1.Anti-hyperuricemia effect and mechanism of polydatin in mice.
Gao WU ; Han-Bin WU ; Hong JIANG
Acta Pharmaceutica Sinica 2014;49(12):1739-1742
Hyperuricemia mice model was established with uricase inhibitor (potassium oxonate) and uric acids in serum were observed. Polydatin (5, 10, 20 mg · kg(-1)) and benzbromarone (16.7 mg · kg(-1)) were given ig for 7 d in mice. Kidney tissues were used to detect gene contents ofurate anion transporter 1 (URAT1), organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) by real-time-PCR. The results showed that polydatin and benzbromarone can significantly reduce uric acid in blood of hyperuricemia mice (P < 0.05), compared with the model group. URAT1, OAT1 and OAT3 contents of the kidney in hyperuricemia mice changed significantly (P < 0.05), compared with the blank group. Polydatin can significantly inhibit the changing trends in these genes induced by potassium oxonate in a dose-dependent manner, the difference was significant (P < 0.05), compared with the model group. Those indicated that polysatin could reduce the level of the serum uric acid through promoting uric acid excretion.
Animals
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Disease Models, Animal
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Glucosides
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pharmacology
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Hyperuricemia
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drug therapy
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Kidney
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drug effects
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metabolism
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Mice
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Stilbenes
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pharmacology
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Uric Acid
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blood
2.Blunted perception of symptoms of asthma.
Ju-hong SHI ; Jiang-na HAN ; Wen-bing XU
Chinese Journal of Pediatrics 2006;44(4):272-274
Asthma
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diagnosis
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Child
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Humans
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Perception
4.The effect and mechanisms of 20-HETE on myocardial ischemia reperfusion injury
Yong HAN ; Lirong GUO ; Deying KONG ; Hui JIANG ; Hong TIAN
Chongqing Medicine 2015;(32):4465-4468
Objective To investigate the effect of 20‐HETE on the isolated myocardial ischemia reperfusion injury and to ex‐plore its underlying mechanisms .Methods Experiments were performed in isolated rat hearts subjected to 35 min of ischemia fol‐lowed by 40 min of reperfusion in Langendorff preparations .HET0016 (1 μmol/L) and various concentrations (10 ,30 or 50 nmol/L) of 20‐HETE were infused 10 min before the onset of ischemia and throughout the reperfusion period .Cardiac hemodynamic changes and myocardial contractility were continuously recorded with the Powerlab /8P system .Myocardial infarct size was meas‐ured by TTC staining .The level of ROS and the protein carbonyl content were determined by DHE fuorescence and DNPH method , respectively .Results Perfusion with HET0016 significantly improved myocardial ischemia reperfusion injury reduction in cardiac contractility ,after inhibited the production of 20‐HETE significantly reduced the occurrence of myocardial infarction area (P<0 .05) ,but exogenous join 20‐HETE aggravated I/R‐induced myocardial injury (P<0 .05) .Myocardial ischemia reperfusion injury significantly increased production of ROS and oxidative stress ,both of which were significantly inhibited by HET 0016 and enhanced by 20‐HETE administration(P< 0 .05) .Conclusion 20‐HETE stimulates ROS production and enhance protein carbonylation , which aggravates myocardial ischemia reperfusion injury .
5.Survey of the quality of life of the aging population in rural areas in Chongqing
Feifei HAN ; Luyu XIANG ; Baoping ZHANG ; Hong XU ; Yanggang JIANG
Chongqing Medicine 2013;(29):3529-3531
Objective To evaluate of the quality of life of the aging population in rural areas of the underdeveloped areas of Chongqing ,and analyze its influencing factors ,provide a reference in order to improve their quality of life .Methods Hierarchical sampling was conducted ,474 rural aging people from Qianjiang District and Zhongxian were extracted ,Adopt second edition of the Life quality evaluation Scale (SF-36) was used to describe its quality of life each dimension of score ,the dimension of physical health and mental health dimensions influencing factors were analyzed .Results Among the total score of 474 Zhongxian rural quality of life of the aging population ,median M (interquartile QR) was 78 .00 (30 .00) ,and the physical health dimension M (QR) score was 75 .00 (35 .00) ,mental health dimension M (QR ) score was 76 .00 (20 .00) .Conclusion Their mental health concerns should be strengthen to improve the quality of life of the aging population the main way .
