1.Preliminary experience in the treatment of chronic voiding dysfunction by sacral nerve neuromodulation (report of 2 cases)
Keji XIE ; Shaojun JIANG ; Ping TANG
Chinese Journal of Urology 2001;0(09):-
50%) in the objective findings and/or subjective symptoms in both patients.In case 1 and case 2,the frequency of daily urination decreased by 7.3% and 34.3%,the volume of urination increased by 118.2% and 65.7%,the degree of urgent urination declined by 72.0% and 68.1%,respectively.Then they received permanent electrode and neurostimulator implantation and the improvement remained significant.They were followed up for 26 and 17 months,respectively,and no significant complications were found. Conclusions SNN is a micro-invasive,effective,and safe therapy for chronic voiding dysfunction.It may also be effective for some neurogenic dysfunctions of the bladder and urethra.
2.Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro
Tong XIE ; Ping WU ; Deshen TANG ; Limin JIANG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS: GMC proliferation was detected by MTT method, GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis. The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS: The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION: CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.
3.Effects of cholinergic receptor antagonists, adrenergic drugs and pethidine on the function of sphincter of Oddi isolated from rabbits
Ping CHEN ; Dandan LI ; Congxun JIANG ; Chengwei TANG
Chinese Journal of Digestion 2010;30(9):593-596
Objective To investigate the contractive effect of atropine, noradrenaline,adrenaline and pethidine on sphincter of Oddi (SO) isolated from rabbits. Methods The rings of SO isolated from 60 rabbits were treated with Krebs solution and then were exposed to gradient atropine,anisodamin, noradrenalin, adrenaline or pethidine with 10 each. The rest 10 rings of SO treated with Krebs solution only were served as controls. The amplitude and frequency of contraction of SO were recorded. Results Compared with control group, all of the 5 medicines were able to significantly decrease the contractive amplitude, but not frequency, of SO at the concentrations ranged from 10-6 mol/L to 10-2 mol/L (P<0.05). The inhibition order was as follows: noradrenaline > adrenaline >atropine > anisodamin ≈ pethidine. Conclusions Beside atropine and anisodamin, noradrenaline,adrenaline and pethidine also showed the direct relaxation of SO by decreasing contractive amplitude of SO. Adrenaline or noradrenaline displayed stronger effect in decreasing contractive amplitude of SO when compared with atropine or anisodamin.
4.Clinical analysis and intervention strategies in 67 cases of pregnancy complicated with heart failure
Yong WANG ; Chun YANG ; Ping TANG ; Jing JIANG
Chinese Journal of General Practitioners 2012;11(5):375-376
To explore the clinical characteristic and intervention strategies for pregnancy complicated with heart failure. The clinical data of 67 pregnant women with heart failure during January 1998 to December 2010 were collected and analyzed.The rate of heart failure in pregnancy with heart diseases was 31% (67/216).The causes of pregnancy complicated with heart failure were peripartum cardiomyopathy ( n =33,49% ) and hypertensive disorder complicating with pregnancy heart disease( n =28,42% ).Heart failure in pregnancy appeared at an average of (36.0 ± 2.7 ) weeks.The average terminating time of pregnant women with heart failure was ( 36.5 ± 2.1 ) weeks.Cesarean section was the main safe choice of delivery for pregnant women with heart failure.The average weight of newborns for pregnant women with heart failure was(2517 ±541 )g.The rate of neonatal asphyxia was 22% (11/50).Strengthening routine antenatal examination and treating pregnant complications timely can prevent and reduce the incidence of heart failure.
5.Expression of CD143 and its significance in focal nodular hyperplasia of liver.
Lei SHI ; Li-li JIANG ; Wei-ping LIU ; Yuan TANG
Chinese Journal of Pathology 2006;35(7):421-422
Adenoma, Liver Cell
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metabolism
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pathology
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Adolescent
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Adult
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Child
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Diagnosis, Differential
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Female
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Focal Nodular Hyperplasia
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metabolism
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pathology
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Humans
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Immunohistochemistry
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Liver
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chemistry
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pathology
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Liver Neoplasms
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metabolism
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pathology
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Male
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Middle Aged
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Peptidyl-Dipeptidase A
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biosynthesis
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Young Adult
6.Expression of CDK4 and cyclin D1 proteins in gastric carcinoma
juan, LI ; ping-ping, WU ; xu-cheng, JIANG ; zhao-wen, YAN ; jian-min, TANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To investigate the expression and significance of CDK4 and cyclin D1 in gastric carcinoma,and to discuss their correlation with WHO histological classification,TNM stages and lymph node metastasis.Methods Forty paraffin wax specimens from patients with gastric carcinoma were collected and expression of CDK4 and cyclin D1 proteins were detected by immunohistochemistry.Results Immunohistochemistry demonstrated that the positive rates for CDK4 and cyclin D1 protein were 65% and 55%,respectively.There was positive correlation between CDK4 and cyclin D1 proteins(P0.05).Conclusion CDK4 and cyclin D1 proteins are frequently overexpressed in gastric carcinoma and there is a positive relationship between the two proteins,which may be involved in the development of gastric carcinoma.
