Objective:To explore the necessity of monitoring polysomnography (PSG ) in snoring children.Method:The PSG were monitored in 162 children with snoring or apnea.Acording to results of PSG,162 cases were separated into two groups:group obstructive sleep apnea hypopnea syndrome(OSAHS) and group primary snoring(PS),monitoring data including:apnea hypopnea index(AHI),snoring index(SI),lowest oxygen saturation(LSaO_2) and time of SaO_2<0.9,all data were undertaked statistical analysis. Result:In all 162 snoring cases,150 cases(92.6%) belong to group OSAHS,12 cases(7.4%) belong to group PS;Differences of data of age,sex and body mass index(BMI) are not significant but that of AHI,SI,LSaO_2 and time of SaO_2<0.9 are significant(P<0.05).Conclusion:It's necessary to monitor PSG in snoring children for early diagnosis and correct treatment to avoid disadvantage of OSAHS.

Orthokeratology is a kind of rigid contact lens which have reverse geometric desi, with higher oxygen permeability and security.Overnight wearing of orthokeratology can decrease the central corneal curvature and increase peripheral corneal curvature by flatting the central department of corneal, thus reduce the refraction of myopia.Through a period of time of wearing orthokeratology lens, patients can obtain good eyesight without frame glasses.Insisting on wearing orthokeratology lens can control the development of myopia.Orthokeratology is widely applied in the control of juvenile myopia, so we need scientific evaluation system to measure the visual quality after wearing orthokeratology lens.Here are the methods that used to evaluate the visual quality after wearing orthokeratology lens.

AIM:To evaluate the effect of rigid gas permeable ( RGP ) lens on the tear film and the visual quality in adolescents with a double-pass system.
METHODS:This was a prospective study comprised 23 myopia patients ( 39 eyes ) wearing rigid gas permeable contact lens ( RGP ) between Jun. 2015 and Aug. 2015 in the Department of Ophthalmology of Jinling Hospital. Uncorrected visual acuity ( UCVA ) , sphere refractive, cylinder refractive, Schirmer I test, tear film break-up time ( BUT ) , the visual quality and the tear film quality with OQAS II were obtained before and 1wk, 1, 3, 6mo after wearing. The data was analyzed using t-test and One-way.
RESULTS:The UCVA in before and after 1wk, 1, 3, 6mo were 0. 23±0. 10, 0. 81±0. 23, 0. 99±0. 11, 1. 01±0. 09, 0. 95±0. 14, the difference was statistically significant ( F=723. 36, P<0.01). The sphere in before and after 1wk, 1, 3, 6mo were (-2. 83±1. 34) D, (-0. 63±0. 82) D, (-0. 12±0. 20) D, (-0. 03±0. 10 ) D, (-0. 10±0. 30 ) D respectively, the difference was statistically significant (F=107. 01, P<0. 01). The refractive cylinder in before and after 1wk, 1, 3, 6mo were (-0. 12±0. 21) D, (-0. 13±0. 22) D, (-0. 12±0. 21) D, (-0. 14±0. 26) D, (-0. 21±0. 27) D respectively, there was no significant difference before and after wearing RGP (F=2. 58, P>0. 05). Schirmer I test showed no statistically significant difference before and after wearing RGP ( F=4.88, P>0. 05). The stability of tear film was reduced after wearing RGP, and the difference was statistically significant (F=135. 11, P<0. 01). The modulate transfer function ( MTF ) cut- off frequency was reduced after wearing RGP, the object scatter index ( OSI ) was increased after wearing RGP, and the difference was statistically significant, the tear film quality OSI was increased after wearing RGP, and the difference was statistically significant (F=6. 16, 19. 23, 10. 62, P<0. 01).CONCLUSION:The UCVA is significantly increased after wearing RGP. However, there was no significant change in the base-line of tear secretion, but BUT is significantly reduced after wearing RGP. The stability of the tear film is reduced, which can lead to a decline in visual quality.

Objective To explore whether theophylline has clinic effects of treatment on bronchiolitis in children. Methods 128 children with bronchiolitis in our hospital were divided into the experiment and the control at random, in the meantime, grouped into the severe and the mild according to their condition. The children in the control were used traditional methods. The children in the control were taken theophylline, as well as used traditional methods. All patients in our hospital were checked up pulmonary function, alkyladenine glycosylase in trachea secretion, serum interleukin 8, cytotaxonomy in trachea secretion on time, after the third day and the 7th day. Results The pulmonary function and cytotaxonomy of the patients in the experiment were more improvement than that of the control. Their alkyladeniue gtycosylase in trachea secretion and serum interleukin 8 were very lower than that of the control. The cure rate in the mild and the severe of the experi-ment were higher than that of the control. Conclusion Administrating theophyUine by oral can improve the pulmonary function, relax a block of the small trachea, and raise the cure rate of patients with bronchiolitis.

