AIM: To establish the co-culture mode of tissue-engineered tracheal seeding cells, and compare with the routine culture, so as to provide the fundament of tissue-engineered trachea construction by cell compound materials. METHODS: The experiment was completed at the laboratory, Department of Respiratory Diseases, Tangdu Hospital, the Fourth Military Medical University of Chinese PLA from May 2006 to May 2007.①Three healthy New Zealand male rabbits, weighed (250.00?0.75) g, were used in this study.②Co-culture mode: The tracheal epithelial cells and fibroblasts were isolated and then co-cultured for 7-10 days. The two kinds of cells were distinguished according to their different tolerances to trypsin. Subsequently, A hole received 0.5 g/L trypsinization, cells were suspended in DMEM medium containing 0.05 volume fraction of CO2 and then transplanted into B hole (fibroblasts); again, A hole was added with 2.5 g/L trypsin for digestion, afterwards cells were suspended with K-FSM medium and transplanted into C hole (tracheal epithelial cells). Routine culture: after isolation, tracheal epithelial cells were cultured and fibroblasts were purified, respectively.③The co-cultured cells and the traditionally cultured cells were compared via cell growth curve and cell proliferation detected by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. RESULTS: ①Cell growth: Co-cultured cells grew well, those in A hole increased intercellular apace after trypsinization, while cells in B hole were shaped as fusiform, without the manifestation of typical road stone-like cells, and cells in C hole were apposite, showing lamellar road stone-like morphology.②Cell growth curve: The growth curve of co-cultured cells was identical with that of cells by routine culture method.③Cell proliferation: MTT detection revealed no significant difference in the proliferation of co-cultured cells and cells cultured by traditional method (P

This paper aims at analyzing the reform strategies in the clinical teaching for stomatology undergraduates: strengthening the management of clinical education,the establishment of a capable and effective teaching force,stimulating students’ interest in learning,guide their independent learning,the introduction of case teaching method etc,to promote clinical teaching and improve the teaching quality.

Apoptosis is an organized suicide program which is evolutionally conserved from yeast to mammals. Research on yeast apoptosis has made rapid progress, though it remained unrecognized until recent years. Initial observations show that yeast can be induced to undergo apoptosis and a number of conserved pro- and antiapoptotic proteins have been identified in Saccharomyces cerevisiae. Yeast has been validated as a model organism to investigate mechanisms of apoptosis. Recently, yeast has also been used as a model to study apoptosis-related disease, such as Huntington's disease and Parkinson′s disease. The feasibility, the advantages and the perspectives of yeast model for apoptosis research are reviewed.

Objective:To evaluate the role of esophagogastric junction contractile index (EGJ-CI) in distinguishing patients with refractory gastroesophageal reflux disease (RGERD) from functional heartburn (FH).Methods:From March 2014 to January 2018, 82 patients with proton pump inhibitor (PPI) refractory heartburn and/or regurgitation, who visited the Outpatient Department of Gastroenterology at The First Affiliated Hospital with Nanjing Medical University were enrolled, among them 50 patients with RGERD (RGERD group) and 32 patients with FH (FH group). EGJ-CI of RGERD group and FH group were compared. The sensitivity and specificity of EGJ-CI to distinguish RGERD from FH patients. The correlation between EGJ-CI and high resolution esophageal manometry parameters, baseline impedance level and 24 h impedance-pH monitoring parameters were analyzed. Mann-Whitney U test, receiver operator characteristic curve analysis and Spearman correlation analysis were used for statistical analysis. Results:The EGJ-CI of RGERD group was lower than that of FH group (25.8 mmHg·cm (14.1 mmHg·cm, 35.9 mmHg·cm)(1 mmHg=0.133 kPa) vs. 39.2 mmHg·cm (23.0 mmHg·cm, 60.8 mmHg·cm)), and the difference was statistically significant ( Z=-2.833, P=0.005). When the cut-off value of EGJ-CI was 35.8 mmHg·cm, the sensitivity and specificity to distinguish RGERD from FH were 76.0% and 62.5%, respectively; area under the curve was 0.69 (95% CI 0.57 to 0.81). EGJ-CI was positively correlated with lower sphincter resting pressure, integrated relaxation pressure, distal contractile integral, distal esophageal pressure, and mean nocturnal baseline impedance ( r=0.812, 0.631, 0.451, 0.490 and 0.401, all P<0.01). EGJ-CI was negatively correlated with DeMeester score, acid exposure time, total reflux episodes, acid reflux episodes, long reflux episodes and longest reflux time ( r=-0.363, -0.372, -0.346, -0.318, -0.300 and -0.291, all P<0.01). Conclusions:EGJ-CI can help to distinguish patients with FH from RGERD.

