China ; Emigration and Immigration ; Immunization Programs

Objective To construct an efficient SALL4 shRNA vector and transfect it into THP-1 cells for investigating the effect of SALL4 in leukemia. Methods Four SALL4-specific siRNA to aim at different SALL4 mRNA target sites and a negative control siRNA were designed, and pGPU6/GFP/Neo/SALL4 shRNA vectors were constructed. THP-1 cells were transfected and the expression of SALL4 in shRNA detected, and blank control and negative control were also designed. Results The results of real time quantitive PCR and Western blotting both exhibited that the interference effect of pGPU6/GFP/Neo/SALL4 shRNA-B vector was optimal targeting to mRNA-1122 target site and down-regulated the expression of SALL4 more significant(P <0.05). Conclusion Successfully construction of SALL4 siRNA vector by choosing SALL4 shRNA-B would be useful to accomplish study of SALL4.

Objective To investigate the effects of angiotensinⅡ (AngⅡ) on the Apelin mRNA and APJ mRNA expression in pulmonary microvascular endothelial ceils (PMVECs) in rats. Methods Pulmonary microvascular endothelial ceils obtained from 24 h old neonatal SD rats were cultured in DMEM liquid culture medium. The 2nd-4th generation PMVECs were inoculated on 6-well plates (5×105). The experiment was performed in two parts. In part Ⅰ different concentration of AngⅡ 0, 10-9, 10-8, 10-7, 10-6mol/L (group Ⅰ- Ⅴ) were added into the PMVECs. The expression of Apelin mRNA and APJ mRNA was determined at 24 h after addition of Ang Ⅱ by RT-PCR. In part Ⅱ the cells were exposed to 10-7 mol/L Ang Ⅱ. The expression of Apelin mRNA and APJ mRNA was determined immediately and at 1, 6, 12, 24, 48 h(group Ⅰ-Ⅵ) after addition of Ang Ⅱ by RT-PCR. Results In part Ⅰ Apelin mRNA expression was significantly higher in group Ⅱ (Ang Ⅱ 10-9 mol/L) but lower in group Ⅲ-Ⅴ (AngⅡ 10-8, 10-7, 10-6 mol/L) than in group Ⅰ (control, Ang Ⅱ 0 mol/L). The APJ mRNA expression was significantly lowered in group Ⅱ-Ⅴ in a dose-dependent manner as compared with control group (group Ⅰ). In partⅡ beth Apelin mRNA and APJ mRNA expression exhibited a bi-phasic response to AngⅡ 10-7 mol/L, increased at first and was then decreasing with time. The Apelin mRNA and APJ mRNA expression reached the peak at 1 h of incubation with Ang Ⅱ respectively. Conclusion Ang Ⅱ decreases both Apelin mRNA and APJ mRNA expression in PMVECs in a dose and time dependent manner. The down-regulation of Apelin mRNA and APJ mRNA expression may be involved in the mechanism of injury to PMVECs.

Objective To evaluate the statues of P-selectin and chang of D-dimer in vivo in patients with deep venous thrombosis(DVT)and to observe how to vary under interneving of medicines.Methods P-selectin and D-dimer of fourty patients and twenty normal subjects were fluo-rescencelabled with its corresponding monoclonal antibodies by flow cytometry(FCM)and immune method respectively.Results In the early stage,P-selectin and D-dimer of patients with DVT is higher than that in normals,and they significantly decreased in different time after patients were treated.Compared with patients who were used on-sodium ozagrel,patients used sodium ozagrel have different P-selectin(P＜0.05),but D-dimer was similar(P＞0.05)after fourteen days.One month later,P-selectin and D-dimer of DVT patients were lower.However,the positive rate of P-selectin of DVT was still higher than normal subjects.Conclusions The platelet has been actived in vivo in patients with DVT,so do fibrinolysis.Sodium ozagrel can decrease the action of platelet.P-selectin and D-dimmer may be used in diagnose.Post-discharge patients are still high-risk group and must be followed-up regularly.

OBJECTIVE： To discuss the selectivity of enatiomers of clinically used asymmetric drugs.METHODS： To exsamplify this issue in respect to pharmacokinetic characteristics,pharmacodynamic properties and clinical application of asymmetric drugs.RESULTS ＆ CONCLUSION： The enatiomers of asymmetric drugs assume complex pharmacokinetic and pharmacodynamic characteristics.Thorough study of enatiomers of asymmetric drugs will exert great influence on rational use of asymmetric drugs.

Percutaneous balloon mitral valvuloplasty (PMV) was performed in 100 cases with rheumatic mitral stenosis. In 94 ceases who successfully underwent the procedure, the mean left atrial pressure lowered from 22. 77?0. 84mmHg to 14. 40?0. 57 mmHg(P

Objection: To study the characteristics of shame of patients with neurosis. Method: 52 patients with nuclear neurosis were evaluated by SCL-90, Shame Scale, and Children Trauma Questionnaire. The control group (n=30) was evaluated just by Shame Scale. Results:The total score of Shame Scale and the subscores of each factor were all higher in neurotic patients than in control group (total score 69.0?20.4/48.7?10.2, personality 29.4?9.7/19.8?5.8, behavior 23.5?6.5/18.0?4.2, body 8.3?3.4/5.7?2.2, family 7.7?3.9/5.2?1.7, t=3.15~5.09,P=0.001or

10%～20% of acute clinical sporatic hepatitis is hepatitis E (HE). HE has a severe diaease low mortality in general population of about 0.5～1.5%. It has been reported as severe disease and the mortality increased to 20% in pregnant women and old people. China is high-epidemic for hepatitis E virus (HEV). HEV is mainly transmitted through faecaloral route,data also showed that HEV could be transferred through blood donation. The epidemiology,diagnostics and vaccine development of HE is reviewed in this paper.

The review summarized the diagnostic strategy of ischemic cerebrovascular diseases and introduced some progress in clinic.As ischemic cerebrovascular diseases were highly heterogeneous,the review analyzed their characteristics step by step.Through the primary diagnosis,etiological diagnosis,evaluation of risk factors and factor analysis of the whole body,the review describe the diagnostic program of the diseases and provide suggestion about the individually therapy and prevention.

In the present study,the trachea of 17 new borns and 10 animals, including rabbits, dogs and monkeys were used. The neural elements were visualized by Cajal-Faworsky silver impregnation and osmic acid techniques. Specimens were cut in to sections by freezing microtome and stained by Gros—Bielchowsky method.Branches from the recurrent laryngeal and vagus nerves pass to the trachea,dividing and rejoining to form a mesh-like plexus outer to the cartilage. Fibers of the posterior parts of the plexus appear as a well-defined longitudinal chain of nerves. The branches from this plexus re-form a primary, secondary and tertiary plexuses in the membrane of cartilage plates, in the muscle, the submucous regions and the mucosa.Parasympathetic effector neurons were mostly found in the posterior and lateral wall, on rare occasions the anterior wall.A profuse supply of predominantly unmyelinated fibres innervates the muscle,blood vessels and glands, and the plexuses supplying these structure apparently communicate with each other. They also contain some thinly myelinated fibres.In the layer of smooth muscle the nerve fibres give off fine collaterals and end in knob-like or arrowheaded swellings. The fibres of subepithelial plexuses branch rectangularly and pass between the epithelial cells. All these fibers terminate in knobs which lie among the deep epithelial cells.