1.Isolation and identification of endophytes from Pinellia.
Jianling LIU ; Baobao CHEN ; Yonghong LIU ; Jiangbin ZHENG ; Zongsuo LIANG
China Journal of Chinese Materia Medica 2009;34(18):2305-2307
OBJECTIVETo investigate the varieties of entophytes in different parts of Pinellia ternata.
METHODThe solidified plates were applied for the isolation of the endophytes, and three methods were used for the identification of endophytic fungi.
RESULTEighty four strains of the entophytes were isolated from the P. ternata collected from 3 habitations. Endophytic fungi were morphologically identified belonging to 15 genera, 4 families.
CONCLUSIONIt indicated that the entophytes in P. ternata were diversity and rich, and there were some differences at quantity and species in different organs of P. ternata.
Biodiversity ; Fungi ; classification ; isolation & purification ; physiology ; Phylogeny ; Pinellia ; microbiology ; physiology ; Symbiosis
2.Study on the longevity related mitochondrial genome variation in Bama elderly population in Guangxi province.
Ze-ping LV ; Chen-guang ZHENG ; Fang KONG ; Jie FENG ; Wen-yu JIANG ; Cai-you HU ; Hua LI ; Yuan LV ; Gen-fa ZHANG ; Ze YANG
Chinese Journal of Medical Genetics 2010;27(4):423-427
OBJECTIVETo investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi.
METHODSMitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism (SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi.
RESULTSThe 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls. And it was significantly higher in controls than that in long-lived population (P<0.05).
CONCLUSIONThe mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.
Aged ; China ; ethnology ; DNA, Mitochondrial ; analysis ; genetics ; Genome, Mitochondrial ; genetics ; Haplotypes ; Humans ; Longevity ; genetics ; Mutation ; Myanmar ; ethnology ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; genetics ; Population Groups