Female ; Humans ; Hyperkeratosis, Epidermolytic ; pathology ; Infant, Newborn

Objective To study the positive incidence and clinical significance of auto-antibody of liver antigens in the serum of patients with autoimmune hepatitis diseases and viral hepatitis.Methods The serum samples with hepatic diseases were col-lected in Jiangyin People’s Hospital from 2010 to 2014 year.Patients were divided into three groups according to diseases:autoimmune hepatic disease group including autoimmune hepatitis (AIH)12 cases and primary biliary cirrhosis (PBC)36 case;viral hepatic group including HAV 23 cases,HBV 30 cases,HCV 14 cases and HEV 8 cases;normal control group 30 cases.Auto-antibody of live antigens AMA-M2,LKM-1,LC-1,SLA/LP,GP210 and SP100 were tested respectively by west-ern blotting assay.Results The positive rates of anti-AMA-M2,anti-LKM-1,anti-LC-1,anti-SLA/LP,anti-GP210,anti-SP100 were 16.7%,16.7%,8.3%,25%,0% and 16% respectively in AIH group,83.3%,0%,0%,0%,44.4% and 27.8% respectively in PBC group.Patients with viral hepatitis,one case were anti-AMA-M2 positive,one case were anti-GP210 positive and three case with positive anti-SP100.anti-AMA-M2,anti-GP210 and anti-SP100 were detected more fre-quently in autoimmune liver diseases group than viral hepatitis group (χ2 = 33.9,10.6 and 8.8,P < 0.05),anti-SLA/LP were detected more frequently in AIH group than PBC group (χ2 = 6.4,P <0.05),while anti-AMA-M2,anti-GP210 and were detected more frequently in PBC group than AIH group (χ2 =15.1 and 6.1,P <0.05).The positive rate of viral hepat-ic group was a very low frequency,there are little statistically significant difference (χ2 =1.1,P >0.05)compared with nor-mal control group.Conclusion The antibody of liver antigens are useful clinically for diagnosing and classifying autoimmune liver disease.

Objective To evaluate the diagnostic value of enlargement of the anterior space of the right branch of portal vein in early cirrhosis.Methods 37 cases with early cirrhosis and 42 cases with type B hepatitis proved by pathology as well as 40 health cases underwent MDCT examinations.The width of the anterior space of right branch of portal vein(RBPV)and the diameters of RBPV in each group were measured on CT imaging,then the results were analysed comparatively.Results The width of anterior space of RBPV and the diameters of RBPV were(5.5±2.4)mm and(11.84±1.91)mm in health group,(13.6±5.8)mm and(12.36±61.97)mm in early cirrhosis group,(5.7±3.1)mm and(12.13±1.89)mm in type B hepatitis group,respectively.There were significant differences in the width of the anterior space of RBPV between three groups(P＜0.0001).However,there were not significant differences in the width of RBPV among these three groups(P＞0.05).While,there were significant differences of the width of the anterior space of RBPV and the diameters of the RBPV between normal control and type B hepatitis(P＞0.05).Conclusion CT sign of the anterior space of RBPV increased is of important value in diagnosis of early cirrhosis.

