2.Blastic variant natural killer cell lymphoma: report of a case.
Hong JI ; Gan-di LI ; Wei-ping LIU ; Wen-yan ZHANG ; Feng-yuan LI ; Juan LI ; Wei JIANG
Chinese Journal of Pathology 2007;36(1):64-66
CD56 Antigen
;
metabolism
;
Diagnosis, Differential
;
Female
;
Humans
;
Immunohistochemistry
;
Killer Cells, Natural
;
metabolism
;
pathology
;
Leukocyte Common Antigens
;
metabolism
;
Leukosialin
;
metabolism
;
Lymphoma, Extranodal NK-T-Cell
;
metabolism
;
pathology
;
Lymphoma, Non-Hodgkin
;
metabolism
;
pathology
;
Middle Aged
;
Skin Neoplasms
;
metabolism
;
pathology
3.The study on techniques for monitoring intraocular pressure.
Jiang-hong GAN ; Li-ping ZHOU ; Long XU
Chinese Journal of Medical Instrumentation 2009;33(3):202-206
This paper aims at providing an up-to-date review of principle and application of various tonometers, and analyzes the new techniques of diurnal IOP monitoring and design of sensor system abroad. Requirements and test methods for GB/T 14710-93.
Equipment Design
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Intraocular Pressure
;
Tonometry, Ocular
;
instrumentation
;
methods
4.Effect of noise on the auditory system and the intelligence development of premature infants treated in the neonatal intensive care unit.
Wei-Guang LI ; Hong-Bin JIANG ; Tian GAN ; Wen-Xin ZHOU ; Ming CHEN
Chinese Journal of Contemporary Pediatrics 2009;11(12):976-979
OBJECTIVEHigh noise levels (>70 dB) in the neonatal intensive care unit (NICU) are common in some primary hospitals. This study aimed to investigate the noise in the NICU on auditory system and intelligence development in premature infants.
METHODSOne hundred premature infants with respiratory distress syndrome who needed mechanical ventilation therapy were randomly divided into observation and control groups according to the use of earmuffs. The duration of mechanical ventilation therapy lasted for 2 to 15 days in the two groups. After weaning from mechanical ventilator, the auditory brainstem response, cranial B-ultrasonography, and the intelligence development assessment were performed.
RESULTSThe percentage of total (23% vs 47%) and mild hearing loss (15% vs 35%) in the observation group was significantly lower than that in the control group (p<0.05) 2 to 3 days after weaning from mechanical ventilator. The incidence of periventricular hemorrhage intraventricular hemorrhage (PVH-IVH) or periventricular leukomalacia (PVL) in the observation group was significantly lower than that in the control group (21% vs 42%; p<0.05). The intelligence development assessment performed in the first 6 and 12 months of life showed that the mental development index and the psychomotor development index in the observation group were much higher than those in the control group (p<0.05).
CONCLUSIONSThe noise in the NICU can result in mild hearing loss and retardation of intelligence development and increase the incidence of PVH-IVH and PVL in premature infants. The use of earmuff may reduce the adverse events.
Cerebral Hemorrhage ; epidemiology ; Child Development ; Female ; Hearing ; Humans ; Infant, Newborn ; Infant, Premature ; Intelligence ; Intensive Care Units, Neonatal ; Leukomalacia, Periventricular ; epidemiology ; Male ; Noise ; adverse effects
5.Application of BIOMED-2 primers in analysis of T-cell receptor gamma gene rearrangements in paraffin-embedded tissue specimens of T-cell lymphoma.
Yuan TANG ; Wei JIANG ; Lei LI ; Hong JI ; Yun LI ; Gan-di LI ; Wei-ping LIU
Chinese Journal of Pathology 2009;38(4):253-257
OBJECTIVETo evaluate the practical values of PCR detectable T-cell receptor (TCR) gene rearrangement in paraffin embedded tissue samples in the diagnosis of T-cell malignancies using BIOMED-2 PCR multiplex tubes TCRgamma(A+B).
METHODSTraditional phenol-chloroform method was used to extract DNA from 55 cases of archival paraffin embedded tissues samples of T-cell malignancies and the DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-globin. The selected BIOMED-2 PCR multiplex tubes TCRgamma(A+B) were used to detect TCR gene rearrangement and comparison with the results of universal TCR primers (T(VG)/T(JX)) was performed.
RESULTSPositive detection rates by the BIOMED-2 multiplex tubes TCRgamma(A+B) and the universal primers (T(VG)/T(JX)) were 76.4% and 60.0%, respectively. There were not statistical difference between the methods (P > 0.05).
CONCLUSIONBIOMED-2 multiplex tubes TCRgamma(A+B) is suitable for detection of clonal rearrangements of TCR genes in current archival paraffin embedded tissue samples of T-cell malignancies.
