Objective To observe the clinic efficacy of Shenling Baizhu Pill on patients with irritable bowel syndrome(IBS).Methods Ninety patients with IBS were collected and divided into Chinese patent medicine group and western medicine group by random number method,45 patients in each group,the Chinese patent medicine group received Shenling Baizhu pill group treatment,the western medicine group was gave Smectite Powder treatment.The changes of clinical symptoms,intestinal tract microorganism and serum inflammatory factor of patients were observed and compared between two groups before and after treatment.The clinical symptoms was evaluated by questionnaire, the Lactobacillus,Bifidobacterium,enterobacteria and yeast were determined by cultivation,the levels of IL -10,IL -18 and TNF -αwere detected by enzyme linked immunosorbent assay(ELISA).Results Two groups before treat-ment of anaerobic bacteria in feces reduction,aerobic bacteria increased serum IL -18 and TNF -alpha content, increased,the content of IL -10 decreased.Shenling Baizhu Pill intervention for 8 weeks in patients with intestinal anaerobic bacteria[(B/E 1.07 ±0.49)]and serum IL -10[(43.65 ±9.52)mg/L]content increased,aerobic bacte-ria[(B/E 1.07 ±0.49)],IL -18[(82.06 ±21.07)mg/L]and TNF -alpha[(28.44 ±10.20)mg/L]decreased. The IL -18 decreased the most,was different with the western medicine group,there was a statistical significance(t =9.926,P <0.01).Two groups had no bad reaction.Conclusion Shenling Baizhu Pill can effectively regulates and improves the intestinal micro -environment,promote the growth of intestinal probiotics and remodeling the intestinal function of biological barrier,decrease the levels of proinflammatory factors and rise the anti -inflammatory factors level,which has a good clinic effect in treating IBS.

Objective To explore the application value of INTERGROWTH-21st standard evaluate intrauterine growth restriction (IUGR).We aimed at predicting IUGR with ultrasonic fetal growth measurements.Methods A prospective cohort of singleton pregnancy scanned after 28 gestational weeks was constructed.The Z-score values of growth measurements were calculated using the INTERGROWTH-21st standard.Logistic regression equation was used to establish the prediction formula.Finally,the new formula was compared with the traditional Hadlock method to predict the accuracy of IUGR.We assessed outcomes for consenting participants who attended research scans and delivered at the Obstetrics and Gynecology Hospital of Fudan University.Results A total of 834 patients fulfilling the inclusion criteria were included.After the exclusion of 143 lost cases,565 of control cases and 126 of IUGR cases were obtained.The sensitivity,specificity,positive predictivevalue,negative predictive value,false positive rate and false negative rate of prediction model wererespectively:88.9 ％,85.4 ％,57.7 ％,97.2 ％,14.6 ％,11.1 ％.In contrast,the values of Hadlock wererespectively:81.7％,82.7％,51.2％,95.3％,17.3％,18.3％.Conclusions This study shows aprediction model of IUGR.INTERGROWTH-21st standard improved the diagnostic accuracy of IUGR,especially enhanced the positive predictive value.

Objective To observe the effect of hyperbaric oxygen (HBO) therapy on lumbar intertransverse fusion in rabbits. Methods Twenty-eight healthy adult female rabbits underwent lum-bar intertransverse fusion at L5～6 with autogenous iliac bone graft. They were randomly assigned into an HBO group and a control group (n=14). Rabbits were killed 6 weeks after the operation for surgical inspection, X-ray, bone density measurement and histologic analysis. Results The fusion rate of the HBO group evaluated by surgical inspection, and radiologic examination in a blind fashion significantly increased compared with that of the control group (P<0.05). Qualitative histologic as-sessment showed increased bone formation and bone maturity in the HBO group compared with the control group. Conclusion Hyperbaric oxygen therapy could improve the fusion rate of lumbar inter-transverse fusion in rabbits.

OBJECTIVE To investigate the effect of cadmium (Cd) exposure in vivo and in vitro on duodenal epithelial cells in mice and the mechanism. METHODS In vivo, C57BL/6 mice were ig administered with CdCI210 mg-kg" once per day for 30 d to establish a chronic cadmium poisoning model, or were ig administered with a single dose of CdCh 80 mg • kg-1 to establish an acute cadmium poisoning model before the survival status and survival rate of mice were observed. Duodenal epithelial cells of acute and chronic cadmium poisoning mice were isolated and cultured. The cells were identified as epithelial cells by E-cadherin immunofluorescence. Then, the content of cadmium ion in duodenal epithelial cells was detected by confocal laser microscopy. In vitro, duodenal epithelial cells of normal C57BL/6 mice were isolated and cultured, and incubated with CdCI2 2.5-100 pmol-L-1 for 24 h. CellTiter-Blue was used to detect cell viability. Subsequently, the duodenal epithelial cells of normal mice were incubated with CdCI215 Mmol • L-1 for 24 h, and cell cycle was detected by flow cytometry. Then, the duodenal epithelial cells of normal C57BL76 mice were incubated with CdCI230 Mmol-L'1 for 3-12 h and the expression of cleaved-caspase 3 was detected by Western blotting. RESULTS Compared with the control group, the activities of mice with chronic cadmium poisoning were all normal. The mice with acute cadmium poisoning showed depression, less food intake and death. At the 5th day of acute cadmium exposure, the survival rate of mice decreased to 40%. The content of cadmium ion in duodenal epithelial cells of acute and chronic cadmium poisoning mice increased significantly (F<0.01). Furthermore, CdCI2 inhibited the viability of duodenal epithelial cells cultured in vitro and the half inhibitory concentration (IC50) was (24.55±0.84) pmol-L-1. Compared with the control group, CdCI2 blocked the cell cycle of duodenal epithelial cells at Go/G, phase (P<0.05), and the expression of cleaved-caspase 3 in duodenal epithelial cells was significantly increased after CdCI2 treatment for 6, 9 and 12 h (P<0.05, P<0.01). CONCLUSION Cadmium that enters the body through the digestive tract can be absorbed by duodenal epithelial cells and cause damage to the cells. The mechanism of cadmium-induced damage may be related to cell cycle arrest and caspase 3-mediated apoptosis.

