Objective To observe the therapeutic effects of medicated thread moxibustion of traditional Zhuang nationality medicine combined with Shugan Jianpi Decoction for cirrhosis ascites. Methods Sixty patients with cirrhosis ascites were randomly divided into observation group and control group by random number method, 30 cases in each group. The treatment group received medicated thread moxibustion of traditional Zhuang nationality medicine combined with Shugan Jianpi Decoction and regular Western medicine; while control group only received regular Western medicine. Two weeks was a course of treatment. The liver function (ALT, AST, TBIL, ALB, A/G), ascites integral changes and improving time of main clinical symptoms (abdominal distension, pain, anorexia, fatigue) were observed. The clinical curative effects of two groups were evaluated with 3 months of follow-up. Results The liver function (ALT, AST, TBIL, ALB, A/G) in the treatment group after treatment was significantly improved, and the ascites integral decreased, which was better than the control group, with statistical significance (P<0.05). The improving time of main clinical symptoms in the treatment group was significantly shorter than the control group (P<0.05);The clinical effective rate was 90.00%(27/30) in the treatment group and 76.67% (23/30) in the control group, with statistical significance (P<0.05). Conclusion Medicated thread moxibustion of traditional Zhuang nationality medicine combined with Shugan Jianpi Decoction has good efficacy for cirrhosis ascites.

Objective To explore nursing logo applying in urology safety management,and ensure clinical medical nursing and patients' safety,secure the safety of nursing work orderly.Methods 120 patients hospitalized in July,2010 were tandomly divided into experimental and control group with 60 cases in each group.Both groups accept general behavior instruction with experimental group applying nursing logo in addition.Patients were surveyed about the satisfaction of nursing logo and nursing safety measures at discharge respectively.Results The satisfaction rate were 96.7％ and 58.4％ in experimental and control group respectively,the difference was statistically significant( x2 =20.15,P ＜0.01 ).Conclusions Nursing logo can effectively prevent the occurrence of recessive unsafe factors,can improve patients' satisfaction and security.

Objective To compare the differences between conventional Mammotome biopsy of benign breast tumor and Mammotome biopsy under the electronic clinical pathway,in order to provide basis for Mammotome biopsy of benign breast tumor under clinical pathway.Methods 160 patients with benign breast tumor in affiliated Hospital of Binzhou Medical College from January 2010 to December 2011 were divided into the control group (80 patients treated with conventional Mammotome biopsy) and the observational group (80 patients treated with Mammotome biopsy under clinical pathway).Patients' waiting days before surgery,days in hospital,total cost,obedience,health knowledge,postoperative complications and satisfaction between two groups were compared.Results Days before surgery in the observational group were (1.25 ± 0.33),and days in hospital were (4.28 ± 1.52),both of which were shorter than those in the control group (2.44 ± 0.65),(6.02 ± 4.12).The differences were statistically significant (t =7.48,9.86,respectively ; P ＜ 0.05).Total cost in the observational group was (4 758.48 ± 686.34) yuan,fewer than that in the control group which was (4 998.76 ± 778.32) yuan,and the differences were statistically significant (t =3.46,P ＜ 0.05).Compared with the control group,the observational group had better obedience rate (97.5％ vs 85％),better mastering of health knowledge (97.5％ vs 82.5％),and higher satisfaction (97.5％ vs 85％).The differences were statistically significant (x2 =7.83,10.0,7.83,respectively ; P ＜ 0.05).Conclusions Mammotome biopsy under clinical pathway can further improve patients' obedience and satisfaction,reduce days in hospital,total cost and postoperative complications,thus should be promoted actively.

creatinine concentration. Conclusion Bisphosphonates can decrease serum total calcium levels in hypercalcemia crisis caused by PHPT effectivelywith mild adverse events.