DNA Primers ; DNA, Neoplasm ; analysis ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Humans ; Lymphoma, T-Cell ; genetics ; metabolism ; pathology ; Paraffin Embedding ; Polymerase Chain Reaction ; methods ; beta-Globins ; metabolism
6.Study of ATP7A gene in a Chinese family with Menkes disease
Cui-Lian FENG ; Su-Zhen FANG ; Xi-Zhong ZHOU ; Min GAN ; Jiang DU ; Hong LI
Chinese Journal of Neuromedicine 2012;11(1):42-45
Objective To analyze the A TP7A gene mutations in 2 related Chinese patients with Menkes disease (MD) and other members of the family and their hereditary features. Methods Two patients were clinically diagnosed as having MD. All 23 exons and exon-intron boundaries of ATP7A gene were polymerase chain reaction (PCR)-amplified and directly sequenced for genomic DNA extracted from the peripheral blood of both 2 patients and other members of the family; healthy controls were employed too. The mutations were proved by fluorescence quantitative PCR. Results Gross deletions from exon 2-12 were found in these 2 patients,respectively; their mothers,grandmother and aunt with normal phenotype carried those heterozygous mutations in the same site of A TP7A gene.Conclusions The 2 patients with MD are identified by gene and gross deletions from exon 2-12 are reported.
7.Differential diagnosis of nodular lymphocyte-predominant Hodgkin lymphoma and lymphocyte-rich classic Hodgkin lymphoma: role of immunohistochemistry.
Yan-mei HE ; Gan-di LI ; Feng-yuan LI ; Wei JIANG ; Hong JI ; Dian-ying LIAO ; Wei-ping LIU ; Yong-chun LI ; Wei-feng LI ; Yu CHEN ; Yong-hong YANG ; Sheng-xian WANG ; Zhi-rong YANG
Chinese Journal of Pathology 2007;36(6):416-417
Adolescent
;
Adult
;
Aged
;
Child
;
Diagnosis, Differential
;
Female
;
Herpesvirus 4, Human
;
isolation & purification
;
Hodgkin Disease
;
classification
;
metabolism
;
pathology
;
virology
;
Humans
;
Lymphocytes
;
pathology
;
Male
;
Middle Aged
;
PAX5 Transcription Factor
;
metabolism
;
RNA, Viral
;
metabolism
;
Trans-Activators
;
metabolism
;
Young Adult
8.Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits.
Mao ZHANG ; Yue-feng MA ; Jian-xin GAN ; Guan-yu JIANG ; Shan-xiang XU ; Xiang-luo TAO ; An HONG ; Jiao-kun LI
Journal of Zhejiang University. Science. B 2005;6(7):637-643
The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 microg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 microg/(kg.h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-alpha was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-alpha and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.
Animals
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Brain
;
drug effects
;
pathology
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Brain Ischemia
;
drug therapy
;
pathology
;
Fibroblast Growth Factor 2
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administration & dosage
;
Infusions, Intravenous
;
Rabbits
;
Reperfusion Injury
;
drug therapy
;
pathology
;
Treatment Outcome
9.Atorvastatin use and coronary flow reserve in patients with coronary slow flow.
Ying FAN ; Shu-Sen YANG ; Jiang-Bo YU ; Jin-Hong HAO ; Wei HAN ; Run-Tao GAN ; Zheng WANG ; Wei-Min LI ; Yong-Lin HUANG
Chinese Journal of Cardiology 2010;38(2):143-146
OBJECTIVETo investigate the impact of statin use on coronary flow reserve (CFR) in patients with slow coronary flow.
METHODSA total of 91 patients with chest pain and coronary slow flow but normal coronary angiography were included in this study, patients were divided into statin group (atorvastatin 20 mg/d for 8 weeks, n = 51) and non-statin group (n = 40), 26 healthy subjects with normal angiography and negative exercise ECG test served as normal controls. Blood cholesterol was measured. Doppler coronary flow velocity and Doppler reserve measurement of distal left anterior descending were recorded at rest and adenosine infusion (140 microgxkg(-1)xmin(-1)) induced hyperemia state, CFR was calculated by the ratio of maximal hyperemia and baseline peak diastolic coronary flow velocity (hCFV and bCFV) before and after atorvastatin treatment.
