Objective To explore the effect of different hemostatic methods on ovarian function in la-paroscopic ovarian endometriosis cyst enucleation. Methods 200 cases of stage I and II ovarian endometriosis cyst admitted to our hospital from Jan. 2012 to Apr. 2014 were selected. All patients underwent conventional la-paroscopic ovarian endometrial endometriosis cyst enucleation. According to intraoperative hemostasis methods, patients were divided into 3 groups: suture group (n=70), electric coagulation group (n=70), and ultrasonic scalpel group (n=60). Follicle-stimulating hormone (FSH) and antibody forming cells (AFC) on admission and at 1, 3, 6, and 12 months after surgery were recorded. Peak systolic blood flow of ovarian stromal artery (PSV) was also observed. Results FSH for all the three groups were improved compared with that on admission, and the difference had statistical significance (P<0.05). FSH for the suture group was significantly lower than that in the electric coagulation group and ultrasound knife group at 1, 3, 6, and 12 months after operation. The difference had statistical significance (P<0.05). AFC at one month after surgery had no statistical significance between the 3 groups (P>0.05). however, AFC of the suture group at 3, 6, and 12 months after surgery was significantly im-proved compared with those of the the electric coagulation group and ultrasound knife group. The difference had statistical significance (P<0.05). Vaginal type B ultrasonic examination after operation showed that PSV of the su-ture group was higher than that of the electric coagulation group and ultrasound knife group at the second menstru-ation and at the 6-month menstruation after surgery. The difference of PSV between the 3 groups had statistical significance (P<0.05). Conclusion Compared electric coagulation hemostasis and ultrasonic scalpel hemostasis, the suture method can reduce damage of ovarian function in patients with ovarian endometriosis cyst, which is worth to promote.