3.Comparison of Two Methods for Endoscope Sterilization in Operating Room
Pinger ZHU ; Guijuan JIANG ; Xiaoming HUANG ; Yun SHEN
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To investigate which method for endoscope sterilization more convenient,qualified and with effectively elongating lifetime of endoscope usage.METHODS We used low temperature plasma hydroxide peroxide sterliization and 2% glutaraldehyde solution to sterilize endoscope and compared their effects.RESULTS Two methods were qualified for the requirements of serilization.Endoscope sterilization time with glutaraldehyde solution and low temperature plasma hydroxide peroxide was 10h and 55min,respectively.Sterilization-related endoscope attrition rate was 0.17% and 0,respectively,and showed significant difference(P
4.Hand Hygiene Compliance among Medical Staff:An Investigation
Pinger ZHU ; Guijuan JIANG ; Xiaoming HUANG ; Yun SHEN
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To find out the hand hygiene compliance among medical staff of our hospital,and discuss how to reduce the hospital infection.METHODS The questionnaires designed by ourself were used to observe the hand hygiene compliance on the scene and the hand hygiene knowledge among 120 Medical persons from 30 clinical departments and offices.RESULTS 81.7% Of the medical staff had accepted the education of the hand hygiene knowledge;89.2% of the medical staff considered washing hands could reduce the hospital infection.Only 2.5% of them considered hand hygiene had no relation to clinical treating result.30.8% Of medical staff washed their hands before contacting patients and 49.2% of them washed their hands after contacting patients.It showed significant difference between the two groups(P
5.Characteristics of Hospital Infection and Bacterial Resistance in Neurosurgery Ward
Pinger ZHU ; Guijuan JIANG ; Xiaoming HUANG ; Yun SHEN
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To study characteristics of hospital infection and bacterial resistance in our neurosurgery ward to make us reasonable antibiotics use and supply the scientific basis for hospital infection control.METHODS The data of 1100 inpatients in our neurosurgery ward in 2007 were analyzed prospectively and retrospectively.RESULTS The hospital infection rate was 11.91%,the case-time infection rate 12.55%,the lower respiratory tract infection rate 69.57%,the G-infection rate 54.44%,the G+ infection rate 30%,and the fungus infection rate was 15.56%,These pathogens were resistant to antibiotics in various degree.CONCLUSIONS It is significant to continuously monitor hospital infection and bacterial resistance in our neurosurgery ward to guide reasonable antibiotics use and control hospital infection effectively.
6.Sequence analysis of VP1 and VP4 genes of enterovirus 71 strains isolated from severe and mild hanD-foot-mouth disease cases in Shanghai, China, 2011
Yun CAI ; Huiguo SHEN ; Zhijun LI ; Liwen JU ; Lufang JIANG ; Haiyan XIONG ; Qingwu JIANG
Chinese Journal of Infectious Diseases 2013;(1):12-18
Objective To analyze the VP1 and VP4 genetic region of enterovirus 71 (EV71)isolated from severe cases and mild cases with hanD-foot-mouth disease (HFMD) in Shanghai in 2011.Methods Five EV71 strains isolated from severe cases and five EV71 strains from mild cases in 2011 were included.Reverse transcription-polymerase chain reaction (RT-PCR) method was used to amplify and sequence the VP1 and VP4 genes of EV71,and then the sequencing results were compared with those of A,B,C genotype reference EV71 strains from GenBank by nucleotide alignment,amino acid alignment and phylogenetic tree analyses.Results The homogeneity between EV71 strains from severe cases and mild cases was 96.0%-98.1% and 93.7%-99.5% for VP1 and VP4 nucleotide sequences,respectively.The VP1 nucleotide sequences of 5 strains isolated from severe cases and 5 strains from mild cases in Shanghai shared 86.9%-98.2% and 87.4% 98.5% identity with genotype C,respectively,while the homogeneity of VP4 nucleotide sequence was 85.5%-100.0%and 84.5%-99.5%,respectively.In addition,compared with the Fuyang EV71 strains (representative of C4 subtype),the strains from mild and severe cases shared homogeneity of 97.0%-98.2% and 97.9%-98.5% for VP1 gene,respectively,96.1%-100.0% and 97.1%-99.5% for VP4 gene,respectively.Among 3 strains isolated from severe cases,mutations at the residue 282 in the VP1 protein (N→S) and residue 7 in the VP4 protein (T→A) were discovered simultaneously.Conclusions The 10 EV71 strains isolated from severe and mild cases in Shanghai belong to subgenogroup C4.Among 3 strains isolated from severe cases,mutations at the residue 282 in the VP1 protein (N→S) and residue 7 in the VP4 protein(T→A) are discovered simultaneously.
7.Clinical comparative study on the treatment characteristics of secretory otitis media between cleft and non-cleft palate patients.
Sen LI ; Hong ZHANG ; Yun WEI ; Xilei ZHANG ; Yingru WU ; Jiang QIAN ; Liang SHEN ; Zhengjian ZHANG
West China Journal of Stomatology 2015;33(3):259-262
OBJECTIVETo discuss the treatment characteristics of secretory otitis media (SOM) in cleft palate children.