RESULTS(1) Eight weeks later, total cholesterol and LDL-C levels were significantly lower in statin group than in non-statin group and control group [TC (3.83 +/- 0.80) mmol/L vs. (5.30 +/- 1.18) mmol/L vs. (5.32 +/- 1.17) mmol/L, P < 0.05; LDL-C (2.26 +/- 0.64) mmol/L vs. (3.28 +/- 0.85) mmol/L vs. (3.30 +/- 0.82) mmol/L, P < 0.05]. (2)Baseline CFR levels were significantly lower in statin group and non-statin group than that in control group (2.32 +/- 0.30 vs. 2.25 +/- 0.33 vs. 3.15 +/- 0.34, P < 0.05). Compared with non-statin group and statin group before treatment, 8 weeks statin treatment was associated with reduced bCFV [(26.06 +/- 3.22) cm/s vs. (29.02 +/- 3.36) cm/s and (26.06 +/- 3.22) cm/s vs. (28.43 +/- 3.40) cm/s, P < 0.05], increased hCFV [(77.63 +/- 8.96) cm/s vs. (65.17 +/- 7.22) cm/s and (77.63 +/- 8.96) cm/s vs. (64.58 +/- 6.26) cm/s, P < 0.05] and increased CFR (3.07 +/- 0.29 vs. 2.28 +/- 0.35 and 3.07 +/- 0.29 vs. 2.32 +/- 0.30, P < 0.05). bCFV, hCFV and CFR of statin group post treatment were similar to those of controls (P > 0.05).
CONCLUSIONPatients with coronary slow flow were associated with lower CFR which could be significantly improved by statin therapy.
Adult ; Aged ; Anticholesteremic Agents ; therapeutic use ; Atorvastatin Calcium ; Coronary Artery Disease ; drug therapy ; physiopathology ; Female ; Fractional Flow Reserve, Myocardial ; Heptanoic Acids ; therapeutic use ; Humans ; Male ; Middle Aged ; Pyrroles ; therapeutic use
10.Diagnostic value of A103 and inhibin-alpha in adrenocortical tumors: an immunohistochemical study using tissue microarray techniques.
Hong-Ying ZHANG ; Xiao-Jie WANG ; Wei-Ping LIU ; Li-Li JIANG ; Gan-Di LI ; Jia GUO ; Yuan-Heng ZHANG
Chinese Journal of Pathology 2004;33(3):203-207
OBJECTIVETo investigate the potential diagnostic value of A103 and inhibin-alpha in adrenocortical tumors and to evaluate the applicability of tissue microarray/tissue chip in pathological studies using immunohistochemistry.
METHODSA tissue microarray/tissue chip was constructed to contain 179 formalin-fixed, paraffin-embedded adrenal tissue samples which include 3 normal adrenal cortex, 2 fetal adrenal cortex, 2 nodular adrenocortical hyperplasia samples, 72 adrenocortical adenomas, 39 adrenocortical carcinomas, 3 adrenal medulla, 13 metastatic carcinomas, 4 metastatic malignant melanomas and 44 pheochromocytomas. Additional 20 cases of normal adult adrenal gland were used as controls. Immunohistochemical markers, including A103, inhibin-alpha, calretinin and Ki-67 were used on the tissue array sections by EnVision immunohistochemical staining methods.
RESULTSPositive staining of A103 was seen in all of the 23 (100%) adrenal cortex, 2 fetal adrenal cortex, 2 nodular adrenocortical hyperplasia samples, 60 of 66 (90.9%) adrenocortical adenomas samples, 35 of 37 (94.6%) adrenocortical carcinomas samples, 3 of 3 malignant melanomas, but in none of the adrenal medulla, pheochromocytomas or adrenal metastatic carcinoma samples. In all of the adrenal cortex, fetal adrenal cortex and nodular adrenocortical hyperplasia cases, inhibin-alpha immunoreactivity was limited to the zona reticularis and the innermost zona fasciculata. Fifty of the 66 (75.8%) adrenocortical adenomas, 28 of the 37 (75.7%) adrenocortical carcinomas were positive for inhibin-alpha. None of the adrenal medulla, pheochromocytoma, metastatic malignant melanoma or carcinoma samples showed a positive inhibin-alpha immunostain.
CONCLUSIONSThe tissue microarray/tissue chip technique provides a reliable method to investigate marker expression by offering a rapid, economic and accurate screening of tissue specimens on a large scale. The combined use of A103 and inhibin-alpha is valuable in distinguishing adrenocortical tumor from pheochromocytoma and other metastatic neoplasms.
Adrenal Cortex ; metabolism ; Adrenal Cortex Neoplasms ; diagnosis ; metabolism ; secondary ; Adrenocortical Adenoma ; diagnosis ; metabolism ; Adult ; Antigens, Neoplasm ; biosynthesis ; genetics ; Female ; Humans ; Immunohistochemistry ; Inhibins ; biosynthesis ; genetics ; MART-1 Antigen ; Male ; Neoplasm Proteins ; biosynthesis ; genetics ; Oligonucleotide Array Sequence Analysis ; Pheochromocytoma ; diagnosis ; metabolism