Objective To study the curative effect of accessory breast resection with axillary minidermato-glyph incision.Methods The clinical data of 47 patients undergoing accessory breast operation in our hospital be-tween April 2010 and June 2014 were retrospectively analyzed according to different methods of operation.They were divided into two groups:26 cases with axillary minidermatoglyph incision as the observation group and 21 cases with conventional accessory breast resection as the control group.The postoperative wound healing and incidence of com-plications were then compared.All the patients were followed up for 6 months.Self-designed questionnaire of visual simulation effects were adopted and shape, local pain and upper extremity activities were evaluated by the patients. Results The two groups had no significant difference in the unilateral average operation time (p >0.05).The bleeding volume in the observation group was less than the control group, and the average length of the incision and the average hospital stay were significantly shorter than those of the control group (p<0.05).After 6 months of fol-low-up, the basic satisfaction rate of shape of patients in the observation group was 95.15%, upper extremity activi-ty reached 100%, and the rate of local painlessness was 95.15%, both higher than the control group (p<0.05). Conclusion Accessory breast resection with axillary minidermatoglyph incision has the advantages of reliable cura-tive effect, less damage, inconspicuous local scar and no significant effect on physical activity.

Objective To explore the effects and mechanisms of periostin overexpression on migration and invasion of nasopharyngeal carcinoma ( NPC) cell line.Methods The recombinant plasmids [ pCMV-neo ( +)-periostin ] and control plasmids [pCMV-neo (+)] were transfected into 6-10B cells using lipofectamine 2000TM reagent.The expression of periostin was detected with PCR and Western blotting .Transwell chamber invasion assay was employed to assay the migration and invasion of 6-10B cells before and after transfection .A gelatin zymogram was used to detect the activity of MMP-2 and MMP-9 in cultivated supernatant of 6-10B cells before and after transfection .The expression of integrin-αvβ5 was detected by immunohistochemistry ( IHC) in 6-10Bperiostin cells, 6-10Bvector cells and 6-10B cells as well as normal nasopharyngeal mucosa ( NNM) and NPC and at the same time periostin also was detected by immumohistochemistry in NNM and NPC, and densitometry analysis using image-pro plus 6.0 software, and the correlation between periostin and integrin-αvβ5 on NPC was assayed with statistics .Results Over expression of periostin promoted cell migration and invasion.The expression levels of integrin-αvβ5 in primary NPC and 6-10Bperiostin cells were significantly higher than those in NNM and 6-10Bvector, 6-10B cells.The expression in NPC of integrin-αvβ5 showed positively correlated with the expression of periostin (r=0.682, P<0.01).Conclusion Periostin plays an important role in regulation of cell migration and invasion probably by combining with integrin-αvβ5 to improve the activities of MMPs .

Lower extremity deep vein thrombosis (DVT) is one of the main complications in patients with traumatic fractures, and for severe patients, the DVT can even affect arterial blood supply, resulting in insufficient limb blood supply. If the thrombus breaks off, pulmonary embolism may occur, with a high mortality. The treatment and rehabilitation strategies of thrombosis in patients with lower extremity fractures have its particularity. DVT in traumatic fractures patients has attracted extensive attention and been largely studied, and the measures for prevention and treatment of DVT are constantly developing. In recent years, a series of thrombosis prevention and treatment guidelines have been updated at home and abroad, but there are still many doubts about the prevention and treatment of DVT in patients with different traumatic fractures. Accordingly, on the basis of summarizing the latest evidence-based medical evidence at home and abroad and the clinical experience of the majority of experts, the authors summarize the clinical treatment and prevention protocols for DVT in patients with traumatic fractures, and make this consensus on the examination and assessment, treatment, prevention and preventive measures for DVT in patients with different fractures so as to provide a practicable approach suitable for China ′s national conditions and improve the prognosis and the life quality of patients.