7.Research status of natural compounds combine with antifungal agents against drug-resistent Candida albicans.
Jiang-Fan WAN ; Chun-Ping TANG ; Zhi-Bin SHEN ; Tao JIANG
China Journal of Chinese Materia Medica 2014;39(1):28-33
To against the emergence of drug-resistent candidiasis, the studys of synergism of natural compounds combine with antifungal agents in vitro showed a continuous growth in recent years. The paper reviewed recent progresses to compare the synergetic effect by FICI method, and to conclude the synergetic mechanisms which have been confirmed as a reference for futher study.
Antifungal Agents
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therapeutic use
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Candida albicans
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drug effects
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Drug Combinations
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Drug Resistance, Microbial
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drug effects
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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methods
9.A Study on HPLC Fingerprints of Zingiber Officinale Before and After Sulfur-fumigation
Ping LI ; Jun JIANG ; Jian TANG ; Huajun WANG ; Liya GU ; Haotian LI ; Xiaobin JIA
Chinese Journal of Information on Traditional Chinese Medicine 2014;(12):85-87
Objective To explain the differences between zingiber officinale and its sulfur fumigation products on chromatography fingerprints by HPLC-DAD;To discuss the influence of sulfur-fumigation on the quality of zingiber officinale. Methods HPLC, diode array detector, and ZORBAX SB-C18 column were used with acetonitrile-water as the mobile phase, gradient elute, volume flow rate of 1 mL/min, column temperature of 25 ℃, and detection wavelength of 280 nm. HPLC-DAD technology was applied to establish the fingerprints of zingiber officinale before and after sulfur-fumigating process, in order to analyze the HPLC fingerprints of zingiber officinale before and after sulfur-fumigating process. External standard method was used to do the quantitative determination of 6-gingerol. Results The 17 common peaks were identified through the comparison of 3 batches of fingerprints of zingiber officinale and their sulfur-fumigated samples. The peak areas of NO.3, NO.10, NO.11, and NO.17 were reduced by 50.68%, 64.41%, 67.68%, and 21.23%respectively. The content of 6-gingerol had no significant change. Conclusion The chemical composition of zingiber officinale changed at different degrees after sulfur-fumigated process. The safety and effectiveness of sulfur fumigation products of zingiber officinale require more researches.
10.The evaluation of cerebral function by diffusion weighted imaging after mild hypothermia therapy in a procine model of cardiac arrest
Ziren TANG ; Chunsheng LI ; Peng PENG ; Tao JIANG ; Hong ZHAO ; Ping GONG ; Rong HUA
Chinese Journal of Emergency Medicine 2014;(6):630-634
Objective To evaluate the effects of mild hypothermia therapy on cerebral function and edema as assessed by magnetic resonance imaging (MRI ) during the first 72 hours after restoration of spontaneous circulation (ROSC)in a porcine model.Methods Ventricular fibrillation was induced in 33 Inbred Wuzhishan miniature swine.After ventricular fibrillation untreated for 8 min,standard CPR was initiated and 30 animals got ROSC.The ROSC animals were randomly (random number)assigned to normothermia group (n=15 ) and hypothermia group (n =15 ).The hypothermia group immediately received endovascular cooling to regulate temperature to 33 ℃,which was maintained for 12 h,followed by passive rewarming at 0.5 ℃/h to 37 ℃.The two groups were scanned to get Diffusion-weighted imaging (DWI)at baseline (before experiment),6 h,12 h,24 h,and 72 h after ROSC.The statistical analysis was carried out with Student t test,One-way repeated-measures and Kaplan-Meier survival analysis.Results Compared with the normothermia group,the hypothermia group exhibited a higher 72-h survival rate (73.3% vs.40%,P<0.05),lower neurological deficit scores (43.81 vs.119.50;P<0.01),a lesser decrease in the apparent diffusion coefficient during DWI and milder cerebral edema on DWI image. Conclusions Cerebral edema occurred at early stage after ROSC.Cerebral edema was worst at 6 hours after ROSC and then subsided gradually.Hypothermia therapy could reduce cerebral edema and produce a protective effect on neurological function.MRI could effectively reflect the underlying trend of cerebral injury of swine within the first 72 h after ROSC,and demonstrated that hypothermia could reduce brain edema, increase cerebral blood flow,and reduce the production of toxic metabolites.DWI could monitor brain injury after resuscitation and could be used to evaluate the efficacy of hypothermia intervention dynamically.