METHODSA total of 319 patients (524 ears) with SOM and cleft palate (3-14 years old) who accepted treatment were divided into experiment group A, group B, and group C according to effusion characteristics in the middle ear and tympanic pressure. Group A included 112 patients with serous effusion (198 ears). Group B included 162 patients with mucinous effusion (248 ears). Group C included 45 patients (78 ears) with negative pressure in the middle ear without effusion and an acoustic immittance. A total of 208 patients (246 ears) with SOM and tonsil and adenoid hypertrophy were divided into control group Al, group B1, and group Cl matched with the same effusion characteristics in the middle ear and tympanic pressure. Group A and Al accepted puncture in the tympanic cavity, group B and B1 accepted tympanostomy tubes, and group C and Cl accepted puncture in the tympanic cavity after palatoplasty, adenoidectomy, and tonsillectomy. All groups were treated with antibiotics and ear drops. Cure rate and recurrence rate between the experiment group and the control group were compared.
RESULTSThe control group had a better cure rate [93.09% (229/246)] than the experiment group [77.29% (405/524)] 12 months after treatment. The experiment group had a higher recurrence rate [14.57% (59/405)] than the control group [3.93% (9/229)]. Statistical differences were observed between the two groups (P<0.05). SOM with cleft palate initially had a low cure rate, and thus it was treated repeatedly for many times.
CONCLUSIONSOM with cleft palate is different from normal otitis media in terms of clinical manifestation, treatment, outcome, and prognosis. This case should be considered a special otitis media to be treated with special examination and therapy to obtain better results. Repeated puncture in the tympanic cavity and tympanostomy tubes for six months according to effusion characteristics are better treatment options for patients with SOM and cleft palate.
Child ; Cleft Palate ; Humans ; Middle Ear Ventilation ; Otitis Media with Effusion ; therapy ; Prognosis ; Recurrence
8.Effect of Bioimprinting by Lauric Acid on Esterification Activity of Lipase
Xiong-Wen CAO ; Lei SU ; Qi-Shen CHEN ; Tao LIU ; Yun-Jun YAN ; Jiang-Ke YANG ;
China Biotechnology 2006;0(07):-
Bioimprinting is a new developed technique to improve the characteristics of enzymes.Bioimprinting by lauric acid was conducted to improve the esterification activity of lipase PS in sol-gel immobilization process with methyltrimethoxysila(MTMS) and tetramethoxysila(TMOS) as the precursors.Results generated by checking the esterification activity and scanning electron microscope showed that bioimprinting can enhance the specific activity and thermal stability of lipase PS.The bioimprinting system was optimized by orthogonal experiment,and the optimal condition for lipase bioimprinting is water/silane molar ration(R) 12,polyethylene glycol(PEG) 120?l,and lauric acid 0.15 mmol.Compared with the free enzyme and the non-imprinted enzymes,the specific activity of imprinted enzymes has been improved 44.3 fold and 2.4 fold,respectively.Imprinted lipase show better thermal stability,and the relative activity is 58% after incubated in 80 ℃ for 0.5 h,while no activity was detected for the free enzyme.
9.The effects of swimming on neurotrophin-3 levels in the skeletal muscles of diabetic rats
Hongwei LI ; Zhongli JIANG ; Zhenhai SHEN ; Yun LU ; Yawen WU ; Ling CHEN
Chinese Journal of Physical Medicine and Rehabilitation 2010;32(4):241-244
Objective To explore the effects of exercise on the levels of neurotrophin-3 (NT-3) in the skeletal muscles of streptozotocin-induced diabetic rats. Methods The rats were divided into an 8-week exercise group (A), a 4-week exercise group (B), a diabetes control group (C), an exercise group (D) in which no dia-betes was induced, and a control group (E). The rat model of diabetes was induced in the rats of groups A, B and C by intra-abdominal injection of streptozotocin (STZ) at 55 mg/kg. The exercising rats were forced to swim for 60 minutes once daily, 5 days a week. The levels of NT-3 in skeletal muscles were measured with an enzyme-linked immunosorbent assay. Cadual nerve conduction velocity (CNCV) in all of the rats was evaluated at the beginning, and after 4 and 8 weeks of swimming exercise. Results NT-3 levels in the skeletal muscles in group C were sig-nificantly lower than in groups A, D and E. There was no statistically significant difference in NT-3 levels between groups B and C. The NT-3 levels showed a significant positive correlation with CNCV at the 8th week. Conclu-sions The increase in NT-3 levels of skeletal muscles induced by exercise could contribute partially to the im-provement of diabetic neuropathy.
10.Establishment functions and processes in the Wound Care Center
Qixia JIANG ; Xiaohua LI ; Yun LIU ; Ping SHEN ; Wei XU ; Guiling WANG
Journal of Medical Postgraduates 2003;0(12):-
Objective: To investigate the reasonable establishment of functions and processes in the Wound Care Center,and discuss the duties and roles of its enterostomal therapists(ET).Methods: According to the army's related infection management rules and nosocamiol infection control criteria,our Wound Care Center is divided into 8 areas,each with its set function and corresponding equipment and materials.We have laid out the consulting process for patients,stoma and wound management processes for nurses,responsibilities for ET and an operation mode for the Wound Care Center.The targets attained in work,teaching and research in the previous two years were analyzed and compared.Results: The index of the attainment of the tangets increased dramatically,with a growth rate of more than 20% in 8 of the 9 targets,most obviously in teaching and research.Conclusion: The division of the functional areas has proved to be reasonable,each operational process capable of meeting patients' needs and technical requirements,which enables ET to play important professional roles in the specialized field.