Objective:To investigate the effects of siRNA-mediated Plk1 depletion on COS-7 cells and the therapeutic potential of Plk1 in cancer treatment.Methods:Small interfering RNA(siRNA) were used to specifically deplete Plk1 in COS-7 cells.RT-PCR and immol/Lunoblotting analysis were used to verify the inhibitory effect of siRNA against Plk1.Trypan blue exclusion assay and FACS analysis were conducted to determine the effects of Plk1 depletion on the growth and cell cycle profile of COS-7 cells.Results:Synthetic siRNA duplexes against Plk1 were introduced into COS-7 cells,which subsequently resulted in a significant inhibition in Plk1 expression in the cells.Cell growth assay indicated that siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells.FACS analysis revealed that Plk1 depletion caused cell cycle arrest,resulting into large population of sub-G1 cells.Conclusion:siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells suggesting the therapeutic potential of Plk1 in cancer treatment.

Objective:To explore the expression and significance of IKK, I?B and NF-?B P65 of NF-? B signal transduction pathway in laryngeal carcinoma patients.Methods:The expression of IKK?, I?B?,NF-?B P65 was examined by immunohistochemical S-P method in laryngeal carcinoma tissues of 40 cases and normal laryngeal tissues of 20 cases.Results:The expression of IKK? and NF-?B P65 in laryngeal carcinoma tissue was higher compared with normal laryngeal tissue,while the expression of I?B? was lower( P

Objective:To investigate the expression and significance of NF-?B,and ICAM-1 in laryngeal carcinoma patients.Methods:Two groups of laryngeal carcinoma tissue (one group derived from the laryngeal carcinoma patients;the other from the normal persons) were detected for NF-kappa B P65 protein and ICAM-1′s expressions by immunohistochemistry staining.Results:(1)The expressions of NF-kappa B P65 protein and ICAM-1 of the laryngeal carcinoma patients were much more strengthened than those of the normal persons(P

Objective:To test Jiang Guangrong's(2005)three-dimension model on the depth of counseling process.Methods:In this process-outcome research,28 sessions in a university counseling center were videotaped and coded in terms of the model.Structure Equation Model(SEM)was used to examine the relations between the coding and counselors and clients' evaluation of the session depth.Results:(1)Clients and counselors evaluated differently on the session depth,and clients' evaluation was more positive than counselors' [(4.95±1.12)vs.(4.23±0.94),P<0.05];(2)The model was significantly correlated with counselors' session depth evaluation(path coefficient = 0.55,P<0.01),but not with the clients';(3)The session curves describing each dimension changing over time had different patterns in sessions of different depth level,through which more about the counseling process could be known.Conclusion:The three-dimension model on the depth of counseling process has got some empirical evidences.

0.05 ; and (2) in combined test, the combined OR= 3.2353 and its 95% confidence interval was in 2.21 - 4.74 (? 2= 36.2 ,P

Bone marrow mesenchymal stem cells(MSCs) are a sort of somatic stem cells deriving from the mesoderm.They are capable of self-renewing,differentiating into mesenchymal or non-mesenchymal tissues,anti-inflammatory,reducing oxidative stress and immunomodulatory.MSCs exert their protective effect on various tissues,especially the ischemia-reperfusion injured tissue.This review summarizes the repairative potential of MSCs on ischemia-reperfusion injury.

Objective:To make a synthetic comment on treatment of children cerebral palsy with acupuncture in recent 10 years in China.Methods:Related papers were gotten from the journals between 1999-2008 in Chinese journal full-test database and VIP information-Chinese technology journal database by computer retrieval.The collected 143 papers were analyzed from the terms of clinical acupoints selection,treatment methods,time,and mechanism,and then summarized the clinical effects of acupuncture in treating children cerebral palsy.Results:The acupoints selection was focus on scalp acupuncture functional area and body points(including scalp points).Beside acupuncture treatment,the combination of acupoint embedding therapy,acupoint injection,and massage were also used.The therapy time was different from 20min to 4h,and the treatment course was usually be counted by month.The effects of acupuncture treatment were considered to be significant through improving brain circulation and recovering brain cells' function. Conclusion:Acupuncture treatment had advantages in improving the child'limbs function,nevertheless further studies on the research methods,study design and mechanism were all needed.

Objective To evaluate the clinical application value of percutaneous lung biopsy by CT fluoroscopy.Methods we analysed 31 cases of percutaneous lung biopsy by CT fluoroscopy which were proved by operation or pathology,with the puncture needle produced by Cook Co..The main point are: choose the best layer,point,angle,depth,draw and obtaining at many points.Results In these 31 cases,accurate diagnosis is 29 cases,include 9 cases of adenocarcinoma,6 cases of SqCa,2 cases of small cell carcinoma,1 case of metastatic carcinoma,1 case of alveolar cell carcinoma,5 cases of TB,4 cases of infection,1 case of inflammatory pseudotumor.The diagnosis accurate rate is 93.5%.2 cases were not diagnosed accurately,2cases of pneumothoras,complication rate is 6.5%.Conclusion The percutaneous lung biopsy by CT fluoroscopy was high diagnosis accurate rate,less of complication,this is a safe,effective,simple and practical way for diagnosing lung diseases.

Objective:To observe the effects of endocrine mechanism on uterine contraction on late pregnancy rats by applying acupuncture on KI6 using"open-close"needling time and"host-guest"needling sequence according to eight methods of the intelligent turtle.Methods:32 pregnant rats were divided into"open/host-guest"group(OHG group),"open/guest-host" group(OGH group),"close/host-guest"group(CHG group)and pregnant control group(PC group).8 healthy grown-up female rats were made into un-pregnant control group(UPC group).Firstly,open KI6 time was calculated.OHG group and OGH group were needled during the KI6 open time but CHG group was needled during the KI6 close time.Host-acupoint KI6 and guest-acupoint LU7 were needled for 5 minutes separately.After this,LI4 was needled for 20 minutes and then SP6 for 5 minutes.The contents of serum Estradiol(E2),Progesterone(P),ThromboxanceB 2(TXB 2)and 6-Keton-ProstaglandinF 1?(6-K-PGF 1?)were measured. Results:Compared with UPC group,the contents of serum P in OHG group increased significantly(P

Objective To study the relationship between CA125 and endometriosis.Methods We tested the level of CA125 in serum and fluid of ovarian cysts of patients with endometriosis and non-endometriosis,respectively.Results The mean CA125 concentration in the fluid of endometrial cysts of the ovary(ECO) was remarkably higher than that of the serum in ECO group(P0.05).Conclusion The level of CA125 has bearing on the occurrence,development and prognosis of endometriosis,CA125 level is positively correlated with the severity of the disease.Therefore,it is valuable to test serum CA125 level for diagnosing endometriosis.