OBJECTIVE:To improve the quality standard for Jingwu oral liquid.METHODS:The qualitation of Radix Polygoni Multiflori preparata and solomonseal rhizome in Jingwu oral liquid were identified by TLC and the assaying of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside in the oral liquid were determined by HPLC.RESULTS:In the TLCs of Radix Polygoni Multiflori preparata and solomonseal rhizome,the color of speckles was the same as that of the control articles in the corresponding places.The linear range of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside was 0.0 562?g～0.6 744?g(r=0.9 996);The average recovery was 100.1%(RSD=1.89%).CONCLUSION:The method is simple,fast,and reproducible and of high recovery rate,which can be used as quality control for Jingwu oral liquid.

Objective:To observe the efficacy of Chuankezhi injection combined with budesonide and formoterol fumarate powder for inhalation in the treatment of chronic obstructive pulmonary disease ( COPD) . Methods:Totally 120 patients with COPD were ran-domly divided into two groups:the observation group inhaled budesonide and formoterol fumarate powder for inhalation combined with intramuscular injection of Chuankezhi injection, and the control group inhaled budesonide and formoterol fumarate powder for inhala-tion. The treatment course was 28 days. The pulmonary function indices [ FEV1% expected value, FEV1 /FVC, 6 min walking dis-tance (6MWD)], the times of acute exacerbation and the adverse drug reactions during the treatment were observed and compared be-tween the two groups. Results:After the treatment, the lung function and 6MWD were improved in the two groups, and the improve-ment of FEV1% expected value in the treatment group was more obvious than that in the control group [(59. 7+12. 1)% vs (49. 8+11. 3)%, P<0. 05]. The number of patients with at least once of acute exacerbation was significantly reduced in the treatment group when compared with that in the control group (35% vs 62%, P<0. 01). The incidence of adverse drug reactions in the two groups showed no significant difference. Conclusion:Chuankezhi injection combined with budesonide and formoterol fumarate powder for in-halation can notably improve the lung function in the patients with COPD and reduce the times of acute exacerbation without effect on the incidence of adverse drug reactions.

Objective To investigate hepatitis B virus surface antign (HBsAg) quantitative value quantitatively in serum of chronic hepatitis B virus (HBV) infection patients with different clinical stages,at the same time,to explore the correlation between HBV DNA,patient's age and HBsAg quantitative values.Methods Collected 774 cases without antiviral treatment of chronic HBV infection from our hospital,according to the clinical features,divided cases into six groups:chronic HBV carrier group (102 cases),inactive HBsAg carrier group (211 cases),hepativis B virus e antigen (HBeAg) positive chronic hepatitis B group (236 cases),HBeAg negative chronic hepatitis B group (114 cases),HBeAg positive hepatitis B cirrhosis group (52 cases),HBeAg negative hepatitis B cirrhosis group (59 cases).Used chemiluminescence immunoassay particles analysis to determine HBsAg quantitative value in serum in patients,used real-time fluorescent quantitative polymerase chain reaction method to determine HBV DNA quantitative value in patients,and then compared differences among groups.Results HBsAg quantitative value from high to low respectively were chronic HBV cartier group,HBeAg positive chronic hepatitis B group,HBeAg negative chronic hepatitis B group,inactive HBsAg carrier group,HBeAg negative hepatitis B cirrhosis group,HBeAg positive hepatitis Bcirrhosis group,the median quantitative value of HBsAg were [7.80 (6.69-8.32),7.11 (5.42-8.27),6.57 (5.66-7.53),6.38 (4.39-7.40),6.22 (4.84-6.91),6.13 (5.48-7.01)] ; positive correlation was found between HBsAg quantitative value and HBV DNA in HBeAg positive chronic hepatitis B group and HBeAg negative chronic hepatitis B group (r =0.714,0.390,P ＜ 0.01); negative correlation was found between HBsAg quantitative value and age in chronic HBV infection(r =-0.416,P ＜ 0.01) ; Monitored HBsAg quantitative value of inactive HBsAg carriers after the age 40 had important clinical value.Conclusions HBsAg quantitative value is different in the various phases of chronic HBV infection.HBV DNA levels and age are related with HBsAg quantitative value.HBsAg quantitative value should be monitored in inactive HBsAg carriers after the age 40.

Pituitary tumors, originated from homoral adenohypophyseal cells, are very common in intracranial neoplasms. They are characterized by a wide range of biological behavioral related to hormoral and proliferative activities. Previous pituitary adenoma studies mainly devoted to histological and ultrastructural classification with immunohistochemical techniques. Molecular biology allowed new approaches, especially concerning the pathogenesis and progression. This article reviews the progress of these researches regarding chromosomes, oncogenes, antioncogenes, transgenic animal models and genetic therapy.

Objective: To study the quality standard of Fangtuoshengfaling Lotion (Radix et Rhizoma Rhei, Radix Sophorae Flavescentis, Radix Polygoni Multiflori, Radix Angelicae Sinensis, Radix Astragali seu Hedysarion, etc.). Methods: Radix et Rhizoma Rhei, Radix Sophorae Flavescentis were identified by TLC. The content of 2,3,5,4′ tetrahydroxystibene 2 O ? D glucoside in Radix Polygoni Multiflori of lotion was assayed by HPLC. Results: The linearity of 2,3,5,4′ tetrahydroxystibene 2 O ? D glucoside was found in the range from 0.36～1.84ng. The average recovery was 96.866% and RSD =0.898%( n =5). Conclusion: The method was found to be simple, accurate and with a good reproducibility and may be used for the quality control of the Lotion.

