Objective:To evaluate features and underlying pathogenesis of glucose metabolic disorder in pediatric patients with hyperthyroidism.Methods:The levels of blood glucose,insulin,c-peptide,glucagon,cortisol,T 3,T 4,TSH,TGA,TMA were measured in 29 patients with hyperthyroidism.20 normal age-matched children served as controls.In addition,fasting and post-prandial 60-minute erythrocyte insulin receptor of 14 children with hyperthyroidism and 7 normal age-matched controls were measured using insulin radioreceptor assay.Results:Incidence of glucose metabolic disorder was 34.5%.Incidence of glucose metabolic disorder in patients with more than one year course was higher compared with cases with less than one year course.In hyperthyroidism with glucose metabolic disorder,levels of glucose,insulin,insulin/glucose,insulin/glucagon at post-prandial 60 minute increased significantly.Numbers of fasting insulin receptor of high and low affinity in erythrocyte membrane were lower than post-prandial insulin receptor.Conclusion:Glucose metabolic disorder with impaired glucose tolerance and insulin resistance are present in patients with hyperthyroidism.The longer the course of the disease,the higher the incidence of glucose metabolic disorder.The decrease in the number of insulin receptor in target cells might play an important role in the mechanisms of glucose metabolic disorder and insulin resistance in children with hyperthyroidism.

OBJECTIVE: To observe the protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell (HUVEC) injury induced by oxidized low density lipoprotein (Ox-LDL) in vitro. METHODS: Ox-LDL was prepared and identified, and HUVECs were cultured. After 2-hour intervention of different drugs and 24-hour following intervention of Ox-LDL, the number of HUVECs was observed by phase contrast optical microscope and the activity of the HUVECs was observed by methyl thiazolyl tetrazolium (MTT) technique. Superoxide dismutase (SOD) activity and nitric oxide (NO) content were assayed by respective kit. The protein expressions and mRNA levels of peroxisome proliferators activated receptor gamma(PPAR-gamma) and endothelial nitric oxide synthase (eNOS) were measured by western blot technique and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Ox-LDL could increase the apoptosis rate of the HUVECs and decrease the NO release as compared with the blank control group (P<0.05). These effects induced by Ox-LDL were all significantly inhibited by Huaxia shallot preparation. It could up-regulate the protein expressions and mRNA levels of PPAR-gamma and eNOS significantly (P<0.05). Huaxia shallot preparation could decrease the apoptosis rate of the HUVECs. CONCLUSION: Ox-LDL may be involved in the initiation and progression of atherosclerosis by injuring the endothelial cells directly and may cause the endothelial dysfunction. Huaxia shallot preparation can protect against Ox-LDL induced endothelial cell injury by up-regulating the protein expressions and mRNA levels of PPAR-gamma and eNOS. It suggests that Huaxia shallot preparation may play a role in the prevention and treatment of cardiovascular disease.

OBJECTIVE:To evaluate clinical efficacy of Shenling baizhu powder combined with aminosalicylic acid in the treatment of ulcerative colitis (UC),and to provide evidence-based reference in clinic.METHODS:Retrieved from PubMed,Cochrane library,CJFD,VIP,Wanfang database and CBM,randomized controlled trials (RCTs) about Shenling baizhu powder combined with aminosalicylic acid preparations (trial group) vs.aminosalicylic acid preparations alone (control group) in the treatment of UC were collected.Meta-analysis was performed by using Rev Man 5.2 statistical software afder data extraction and quality evaluation with Cochrane systematic evaluator manual 5.1.0.RESULTS:A total of 14 RCTs were included,involving 1 177 patients.Meta-analysis showed that total response rate of trial group was significantly higher than that of control group [OR=3.40,95％ CI (2.44,4.74),P＜0.001],while Sutherland DAI score [MD=-1.31,95％ CI (-2.07,-0.56),P＜0.001],the levels of TNF-α [SMD=-1.90,95％CI(-2.90,-0.90),P＜0.001],IL-17[MD=-85.18,95％CI(-100.51,-69.85),P＜0.001] and IL-23 [MD=-113.27,95％CI(-132.34,-94.21),P＜0.001] in trial groups were significantly lower than control group,with statistical significance.CONCLUSIONS:Therapeutic efficacy of Shenling baizhu powder combined with aminosalicylic acid preparation is better than aminosalicylic acid preparation alone in the treatment of UC,and can significantly reduce Suthedand DAI and the levels of serum cytokines.

