China ; Emigration and Immigration ; Immunization Programs

Objective To construct an efficient SALL4 shRNA vector and transfect it into THP-1 cells for investigating the effect of SALL4 in leukemia. Methods Four SALL4-specific siRNA to aim at different SALL4 mRNA target sites and a negative control siRNA were designed, and pGPU6/GFP/Neo/SALL4 shRNA vectors were constructed. THP-1 cells were transfected and the expression of SALL4 in shRNA detected, and blank control and negative control were also designed. Results The results of real time quantitive PCR and Western blotting both exhibited that the interference effect of pGPU6/GFP/Neo/SALL4 shRNA-B vector was optimal targeting to mRNA-1122 target site and down-regulated the expression of SALL4 more significant(P <0.05). Conclusion Successfully construction of SALL4 siRNA vector by choosing SALL4 shRNA-B would be useful to accomplish study of SALL4.

Developmental Disabilities ; diagnosis ; etiology ; genetics ; Humans ; Nervous System Malformations ; diagnosis ; etiology ; genetics

Objective To evaluate the statues of P-selectin and chang of D-dimer in vivo in patients with deep venous thrombosis(DVT)and to observe how to vary under interneving of medicines.Methods P-selectin and D-dimer of fourty patients and twenty normal subjects were fluo-rescencelabled with its corresponding monoclonal antibodies by flow cytometry(FCM)and immune method respectively.Results In the early stage,P-selectin and D-dimer of patients with DVT is higher than that in normals,and they significantly decreased in different time after patients were treated.Compared with patients who were used on-sodium ozagrel,patients used sodium ozagrel have different P-selectin(P＜0.05),but D-dimer was similar(P＞0.05)after fourteen days.One month later,P-selectin and D-dimer of DVT patients were lower.However,the positive rate of P-selectin of DVT was still higher than normal subjects.Conclusions The platelet has been actived in vivo in patients with DVT,so do fibrinolysis.Sodium ozagrel can decrease the action of platelet.P-selectin and D-dimmer may be used in diagnose.Post-discharge patients are still high-risk group and must be followed-up regularly.

OBJECTIVE： To discuss the selectivity of enatiomers of clinically used asymmetric drugs.METHODS： To exsamplify this issue in respect to pharmacokinetic characteristics,pharmacodynamic properties and clinical application of asymmetric drugs.RESULTS ＆ CONCLUSION： The enatiomers of asymmetric drugs assume complex pharmacokinetic and pharmacodynamic characteristics.Thorough study of enatiomers of asymmetric drugs will exert great influence on rational use of asymmetric drugs.

Emergency Treatment ; methods ; Humans ; Seizures ; therapy ; Status Epilepticus ; therapy

Humans ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; epidemiology ; virology

Background:Gastric cancer is the result of comprehensive interactions among Helicobacter pylori(Hp)infection, environmental factors and host genetic factors. It has been demonstrated that rs2294008 polymorphism in prostate stem cell antigen(PSCA)gene is associated with increased risk of non-cardia gastric cancer. Aims:To investigate the relationship between PSCA rs2294008 polymorphism and precursors of gastric cancer. Methods:A total of 398 patients with gastric intestinal metaplasia( n = 328)and intraepithelial neoplasia( n = 70)from Nov. 2009 to Nov. 2015 at the Qingdao Municipal Hospital were enrolled,and 416 healthy subjects were served as controls. Genotype of PSCA rs2294008 was determined by direct DNA sequencing of PCR products,and Hp infection was examined by rapid urease test. Results:Significant difference in frequencies of CC,CT and TT genotypes of PSCA rs2294008 was observed between case and control groups(P = 0. 011);the frequency of TT genotype was significantly higher in case group than in control group (16. 3% vs. 9. 4% ,P = 0. 003). Compared with individuals carrying CC genotype,TT genotype carriers were found to be associated with a higher risk of precursors of gastric cancer(oR = 1. 840,95% CI:1. 174 ~ 2. 886). Taken individuals negative for Hp infection and carrying C allele(CC + CT)as reference,risk of precursors of gastric cancer was significantly increased by Hp infection(oR = 2. 389,95% CI:1. 799 ~ 3. 173)and Hp infection in combination with TT genotype (OR = 3. 335,95% CI:1. 935 ~ 5. 749),whereas TT genotype alone only slightly increased the risk(OR = 1. 783,95%CI:0. 900 ~ 3. 530). Conclusions:PSCA rs2294008 polymorphism is significantly associated with susceptibility to precursors of gastric cancer and Hp infection might further increase the risk in TT carriers.

An online questionnaire to 103 professionals of traditional Chinese medicine (TCM) was conducted to acquire their recognition and understanding of international communicative competence. The results showed that foreign language communicative competence and solid medical knowledge are considered the most important qualities while the importance of global vision, strategic thinking, cross-cultural awareness/sensitivity and communicative strategy is comparatively underestimated. It was proposed that domestic TCM universities and colleges strive to improve the soft skills for TCM professionals and cultivate more interdisciplinary TCM talents armed with professional expertise, foreign language communicative competence as well as international and multicultural awareness.

Objective The study aimed to evaluate the incidence rate and risk factors of early post-mastectomy pain syndrome (EPMPS) after surgery for breast cancer.Methods A retrospective study was carried out in 231 female patients with breast cancer who appeared EPMPS after surgery in the First Hospital of China Medical University from September 2012 to September 2014.They were followed-up for 3 months.Results Among 231 female patients with breast cancer,41.1％ (95/231) suffered from EPMPS.The incidence of EPMPS in women of 40-59 years old was 66.3％ (63/95),significantly higher than other age groups,RR=l.83,P＜0.05.Those who were submitted to axillary lymph node dissection and those without preservation of intercostobrachial nerve had shown a significantly increased risk of EPMPS compared with those with sentinel lymph node biopsy plus no surgery and preservation of intercostobrachial nerve patients (81 cases vs.14 cases,41 cases vs.18 cases,x2=19.921,P＜0.01;x2=11.045,P＜0.05).Conclusions The frequency of EPMPS in the middle-age women is high and possible explanations are unclear.Surgery of axilla affects the incidence of EPMPS,reassurance is key to coping with EPMPS in nursing and rehabilitation.