Objective To review the recent studies about sentinel lymph node biopsy in breast cancer. Methods The literatures in recent years on the history, concept, technique and clinical application of sentinel lymph node biopsy were reviewed and summarized.Results There was no unified method for sentinel lymph node biopsy. There was a wide range of detection rate and false negative rate.Conclusion Prospective multi center random clinical trials will help to evaluate the clinical application of sentinel lymph node biopsy.

Objective To explore the diagnosis and treatment of phyllodes tumor of the breast. Methods Clinical data from 30 patients with phyllodes tumor confirmed surgically and pathologically were analyzed retrospectively. Results Of the 30 cases of phyllodes tumor, there were 16 cases of benign tumor, 9 cases of borderline tumor and 5 cases of malignant tumor according to the standard histological criteria. Local mass excision, total mastectomy, modified radical mastectomy and tumor mass excision were performed in 8, 8 and 13 patients, and 1 patient, respectively. A follow up of 20 cases for 1-24 years found 1 death, but all the others survived. Four patients out of the 20 patients underwent total mastectomy or modified radical mastectomy due to tumor recurrence. Conclusion The preoperative diagnosis of phyllodes tumor is difficult. Intraoperative frozen section examination of the suspicious lesions should be conducted. Multiple slices of the specimen should be performed. Surgical options are closely associated with the prognosis. Wide local excision or total mastectomy is recommended in surgical options.

Objective:To observe the clinical effect of acupuncture combined with tuina manipulation for thoracic facet joint disorder. Methods:A total of 93 eligible cases were randomly allocated into an observation group and a control group. Patients in the observation group (n=46) were treated with acupuncture combined with tuina manipulation, whereas patients in the control group (n=47) were treated with tuina manipulation alone. The treatment was done once a day, for a total of 3 times. Therapeutic efficacies were then evaluated according to scores on signs and symptoms.
Results:After treatment, there were intra-group statistically significant differences in scores of eight signs and symptoms (all P<0.01); and the between-group differences were statistically significant in scores of eight signs and symptoms (all P<0.05). After three times of treatment, the recovery rate in the observation group was 54.4%, versus 25.4% in the control group, showing a statistical significance (P<0.05).
Conclusion:Acupuncture combined with tuina manipulation can obtain better effects than tuina manipulation alone for thoracic facet joint disorder and is therefore worth further clinical application.

Objective To investigate the prevalence and distribution of Yin and Yang deficiency syndrome on different nationalities in different parts of Xinjiang in order to offer epidemiology evidence for the research on the cause of dryness syndrome in northwest China.Methods The questionnaire was designed according to the Chinese medicine theories and the sampling questionnaire investigation in five regions including Hotan Xinjiang and Leshan Sichuan and Shanghai was carried out.Then a computation differentiation of Yin and Yang deficiency syndrome was done for the statistical analysis.Results Totally 5544 questionnaires were accomplished.In the five regions except Urumqi,the incidence of Yin and Yang deficiency syndrome in Xinjiang was higher than that of Shanghai and Sichuan(P

Objective To study the association of ER? expression with the expression of hormonal receptor and prognostic biomarkers.Methods The expressions of ER?,ER?,PR,Ki-67,HER-2/neu and p53 were detected in tissue samples from 109 breast cancer patients by immunohistochemistry on tissue microarray slides.The histologic grade,tumor size and lymph node metastasis status of 109 cases of breast cancer were recorded and served as comparison indexes.Results ER? was expressed in benign epithelium and stroma cells,and breast cancer cells in 59% breast cancer tissues of different histologic types.ER? was coexpressed with ER? in 45% breast cancer tissues.ER? expression was found a statistically significant association with HER-2/neu(P

Objective:To explore effect for isoproterenol rising detection rate of paroxysmal supraventricular tachy‐cardia by transesophageal atrial pacing (TEAP) .Methods :A total of 85 patients with suspected PSVT attack history received routine TEAP ( basic stimulate ) ,for those failed to evoke PSVT ,they received additional intravenous infusion of isoproterenol to make heart rate rise to 120 beats/min ,then TEAP was repeated .Results :Routine TEAP evoked PSVT in 29 cases (34.1% ,29/85) ;among the remaining 56 cases un - evoked PSVT ,after intravenous infusion of isoproterenol PSVT was evoked in 54 cases (96.4% ,54/56) by TEAP ,and this detection rate was significantly higher than that of routine TEAP (96.4% vs .34.1% , P = 0.039) .Conclusion :Intravenous infusion of isoproterenol can greatly raise the detection rate of paroxysmal supraventricular tachycardia by transesophageal atrial pacing .

