Objective To explore the clinical efficacy and safety of mouth three oxide inorganic polymer under a microscope to repair the pulp cavity perforation.Methods 120 patients with medullary cavity perforation, caries origin were selected as the research subjects,they were randomly divided into oral microscope inorganic oxide polymer repair the pulp cavity perforation of the experimental group and the resin inlay repair the pulp cavity perfora-tion of the control group,each group had 60 cases.The postoperative occlusion pain,swollen gums and loose,fistula formation,the incidence of chewing function not completed and X -ray curtate alveolar bone density decrease the shadow changes were compared between the two groups.Results The incidence rates of bite pain,swollen gums, loosening of the observation group were 5.00%,3.33%,6.67%,which of the control group were 11.60%,13.33%, 18.33%,the differences between two groups were significant (χ2 =5.49,P <0.05;χ2 =4.76,P <0.05;χ2 =4.23, P <0.05).The fistula formation,chewing function not completed,X -ray curtate alveolar bone density to reduce shadow expand of the observation group were 3.33%,5.00%,5.00%,which of the control group were 10.00%, 30.00%,40.00%,the differences between the two groups were significant (χ2 =5.34,P <0.05;χ2 =4.12,P <0.05;χ2 =4.56,P <0.05).Conclusion The clinical curative effect of oral three oxide inorganic polymer under a microscope to repair the pulp cavity perforation is outstanding,compared with the resin inlay repair the pulp cavity perforation has many advantages,it can be used as a kind of ideal method used in the clinical work.

Objective To evaluate the effects of geraniol(GOH) on neointima hyperplasia in rat carotid artery balloon injury model, and explore the potential molecular mechanisms associated with this effect. Methods Totally 20 male Sprague-Dawley (SD) rats were randomly divided into sham operation group (without balloon injury), control group (with balloon injury), low concentration group (with 50 mg/kg GOH intervention after balloon injury) and high concentration group (with 200 mg/kg GOH intervention after balloon injury). The intima to media (I/M) area ratio of neointima was measured by hematoxylin- eosin (HE) staining. The expression of proliferating cell nuclear antigen (PCNA) was measured by immunohistochemical staining at 14th day after operation. As the marker of oxidative stress, the levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured by enzyme linked inmmnosorbent assay (ELISA). Results The I/M ratio, IOD, 8-OHdG and MDA values were increased in control group compared with those of sham group. The I/M ratio, IOD and 8-OHdG values were reduced in low concentration group compared with those of control group. But there was no significant difference in MDA level between low concentration group and control group. The I/M ratio, IOD, 8-OHdG and MDA values were significantly reduced in high concentration group compared with those of control group, which showed a more significant inhibitory effect than that of low concentration group (P<0.05). Conclusion GOH could attenuate balloon iniury induced neointima hyperplasia, which might be related to its effect on inhibiting expression of PCNA and decreasing oxidative stress.

Aim To purify monoclonal antibody in mouse ascites by a modified membrane affinity chromatography(MAC). Methods Human serum albumin HSA was adsorbed on the zeta-bind membrane (ZBM), a positively charged nylon membrane filter. Then the mouse aecites containing mAbs were filtrated through the ZBM to cause the mAbs bind to HSA adsorbed on ZBM. Finally,the purified mAbs were dissociated from the ZBM with guanidine hydrochloride solution. Results A sheet of ZBM(diameter 50mm) absorbed with HSA filtrated by 10 mL ascites could reach its maximum mAb binding capacity after two rounds of re-fitration. The dissociation of mAb from ZBM need only one round of filtration of dissociating solution. The purified mAb displayed a single band after PAGE. Sensitivity of detedcting HSA with purified mAbs was 20-fold higher than that with unpurified ascites in dot innunogold filtration assay(DIGFA). Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.

The curriculum,as the core of school education,is the intermediary of turning educational thoughts,ideas,purposes and aims into concrete educational practice.Therefore,the key question of the reform of nursing education is the reform of its curriculum which should accord with the principles of sticking to humanism,stressing the characteristics of nursing speciality,strengthening the training objectives and paying attention to the fostering of the students qualities and abilities.