6.The anti-atherosclerotic effect and mechanism of puerarin
Shuang JIANG ; Yan-xing HAN ; Jian-dong JIANG ; Yu-hong WANG
Acta Pharmaceutica Sinica 2021;56(4):966-971
In recent years, with the improvement in living standards, the morbidity and mortality of cardiovascular and cerebrovascular diseases has increased markedly. Atherosclerosis is the main pathological basis for cardiovascular and cerebrovascular diseases, and there are many risk factors for atherosclerosis. The pharmacological effects of puerarin are broad, and considerable clinical data confirms that puerarin has a definite effect on cardiovascular diseases resulting from atherosclerosis. The use of puerarin for atherosclerosis has increased in recent years. This article reviews the effect and mechanism of puerarin on atherosclerosis.
7.Effects of nuclear factor-?B decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclampsia
Rong-Zhen JIANG ; Ya-Juan HUANG ; Jing-Hong GU ; Wei-Hong MU ; Han-Ping CHEN ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To study the effects of nuclear factor-?B(NF-?B)decoy oligodeoxynucleotide(ODN)on the preeclamptic umbilical serum induced expression of precollagen Ⅰ,Ⅲ mRNA and tumor necrosis factor-?(TNF-?)in cultured human umbilical artery smooth muscle cells (HUASMC).Methods Primary cultured HUASMC of normal pregnancy were divided into four groups: group A(HUASMC were incubated with umbilical serum of normal pregnancy);group B(HUASMC were incubated with umbilical serum of preeclampsia);group C(HUASMC were transfected with NF-?B cis decoy ODN 48 h before incubation with umbilical serum of preeclampsia);group D(HUASMC were transfected with NF-?B scramble ODN 24 h before incubation with umbilical serum of preeclampsia).NF-?B cis decoy ODN and NF-?B scramble ODN were transfected with cationic lipofectamine to the latter two groups,respectively.The proliferation of human umbilical artery smooth muscle cells was evaluated by methyl thiazolyl tetrazolium and the apoptosis was analyzed by flow cytometry.The expression levels of precollagen Ⅰ,Ⅲ mRNA were detected by RT-PCR,the expression levels of TNF-? were detected by western blot.Results(1)The proliferation of group B(0.19?0.02)and group D(0.18?0.03)was significantly increased as compared with those of group A(0.11?0.02)and group C(0.14?20.02)(P0.05).(5)The expression of TNF-? of group B(0.74?0.11),group C(0.36?0.09)and group D(0.79?0.12)were significantly higher than that of group A(0.15?0.03)(P0.05).Conclusions NF-?B cis decoy ODN could down-regulate the proliferation,as well as the expression levels of precollagen and TNF-? of HUASMC induced by umbilical serum of preeclampsia.NF-?B may play an important role in the pathogenesis of placental artery abnormalities in preeclampsia.
8.Diagnostic specificity of dynamic assessment and monitoring of spirometry in asthma.
Hong JIANG ; Jiang-Na HAN ; Wen-Bing XU ; Yuan-Jue ZHU
Acta Academiae Medicinae Sinicae 2009;31(4):488-493
OBJECTIVETo evaluate the diagnostic specificity of dynamic assessment and monitoring using a portable spirometer in diagnosis and differential diagnosis of asthma.
METHODSWe retrospectively reviewed the results of dynamic monitoring of spirometry in 145 symptomatic patients with physician-diagnosed asthma. Flow-volume curve and simultaneous symptoms and mood were measured in a fixed-time thrice-daily assessment schedule for 2 weeks. Patients were allowed to make additional measurements in case of symptom exacerbations.
RESULTSThe clinical data of 51 males and 94 females with a mean age of (39.1 +/- 13.0) years (ranged from 10 to 65 years) were analyzed. Duration of asthma before study was (6.7 +/- 9.9) years. Of 145 patients with physician-diagnosed asthma, 126 (87%) could be conclusively confirmed for a diagnosis of asthma. Asthma was misdiagnosed in 14 patients (9.7%). Overdiagnosis of asthma was observed in 5 patients (3.4%).
CONCLUSIONDynamic assessment and monitoring using a portable spirometer by revealing variability and reversibility of airway obstruction may provide an additional tool for diagnosis and differential diagnosis of asthma.
