Objective:To investigate the effects of siRNA-mediated Plk1 depletion on COS-7 cells and the therapeutic potential of Plk1 in cancer treatment.Methods:Small interfering RNA(siRNA) were used to specifically deplete Plk1 in COS-7 cells.RT-PCR and immol/Lunoblotting analysis were used to verify the inhibitory effect of siRNA against Plk1.Trypan blue exclusion assay and FACS analysis were conducted to determine the effects of Plk1 depletion on the growth and cell cycle profile of COS-7 cells.Results:Synthetic siRNA duplexes against Plk1 were introduced into COS-7 cells,which subsequently resulted in a significant inhibition in Plk1 expression in the cells.Cell growth assay indicated that siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells.FACS analysis revealed that Plk1 depletion caused cell cycle arrest,resulting into large population of sub-G1 cells.Conclusion:siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells suggesting the therapeutic potential of Plk1 in cancer treatment.

Objective:To investigate the expression and significance of NF-?B,and ICAM-1 in laryngeal carcinoma patients.Methods:Two groups of laryngeal carcinoma tissue (one group derived from the laryngeal carcinoma patients;the other from the normal persons) were detected for NF-kappa B P65 protein and ICAM-1′s expressions by immunohistochemistry staining.Results:(1)The expressions of NF-kappa B P65 protein and ICAM-1 of the laryngeal carcinoma patients were much more strengthened than those of the normal persons(P

Objective:To explore the expression and significance of IKK, I?B and NF-?B P65 of NF-? B signal transduction pathway in laryngeal carcinoma patients.Methods:The expression of IKK?, I?B?,NF-?B P65 was examined by immunohistochemical S-P method in laryngeal carcinoma tissues of 40 cases and normal laryngeal tissues of 20 cases.Results:The expression of IKK? and NF-?B P65 in laryngeal carcinoma tissue was higher compared with normal laryngeal tissue,while the expression of I?B? was lower( P

Objective:To test Jiang Guangrong's(2005)three-dimension model on the depth of counseling process.Methods:In this process-outcome research,28 sessions in a university counseling center were videotaped and coded in terms of the model.Structure Equation Model(SEM)was used to examine the relations between the coding and counselors and clients' evaluation of the session depth.Results:(1)Clients and counselors evaluated differently on the session depth,and clients' evaluation was more positive than counselors' [(4.95±1.12)vs.(4.23±0.94),P<0.05];(2)The model was significantly correlated with counselors' session depth evaluation(path coefficient = 0.55,P<0.01),but not with the clients';(3)The session curves describing each dimension changing over time had different patterns in sessions of different depth level,through which more about the counseling process could be known.Conclusion:The three-dimension model on the depth of counseling process has got some empirical evidences.

Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.

Guided by systematic material evaluation theory,the present study assessed textbooks of medical English listening and speaking published over the last 20 years,and conducted a relevant survey to investigate different opinions of the textbooks among teachers and students.It found that cognitive schema theory,information processing theory,the register theory and communicative principle were adopted as guidelines in the compilation.Multi-tasks were utilized to train language skills;medical terminology learning was put great emphasis on;original audio-visual materials were employed.Besides,the majority of the textbooks were compiled based on topics or situations.However,most textbooks of medical English listening and speaking miss the section for cultivating students' autonomous learning,independent thinking as well as phonetic exercise,and they lack the teacher's book and students' book,which should be improved soon.

Objective To describe the clinical features and observe the effect of vitrectomy on traumatic avulsion of vitreous base with giant retinal dialysis.Methods Seven cases with giant retinal dialysis(rang 120～150?) secondary to traumatic avulsion of vitreous base and PVR grade B to C 3 were retrospectively reviewed.All eyes had recieved vitrectomy,scleral buckle and lenses removed,and six eyeballs underwent postoperative sequential fluid-gas exchange.Results In most cases,the dialyses and avulsed vitreous base developed in the temponate quadrants and complicated lenses dislocation,pupilloparalysis,and vitreous hemorrhage.All cases attained success after disappearance of the intraocular tamponate.The major complication was elevated intraocular pressure,which was transient.Conclusions Giant retinal dialysis may result from traumatic avulsion of vitreous base.These dialysis often become clinical manifestation for several days or longer following ocular contusion.Vitrectomy is a very effective management for these cases.

Epilepsy is a family of neurological disorders that are common and harmful to human's health. The etiologies of epilepsy and the clinical manifestations of seizures are complex and various. To elucidate the mechanisms underlying seizure generation, lots of researches in the anatomy, physiology, biochemistry, genetic and molecular biology of epilepsy have been done over many years. However, no single hypothesis provides a unifying framework within which all kinds of epilepsy can be included so far. In fact, the electrophysiologic essence of epileptic seizure is abnormal excess discharges of neurons. On the standpoint of electrophysiology, the reasons of abnormal discharges of neurons are of common character: depolarizing currents with long durations and high amplitudes on cell levels, which is closely related to slow waves with high amplitudes on EEG levels. This can make us understand the mechanism of epileptic seizures better, and is helpful for well control and therapy of seizures.

0.5.8 factors can explain the 80% of the whole variable. There were significant differences in the aspects of emotion,ADL/IADL,moving ability,participating and the total score(P

Bone marrow mesenchymal stem cells(MSCs) are a sort of somatic stem cells deriving from the mesoderm.They are capable of self-renewing,differentiating into mesenchymal or non-mesenchymal tissues,anti-inflammatory,reducing oxidative stress and immunomodulatory.MSCs exert their protective effect on various tissues,especially the ischemia-reperfusion injured tissue.This review summarizes the repairative potential of MSCs on ischemia-reperfusion injury.