The objective of this study was to detect the expression frequency of HLA-DR15 in patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS), to investigate the relation of expression frequency with diseases and to analyze the relationship between immunoglobulin, T lymphocyte subsets and HLA-DR15. HLA-DR15 expression was detected by PCR-SSP; immunoglobulin was detected by immune turbidimetry; T cell subsets were detected by flow cytometry. The results showed that the expression rates of HLA-DR15 in AA and MDS as well as normal control groups were 78.6%, 63.2% and 24.6% respectively. The difference between AA, MDS and the normal control groups was statistically significant (p < 0.01). OR (odds ratios) values of AA and MDS groups were 11.262, 4.710 respectively. Compared with normal control group, expression rate of HLA-DR15 in hematologic malignancy group was not significantly different. The immunoglobulin level and abnormal T cell subsets in AA and MDS groups were statistically different between HLA-DR15 positive and negative groups (p > 0.05). It is concluded that the frequency of HLA-DR15 antigen in AA and MDS patients is significantly higher than that in normal control and hematologic malignancy group. OR value>1 showed a positive correlation between the diseases and HLA-DR15. HLA-DR15 is a susceptible gene in AA and MDS. The abnormalities of immunoglobulin level and ratios of T cell subsets in AA and MDS are common, but are not associated significantly with the expression of HLA-DR15.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anemia, Aplastic ; immunology ; metabolism ; Case-Control Studies ; Female ; Flow Cytometry ; HLA-DR Antigens ; immunology ; metabolism ; HLA-DR Serological Subtypes ; Histocompatibility Testing ; Humans ; Immunoglobulins ; immunology ; metabolism ; Male ; Middle Aged ; Myelodysplastic Syndromes ; immunology ; metabolism ; T-Lymphocyte Subsets ; immunology ; metabolism ; Young Adult

OBJECTIVEThe cascade of physiological events underlying hypoxic-ischemic brain damage (HIBD) remains to be fully established. The perinatal brain shows both an increased tolerance to hypoxic-ischemic (HI) injury and a faster and more complete recovery than the adult. It is, therefore, important to understand the sequence of events following hypoxia and ischemia in young animals. The present study aimed to clarify the time-course of the activation of the mu-calpain, and the expression of c-Fos, c-Jun, HSP70 and HSP27 proteins following severe HI (2 h hypoxia) and their relationship with each other.

METHODSA modified newborn rat model of HIBD that included a combination of hypoxia and ischemia as described by Rice was used. Forty-two postnatal 7-day-old Sprague-Dawley rats were randomly divided into seven groups (6 rats in each): 6 time-window groups and a normal control group. Samples were collected at 0, 1, 2, 4, 12 and 24 h after HI insults. The protein concentration was determined using a modified Bradford assay. mu-calpain activation, c-Fos, c-Jun, HSP70 and HSP27 expressions were observed respectively by Western blot from cortical and hippocampal samples.

RESULTSThe cleavage of cytosolic mu-calpain was observed from both cortical and hippocampal samples in neonatal rats after HI. The ratio 76:80 of mu-calpain was increased significantly post-HI and reached a maximum at 24 h in cortex and at 12 h in hippocampus after HI. The expressions of c-Fos and c-Jun from both cortical and hippocampal samples in neonatal rats were up-regulated and peaked at 2 or 4 h after HI, demonstrating significant differences at 1, 2, 4, and 12 h compared with that observed in the control (P < 0.05). When compared with that observed in cortex, the nuclear c-Fos expression from hippocampal samples was highly elevated at 2, 4 and 12 h but significantly decreased at 24 h after HI (P < 0.05), while the nuclear c-Jun expression from hippocampal samples was highly elevated at 0 and 1 h but significantly decreased at 4 and 24 h after HI (P < 0.05). Similarly, the expressions of HSP70 and HSP27 from both cortical and hippocampal samples were up-regulated and reached a maximum at 12 or 24 h after HI, demonstrating significant differences at 12 or 24 h both in cortex and hippocampus for HSP70, and at 24 h in cerebral cortex as well as at 12 and 24 h in hippocampus for HSP27 compared with the control (P < 0.05). Furthermore, in comparison with that observed in cortex, the HSP70 expression from hippocampal samples was highly elevated at 1 h, but significantly decreased at 4, 12 and 24 h after HI (P < 0.05), while the HSP27 expression was permanently elevated in hippocampus after HI.