Objective To investigate the effect of ultrasound-guided transversus abdominis plane (TAP)block on the efficacy of postoperative analgesia in parturients undergoing selective cesar-ean delivery.Methods Eighty ASA Ⅰ or Ⅱ parturients recruited for selective cesarean delivery under combined spinal-epidural anesthesia were randomly divided into two groups(n =40 each):TAP group (group T)and control group(group C).After cesarean delivery,bilateral of ultrasound-guided TAP block were performed,20 ml of 0.5% ropivacaine was injected in each side in group T,while TAP was not done in group C.Both groups received patient-controlled intravenous analgesia (PCIA)after cesarean delivery.The resting and exercise visual analogue scale (VAS)scores,Ramsay sedation score and the Bruggrmann comfort scale(BCS)score were evaluated at 2,4,6,8 and 24 h after operation. The consumption of sufentanil within 24 h after operation,the number of successfully delivered doses (D1 )and the number of attempts (D2 )within 24 hr after operation were recorded.D1/D2 was calculated.The parturients satisfaction and the adverse reactions were also recorded.Each parturient was assessed postoperatively by a blinded investigator.Results The consumption of sufentanil within 24 hr after operation,the resting and exercise VAS scores at 2,4,6 hr after surgery were significant-ly lower,while the BCS score,the value of Dl/D2 and the degree of satisfaction were higher in group T than those in group C (P <0.05).There were no adverse reactions in both groups.Conclusion Ultra-sound-guided TAP block reduces the postoperative sufentanil consumption,enhances the efficacy of post-cesarean analgesia of the parturients.Comfort and satisfaction are achieved in the parturients of the group T.

Objective:To construct the expression vectors of VH and VL from an anti -CD71 monoclonal antibody (line7579) , and express an anti-CD71 mouse/human chimeric antibody (D2C) in vitro.Methods:The variable regions of heavy and light chains from an anti -CD71 monoclonal antibody (line7579) cloned in the pGEM-T vectors were constructed respectively into the expression vectors,p?-Expr(including human IgG1 constant region) and p?-Expr(including human Ig? constant region).The expression plasmid vectors (Chi7579-p?-Expr and Chi7579- p?-Expr) ,confirmed by a clone-PCR and a further sequence analysis, were co-transfected by electroporation into the non-Ig-producing murine hybridomas SP2/0.Results:Screening for transfectomas secreting chimeric antibody was done by cross sandwich ELISA, which identified the transfectomas to secrete a chimeric antibody (D2C) with the human heavy constant region(C?) and light constant region(C?). FCM analysis of culture supernatants of the transfectomas demonstrated that the binding capacity of the chimeric Ab (D2C) to the antigen (CD71) was retained.Conclusion:The construction of the V H and V L expression vectors and the expression of an anti-CD71 mouse/human chimeric antibody (D2C) were accomplished successfully.

Objective To evaluate the efficacy and toxicity of navebine(NVB) given by continuous infusion plus cisplatin combination therapy in the treatment of advanced breast cancer.Methods 60 patients with advanced breast cancer were enrolled in this study.The type of his to pathologyis invasive ductal carcinoma.19 patients had no previous chemotherapy but 21 cases received chemotherapy containing ADM.26 patients had ≥2 metasta-ticsites.All the patients received vinorelbine according to the following doses chedule:10mg bolus followed by 10mg by continuous infusion over 24 hours every 5 days every 3 weeks.Results 60 patients were evaluable for response.A total of 131 cycles were administered.The lverall response rate was 41.6%(3 patients achieved a complete response and 22 patients achieved a partial response).The median over all survival was 35 weeks and the median duration of response was 19 weeks.The dose limiting toxicity was marrow-suppression.Neutropenia was in 43% patients(31% was grade Ⅲ and Ⅳ).Thrombo-cytopenia and anemia were mild.Conclusion A highe rsponse is obtained in advanced breast cancer treated by continuous intravenous NVB infusion with tolerable toxicity and deserve further revaluation.