BackgroundJunctional adhesion molecule-1 (JAM-1) is intercellular transmembrane protein newly discovered and associated with the tight junction.Tight junction plays an important role in keeping the transparency of cornea,but there are few studies about JAM-1 in cornea tight junction.ObjectiveThis study was to determine the expression of JAM-1 in corneal epithelium,stroma,endothelium,and locate the distribution of JAM-1.MethodsThe corneas of two SFP Wistar rats were obtained,and the samples of epithelial lamella,corneal stroma and endothelium with Descemet membrane were prepared respectively for the detection of the expression of JAM-1 mRNA using reverse transcription polymerase chain reaction(RT-PCR).Primers were designed according to the genes as RGD web provided,and the objective genes were JAM-1,occludin and claudin-1.Products of PCR were examined by 1.5％ agarose gel electrophoresis and assayed with GelDoc-lt UVP Imaging System.The corneal paraffin sections and stretched preparations of epithelium and endothelium of corneal sections from other two SFP Wistar rats were prepared for the examination of JAM-1protein expression and location by immunohistochemistry.The use of experimental animals followed the Statement of ARVO. Results JAM-1,occludin and claudin-1 mRNA were expressed in rat cornea epithelium,stroma and endothelium.PCR melting curves showed the limpid unimedality.The expression level of JAM-1 mRNA was similar to occludin mRNA and higher than that of claudin-1 with the highest level in the epithelium layer.Immunohistochemistry assay revealed that there was definite JAM-1 antibody staining in the cornea epithelium,stroma and endothelium layers.However,the basal layer of corneal epithelium presented with the strongest staining in comparison with stromal and endothelial layers.Stretched preparations of corneal epithelium and endothelium showed that JAM-1 protein appeared at the junction site of epithelial cells and endothelial cells.The basal layer of the corneal epithelium showed the strongest response,and the staining of corneal endothelium was extensive and diffuse. Conclusions JAM-1 is a composition of intercellular tight junction which expresses in cornea epithelium,endothelium and stroma.However,its appearance and level vary from different corneal layers.

Objective To determine the usefulness of MR diffusion-weighted imaging(DWI) in evaluating obstructive jaundice.Methods 25 patients with obstructed dilating bile ducts but without other basic hepatic diseases and 11 normal controls were studied byusing DWI.25 patients were divided into two groups(14 patients with jaundice and 11 patients without jaundice) depending on the serumlevels of total bilirubin.ADCs of liver were measured at workstation,and the mean ADCs were compared among the groups of patients and controlsusing one-way ANOVA.Results When b=500 s/mm~2,the mean ADCs of liver in three groups were significantly different(F=25.29,P0.05) between the ADCs in patients without jaundice and in controls.When b=300 s/mm~2,the mean ADCs of liver in three groups were significantly different(F=12.22,P

Objective To observe the effect of multiple interventions on secondary epilepsy patients.Methods 114 secondary epilepsy patients were randomly divided into the intervention group and control group with 57 cases in each group.The cases in the intervention group received multiple interventions including heath education,psychological treatment,safety nursing,overdose administration,early rehabilitation,discharge guide and so on besides usual nursing.While,the cases in the control group received usual nursing.The follow-up was performed for 6 months and the therapeutic efficacy of two groups was compared.Results After multiple interventions,the seizure frequency and trauma incidence rate decreased,compliance and quality of life increased in patients of the intervention group,and there was a significant difference compared with the control group(P<0.05).Conclusion Multiple interventions can decrease seizure frequency and increase compliance of treatment and quality of life of secondary epilepsy patients.

Objective To identify nurses' practice of 5A's smoking cessation intervention in clinical settings of Beijing.Methods A cross-sectional survey was conducted among 248 nurses by convenient sampling.The questionnaire included the frequency of nurses' practice of 5A's intervention and its influencing factors,nurses' self evaluation of competency,and tobacco-related curricula in school of nursing.Results Most nurses implemented “advice” and “evaluated the patients' smoking history and current status” more frequently,but nurses' practice of other interventions was dissatisfactory.The belief that smokers could benefit from quitting was facilitating,however the barriers included no motivations to quit smoking,heavy work,and the belief that helping smokers to quit is not the priority in nursing routine.The nurses got insufficient knowledge about smoking cessation through school education and in-service training (12.8％).Conclusions There's a big gap between the implementation of nurses' smoking cessation intervention,accepted smoking cessation education,smoking cessation intervention competency and the urgent demands to help patients quit smoking.

[Objective] The objective of the study was to determine the clinical and radiological outcomes of cervical interbody fusion using ROI cages. [Methods] Patients showing degenerative cervical mono- or bi-level pathology were prospectively included. Using AP and lateral radiographs, segmental height of the treated segments was determined quantitatively. Cage characteristics were described qualitatively. Clinical data such as the neck disability in-dex(NDI), numeric rating scale(NRS) were col ected at al time points .[Results] The scores of NDI, NRS were decreased gradual y, and stable after half year;but they were respectively reduced to 8.64 and 1.57 from 23.32 and 8.93, with difference of statistical meaning. The intervertebral angle increased af-ter operation, then decreased gradual y and was stable after half year, was 3.6 °after 1 year. The intervertebral height was maximum after operation, then re-duced gradual y, and was 5.32mm or so after 1 year. 16 patients were al cured after 1 year, with short fusion rate reaching 100%. [Conclusions] The clini-cal and radiological short-term outcome of anterior cervical discectomy and fusion using ROI cage are excel ent.

