Objective:To evaluate clinical significances of the immuno pathogenic type of recurrent spontaneous abortion (RSA) Methods:Immunological analysis was made in ABO group antibodies by indirect hemagglutination, anti zona pellucida antibodies by BA ELISA, anti phospholipid antibodies by ELISA, blocking effect (MLR BE) by blocking test of one way mixed lymphocyte reaction, cytotoxic index (CI) by complement dependent micro cytotoxic test, and anti idiotypic antibodies to blocking antibodies (BE Ab2) by MLR BE inhibiting test Results:204 cases of RSA were classified into 4 types on the basis of Immunological analysis ,including typeⅠ (lack of materno fetal immuno recognition), typeⅡ(materno fetal inappropriate immuno recognition), typeⅢ(disorder of materno fetal immuno recognition), type Ⅳ (unexplained) TypeⅠand type Ⅲshowed mainly lack of blocking antibodies, and accounted for 85 8% in population of RSA Comparing to typeⅠand typeⅣ, typeⅡand typeⅢ appeared to abnormal increase in auto antibodies and iso antibodies to incompatible blood group Conclusion:Immuno pathogenic classification of RSA may be of an important significance to choosing the clinical immuno therapeutics and improving treating effect

Objective: To discuss the curative effect of trace hormone combined with antibacterial drugs on bacterial keratitis. Methods:Totally 80 cases of patients with bacterial keratitis were selected and divided into the observation group and the control group at random. The patients in the two groups were given medical treatment with sensitive antibiotics, while the patients in the observation group were additionally given trace cortisol. The clinical curative effect and vision recovery time in the two groups after the 3-week treatment were observed, and the occurrence of complications in the 2-month following-up after the medical treatment was compared as well. Results:After the 3-week medical treatment, the clinical effective rate and vision recovery effective rate in the observation group were much higher than those in the control group (P<0. 05). In the 2-month following-up after the medical treatment, the occurrence of complications in the observation group was much lower than that in the control group (P<0. 05). Conclusion:Trace hormone com-bined with antibacterial drugs has favorable curative effect on bacterial keratitis, which can effectively enhance the healing of helcoma, improve the vision recovery and reduce the occurrence of complications.

Together with Chinese mainstream ideology of equality between men and women,feminism has been profoundly impacted on women's bioethical attitudes and behaviors in contemporary Chinese society.The tension between women's pursuing for physical beauty and survival lasting shows that,under the influence of feminism,women have been out of attachment to men and gained equality with men and the independence,autonomy of life,but there will still be potential crisis of Bioethics.Therefore,referring to female bioethical issues,close attention must be paid to making use of the positive impact to feminist consciousness of feminism,and meanwhile prevent the distortion of feminist consciousness and crisis on Bioethics.

Objective To analyze the effect of the application of PDCA circulation in the management of oral instruments.Methods From January to June 2015,the head nurses analyzed problems of instruments management according to the outpatient instrument management and requirement standards in the department of stomatology.They followed the PDCA circulation principle,put a continuous improvement,and compared the changes in the management of oral instruments before and after 4 months (from March to June and from July to October) Results After the implementation of the PDCA circulation,the abnormal loss of equipment,numbers of instrument loss and equipment failure rate had significantly decreased,while equipment utilization rate had significantly increased,with significant statistical differences (P ＜ 0.05) Conclusion The application of PDCA circulation in the management of oral instruments could help reduce the problems of abnormal waste,loss,failure and so on,resulting in improving the instrument utilization rate.

OBJECTIVETo study the effect of Buyang Huanwu Decoction (BHD), Xuefu Zhuyu Decoction (XZD), and Sijunzi Decoction (SD) contained serums on expressions of Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB signals, lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), tumor necrosis factor-α (TNF-α), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and to explore possible anti-atherosclerotic mechanisms.

METHODSTwenty New Zealand rabbits were divided into 4 groups at random, i.e., the normal control group, the BHD group (6.7 g/kg), the XZD group (3.6 g/kg), and the SD group (1.6 g/kg), 5 in each group. All medication lasted for 7 successive days. Two h after the final medication, about 50 mL blood was withdrawn from rabbit heart for preparing serums. Human umbilical vein endothelial cell ECV304 were cultured in vitro for 18 h and randomly divided into the blank control group, the model group, the Western medicine (WM) control group, the BHD group, the XZD group, and the SD group at random. ECV304, except in the blank control group, were stimulated with lipopolysaccharide (LPS) for 2 h. Those in the WM control group and CM groups were treated respectively with corresponding CM contained serum for 24 h. Finally gene and protein expressions of TLR4, myeloid differentiation factor 88 (MyD88), tumor necrosis factor receptor-associated factor-6 (TRAF-6), NF-κB, LOX-1, TNF-α, ICAM-1, and VCAM-1 were detected by fluorescent quantitative PCR and Western blot.

RESULTSCompared with the blank control group, mRNA expressions of TLR4, MyD88, TRAF-6, NF-KB, LOX-1 , TNF-cx, ICAM-1, and VCAM-1 increased significantly; protein expressions of TLR4, NF-κB, LOX-1, TNF-α, ICAM-1, and VCAM-1 also increased significantly in the model group (P < 0.01). Compared with the model group, mRNA and protein expressions of each index could be significantly inhibited in the BHD group, the XZD group, and the WM control group (P < 0.05). Besides, mRNA and protein expressions of each index could be significantly elevated more in the BHD group and the XZD group than in the WM control group (P < 0.05). No statistical difference existed in each index between the SD group and the rest groups (P > 0.05).