Objective To study the significance of serum hepatitis B virus large envelope protein (HBV-LP),HBV DNA and HBeAg in the diagnosis of viral replication.Methods In 623 chronic HBV patients,HBV-LP and HBeAg were detected by ELISA and HBV DNA was quantitaively detected by realtime polymerase chain reaction(PCR).Results The positive rate of HBV-LP and HBV DNA were higher than that of HBeAg in all chronic HBV patients(x2 =22.3 and 9.4,P ＜0.01),and the positive rate of HBVLP and HBV DNA was no significant difference (x2 =2.8,P ＞ 0.05),The positive rate of HBV-LP and HBV DNA detected in HBeAg(+) or HBeAg(-) were not significantly different(x2 =3.2 and 0.6,P ＞ 0.05).HBV DNA copies and A vaule of HBV-LP was a positive correlation(r =0.874,P ＜0.01),HBV DNA copies of different groups was significantly different from HBV-LP A values(F =6.987,P ＜0.01).In 100 healthy people,the detecting results of HBV DNA and HBV-LP are negetive.Conclusion HBV-LP was better than HBeAg for detection of HBV replication.There is a good correlation between the copies of HBV DNA and the levels of HBV-LP,HBV-LP expression can reflect the replication of HBV.

UNLABELLEDOBJECTIVE To investigate the effect of tea on oral cancer in nonsmokers and nondrinkers.

METHODSA case-control study were performed between September 2010 and January 2015 including 203 oral cancer cases in nonsmokers and nondrinkers with pathologically confirmed and 572 community controls. The related information included socio-demographic characteristics, detailed information on tobacco smoking and alcohol and tea consumption, personal medical history, family history of cancer, and occupational history were collected from all subjects. Unconditional logistic regression analysis was used to calculate the odds ratios (OR) and 95% confidence intervals (95% CI) to examine the effect of tea on oral cancer and to assess multiplicative interactions between tea and passive smoking. We also stratified by age, sex, residence, and passive smoking to explore possible difference in association between subgroups. Additive interactions between tea and passive smoking were assessed using relative excess risk due to interaction (RERI), attributable proportion (AP), and synergy index (SI).

RESULTSCompared with non-tea drinkers, tea consumption (OR = 0.52, 95% CI: 0.34-0.81), age of tea drinking initiation (years) ≥ 18 (OR = 0.54, 95% CI: 0.34-0.85), duration of tea consumption (years) < 20 (OR = 0.49, 95% CI: 0.27-0.90), duration of tea consumption (years) ≥ 20 (OR = 0.55, 95% CI: 0.32-0.95), average daily tea consumed < 700 ml (OR = 0.52, 95% CI: 0.32-0.86), moderate concentration of tea consumed (OR = 0.56, 95% CI: 0.32-0.96), weak concentration of tea consumed (OR = 0.35, 95% CI: 0.16-0.77), drinking green-tea (OR = 0.48, 95% CI: 0.28-0.82) and drinking moderate temperature of tea (OR = 0.55, 95% CI: 0.31-0.98) could reduce the risk of oral cancer; Stratified analysis indicated the protective effects of tea drinking on female (OR = 0.53, 95% CI: 0.30-0.94), age < 60 years old (OR = 0.53, 95% CI: 0.29-0.97), live in the urban (OR = 0.38, 95% CI: 0.20-0.69) and no passive smoking (OR = 0.47, 95% CI: 0.25-0.86) population with nonsmoking and nondrinking was more obvious; Crossover analysis showed tea and passive smoking did not exist multiplication interaction relationship (OR = 0.95, 95% CI: 0.41-2.20) and addition interaction relationship (RERI = -0.15, 95% CI: -0.92-0.62;AP = -0.16, 95% CI: -1.06-0.73; SI = -0.18, 95% CI: -1.44-0.87).

