphoric acid solution as the mo-bile phase B with gradient elution. The flow rate was 1. 0 ml·min-1 ,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10μl. Results:The results were calculated according to“similarity evaluation system for tradi-tional Chinese medicine chromatographic fingerprint”nominated by CFDA combined with the analysis of the HPLC fingerprints. Totally 14 common peaks with similarity above 0. 98 were found in the HPLC fingerprint of Polygonum cuspidatum,including the peak respec-tively for polydatin and emodin. Conclusion:The method can provide more information for the quality control of Polygonum cuspidatum dispensing granules.

Objective:To establish the HPLC fingerprints of chemical constituents in Forsytiae suspensa Fructus from Shiyan dis-trict to provide scientific evidence for the quality control of Forsytiae suspensa Fructus. Methods: HPLC was performed on a Waters SunFire C18 (250 mm × 4. 6 mm, 5 μm) column and a Diamonsil C18 guard column, and the mobile phase consisted of acetonitrile-0. 4% acetic acid solution with gradient elution. The flow rate was 1 ml·min-1 , the detection wavelength was 277 nm, and the col-umn temperature was 30℃. Results:There were 14 common peaks in the HPLC fingerprints of chemical constituents in 12 batches of Forsytiae suspensa Fructus from Shiyan. The 14 characteristic peaks of Forsytiae suspensa Fructus from different habitats were under clustering analysis, and the similarity showed little difference. The contents of forsythoside A and forsythin exhibited significant differ-ence in the samples. Conclusion:The HPLC fingerprint method is simple, rapid and feasible in the quality control of Forsytiae suspen-sa Fructus.

Objective To explore the role and mechanism of keratin 19 (KRT19) in breast cancer.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to determine KRT19 levles in 35 cases of breast cancer tissues and normal tissues.The correlation between KRT19 levels and clinical property of breast cancer was analyzed.Meanwhile,the expression levels of KRT19 in several breast cancer cells and mammary epithelial cell Michigan cancer foundation (MCF)-10A were evaluated by qRT-PCR assay.Over-expressed and knocked-down KRT19 in breast cancer ceils,3-(4,5-dimenthylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to detect the proliferation and chemosensitivity of these cells.The ability of forming colon of these breast cancer cells with treated KRT19 was studied via colony-forming unit assays.Western blot assay was performed to determine expression levels of ceil cycler related proteins.Results KRT19 was upregulated in breast cancer tissues comparing to normal tissues.KRT19 was positively related to tumor node metastasis (TNM) stage and distant metastasis of breast cancer.Similarly,KRT19 was highly expressed in breast cancer cells compared to mammary epithelial cell MCF-10A.The proliferation and colony-forming ability was significantly enhanced in MCF-7 cell with o,verexpressed KRT19.MTS assay showed that chemosensitivity of MCF-7 cells with overexpression of KRT19 was much more remarkably reduced than the control group.However,knocking down KRT19 in breast cancer cells MDA-MB-231 got the opposite results.Western blot assay suggested that KRT19 could obviously upregulated cyclin-dependent kinase 1 (CDK1) but not p27 expression levels.Conclusions KRT19 was upregulated in breast cancer tissues and was positively related to TNM stage and distant metastasis of breast cancer.KRT19 can significantly enhance proliferation and chemoresistance of breast cancer cells via upregulating CDK1.