Acupuncture Points ; Acupuncture Therapy ; Aged ; Anterior Compartment Syndrome ; therapy ; Humans ; Male ; Tibia ; injuries

Objective To study the changes of ICAM-1 expression in transplanted lung at the early stage after rat pulmonary transplantation.Methods The expression level of ICAM-1 protein and its mRNA in rat transplanted lung after 4 h of ventilation and reperfussion were detected by using immunohisto chemical method(SABC method)and semiquantitative reverse transcriptase polymerase reaction method in the experimental group(n=6).When donor lungs had been flushed with low potassium solution(LPDS) and preserved in 4.C LPDS for 12 h,left orthotopic pulmonary transplantation was performed.In control group (n=6), left pulmonary artery,pulmonary vein and bronchi were fully freed to be naked.Immunohistochemical test results were recorded in negative(-),suspicion(±),faint positive(+),positive(++)and intensive positive(+++).PCR products were separated by agrose gels and the density of the bands were determined by density scanning.Results The stained color of alveolar epithelial cells and pulmonary vascular endothdial cells in the transplanted lung was significantly more intensive in experimental group than in control group(P＜0.01) and the relative density values(ICAM-1/βactin)were also significandy higher in experimental group(0.837±0.044) than in control group (0.442±0.037),P＜0.01. Conclusions ICAM-1 expression in the transplanted lung was up-regulated in the early stage after pulmonary transplantation,which was related with the enhancement of ICAM-1 mRNA.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

The taxol s effect of inducing apoptosis is mainly related to its ability to promote tubulin polymerization and prevent tubulin chain depolarization. Drug resistance is the main restriction factor for clinical usage of taxol. Some basic and clinical studies support that poor response to taxol and poor prognosis are related to overexpression of β tubulin Ⅲ.

Objective To explore the efficacy of ligustrazine combined with chemotherapy in patients with relapsed or refractory NHL and the relationships with P-gp expression. Methods 60 Patients were randomized into reversal agent group (ligustrazine + chemotherapy) and control group (chemotherapy) and the outcome was analysed. P- gp expression was analysed by flow cytometry. Subgroup analysis was conducted a ccording to P-gp expression. Results 56 patients could be evaluated. Progression -free survival (PFS) showed no statistical diference in two groups ( P = 0.065 1 ) ; reversal agent group had a higher overall response rate (ORR) than control group(P=0. 048).Subgroup analysis showed 41 patients had P-gp( +). The complete remission rate and complete remission uncertain rate of patients with P-gp( + ) in reversal agent group and control group were 33. 33% and 8. 70% , respectively, reversal agent group had a significant advantage ( P = 0. 048). Patients with P-gp( + ) in reversal agent group had a higher ORR than control group (61. 11% vs 26. 09% ,P =0.024). Kaplan-Meier survival curve and Log-rank test showed patients with P-gp( + ) in reversal agent group had longer PFS than control group (P =0.046 4). Blood pressure in a small number of patients received ligustrazine therapy decreased,and mitigated after reducing the dose. Conclusion Ligustrazine combined with chemotherapy can elevate response rate,prolong PFS with manageable toxicity and correlate to P-gp expression in relapsed or refractory NHL

Postoperative atrial fibrillation (POAF) is the most common complication after cardiac surgery, which leads to hemodynamics disorder, prolongs hospitalization and increases mortality. The underlying mechanisms behind POAF are associated with cardiomyocyte injury, sympathetic activation and inflammation. The prediction of POAF is based on correlated index from pre-, intra-and post-surgery. POAF can be treated by rhythm and heart rate control as well as and antithrombotic therapy.

The blood-brain barrier (BBB) exerts its central nervous system (CNS) protective function as it hinders the delivery of diagnostic and therapeutic agents to the brain. With the development of nanotechnology during the last thirty years, the nanocarriers for delivering drugs make it possible to transport drugs across the BBB. The brain-targeted drug delivery system usually consists of two parts: nanocarriers and brain-targeted strategies. In this review, several kinds of nanocarriers are introduced for brain-targeted drug delivery. We focus on several possible strategies for brain-targeting and comment on their advantages and disadvantages in application.

This paper reported TLC identification of various Chinese medicines in Renshensinitang Oral Liq- uid,limit dose detection and content determination of aconitine.These methods could be available for internal quality control of Renshensinitang Oral Liquid.

Objective To understand the characteristics of malaria endemic situation in Dongtai City from 1999 to 2013. Methods The data of diagnosed malaria cases and related information of prevention and treatment were collected and analyzed with the epidemiological method. Results From 1999 to 2013 there were 27 malaria cases in total and among which 2 cases were local-infected with Plasmodium vivax 10 cases were imported P. vivax infections 13 cases were imported P. falciparum infections and 1 case was a mixed infections P. falciparum P. vivax and P. ovale . The imported cases accounted for 92.59%and local-infected cases accounted for 7.41%. There were no local-infected cases after 2003 and all the cases were overseas-im-ported. The main first-diagnosis points were town hospitals and village health stations accounting for 33.33%and 33.33%re-spectively. Totally 62.96%of cases visited the clinic in 1 day after the attacking the median of time-interval from the attacking to visiting the clinic was 1 day the median of time-interval from the attacking to confirmed diagnosis was 3 days and the max time from the attacking to confirmed diagnosis was 33 days. Conclusion The malaria cases are mainly imported cases in Dong-tai City from 1999 to 2013 and therefore the key of eliminating malaria should be the monitoring of the retuned exporting la-bors.

OBJECTIVE:To put forward strategies to evolve the current drug bidding system.METHODS:The objective and efficacy of the drug bidding system was analyzed;and the reasons for the poor efficacy of the system were analyzed using the information asymmetry theory in economics,and the system was compared with its US counterpart.RESULTS & CONCLUSIONS:The drug bidding system in China should adopt such model in which the payers are chosen as agency and charge from the entrusting party.It is a systematic project to standardize drug circulation channels,reduce drug price and reduce the burden of patient,which can't be tackled by drug bidding system only.