Adolescent ; Adult ; Aged ; Airway Obstruction ; Asthma ; diagnosis ; Child ; Diagnosis, Differential ; Diagnostic Errors ; Female ; Humans ; Male ; Middle Aged ; Peak Expiratory Flow Rate ; Spirometry ; methods ; Young Adult
9.Effect of integrated Chinese medical treatment (as maintenance therapy) on the survival time of patients with advanced non-small-cell lung cancer: a clinical study.
Ling-Shuang LIU ; Li-Ping SHEN ; Yi JIANG ; Zhi-Fen HAN ; Jian HONG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(5):526-530
OBJECTIVETo observe clinical effect of integrated Chinese medical (CM) treatment (as maintenance therapy) on the progression-free survival (PFS) and overall survival (OS) in patients with advanced non-small-cell lung cancer (NSCLC) after first-line chemotherapy.
METHODSThe study was a prospective, randomized, controlled clinical trial. Totally 69 non-progressive advanced NSCLC patients treated with first-line chemotherapy were randomly assigned to the test group (34 cases) and the control group (35 cases). Patients in the control group were treated with one Western drug chemotherapy (Gemcitabine or Alimta or docetaxel). Those in the test group were treated with integrated CM treatment (CM decoction, CM Intravenous preparation, and point application). Each cycle consisted of 21 days. Treatment lasted till the disease progressed, or intolerable toxic/adverse reactions occurred, or patients refused to continue the treatment. Patients' life spans were regularly followed-up.
RESULTS(1) The median cycle of maintenance therapy was 2 cycles for two groups with no statistical difference (P =0.274). The median PFS was 12.43 weeks in the test group and 10.00 weeks in the control group, showing statistical difference (P =0.025). The middle survival time (MST) was 18.8 months in the test group and 16.73 months in the control group, showing no statistical difference (P =0.437).
CONCLUSIONCM treatment (as maintenance therapy) showed quail effect to one Western drug chemotherapy in prolonging patients' life span.
Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Deoxycytidine ; analogs & derivatives ; therapeutic use ; Disease-Free Survival ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Pemetrexed ; therapeutic use ; Prospective Studies ; Taxoids ; therapeutic use
10.5-Azacytidine combined with angiotensin Ⅱ induces differentiation of adipose-derived mesenchymal stem cells into cardiomyocytes
Jing WANG ; Jiang-Hong HAN ; Qiong LI
Chinese Journal of Tissue Engineering Research 2017;21(29):4629-4634
BACKGROUND:Taking effective measures for myocardial necrosis develops a trend in the clinical treatment of myocardial infarction.OBJECTIVE:To investigate the mechanism of 5-azacytidine combined with angiotensin Ⅱ in the differentiation of mouse adipose-derived mesenchymal stem cells into cardiomyocytes.METHODS:Twenty ICR mice aged 4 weeks old were used to isolate adipose-derived mesenchymal stem cells using type Ⅰ collagenase digestion method.Cell morphology was observed,cell proliferation was detected using MTT,and immunofluorescence technique was used to detect the expression of CD90,CD105,CD73 and CD45.Passage 4 adipose-derived mesenchymal stem cells were cultured for 24 hours,then induced by 15 μmol/L 5-azacytidine or 15 μmol/L 5-azacytidine plus 0.1 μmol/L angiotensin Ⅱ for another 24 hours,and finally cultured in a complete medium.The expression of cTnT protein in myocardial cells was detected by western blot assay.RESULTS AND CONCLUSION:Primary adipose-derived mesenchymal stem cells with small nuclei were spindle-shaped or starlike.After passage,the cells were increased in size,with uniform shape and regular arrangement.Passage 3 mouse adipose-derived mesenchymal stem cells showed an S-shaped growth curve:days 1-3 was incubation period,the cells showed logarithmic growth at day 4,the cell growth reached the peak at days 5-7,and then entered stationary phase.The passage 4 cells were positive for CD90,CD105 and CD73,but negative for CD45.The expression of cTnT was significantly higher after cell induction with 5-azacytidine combined with angiotensin Ⅱ than 5-azacytidine alone.In conclusion,the combined use of 5-azacytidine and angiotensin Ⅱ is beneficial for the cardiomyocyte-like differentiation of adipose-derived mesenchymal stem cells.