Background The impact of statins on inflammation are independent of cholesterol-lowering effect.Recent studies showed that Toll-like receptor 4(TLR4),a mediator of innate immune responses,is involved in the initiation and progression of atherosclerosis.Objective To investigate the effects of atorvastatin on LPS-induced TLR4 expression and downstream signals and to explore the molecular mechanisms of anti-inflammation by statins.Methods Human umbilical vein endothelial cells(HUVEC)were pretreated with atorvastatin(1 or 10 ?mol/L)or NF-?B inhibitor CAPE for 30 min,then incubated by purified LPS for 24 hours.TLR4,ICAM-1 and E-selectin mRNA were measured by RT-PCR;the percentage of TLR4 positive cells were detected by flow cytometry.The activation of NF-?B(p65)were detected by Western blot.Results Atorvastatin(1-10 ?mol/L)prevented LPS-induced increases in TLR4,ICAM-1 and E-selectin expression [TLR4 mRNA(1.24?0.21)vs LPS(1.82?0.27),P

Objective To study the expression and significance of CD44v6 and E-Cadherin( E-cd) in human renal cell carcinoma. Methods Immunohistochemical method was used to detect CD44v6 and E-cd expression in 20 normal renal tissues and 72 primary renal cell carcinoma tissues. Results The postive expression rate of CD44v6 in normal renal tissues and primary renal cell carcinoma tissues was 15 00% and 66 67% respectively with significant difference (P

OBJECTIVE:To evaluate the relative bioavailability of two products(CLMC 1 ,CLMC 2 )of Clarithromycin in man.METHODS:A single oral500mg dose of CLMC 1 or CLMC 2 was given to20healthy male volunteers in an open randomized crossover study.The plasma concentrations of CLMC 1 and CLMC 2 were measured by microbial assay.The pharmacokinetic parameters were calculated with3p97pharmacokinetic program and the bioequivalence was evaluated.RESULTS:The con?centration-time curves of CLMC 1 and CLMC 2 fitted to a two-compartent open model.C max were(2.23?0.83)?g/ml and(2.14?0.70)?g/ml;T max were(1.95?0.39)h,(1.78?0.41)h;AUC (0～T) were(9.50?2.52)(?g?h)/ml,(9.35?2.54)(?g?h)/ml respectively.The relative bioavailability of CLMC 1 was(101.60?9.35)%.CONCLUSION:The results of two and one-side t tests suggest that the CLMC 1 is bioequivalent with the CLMC 2 .

AlM:To explore the suitable conditions in rapid model of corneal neovascularization ( CNV ) after thermal burn under different constant temperature in rabbit.
METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups ( A, B, C, D, E ) . A groups:100℃ ( n = 10 ) , B groups: 200℃ ( n = 10 ) , C groups:300℃ ( n=10 ) , D groups: 400℃ ( n=10 ) , and E groups:control group ( n=5 ) . All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 th , 7 th , 14 th , 30 th days postoperatively. SPSS 19. 0 statistical package was used for data analysis, and the data was recorded by mean ± standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0. 05.
RESULTS:On postoperative 4 th , 7 th , 14 th , 30 th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left (n=2);the area of CNV were (9.16±1.45)mm2, (37.73±5.49)mm2, (62.44± 7. 54 ) mm2 , ( 40. 28 ± 7. 39 ) mm2 in B group respectively;and (11.45±1.04)mm2, (44.51±4.64)mm2, (66.13±4.13)mm2, (43.04±2.33)mm2 in C group respectively; and (13.23± 0.86)mm2,(47.26±4.59)mm2,(67.57±4.56)mm2,(45.59± 4. 44 ) mm2 in D group respectively, and part corneal carbide ( n = 4 ) was observed as well as corneal perforation ( n= 6 ) were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P < 0. 05. Statistical differences were found among B, C, D groups, P<0. 05.
COCLUSlON:ln 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. lt has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. lt is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.

Purpose:To investigate the effects and side- ef fects of thalidomide in the treatment of relapsed and refractory multiple myelom a (MM).Methods:There were thirteen men and two women aged 33～7 6 (median 58) years in thalidomide alone group. Two cases were newly diagnosed my eloma; twelve of them were refractory myeloma, including three relapsed cases wh o had experienced autologous peripheral blood stem cell transplantation (AutoPBS CT); another one is primary plasma cell leukemia. The starting dose was 100mg/d, escalating up to a maximum of 800mg/d. The combination group included twenty me n and seven women aged 40～81 (median 56) years of which case was newly diagnose d myeloma; twenty-five cases were refractory myeloma, two of whom relapsed after AutoPBSCT; another one was primary plasma cell leukemia. Dexamethasone 40mg/d w as used at d 1-4 , d 9-12 , d 17-20 when the dose of thalidomide increased to 400mg/d. Each cycle lasted for one month.Results: The total response rate was 42.9% and 57.7% in thalidomide alone group and in co mbination group, respectively. The response rate in refractory patients was 33.3 % and 44.0% in thalidomide alone group and in combination group, respectively. T here was no difference between the two groups in response rates. Both of the two groups had side effects of constipation and skin rash, etc. in different degree s but usually tolerable. Conclusions:Both thalidomide alone and thalidomide combined with dexamethasone was effective in the treatment of relap sed and refractory MM.