Objective:To investigate the protection of acupuncture on damaged myelin sheath from the poinr of view of neurological functional recovery and morphological changes of the myelin sheath.Methods:Eighty-nine adult male Sprague-Dawley rats were randomly allocated to normal (n=5),sham-operation (n=21),model (n=21),early acupuncture (n=21) and late acupancture (n=21) groups.A model of middle cerebral artery occlusion was induced by the intraluminal filament method.Acupuncture was performed at different time points after ischemia.Neurological function and morphological changes of the myelin sheath of ischemic focus were observed by using the methods of neurological deficit scores and Pal-Weigert's myelin staining.Results:Neurological deficit scores at 2.5 hours after the procedure in the model group increased significantly (P＜0.05).qhe scores decreased somewhat as the time elapsed.The internal capsule had obvious demyelination and recovered slowly.Neurological deficit scores in rats at each time point decreased faster in the acupuncture group compared with the model group,and they decreased significantly at day 3 (P＜0.05);the extent of demyelination was significantly alleviated.Neurological deficit scores decreased faster in the early acupuncture group compared with the late acupuncture group (P＜0.05);and demyelination in the early acupuncture group seemed milder at day one.Conclusions:Early acupuncture is beneficial to remyelination and neurological functional recovery after cerebral ischemia.

The differentiating of neurons and other distinct cell types during embryonic development requires the selective activation or repressing of many different sets of genes. Gene expression patterns in neurons are modulated by multiple extracellular and intracellular stimuli. The transcriptional regulation of individual gene is mediated by small DNA sequences such as silencer and enhancer, and the expression pattern can be determined by the integration of the effects of a very large number of these cis-acting elements. These DNA elements either activate or repress promoter activity depending upon the nature of the transcription factors that bind to them. It is possible that there are different regulatory mechanisms of gene expression in the nerve system.

With the aggravation of aging of the population,the incidence of Alzheimer's disease (AD)has been steadily increasing,mild cognitive impairment (MCI)is considered as a cognitive state between aging and dementia.However,the diagnosis of AD and MCI is difficult and mainly depends on the clinical symptoms and the corresponding assessment scales,lacking of the application of biological markers.In this paper,we presented a general review of the biological markers related to AD and MCI,so as to provide reference for their clinical use.

Objective To observe the effect of Donglian capsule on nonenzymadc protein glycation.Methods An animal model of DM was established by injecting a little dosage of STZ in high fat-fed rats.When the models were established successfully,corresponding medicines were supplied for 12 weeks and then the blood glucose,glycosylation serum protein(GSP)and aldose reductase(AR)in rats semm were measured.Results Comparing with the model group,the levels of blood glucose,GSP and AR in Metformin Hydrochloride tablets group and Chinese medicine group were decreased markedly (P＜0.05).Conclusion Donglian capsule Can restrain nonenzymatic protein glycation and so thus prevent and treat DM.

AIM:To investigate the effect of chronic unpredictable mild stress(CUMS) on plasma endothelin,nitric oxide and interleukin-6,high-sensitivity C-reactive protein(hs-CRP) in rats after acute myocardial infarction(AMI).METHODS:After the rats of myocardial infarction(MI) were established,the animals were treated under the condition of CUMS for 4 weeks,then the contents of plasma(or serum) endothelin,nitric oxide,interleukin-6 and hs-CRP were measured and data were analyzed by two factor factorial analysis. RESULTS:The results of factorial analysis showed:MI alone had no significantly effect on the level of nitric oxide(P>0.05),but CUMS had significantly effect on increasing the level of nitric oxide(P<0.01). The CUMS had significantly interaction with MI on increasing the level of nitric oxide(P<0.01). Both MI and CUMS increased the level of hs-CRP(all P<0.01). However,no interaction was discovered between MI and CUMS(P>0.05). Both MI and CUMS had no effect on the level of ET-1 and IL-6(P>0.05).CONCLUSION:CUMS increases nitric oxide content and has cooperative effect with MI on increasing NO,both MI and CUMS significantly increase the level of CRP,but have no effect on the level of ET-1 or IL-6,suggesting that the abnormal increase in nitric oxide and hs-CRP contents may be the important pathophysiological changes of post-MI depression.