Adult ; Diagnosis, Differential ; Dyspepsia ; blood ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Motilin ; blood

Objective:To examine the adiponectin level in gingival crevicular fluid(GCF)in patients of chronic periodontitis with dia-betes mellitus type 2.Methods:20 patients of diabetes mellitus type 2 with chronic periodontitis(DM&CP),20 of periodontitis(CP) and 20 health subjects(H)were included.The periodontal indexes (SBI,PLI,PD and AL)were measured,GCF samples were quan-tified by periotron 8000,the adiponectin content in GCF was tested by adiponectin ELISA kit.The relationship between the adiponectin level in GCF and the periodontal indexes of the DM&CP patients was analyzed statistically.Results:The adiponectin level in GCF in group DM&CP was significantly lower than that in the other 2 groups(P <0.05).The adiponectin levels in GCF in group CP and H were not statistically different.The adiponectin level in GCF was negatively correlated with PD and AL(P <0.05),but had no correlation with SBI and PLI(P >0.05).Conclusion:Decrease of adiponectin in GCF may play a role in the development of DM&CP.

A total of 12 775 SSRs were identified from Scutellaria baicalensis Georgi genomic database, accounting for 2.56% of the total genomic sequences. The result showed that S. baicalensis SSRs were based on 68.32% dinucleotide and 18.63% trinucleotide repeats; CT/GA and TTC/GAA were predominant in the dinucleotide motifs and the trinucleotide motifs respectively. Nine primers were selected to produce highly reproducible SSR bands and were used in studying the genetic diversity of S. baicalensis, 50 individuals from ten populations. 68 SSR polymorphic loci were detected, these loci were polymorphic and displayed 4 to 12 alleles per locus with a mean number of 7; the effect number of alleles was 3. Expected heterozygosities were 0.6 and were far more greater than the average in dicotyledonous plants. PIC (polymorphism information content) was 0.72, Shannon's information index was 1.32, these all proved that S. baicalensis had a high genetic diversity in general. Genetic differentiation among population Gst was 0.131, genetic variation among population accounted for 13.1% and genetic variation within population accounted for 86.9%. The cluster analysis showed that 10 populations S. Baicalensis were classified into 2 groups, but it was not associated with geographical distribution.

Aim To investigate the effect of CD44 anti-body-A3 D8 on the expression of IL-3 Rα and down-stream PI3K/Akt in NB4 cells. Methods The ex-pression of IL-3 Rα mRNA was detected by real-time quantitative RT-PCR, the IL-3Rα protein expression and changes of PI3 K/Akt signal pathway in NB4 cells treated with A3D8 were analyzed by Western blot. An-nexin-V-FITC/PI double staining flow cytometry was u-tilized to detect the apoptotic cells. The inhibitor of PI3 K/Akt signaling LY294002 combined with A3 D8 was used to inhibit the PI3K/Akt in NB4 cells. Re-sults After treated with A3 D8 , both the transcription-al level and translational level of IL-3 Rα were remark-ably reduced, and the PI3K/Akt pathway was inhibi-ted. LY294002 improved the inhibitory and apoptotic effects of A3D8 on NB4 cells. Conclusion CD44 antibody A3 D8 can downregulate the expression of IL-3Rα and inhibit the downstream PI3K/Akt pathway.

Recent studies reported that thymoquinone (TQ), a component derived from the medicinal spice Nigella sativa (also called black cumin), exhibited inhibitory effects on cell proliferation of many cancer cell lines. This study was performed to investigate the anti-metastatic effect of thymoquinone on the pancreatic cancer in vitro and in vivo. The results showed that thymoquinone suppressed the migration and invasion of Panc-1 cells in a does-dependent manner. To investigate the possible mechanisms involved in these events, Western blotting analysis was performed, and found that thymoquinone significantly down-regulates NF-kappaB and MMP-9 in Panc-1 cells. In addition, metastatic model simulating human pancreatic cancer was established by orthotropic implantation of histologically intact pancreatic tumor tissue into the pancreatic wall of nude mice. And administration of thymoquinone significantly reduced tumor metastasis compared to untreated control. Furthermore, the expression of NF-kappaB and MMP-9 in tumor tissues was also suppressed after treatment with thymoquinone. Taken together, the results indicate that thymoquinone exerts anti-metastatic activity on pancreatic cancer both in vitro and in vivo, which may be related to down-regulation of NF-kappaB and its regulated molecules such as MMP-9 protein. Consequently, these results provide important insights into thymoquinone as an antimetastatic agent for the treatment of human pancreatic cancer.

Objective To explore the clinical effects of aminophylline on bronchiolitis in children.Methods One hundred and twen-ty-eight children with bronchiolitis in our hospital were randomly divided into the experiment and treated control group,in the meantime,and divided into the severe and mild group according to their condition.The children in control group were treated with traditional methods,in experiment group were treated with aminophylline(2 mg/kg,every day one time),as well as used traditional methods.All patients in 2 groups were checked by volume (VT), inhale time and exhale time ratio (Ti/tE),time to peak expiratory flow and expiratory time ratio(tPTEF/Te),time to peak expiratory flow to total expiratory time and volume ratio(vPTEF/vE) respectively. Meanwhile pulmonary function,alkyla-denine glycosylase in trachea secretion,IL-8,cytotaxonomy in trachea secretion on time,after the third day and the 7th day.Their curative effects were evaluated.All parameters were analyzed by statistical methods.Results Cytotaxonomy of the patients in the experiment were more improved than that of control group(P