Objective Proteinuria is in close relation with arteriosclerosis,but little investigation was made on the correlation between ankle-brachial index( ABI) and proteinuria in patients with acute cerebral infarction. Methods 271 consecutive patients with acute cerebral infarction were enrolled from January 2013 and December 2013, which were divided into 2 groups ( patients with proteinuria and patients without proteinuria) according to urine analysis.ABI of all patients was measured and stratified into 4 groups:ABI≤0.9 (low);0.9

Objective To analyze the resistance of Stenotrophomonas maltophilia to commonly used antibacterial drugs,and to investigate the existence status of 3 kinds of integron(Ⅰ,Ⅱand Ⅲ)and the carrying drug resistance gene cassettes so as to offer help for better preventing and controlling the infectious diseases caused by stenotrophomonas maltophilia in local place.Methods 51 strains of Stenotrophomonas maltophilia from clinical samples were collected and the minimum inhibitory concentration(MIC)of 17 kinds of antibacterial drugs to stenotrophomonas maltophilia was measured by the broth microdilution antifungal susceptibility test(Mi-AFST).3 kinds of integron(Ⅰ,Ⅱand Ⅲ)were amplified by PCR with primers designed according to registrated DNA se-quence in GenBank.The variable region products in the positive integron strains were amplified and performed the sequencing analy-sis.The homological comparison in the Genebank database was performed on the sequencing results for finding out what gene was included in variable domain of those integrons.Results (1)Among 51 strains of stenotrophomonas maltophilia,41 srains (80.39%) were collected from sputum samples,and the infected crowd was dominated by individuals aged over 60 years,38 strains accounted for 74.5%.In the department distribution,20 strains(39.22%)were collected from ICU,13 strains (25.49%)from the respiratory department and 6 strains(11 .76%)from the veteran cardres wards,which accounted for the larger proportion.(2)The drug suscep-tibility test demonstrated that stenotrophomonas maltophilia strains had the higher resistance to most of commonly used antibacteri-al drugs,some strains even showed the multi-drug resistance to over 9 kinds of antibacterial drugs.(3)The PCR gene amplification results showed that 7 strains (13.7%)were positive integronⅠ,while no strains containing integron Ⅱ or ⅢI were detected;the resistance genes carried in the variable region of integron I included the 5 kinds of aacA4,aadA1,catB8,dfrA17 and aphA15.Conclu-sion Stenotrophomonas maltophilias has relatively high resistance to majority of commonly used antibacterial drugs in clinic,and some strains show the muti-drug resitance.IntegronⅠis one of important factors for the multi-drug resistance of Stenotrophomonas maltophilias.

OBJECTIVE To investigate the antimicrobial susceptibility of Streptococcus pneumoniae isolated in Yantai and their mechanisms of resistance to macrolides.METHODS Antimicrobial susceptibility of S.pneumoniae was determined by agar dilution method.Phenotypes of macrolide-resistant S.pneumoniae were determined using double disk test with erythromycin and clindamycin disks.ermB And mefE genes were amplified by PCR.RESULTS Among 42 strains of S.pneumoniae,65.0% were intermediate to and no strain was resistant to penicillin.The resistance rates to erythromycin and clindamycin were 93.0%,respectively.Of 41 erythromycin resistantstrains,93.0% were constitutive resistant.ermB Was detected in 40 strains and mefE in 1 strain,both ermB and mefE genes were found in 9 strains.CONCLUSIONS The resistance rate of S.pneumoniae to penicillin is high in Yantai area,the resistance rates to erythromycin and clindamycin are very high.Target modification by ermB methylase is the predominant mechanism in macrolide-resistant S.pneumoniae in Yantai.

Objective To explore the effects and mechanisms of periostin overexpression on migration and invasion of nasopharyngeal carcinoma ( NPC) cell line.Methods The recombinant plasmids [ pCMV-neo ( +)-periostin ] and control plasmids [pCMV-neo (+)] were transfected into 6-10B cells using lipofectamine 2000TM reagent.The expression of periostin was detected with PCR and Western blotting .Transwell chamber invasion assay was employed to assay the migration and invasion of 6-10B cells before and after transfection .A gelatin zymogram was used to detect the activity of MMP-2 and MMP-9 in cultivated supernatant of 6-10B cells before and after transfection .The expression of integrin-αvβ5 was detected by immunohistochemistry ( IHC) in 6-10Bperiostin cells, 6-10Bvector cells and 6-10B cells as well as normal nasopharyngeal mucosa ( NNM) and NPC and at the same time periostin also was detected by immumohistochemistry in NNM and NPC, and densitometry analysis using image-pro plus 6.0 software, and the correlation between periostin and integrin-αvβ5 on NPC was assayed with statistics .Results Over expression of periostin promoted cell migration and invasion.The expression levels of integrin-αvβ5 in primary NPC and 6-10Bperiostin cells were significantly higher than those in NNM and 6-10Bvector, 6-10B cells.The expression in NPC of integrin-αvβ5 showed positively correlated with the expression of periostin (r=0.682, P<0.01).Conclusion Periostin plays an important role in regulation of cell migration and invasion probably by combining with integrin-αvβ5 to improve the activities of MMPs .