CONCLUSIONTea consumption, age of tea drinking initiation, duration of tea consumption, average daily tea consumed, concentration of tea consumed, types of tea and temperature of tea might have impact on the incidence of oral cancer in nonsmokers and nondrinkers to a certain extent.

Alcohol Drinking ; Case-Control Studies ; Female ; Humans ; Incidence ; Middle Aged ; Mouth Neoplasms ; epidemiology ; Odds Ratio ; Risk Factors ; Smoking ; Tea ; Temperature ; Tobacco Smoke Pollution

Objective To investigate the effect of tea on oral cancer in nonsmokers and nondrinkers. Methods A case-control study were performed between September 2010 and January 2015 including 203 oral cancer cases in nonsmokers and nondrinkers with pathologically confirmed and 572 community controls. The related information included socio-demographic characteristics, detailed information on tobacco smoking and alcohol and tea consumption, personal medical history, family history of cancer, and occupational history were collected from all subjects. Unconditional logistic regression analysis was used to calculate the odds ratios (OR) and 95%confidence intervals (95%CI) to examine the effect of tea on oral cancer and to assess multiplicative interactions between tea and passive smoking. We also stratified by age, sex, residence, and passive smoking to explore possible difference in association between subgroups. Additive interactions between tea and passive smoking were assessed using relative excess risk due to interaction (RERI), attributable proportion (AP), and synergy index (SI). Results Compared with non-tea drinkers, tea consumption (OR=0.52, 95%CI:0.34-0.81), age of tea drinking initiation (years)≥18 (OR=0.54, 95%CI: 0.34-0.85), duration of tea consumption (years) <20 (OR=0.49, 95%CI: 0.27-0.90), duration of tea consumption (years)≥20(OR=0.55, 95%CI:0.32-0.95), average daily tea consumed<700 ml(OR=0.52,95%CI:0.32-0.86), moderate concentration of tea consumed (OR=0.56,95%CI:0.32-0.96), weak concentration of tea consumed(OR=0.35, 95%CI: 0.16-0.77), drinking green-tea(OR=0.48,95%CI: 0.28-0.82) and drinking moderate temperature of tea (OR=0.55,95%CI: 0.31-0.98) could reduce the risk of oral cancer; Stratified analysis indicated the protective effects of tea drinking on female (OR=0.53,95%CI:0.30-0.94), age<60 years old (OR=0.53,95%CI:0.29-0.97), live in the urban(OR=0.38,95%CI:0.20-0.69) and no passive smoking(OR=0.47,95%CI:0.25-0.86) population with nonsmoking and nondrinking was more obvious; Crossover analysis showed tea and passive smoking did not exist multiplication interaction relationship (OR=0.95,95%CI:0.41-2.20) and addition interaction relationship (RERI=-0.15,95%CI:-0.92-0.62;AP=-0.16,95%CI:-1.06-0.73;SI=-0.18, 95%CI:-1.44-0.87). Conclusion Tea consumption, age of tea drinking initiation, duration of tea consumption, average daily tea consumed, concentration of tea consumed, types of tea and temperature of tea might have impact on the incidence of oral cancer in nonsmokers and nondrinkers to a certain extent.