CONCLUSIONThe neuronal injury induced by HI insults appears to involve many ongoing and simultaneous mechanisms. HI activates the calpains immediately, which may contribute to neuron apoptosis, and induces a significant brain neuroprotection, since there is an increased HSP70 expression and a relatively late remarkable HSP27 expression in hypoxic-ischemic neonatal rat brain. Nuclear c-Fos and c-Jun may participate in the pathogenesis of HIBD.

Animals ; Animals, Newborn ; Blotting, Western ; Brain ; metabolism ; pathology ; Calpain ; metabolism ; Enzyme Activation ; Female ; HSP27 Heat-Shock Proteins ; HSP70 Heat-Shock Proteins ; metabolism ; Heat-Shock Proteins ; metabolism ; Hypoxia, Brain ; metabolism ; Male ; Neoplasm Proteins ; metabolism ; Proteins ; metabolism ; Proto-Oncogene Proteins c-fos ; metabolism ; Proto-Oncogene Proteins c-jun ; metabolism ; Rats ; Rats, Sprague-Dawley ; Time Factors

OBJECTIVETo investigate whether activation of mitochondrial aldehyde dehydrogenase 2 (ALDH2) and inhibition of mitochondrial permeability transition pore (mitoPTP) were involved in the cardioprotection of ethanol postconditioning in isolated rat heart.

METHODSHearts isolated from male Sprague-Dawley rats were perfused on a langendorff apparatus and subjected to 30 min of regional ischemia (occlusion of left anterior descending artery) followed by 120 min of reperfusion. The ventricular hemodynamic parameters and lactate dehydrogenase (LDH) release during reperfusion were measured. Infarct size was measured by TTC staining method and the expression of ALDH2 at mRNA level of left anterior myocardium was detected by RT-PCR.

RESULTIn contrast to ischemia and reperfusion, ethanol postconditioning improved the recovery of left ventricular developed pressure, maximal rise/fall rate of left ventricular pressure during reperfusion, reduced LDH release and infarct size. The expression of ALDH2 mRNA level was increased. Administration of mitoPTP activator atractyloside attenuated the effect of ethanol postconditioning, LDH release and infarct size were increased, and the recovery of hemodynamic parameters was inhibited. The expression of ALDH2 mRNA was decreased.

CONCLUSIONEthanol postconditioning has cardioprotection effect, which may be associated with upregulating mitochondrial ALDH2 mRNA expression and inhibiting the opening of mitochondrial permeability transition pore.

Aldehyde Dehydrogenase ; drug effects ; genetics ; metabolism ; Aldehyde Dehydrogenase, Mitochondrial ; Animals ; Ethanol ; pharmacology ; In Vitro Techniques ; Ischemic Postconditioning ; L-Lactate Dehydrogenase ; metabolism ; Male ; Mitochondria, Heart ; drug effects ; metabolism ; Mitochondrial Membrane Transport Proteins ; drug effects ; metabolism ; Mitochondrial Proteins ; drug effects ; genetics ; metabolism ; Myocardial Reperfusion Injury ; metabolism ; pathology ; prevention & control ; Myocardium ; metabolism ; pathology ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

OBJECTIVERespiratory syncystial virus (RSV) is the most common cause of lower respiratory infections in infants worldwide. There is no reliable vaccine or antiviral drug against RSV at present. RNA interference (RNAi) technology is a potent method to degrade expression of the cognate mRNA. In order to inhibit the replication of RSV at gene level, the effects of specific RNAi against M2-1 gene of RSV on inhibition of viral replication in cell culture system was observed in this study.