Objective To evaluate the effects of soy isoflavones on bone density (BMD) in women in randomized clinical trials by meta-analysis. Method We searched the databases the Medline, Pubmed, and CNKI from January 1990 to October 2007 using the keywords, phytoestrogen, isoflavone, soy, genistein in combination with bone. We only included the studies of randomized clinical trial, in which the data of BMDs at lumbar spine, total hip or femoral neck prior to and post isoflavone intervention or their relevant changes and their standard deviation or 95% CI in women were provided. Results Sixteen papers (1304 women, 91% postmenopausal) were included and a mean daily dose of 73 mg supplemental soy isoflavones resulted in weighted mean (%)(95%CI) difference in yearly BMD changes of 18.3 (2.0%,CI 6.0~30.6) and 3.3(0.40%,CI 0.5~6.1) mg/cm2 at the lumber spine and total hip, respectively. Subgroup analyses showed that the effects were more pronounced in those with the isoflavone dose ≥80 mg/d than those of

China ; Female ; Hepatitis A Vaccines ; administration & dosage ; Hepatitis B ; prevention & control ; Hepatitis B Vaccines ; administration & dosage ; Humans ; Male ; Vaccination ; methods ; Vaccines, Combined ; administration & dosage

As a typical food safety industrial model strain, Bacillus subtilis has been widely used in the field of metabolic engineering due to its non-pathogenicity, strong ability of extracellular protein secretion and no obvious codon preference. In recent years, with the rapid development of molecular biology and genetic engineering technology, a variety of research strategies and tools have been used to construct B. subtilis chassis cells for efficient synthesis of biological products. This review introduces the research progress of B. subtilis from the aspects of promoter engineering, gene editing, genetic circuit, cofactor engineering and pathway enzyme assembly. Then, we also summarized the application of B. subtilis in the production of biological products. Finally, the future research directions of B. subtilis are prospected.
Bacillus subtilis/genetics* ; Bacterial Proteins/genetics* ; Gene Editing ; Metabolic Engineering ; Promoter Regions, Genetic

Tumor-associated macrophages (TAMs) are one of the most abundant immune cells in the tumor microenvironment. As a heterogenous cell population, TAMs usually affect tumor growth, metastasis, and therapeutic resistance. Currently, TAM has attracted wide attention as a promising therapy target for human cancers. As one of the basic treatments of malignant tumors, radiotherapy can regulate the phenotypes and functions of TAM. Meanwhile, TAM can affect the response of cancer cells to radiation. This paper reviews the research advances of TAM in cancer radiotherapy.

L-homophenylalanine (L-HPA) is an important non-natural amino acid that has been used as a key intermediate for the synthesis of Puli drugs for the treatment of hypertension. At present, L-HPA is synthesized using chemical methods, which has the disadvantages of expensive raw materials, tedious steps and serious pollution. Therefore, researchers have conducted in-depth research on the enzymatic production of L-HPA. This review summarizes the research progress on the enzymatic synthesis of L-HPA, including the dehydrogenase process, the transaminase process, the hydantoinase process, and the decarboxylase process, with the hope to facilitate the industrial production of L-HPA.
Amino Acids ; Environmental Pollution ; Industry ; Protein Biosynthesis

To improve the productivity of L-phenyllactic acid (L-PLA), L-LcLDH1(Q88A/I229A), a Lactobacillus casei L-lactate dehydrogenase mutant, was successfully expressed in Pichia pastoris GS115. An NADH regeneration system in vitro was then constructed by coupling the recombinant (re) LcLDH1(Q88A/I229A) with a glucose 1-dehydrogenase for the asymmetric reduction of phenylpyruvate (PPA) to L-PLA. SDS-PAGE analysis showed that the apparent molecular weight of reLcLDH1(Q88A/I229A) was 36.8 kDa. And its specific activity was 270.5 U/mg, 42.9-fold higher than that of LcLDH1 (6.3 U/mg). The asymmetric reduction of PPA (100 mmol/L) was performed at 40 °C and pH 5.0 in an optimal biocatalytic system, containing 10 U/mL reLcLDH1(Q88A/I229A), 1 U/mL SyGDH, 2 mmol/L NAD⁺ and 120 mmol/L D-glucose, producing L-PLA with 99.8% yield and over 99% enantiomeric excess (ee). In addition, the space-time yield (STY) and average turnover frequency (aTOF) were as high as 9.5 g/(L·h) and 257.0 g/(g·h), respectively. The high productivity of reLcLDH1(Q88A/I229A) in the asymmetric reduction of PPA makes it a promising biocatalyst in the preparation of L-PLA.
L-Lactate Dehydrogenase ; genetics ; Lactobacillus casei ; enzymology ; genetics ; Phenylpyruvic Acids ; metabolism ; Pichia ; genetics ; Recombinant Proteins ; genetics ; metabolism