Objective To investigate the association between oral hygiene, chronic diseases, and oral squamous cell carcinoma. Methods We performed a case-control study with 414 cases and 870 controls in Fujian during September 2010 to January 2015. Patients were newly diagnosed oral squamous cell carcinoma cases according to the pathologic diagnoses, control subjects were enrolled from community population. Epidemiological data were collected by in-person interviews using a standard questionnaire . The contents of the questionnaire included demography character, history of tobacco smoking and alcohol drinking, dietary habits, oral hygiene status, family history of cancer, etc. Using unconditional logistic regression analysis to estimate adjusted odds ratios (OR) and corresponding 95% confidence intervals (CI) for oral hygiene and chronic diseases. We also stratified by sex, smoking and drinking to explore possible difference in association between subgroups. Results The multivariate logistic regression analysis indicated that number of teeth(20-27 and<20), bad prosthesis, recurrent oral ulceration were the risk factors of oral squamous cell carcinoma, the adjusted OR(95%CI) values were 2.01(1.49-2.73), 3.51(2.39-5.15), 2.33(1.79-3.04), 3.96(2.11-7.44), respectively;brushing tooth once per bay,brushing tooth more than once per day, regular oral health examination at least 5 years per time were the protective factors of oral squamous cell carcinoma, the adjusted OR(95%CI) values were 0.24 (0.13-0.43),0.13 (0.07-0.24), 0.37 (0.26-0.53), respectively. The stratification analysis indicated that recurrent oral ulceration could increase the risk of oral squamous cell carcinoma for non-smokers and non-drinking, the adjusted OR(95%CI) value was 5.21(2.42-11.18) and 4.71(2.37-9.36);and a risky effect of hypertension on risk of oral squamous cell carcinoma was observed for non-smokers and non-drinking, the adjusted OR(95%CI) values were 1.70 (1.10-2.61) and 1.58 (1.07-2.34) . Conclusions Oral hygiene and chronic diseases could affect the incidence of oral squamous cell carcinoma.

Objective To investigate the clinical influence factors of oral-maxillofacial benign tumors. Methods We conducted a case-control study with 113 cases newly diagnosed primary oral-maxillofacial benign tumors and 584 cases controls from a hospital in Fujian from September 2010 to January 2015. Epidemiological data were collected by in-person interviews using a standard questionnaire. The contents of the questionnaire included demography character, history of tobacco smoking and alcohol drinking, dietary habits, oral hygiene status, family history of cancer, etc. Unconditional logistic regression was used to research the relationship between the factors and oral-maxillofacial benign tumors. Results Multivariable analysis showed that risk factors of oral-maxillofacial benign tumors included: cigarette smoking index above 1 000, passive smoking before the age of 18, age of wearing bad prosthesis between 33 to 55 years old and high blood pressure; the corresponding OR(95%CI) values were 14.63 (3.88-55.13), 2.34 (1.19-4.62), 2.35 (1.17-4.73), 3.46(1.71-7.00), respectively; Protective factors included: regularly intake of meat above 1 time/day, fruits, health care products and vitamin tablets, brushing teeth above 1 time per day and oral examination above 5 years/time, the corresponding OR(95%CI) values were 0.22 (0.07-0.70), 0.18 (0.08- 0.41), 0.32 (0.11- 0.88), 0.22 (0.07- 0.73), 0.28 (0.16-0.48), 0.28 (0.13-0.60), respectively. Conclusion Abstinence from tobacco smoking, reduce passive smoking before the age of 18, regularly intake of meat, fruits, health care products and vitamin tablets, and oral examination at regular time might have impact on the incidence of oral-maxillofacial benign tumors to a certain extent.