Nuclear magnetic resonance (1H-NMR) fingerprint of Rhodiola rosea medicinal materials was established, and used to distinguish the quality of raw materials from different sources. Pulse sequence for water peak inhibition was employed to acquire 1H-NMR spectra with the temperature at 298 K and spectrometer frequency of 400.13 MHz. Through subsection integral method, the obtained NMR data was subjected to similarity analysis and principal component analysis (PCA). 10 batches raw materials of Rhodiola rosea from different origins were successfully distinguished by PCA. The statistical results indicated that rhodiola glucoside, butyl alcohol, maleic acid and alanine were the main differential ingredients. This method provides an auxiliary method of Chinese quality approach to evaluate the quality of Rhodiola crenulata without using natural reference substances.
Magnetic Resonance Spectroscopy ; methods ; Principal Component Analysis ; Rhizome ; chemistry ; Rhodiola ; chemistry

Objective:A study was conducted to observe and compare the efficacy and safety of endostar combined with peme-trexed in elderly patients with advanced lung adenocarcinoma. Methods:Sixty advanced lung adenocarcinoma (ⅢB-Ⅳ) patients who never received any therapy were included. The patients were divided into two groups. One group comprised endostar treatment com-bined with pemetrexed (26 cases of males, 15 cases of females, and 11 cases of individuals aged 65 years old to 78 years old), and the other group comprised pemetrexed only (34 cases of males, 20 cases of female, and 14 cases of individuals aged 65 years old to 78 years old). The two groups were treated for 4 to 6 cycles, and evaluation of treatments was performed every two cycles. Results:The endostar group was re-treated for 80 cycles, and the average cycle was 3.1. The group without endostar was re-treated for 115 cycles. The short-term effects are as follows. The total effective rates (RRs) in the experimental and control groups were 23.1%and 14.7%, re-spectively, and the difference was statistically significant (P<0.05). The disease control rate (DCR) was not significantly different (P>0.05). For pleural effusion, RR and DCR were significantly better in the experimental group than in the control group (P<0.05). In the experimental group, compared with PD, the microvessel density (MVD) in the DCR showed higher expression, and a statistically signif-icant difference (P=0.03) was observed. In the control group, compared with PD, the MVD in the DCR also showed higher expression, but no significant difference (P=0.73) was observed. The long-term effects were as follows: median progression-free survival (PFS), median survival, and side effects between the two groups were not significantly different (P>0.05). Conclusion: Endostar combined with pemetrexed showed increase in total efficiency in elderly patients with lung adenocarcinoma, and malignant pleural effusion was controlled without increasing the toxicity of chemotherapy. MVD can be used as a predictor of Endostar application.

The microbial populations and community structure characterization technologies of the enhanced biological phosphate removal system were reviewed comprehensively in this paper, and their future research directions were outlined.

AIM: To establish the quality standard for Xaozhi Tablets(Lasiosphaera seu Calvatia, Rhizoma Dioscoreae Cirrhosae, Radix Sophorae Tonkinensis, Concha Ostreae, etc.). METHODS: Rhizoma Dioscoreae Cirrhosae and Radix Sophorae Tonkinensis were identified by TLC. Matrine was determined by HPLC. RESULTS: Rhizoma Dioscoreae Cirrhosae and Radix Sophorae Tonkinensis could be detected by TLC. Matrine showed a good linear relationship in the range of 0.2040～2.040?g and the average recovery was 97.64%, RSD was 1.49%. CONCLUSION: The methods are simple, sensitive and accurate with good reproducibility. The methods may be used for the quality control of Xiaozhi Tablets.