After purifying thyroglobulin (TG) and thyromicrosomal (TM) antigens,the ELISA (enzyme-linked immunosorbent assay) for detecting anti-TG,anti-TM,serum TG level,TG-antithyroglobulin immune complex (TG-IC) and IgG-,IgM-,IgA-specfic antithyroglobulin,antithyromicrosome were established.These specific immunplogical assays were sensitive,reproducible and did not have the shortcomings associated with the impurity of the TM antigen.The results of clinical studies in patients with Hashimoto's thyroiditis (n=35),hyperthyroidism (n=86),primary hypothyroidism (n=7) suggested that these specific assays were helpful to improve the diagnosis and investigation into pathogenesis for aformetioned autoimmune thyroid diseases (AITD).