METHODSRSV M2-1 gene, which plays a key role in RSV transcription, was chosen in this study and was used as target gene and recombinant plasmid pshRNA7816 targeting the mRNA of RSV M2-1 gene coding sequence was constructed. The pshRNA7816 was transfected into Hep2 cells. The effects of the pshRNA7816 on changes of cytopathogenic effect (CPE) of Hep2 cell induced by RSV infection were observed microscopically. Viral plaque forming assay and MTT assay were used to detect the viral titer change and protective function of the pshRNA7816 on RSV infected Hep2 cell.

RESULTSThe recombinant RNAi plasmid pshRNA7816 which targets the mRNA of RSV M2-1 gene was successfully constructed. The pshRNA7816 significantly reduced CPE of RSV infected Hep2 cells, reduced the viral titer of RSV in the cells (P < 0.001). The pshRNA7816 raised the survival rate of RSV infected Hep2 cells (P < 0.001). Non-specific pshRNA plasmid did not show anti-RSV effects (P > 0.05).

CONCLUSIONThe recombinant pshRNA7816 plasmid which targeted the mRNA of RSV M2-1 gene showed a significant and specific anti-RSV effect.

Hep G2 Cells ; Humans ; Plasmids ; biosynthesis ; RNA Interference ; RNA, Small Interfering ; biosynthesis ; RNA, Viral ; genetics ; Respiratory Syncytial Virus Vaccines ; biosynthesis ; Respiratory Syncytial Virus, Human ; drug effects ; physiology ; Viral Proteins ; genetics ; Virus Replication ; drug effects

OBJECTIVETo Verify the safety and reliability of one-stage repair of complete cleft Lip and palate in infancy and to obtain the primary result.

METHODSThe simultaneous repair of complete cleft Lip and palate in infants 3 to 12 months of age were performed in 271 cases. The deformities include 185 cases of typical complete unilateral clefts and 75 cases of complete bilateral clefts, and other 11 atypical cleft infants. The preoperative orthopedic treatment for wide alveolar cleft was undertaken in 24 infants and the lip appearance and speech outcome were evaluated in 116 children by 1 to 4 years' postoperative follow-up.

RESULTSAll infants, except for dyspnea in 2 babies, palatal fistula formation in 6 cases and temporary wound hemorrhage in 5 infants, were recovered without complications. After orthopedic treatment, the width of the alveolar cleft was reduced 6.1 mm in average. The evaluation showed that 93.1% of children had got good or excellent lip appearance. And the acceptable or excellent speech was found in 94.8% children.

CONCLUSIONSSimultaneous repair of complete cleft lip and palate in infancy is safety and reliable. The preoperative orthopedic procedure is able to reduce the wide alveolar cleft and to achieve alignment of alveolar segments. The acceptable and or excellent lip appearance and speech function could be obtained in this one-stage operative procedure in infants.

Cleft Lip ; surgery ; Cleft Palate ; surgery ; Female ; Humans ; Infant ; Male ; Treatment Outcome

Chemical constituents of ethyl acetate extract of Artemisia rupestris were isolated and purified by various chromatographic methods, including silica gel, ODS and Sephadex LH-20. Their structures were elucidated on the basis of spectroscopic data analysis. 12 compounds were separated from A. rupestris and their structures were identified as 5,4'-dihydroxy -3,6,7-trimethoxy flavone (1), R-(-) -vestitol (2), tricin (3), chrysoeriol (4), 3-indole carboxylic acid (5), esculetin (6), apigenin (7), luteolin (8), trans-caffeic acid (9), casticin (10), chrysosptertin B (11) and artemetin (12). Compound 2 was obtained from the genus Artemisia for the first time, and compounds 1-6 and 9 were separated from this plant for the first time.
Artemisia ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Mass Spectrometry ; Molecular Structure