CONCLUSIONSThe mechanism of BHD and XZD for fighting against atherosclerosis might be associated with inhibiting TLR4/NF-κB signal transduction pathway and expressions of its downstream inflammatory factors such as LOX-1, TNF-α, ICAM-1, and VCAM-1. But SD showed no associated effect on atherosclerosis.

Animals ; Atherosclerosis ; drug therapy ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Endothelial Cells ; Intercellular Adhesion Molecule-1 ; metabolism ; Lipopolysaccharides ; Myeloid Differentiation Factor 88 ; metabolism ; NF-kappa B ; metabolism ; Rabbits ; Scavenger Receptors, Class E ; Signal Transduction ; TNF Receptor-Associated Factor 6 ; metabolism ; Toll-Like Receptor 4 ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism ; Umbilical Veins ; Vascular Cell Adhesion Molecule-1 ; metabolism

Objective To boserve the effects of sophoridine on central nervous system in mice. Methods Behavioral methodology was employed to observe the effect of sophoridine on the normal mouse autonomic activities, and sleeping latency and sleep-lasting time of mice under threshold dosage of pentobarbital sodium. And to observe the synergistic effects of inducing convulsion between sophoridine and pentylenetetrazol, nicotin, strychine, isoniazid, and picrotoxin by subthreshold dosage. Results Sophoridine ip administrated (10 and 20 mg/kg) made the autonomic activities of mice suppressed by the rate of 66.9% and 76.6%, the sleeping latency of mice given pentobarbital sodium (40 mg/kg) prolonged, and sleeping time shortened (P

OBJECTIVE To investigate the risk factors of nosocomial infection in elderly lung cancer inpatients, to determine reasonable protective and therapeutic measures. METHODS Sixty-eight nosocomial infection cases and 215 non-nosocomial infection cases in elderly lung cancer inpatients were analyzed retrospectively. RESULTS The data of the TNM stage (?2=14.883,P=0.001), pathologic type (?2=34.217,P=0.000) and pathogenic site (?2=15.288,P=0.000) showed significant differences. By Logistic regression analysis, ECOG, antimicrobial treatment, hospitalization and hypoproteinemia showed significant differences(P

Objective To evaluate the performance of T-SPOT.TB Test and serological tuberculosis antibody(TB-Ab) in tuberculosis-assisted diagnosis.Methods Patients were diagnosed as tuberculosis from 2015 January to 2016 March in the hospital.T-SPOT.TB and tuberculosis antibody detection were performed for those patients.Results In 112 cases of tuberculosis patients,101 cases of T-SPOT.TB test were positive and the sensitivity was 90.18%,32 cases of tuberculosis antibody detection were positive and the sensitivity was 28.57%.In 41 cases of pulmonary tuberculosis,T-SPOT.TB assay showed positive results in 40 patients and the sensitivity was 97.56%,tuberculosis antibody detection showed positive results in 18 patients and the sensitivity was 43.9%;in 71 cases of extrapulmonary tuberculosis,T-SPOT.TB assay showed positive results in 61 patients and the sensitivity was 85.91%,tuberculosis antibody detection showed positive results in 14 patients and the sensitivity was 19.7%.Conclusion T-SPOT.TB was superior to serological tuberculosis antibody(TB-Ab),and the diagnosis of active tuberculosis was higher,and the sensitivity of T-SPOT.TB was higher than that of T-SPOT.TB value.

Objective To evaluate the effect of combination of phacoemulsification and trabeculectomy on patients with cataract and glaucoma.Methods 19 patients(23 eyes)with co-existing glaucoma and cataract were treated with triple procedure by means of scleral tunnel flap.The mean follow-up period was(15.8 ±3.5)months.Results Compared with preoperative visual acuity,16(64%)of 25 eyes had a best corrected visual acuity of ≥0.6 at the last follow-up(0.1～1.0)(P＜0.01);The mean preoperative intraocular pressure(IOP)was(23.01 ±2.63)mmHg which decreased to(14.02 ±2.05)mmHg(P＜0.05 ,P ＜0.01)after treatment.Conclusion The triple procedure is effective for treating patients with co-existing cataract and glaucoma,which could enhance the visual acuity,control IOP and reduce complications.

Objective To explore regional characteristics of road traffic injury in urban Beijing through analysis of the investigation by a single hospital. Methods Using Emergent Road Traffic Injury Questionnaire we designed, we performed a survey for 2 weeks on all the patients with road traffic injury who had sought medical care in the emergency department of our hospital. A comparison was made between them and other patients at the emergency department at the same period. Results From 8:00 on 25 April 2006 to 8:00 on 8 May 2006, 18 emergent cases of road traffic injury were treated. They accounted for 0.54% of all the emergency patients and 4.5% of all the trauma patients. They were 7 males and 11 females, with an average age of 48.3±14. 3 years. The average time to hospital was (26.8±14.4) rain. Seventeen cases of injury happened in the street, 1 in the highway;15 in Xicheng District, 1 in Haidian District, and 2 in the suburbs. They included 8 pedestrians, 9 riders, and 1 passenger. They were injured by 12 light vehicles, 2 heavy vehicles, 2 special vehicles, and 2 bicycles. Twelve patients had injury at extremity, 3 at spine, and 3 at multiple sites, They received treatment only in Emergency Department. No one was dead or hospitalized. Conclusions Contrary to common belief, road traffic injury patients seem to account for a small part of trauma patients and the injury tends to be moderate. Females and extremities may be more likely to get injured.