Objective: To evaluate the levels of 1ipoprotein lipase protein (LPL)and mRNA in cerebrospinal fluid (CSF) for children retinoblastoma(RB)and evaluation of the chemotherapy. Methods: Case-control study. Total 36 cases were collected in Beijing Tongren Hospital From October 2015 to May 2017. There were two groups, 19 cases of central nervous system(CNS) metastasis and 17 cases of non CNS metastasis according to laterality, age and gender. The changes of neuronspecific enolase (NSE) in serum and cerebrospinal fluid (CSF), chloride, glucose and quantitative protein and white blood cell count in CSF were compared between the two groups before initiating chemotherapy and after the third and sixth cycles of chemotherapy. LPL expression was assessed by Western blot and RT-PCR.Comparisonsbetweenthetwo groups of general data were performed usingt-test. The measurement data were expressed by mean ± standard deviation, and variance analysis was conducted. Results: The level of CSF-NSE from CNS metastasis group was significantly higher than non CNS metastasis group(F=16.43, P=0.002). The level of serum NSE from CNS metastasis group was significantly higher than non CNS metastasis group before chemotherapy(F=41.06, P=0.006). There were significant differences in the level of serum NSE in CNS metastasis group before and after chemotherapy (F=7.06, P=0.001). CSF-LPL protein expression in the CNS metastasis group was significantly higher than that of non CNS metastasis group (F=2.57, P=0.001). There were significant differences of LPL expression in CNS metastasis group before and after chemotherapy (F=2.63, P=0.003)). Conclusion The expression of LPL protein in CNS may be related to the progression and chemotherapyof RB with CNS metastasis. (Chin J Lab Med, 2018, 41: 608-614)

Objective To explore the correlation analysis of mobile nurse workstation with the acceptance of minority nursing staffs. Methods We designed the acceptance of mobile nurse workstation for minority nursing staffs scale to investigate 317 minority nursing staffs in one first class grade three hospital in Xinjiang. Results The total score of mobile nurse workstation among minority nursing staffs was ( 74. 93 ± 10. 55), which was above the average;the recognition, usage intention and smooth degree about nurse workstation had statistical significance with the acceptance of mobile nurse workstation (P<0. 05). Conclusions We should strengthen and promote the recognition, usage intention and smooth degree of mobile nurse workstation, so as to make mobile nurse workstation to be a tool suited whole nursing staffs and improve infromatization quality of nursing.