Objective To investigate the method and efficacy of treatment of displaced proximal humeral fracture. Methods The clinical data of 43 patients with displaced proximal humeral fracture underwent diagnosis and treatment in the latest 5 years were analysed retrospectively. Among them, twelve cases received conservative treatment and 31 cases were treated by operation. Results All patients had clinical union of humeral fracture during the follow up period of 6 months to 3 years. The necrosis of humeral head happened in 3 cases. The excellent and good rates of shoulder joint function were 80 6% in operation group and 75% in conservative group according to Gongyi Huang's assessment standard. Conclusion The treatment method should be chosen according to types of the humeral fracture and general condition of the patients. The main purpose of the treatment is to regain shoulder joint function.

OBJECTIVE: To prepare heparan sulfate-vitamin E succinate (HDV) amphipathic copolymers and explore the pharmaceutical properties of doxorubicin (DOX)-loaded HDV copolymer micelles (DOX/HDV). METHODS: HDV copolymers were prepared by amide reaction and its structure was confirmed by H-NMR. DOX/HDV micelles were prepared by ultrasonic method. The particle size, morphology, Zeta potential, drug loading, entrapment efficiency, and in vitro drug release and cytotoxicity were evaluated. RESULTS: HDV amphipathic copolymers were synthesized successfully. The particle size, PDI value and Zeta potential of drug-loaded micelles were (105.0±7.3) nm, (0.239±0.484) and (-21.4±2.6) mV, respectively. The encapsulation and drug loading rate were (76.22±0.76)% and (9.53±0.58)%, respectively. The results of drug release test in vitro showed that DOX was released slowly from the micelles. Cytotoxicity experiments indicated that blank micelles had no apparent toxicity against both tumor cells and normal cells. However, DOX/HDV micelles could inhibit the tumor cells growth obviously. CONCLUSION: HDV copolymers can effectively load DOX with properties of drug sustained release and enhanced cytotoxicity against tumor cells in vitro, which indicates that HDV may be a potential candidate for cancer therapy.

Aim To explore the effeet of puerarin (Pue) on aortic function and blood pressure in hyper-tensive mice induced by high-fat diet.Methods Thirty male mice were divided into five groups named as normal diet group ( Con ) , high-fat diet group (1)10), high-fat diet + low-dose puerarin group (20 mg 'kg-1 •(!"'), high-fat diet + medium-dose puera¬rin (40 mg • kg"1 • d ~1 ) group and high-fat diet + high-dose puerarin group (80 mg 'kg-1 • d~l).Hie mice were injected intraperitoneally with Pue for eight weeks.Body weight, blood pressure and blood glucose were measured.Serum was collected to detect blood lipid.Aortas were separated from aortic endothelial cells to test the vasodilative function.Aortic endotheli¬al cells from 1)10 mice were isolated to perform Iran-swell and cell proliferation experiments.Results I High-rlose puerarin treatment could reduce the body weight, body fat, blood glucose and blood pressure in obese mice ( P < 0.01 ) ; 2 High dose of puerarin could improve the vasodilative function of aortas com¬pared with those from 1)10 mice (P <0.01 ) ; (3) The migration ability of primary endothelial cells from 1)10 + Pue group was improved compared with that from 1)10 group (P <0.01 ).Conclusions Puerarin can significantly reduce blood pressure in obese mice in¬duced by high fat diet by improving the aortic diastolic function and endothelial cell proliferation and migra-tion.

Aim To investigate the intracellular disposition process of doxorubicin (DOX) in human breast cancer MCF-7, providing reference for explaining the pharmacology and their side effects of anti-tumor drugs. Methods The drug-resistant cell line MCF-7/DOX of breast cancer with DOX indication was selected as the material, and ultra-high performance liquid chromatography quadrupole tandem time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-MS/MS) method was established to analyze the disposal of DOX by target cells. Results Two unreported trace a-mounts of new metabolites of doxorubicin were found, and their structures were deduced by high-resolution multistage mass spectrometry. Molecular docking showed that its affinity for DNA was lower than that of DOX. Conclusion Target cells have unique and diverse drug metabolism pathways for DOX, which may be related to drug resistance mechanisms.

The construction of biosafety laboratory was highly concerned these years because of the spread of high pathogenic diseases including SARS and avian influenza. In the past several years, the construction of many BSL-2/3 laboratories played an important role in preventing the dissemination of pathogens involved in the research. Meanwhile, serious problems were also raised because some project designers or constructors were not clear with the compulsory safety equipments in these laboratories so that the requirement of zero-leakage could not be achieved. The compulsory safety equipments emerging and disposing solid, liquid and gas offal in the biosafety laboratory were reviewed. The R&D of special equipments in biosafety laboratory in China was also introduced.

The spices, with a wide range of remarkable pharmacological effects, were limited in developing clinical drugs because of their poor solubility, high irritation and low bioavailability. Controlled release preparations can delay the release of drugs on the basis of solubility enhancement to improve bioavailability. The research on spice ingredient-loaded controlled release preparations was sum-marized in the paper for the further development of spice active ingredients.

Vaccinium bracteatum, an evergreen shrub of Vaccinium genus of Ericaceae, is widely distributed throughout China, especially in Jiangsu, Zhejiang, and Fujian provinces. At present, the researches on V. bracteatum mainly focus on the extraction, separation and bioactive verification of the functional components in its leaves and fruits. However, few literatures had been reported on its application in foods. This paper summarized the main functional components of its leaves and fruits. The development and utilization of the leaves and fruits were also introduced, which is helpful for the development and application of V. bracteatum resources.

Gardeniae Fructus is the mature fruit of Gardenia jasminoides from genus Gardenia Ellis, nom. cons., Rubiaceae Family. The fruit of G. jasminoides is one of the widely used Chinese herbals and also can be used as edible resource. Geniposide and crocin are the two mainly components in the fruit, and some bioactivities of them had been proved. The protective effects and their mechanism of geniposide and crocin on the central nervous system were summarized in this paper, including diseases (Alzheimer's disease, Parkinson's disease and cerebral ischemia) and physiologic function (learning and memory), which was helpful to develop and utilize the fruit.

Heme peroxidase EfeB in E. coli belongs to the dye-decolorizing peroxidase (DyP) superfamily. Peroxidases in this superfamily have a good ability in degradation of synthetic dyes, but their physiological functions in organisms are unclear. In order to further understand the physiological function of EfeB, the mutant strain EcoΔefeB was constructed by homologous recombination. The differences between parental strain E. coli BL21 and EcoΔefeB at genome transcription level as well as cell growth under different conditions were compared. The response of efeB to iron ion was also investigated. The results showed that the deletion of efeB gene caused the differential expression of 1 765 genes, which were mainly related to cell metabolic pathway, cell membrane synthesis and flagellum movement. There was no significant difference in cell growth between BL21 and EcoΔefeB at pH 7. 0, but the growth of BL21 was much better than that of EcoΔefeB at pH 4. 5. The functional expression of efeB may support the survival of E. coli at low pH. EfeB was significantly up-regulated when Fe

Objective: To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R. Br. ex Sabine fruits. Methods: The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains. Total phenolic, flavonoid, monomeric anthocyanin and tannin contents, and antioxidant activities were determined by spectrometric methods. Results: The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content (4. 329.66 mg of gallic acid/100 g), while seed extract showed the high antioxidant activity and total tannin content (5. 326.78 mg catechin equivalent/100 g). Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila, Bacillus subtilis, Escherichia coli O157:H7, Pseudomonas aeruginosa, Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL. However, seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract. Conclusions: Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed. These results support that these plant extracts can be used for the treatment of bacterial infections.