Circulating tumor markers have been paid more attention in the application of the treatment for breast cancer, the level of which has extended from protein to gene, including traditional tumor markers, HER-2 extracellular domain, circulating tumor cells, circulating tumor DNA (ctDNA), circulating RNA (ctRNA) and so on. As “liquid detection”, the detection of circulating tumor markers with real-time dynamic, easy operation, good reproducibility and other advantages are widely used in aiding early diagnosis, determining prognosis, prospectively predicting response or resistance to speciifc therapies, surveillance after primary surgery, and monitoring therapy in patients with advanced disease, The further study of circulating tumor markers may contribute to patient’s individual treatment.

Objective To observe the clinical effect of card other tromethamine for postpartum hemorrhage. Methods 80 cases with postpartum hemorrhage were randomly divided into the observation group and control group, 40 cases in each group.The control group was given oxytocin treatment.While the observation group was given oxyto-cin and card other tromethamine treatment.The clinical curative effect,bleeding time after treatment,postpartum 2h haemorrhage amount and adverse reaction,etc.were observed and compared.Results The total effective rate of the observation group was 95.0%,which was significantly higher than 75.0% of the control group,the difference was sta-tistically significant(χ2 =15.68,P <0.05).The haemorrhage amount of postpartum 2h of the observation group was (273.7 ±10.5)mL,which was significantly lower than (547.1 ±16.4)mL of the control group,the difference was statistically significant(t =88.79,P <0.05).The bleeding time of the observation group was (14.8 ±3.4)min,which was significantly shorter than (34.9 ±8.4)min of the control group,the difference was statistically significant (t =14.02,P <0.05).The incidence rate of adverse reactions of the observation group was 7.5%,that of the control group was 5.0%,there was no statistically significant difference between the two groups(χ2 =0.53,P >0.05 ). Conclusion Card other tromethamine in the treatment of postpartum hemorrhage has good clinical curative effect and high safety.

Objective To investigate the effect of acceptance and implementation therapy on anxiety and depression in cancer patients'spouse. Methods The spouses of 50 hospitalized patients from January to June 2014 were assigned into the control group and the spouses of another 50 cases of hospitalized patients from July to December 2014 into the observation group . All the patients received routine care methods. The control group received general health education and the spouses in the observation group were managed with acceptance and implementation therapy, totally for 3 times. After the intervention, the self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used to evaluate the levels of anxiety and depressions in both groups. Results Before intervention, there were no differences in SAS and SDS scores between the groups (P>0.05). After intervention, the SAS scores in the observation group and the control group scores were (48.36 ± 6.28) and (55.11 ± 6.74), respectively, with statistically significant difference (t=7.530, P<0.05). The SDS scores of the observation group and control group were (50.03 ± 4.12) and (58.50 ± 4.63) respectively, with statistically significant differences (t=6.672, P<0.05). The SAS and SDS score of observation group were lower than those before intervention (t = 7.283,7.524, all P < 0.05). Conclusion The acceptance and implementation therapy can effectively alleviate the anxiety and depression in the spouses of those patients with cancer.

Objective To analyze the composing principles of the prescriptions for Lijie disease by using The Prescription of Traditional Chinese Medicine Dictionary. Methods The prescriptions for Lijie disease in the book of The Prescription of Traditional Chinese Medicine Dictionary were collected. Database was established by using TCM Inheritance Support System (V1.1). Medicine using frequency, composing principles, and composition of new medicine were analyzed through association rules and entropy clustering method. Results Based on the analysis on 187 cases of prescriptions, 42 Chinese herbal medicines were with the frequency of 42 times and Angelicae Sinensis Radix was the core herbal medicine. 69 core combinations with three to four herbal medicines and 6 new prescriptions were obtained through evolution. Conclusion According to the analysis of composing principles and clustering new prescriptions of Lijie disease, the treatment for Lijie disease is toning liver and spleen, and nourishing liver and kidney.

Objective: To observe the effect of peptisorb on the result of intestinal preparation,the change of correlated nutritional index,the fuction of intestine and the immune function after operation in perioperative colorectal cancer patients.Methods: Sixty patients were randomly allocated into two groups. The trial group (n=30) received peptisorb 3days before and 7days after operation. Control group (n=30) received coloclysis treatment.The the level of Alb, globulin,the number of IgG, IgA,IgM, CD3+,CD4+/ CD8+ ,the station of intestinal mucosa and intestine cleaning during operation, nutritional state after operation, immune function, infcetious complications, length of stay were detected.Results: There were no significant difference in the station of intestinal mucosa and intestine cleaning. The index of nutritional and immune in two groups were descend, but the trail group was better(P＜0.01). The number of IgG,IgA, IgM, CD3+ in the trail group were significantly higher than the control group(P＜0.01),the rate of postoperative infectious complications in the trail group was lower than the control group.The trail group aslo showed shorter average length of stay after surgery than the control(P＜0.01).Conclusion: The peptisorb can ameliorate the host immune function and improve clinical outcome,which is a good nutrition support pathway to perioperative colorectal cance patinets.