Objective:To test Jiang Guangrong's(2005)three-dimension model on the depth of counseling process.Methods:In this process-outcome research,28 sessions in a university counseling center were videotaped and coded in terms of the model.Structure Equation Model(SEM)was used to examine the relations between the coding and counselors and clients' evaluation of the session depth.Results:(1)Clients and counselors evaluated differently on the session depth,and clients' evaluation was more positive than counselors' [(4.95±1.12)vs.(4.23±0.94),P<0.05];(2)The model was significantly correlated with counselors' session depth evaluation(path coefficient = 0.55,P<0.01),but not with the clients';(3)The session curves describing each dimension changing over time had different patterns in sessions of different depth level,through which more about the counseling process could be known.Conclusion:The three-dimension model on the depth of counseling process has got some empirical evidences.

This study was set up to observe the changes in stress and immune functions in upper abdominal surgery after general anesthesia.In thirty elective upper abdominal operation patients,general anesthesia was induced with propofol,fentanyl,scoline and maintained with isoflane and atrumium. Catecholamines(CA),cortisol,interleukin-2(IL-2) and soluble interleukin-2 receptor(sIL-2R) were measured before anesthesia,after induction of anesthesia,at the end of operation,and on the first and third postoperative days.The results showed that epinephrine(E),norepinephrine(NE) concentration increased at the end of operation and on the first postoperative day(P<0.01)，Cortisol concentration decreased after anesthesia induction(P<0.05)，IL-2 concentration decreased at the end of operation(P<0.05)，and sIL-2R lever did not change.The investigation suggested that general anesthesia with propofol,fentanyl and isoflane can suppress CA and cortisol increase resulting from induction of anesthesia. There was no change in IL-2 level.However it can not suppress the increase of stress hormones and decrease of IL-2 due to operation.The stress response correlate with immune functions.

Objective To research the clinical value of mechanical ventilation in the treatment of acute left ventricular failure.Methods Of 74 case with acute left ventricular failure,26 cases recevived routine treatment including nasal-catheter inhale of oxygen or veil inhale of oxygen and inotropic agent,diuretic,and dilation as well as sedative while the mechanical ventilation group were treated only with mechanical ventilation depending on the condition.The clinical indications,parameters of blood gas analysis and hemodynamies were compared before and after treatment.Results 18 cases from routine treatment group died(70.9%)and 5 died in mechanical ventilation group (10.4%).There was significant difference in heart rates (HR),central venous pressure (CVP),the mean arterial blood pressure (MAP) and some parameters of blood gas analysis before and after the treatment (P<0.05 or P

Objective To evaluate the effect of combination of phacoemulsification and trabeculectomy on patients with cataract and glaucoma.Methods 19 patients(23 eyes)with co-existing glaucoma and cataract were treated with triple procedure by means of scleral tunnel flap.The mean follow-up period was(15.8 ±3.5)months.Results Compared with preoperative visual acuity,16(64%)of 25 eyes had a best corrected visual acuity of ≥0.6 at the last follow-up(0.1～1.0)(P＜0.01);The mean preoperative intraocular pressure(IOP)was(23.01 ±2.63)mmHg which decreased to(14.02 ±2.05)mmHg(P＜0.05 ,P ＜0.01)after treatment.Conclusion The triple procedure is effective for treating patients with co-existing cataract and glaucoma,which could enhance the visual acuity,control IOP and reduce complications.

This study was set up to observe the changes in stress and immune functions in upper abdominal surgery after general anesthesia.In thirty elective upper abdominal operation patients,general anesthesia was induced with propofol,fentanyl,scoline and maintained with isoflane and atrumium. Catecholamines(CA),cortisol,interleukin-2(IL-2) and soluble interleukin-2 receptor(sIL-2R) were measured before anesthesia,after induction of anesthesia,at the end of operation,and on the first and third postoperative days.The results showed that epinephrine(E),norepinephrine(NE) concentration increased at the end of operation and on the first postoperative day(P

Long noncoding RNA (lncRNA) refers to RNA transcripts with more than 200 nucleotides in length, which plays regulatory roles directly in the form of RNA. Their functions are affected by their secondary structures, post transcriptional modification and so on. LncRNA can be classified to various subtypes with complicated mechanisms. In human-induced-pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), dynamic expression of some lncRNAs is detected during the neuronal differentiation, and a series of lncRNAs associated closely with the development process of the central nervous system (CNS) are found. This review summarizes the molecular biological properties of lncRNA and their regulatory functions in the development of the CNS, which is helpful to reveal the mechanisms of the pathogenesis and progression of certain neurological diseases and results in innovation of their diagnosis and treatment.