Objective To investigate the effect of tea on oral cancer in nonsmokers and nondrinkers. Methods A case-control study were performed between September 2010 and January 2015 including 203 oral cancer cases in nonsmokers and nondrinkers with pathologically confirmed and 572 community controls. The related information included socio-demographic characteristics, detailed information on tobacco smoking and alcohol and tea consumption, personal medical history, family history of cancer, and occupational history were collected from all subjects. Unconditional logistic regression analysis was used to calculate the odds ratios (OR) and 95%confidence intervals (95%CI) to examine the effect of tea on oral cancer and to assess multiplicative interactions between tea and passive smoking. We also stratified by age, sex, residence, and passive smoking to explore possible difference in association between subgroups. Additive interactions between tea and passive smoking were assessed using relative excess risk due to interaction (RERI), attributable proportion (AP), and synergy index (SI). Results Compared with non-tea drinkers, tea consumption (OR=0.52, 95%CI:0.34-0.81), age of tea drinking initiation (years)≥18 (OR=0.54, 95%CI: 0.34-0.85), duration of tea consumption (years) <20 (OR=0.49, 95%CI: 0.27-0.90), duration of tea consumption (years)≥20(OR=0.55, 95%CI:0.32-0.95), average daily tea consumed<700 ml(OR=0.52,95%CI:0.32-0.86), moderate concentration of tea consumed (OR=0.56,95%CI:0.32-0.96), weak concentration of tea consumed(OR=0.35, 95%CI: 0.16-0.77), drinking green-tea(OR=0.48,95%CI: 0.28-0.82) and drinking moderate temperature of tea (OR=0.55,95%CI: 0.31-0.98) could reduce the risk of oral cancer; Stratified analysis indicated the protective effects of tea drinking on female (OR=0.53,95%CI:0.30-0.94), age<60 years old (OR=0.53,95%CI:0.29-0.97), live in the urban(OR=0.38,95%CI:0.20-0.69) and no passive smoking(OR=0.47,95%CI:0.25-0.86) population with nonsmoking and nondrinking was more obvious; Crossover analysis showed tea and passive smoking did not exist multiplication interaction relationship (OR=0.95,95%CI:0.41-2.20) and addition interaction relationship (RERI=-0.15,95%CI:-0.92-0.62;AP=-0.16,95%CI:-1.06-0.73;SI=-0.18, 95%CI:-1.44-0.87). Conclusion Tea consumption, age of tea drinking initiation, duration of tea consumption, average daily tea consumed, concentration of tea consumed, types of tea and temperature of tea might have impact on the incidence of oral cancer in nonsmokers and nondrinkers to a certain extent.

Objective To investigate the association between oral hygiene, chronic diseases, and oral squamous cell carcinoma. Methods We performed a case-control study with 414 cases and 870 controls in Fujian during September 2010 to January 2015. Patients were newly diagnosed oral squamous cell carcinoma cases according to the pathologic diagnoses, control subjects were enrolled from community population. Epidemiological data were collected by in-person interviews using a standard questionnaire . The contents of the questionnaire included demography character, history of tobacco smoking and alcohol drinking, dietary habits, oral hygiene status, family history of cancer, etc. Using unconditional logistic regression analysis to estimate adjusted odds ratios (OR) and corresponding 95% confidence intervals (CI) for oral hygiene and chronic diseases. We also stratified by sex, smoking and drinking to explore possible difference in association between subgroups. Results The multivariate logistic regression analysis indicated that number of teeth(20-27 and<20), bad prosthesis, recurrent oral ulceration were the risk factors of oral squamous cell carcinoma, the adjusted OR(95%CI) values were 2.01(1.49-2.73), 3.51(2.39-5.15), 2.33(1.79-3.04), 3.96(2.11-7.44), respectively;brushing tooth once per bay,brushing tooth more than once per day, regular oral health examination at least 5 years per time were the protective factors of oral squamous cell carcinoma, the adjusted OR(95%CI) values were 0.24 (0.13-0.43),0.13 (0.07-0.24), 0.37 (0.26-0.53), respectively. The stratification analysis indicated that recurrent oral ulceration could increase the risk of oral squamous cell carcinoma for non-smokers and non-drinking, the adjusted OR(95%CI) value was 5.21(2.42-11.18) and 4.71(2.37-9.36);and a risky effect of hypertension on risk of oral squamous cell carcinoma was observed for non-smokers and non-drinking, the adjusted OR(95%CI) values were 1.70 (1.10-2.61) and 1.58 (1.07-2.34) . Conclusions Oral hygiene and chronic diseases could affect the incidence of oral squamous cell carcinoma.