Objective To establish a HPLC method for the separation and determination of stachydrine hydrochloride in fresh medicinal material of Herba Leonuri.Methods The NH2 column(250 mm? 4.6 mm,5 ? m) was adopted with acetonitrile-water(80 ∶ 20) as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was at 192 nm.The column temperature was at 22 ℃.The solution for content determination was obtained after extracted with ethanol and then filtered and purified through an activated charcoal-aluminium oxide column with ethanol as eluant.Results Stachydrine hydrochloride in fresh Herba Leonuri can be separated and determined on NH2 column.A good linear range was within 2.29～ 11.46 ? g.The average recovery for stachydrine hydrochloride was 98.26 % and RSD was 1.52 %(n=5).Conclusion The method is steady and with good repeatability,and can be used to determine the content of stachydrine hydrochloride in fresh Herba Leonuri.

OBJECTIVETo establish integration processing method for pretreating and vinegar producing Corydalis yanhusuo.

METHODDifferent processing methods were contrasted with the traditional processing technology, and contents of corydalis B, water extract and ethanol extract in samples of different processing products were determined.

RESULTThe content of corydalis B were best in the samples of vacuumizing C. yanhusuo chips scaked in rice vinegar for twice or soaked in rice vinegar after chip drying. The water extract was highest in the samples of chip soaked in rice vinegar after drying, followed with chip vacuumizing twice, and there were no remarkable difference between the other samples and the traditional process. The difference of ethanol extract was not remarkable in all the samples.

CONCLUSIONThe study provide the feasibility of C. yanhusuo producing and concocting integration processing.

Acetic Acid ; chemistry ; Berberine Alkaloids ; analysis ; metabolism ; Corydalis ; chemistry ; metabolism ; Freeze Drying ; methods ; Plant Extracts ; analysis ; metabolism ; Plants, Medicinal ; chemistry ; Rhizome ; chemistry ; metabolism ; Solid Phase Extraction ; methods ; Water ; chemistry