Hydroxytyrosol ( 3 , 4-dihydroxyphenyle-thanol) presents in olive leaves and fruits with an ex-ceptional antioxidative activity. As a promising func-tional food ingredient, hydroxytyrosol shows many bio-logical activities, including preventing cardiovascular diseases, protecting retinal pigment epithelial cells, preventing osteoporosis, and improving the cognitive a-bility. According to the research progress in recent years,we have summarized the biological effect and the metabolism of hydroxytyrosol in this review.

Alzheimer's disease (AD) is a neurodegenerative disease of the central nervous system, and its pathogenesis is very complex.As a large number of microorganisms in the human digestive tract, intestinal flora is the important environmental factor in the process of human health and disease.The brain-gut axis is a bidirectional information regulation system which connects the brain and the gastrointestinal.The intestinal microbes can participate in the brain-gut axis activity, and influence the brain function and some related behaviors under physiological and pathological conditions.Recent studies have shown that intestinal microorganisms are closely related to AD, and they interact with each other through immune, endocrine and vagal pathways to form complex networks.Many reports have shown that probiotics or traditional Chinese medicine can regulate the state of intestinal microflora and improve AD symptoms.Therefore, in order to provide a new research idea and method for the clinical prevention and treatment of AD, it is necessary to further study the interaction between intestinal microbes, traditional Chinese medicine and AD.

Objective To explore the imaging and pathological characteristics of the telangiectatic osteosarcoma.Methods The clinical,imaging and pathological data of telangiectatic osteosareoma in 12 cases were analysed.Results The tumors often located at the diaphysialepiphysial of the os longum and most of them were occured at the lower limb,increasing of the alkaline phosphatase(AKP) was rare.X-ray and CT character istics were osteoclastic and expensive bony destruction;there were multiple small cystic formation and fluid-fluid level in the destructive region and soft masess;the cortex of the bone became thinner with multiple sieve type destruction;the periosteal reaction and radial bone spicule were often seen.Pathological characteristics were of typical tumor cell of the osteosarcoma,mutliple sinus of the blood and "color bland shape" component.Conclusion The telangiectatic osteosarcoma has certain specific clinical,imaging and histologyical characteristics.

Objective To investigate the effect of carbon dioxid insufflation on cerebral blood flow (CBF) during laparoscopic cholecystectomy. Methods Thirty patients ASA grade Ⅰ- Ⅱ undergoing laparoscopic cholecystectomy were selected. The values of jugular venous oxygen partial pressure(PjvO2 ) jugular venous oxygen saturation (SjvO2 ), arterial partial pressure of carbon dioxide (PaCO2), oxygen (PaO2) and saturation (SaO2 ) were measured before the insufflation, 10 min and 30 min after the insufnation, respectively. Results Compared with those before the insufflation, SjvO2 and PacO2 were increased significantly, and arterial--jugular venous oxygen content difference (Ca-jvDO2 ) decreased markedly 10 min and 30 min after insufflation (P

Objective:To study intervenient action of Qixue Bingzhi Prescription on the patient of cervical atherosclerosis(AS). Methods:63 cases of cervical AS were randomly divided into a treatment group(n=33) and a control group(n=30), with 25 cases of same age ranges used as healthy group. The control group was treated with basic treatment of western medicine and the treatment group with the basic treatment plus Qixue Bingzhi Prescription for one month. Changes of blood lipid metabolism, interleukin levels, supersensitive C-reactive protein(Hs-CRP) content and the leukocyte adherence expression rate were observed. Results: The total cholesterol (TC), triglyceride(TG), AS index (TC/HDL-C), interleukin-6 (IL-6), interleukin-8 (IL-8) and Hs-CRP levels and lymphocytes and the neutrophilic granulocyte adhesion molecule expression rate (ICD11b and zCD11b) decreased significantly(P 0. 05), Conclusion: Qixue Bingzhi Prescription can effectively and safely intervene in blood lipid metabolism and inflammatory reaction in the patient of cervical AS, and it has prospect of development in preventing formation of AS and stabilizing plaque.